KEGG   ENZYME: 1.14.14.192
Entry
EC 1.14.14.192              Enzyme                                 
Name
taxoid 1beta-hydroxylase;
taxane 1beta-hydroxylase;
CYP725A23-1;
T1betaOH
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
10-deacetyl-1-deoxybaccatin III,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (1beta-hydroxylating)
Reaction(IUBMB)
10-deacetyl-1-deoxybaccatin III + [reduced NADPH---hemoprotein reductase] + O2 = 10-deacetylbaccatin III + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R13494]
Reaction(KEGG)
R13494
Substrate
10-deacetyl-1-deoxybaccatin III [CPD:C23034];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
10-deacetylbaccatin III [CPD:C11700];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome-P-450 (heme thiolate) enzyme involved in paclitaxel (taxol) biosynthesis. Isolated from the plant Taxus baccata (English yew).
History
EC 1.14.14.192 created 2025
Pathway
ec00904  Diterpenoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Reference
1  [PMID:37897038]
  Authors
Zhang Y, Wiese L, Fang H, Alseekh S, Perez de Souza L, Scossa F, Molloy J, Christmann M, Fernie AR.
  Title
Synthetic biology identifies the minimal gene set required for paclitaxel biosynthesis in a plant chassis.
  Journal
Mol Plant 16:1951-1961 (2023)
DOI:10.1016/j.molp.2023.10.016
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.192
IUBMB Enzyme Nomenclature: 1.14.14.192
ExPASy - ENZYME nomenclature database: 1.14.14.192
BRENDA, the Enzyme Database: 1.14.14.192
LinkDB

KEGG   REACTION: R13494
Entry
R13494                      Reaction                               
Name
10-deacetyl-1-deoxybaccatin III,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (1beta-hydroxylating)
Definition
10-Deacetyl-1-deoxybaccatin III + [Reduced NADPH---hemoprotein reductase] + Oxygen <=> 10-Deacetylbaccatin III + [Oxidized NADPH---hemoprotein reductase] + H2O
Equation
C23034 + C03024 + C00007 <=> C11700 + C03161 + C00001
Enzyme
1.14.14.192
Pathway
rn00904  Diterpenoid biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.14  With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.14.192
     R13494  10-Deacetyl-1-deoxybaccatin III + [Reduced NADPH---hemoprotein reductase] + Oxygen <=> 10-Deacetylbaccatin III + [Oxidized NADPH---hemoprotein reductase] + H2O
LinkDB

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