KEGG   ENZYME: 1.14.14.98
Entry
EC 1.14.14.98               Enzyme                                 
Name
protopine 6-monooxygenase;
protopine 6-hydroxylase;
CYP82N2 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
protopine,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (6-hydroxylating)
Reaction(IUBMB)
protopine + [reduced NADPH---hemoprotein reductase] + O2 = 6-hydroxyprotopine + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R04699]
Reaction(KEGG)
R04699
Substrate
protopine [CPD:C05189];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
6-hydroxyprotopine [CPD:C05190];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein involved in benzophenanthridine alkaloid synthesis in higher plants.
History
EC 1.14.14.98 created 1999 as EC 1.14.13.55, transferred 2018 to EC 1.14.14.98
Pathway
ec00950  Isoquinoline alkaloid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K21693  protopine 6-monooxygenase
Genes
PSOM: 113330723 113357146
Reference
1
  Authors
Tanahashi T, Zenk MH.
  Title
Elicitor induction and characterization of microsomal protopine-6-hydroxylase, the central enzyme in benzophenanthridine alkaloid biosynthesis.
  Journal
Phytochemistry 29:1113-1122 (1990)
Reference
2  [PMID:22421633]
  Authors
Takemura T, Ikezawa N, Iwasa K, Sato F
  Title
Molecular cloning and characterization of a cytochrome P450 in sanguinarine biosynthesis from Eschscholzia californica cells.
  Journal
Phytochemistry 91:100-8 (2013)
DOI:10.1016/j.phytochem.2012.02.013
  Sequence
[ag:BAK20464]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.98
IUBMB Enzyme Nomenclature: 1.14.14.98
ExPASy - ENZYME nomenclature database: 1.14.14.98
BRENDA, the Enzyme Database: 1.14.14.98
CAS: 128561-60-4
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