KEGG ENZYME: 1.14.14.69

ENZYME: 1.14.14.69

Entry

EC 1.14.14.69               Enzyme

Name

ent-cassadiene hydroxylase;
CYP71Z7

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

ent-cassa-12,15-diene,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (ent-3beta-hydroxycassa-12,15-dien-2-one-forming)

Reaction(IUBMB)

ent-cassa-12,15-diene + 3 [reduced NADPH---hemoprotein reductase] + 3 O2 = ent-3beta-hydroxycassa-12,15-dien-2-one + 3 [oxidized NADPH---hemoprotein reductase] + 4 H2O (overall reaction) [RN:R11981];
(1a) ent-cassa-12,15-diene + [reduced NADPH---hemoprotein reductase] + O2 = ent-cassa-12,15-dien-2beta-ol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R11982];
(1b) ent-cassa-12,15-dien-2beta-ol + [reduced NADPH---hemoprotein reductase] + O2 = ent-cassa-12,15-dien-2-one + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN:R11983];
(1b') ent-cassa-12,15-dien-2beta-ol + [reduced NADPH---hemoprotein reductase] + O2 = ent-cassa-12,15-diene-2beta,3beta-diol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R11985];
(1c) ent-cassa-12,15-dien-2-one + [reduced NADPH---hemoprotein reductase] + O2 = ent-3beta-hydroxycassa-12,15-dien-2-one + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R11984];
(1c') ent-cassa-12,15-diene-2beta,3beta-diol + [reduced NADPH---hemoprotein reductase] + O2 = ent-3beta-hydroxycassa-12,15-dien-2-one + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN:R11986]

Reaction(KEGG)

R11981 R11982 R11983 R11984 R11985 R11986

Substrate

ent-cassa-12,15-diene [CPD:C18226];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007];
ent-cassa-12,15-dien-2beta-ol [CPD:C21827];
(1b') ent-cassa-12,15-dien-2beta-ol;
ent-cassa-12,15-dien-2-one [CPD:C21828];
(1c') ent-cassa-12,15-diene-2beta,3beta-diol

Product

ent-3beta-hydroxycassa-12,15-dien-2-one [CPD:C21826];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001];
ent-cassa-12,15-dien-2beta-ol [CPD:C21827];
ent-cassa-12,15-dien-2-one [CPD:C21828];
ent-cassa-12,15-diene-2beta,3beta-diol [CPD:C21829]

Comment

A cytochrome P-450 (heme-thiolate) protein isolated from the plant Oryza sativa (rice) that is involved in phytocassanes biosynthesis. Depending on the order of activities, the enzyme may form either ent-cassa-12,15-dien-2-one or ent-cassa-12,15-diene-2beta,3beta-diol as an intermediate.

History

EC 1.14.14.69 created 2018

Pathway

ec00904

Diterpenoid biosynthesis

ec01110

Biosynthesis of secondary metabolites

Orthology

K21291

ent-cassadiene hydroxylase

Genes