KEGG   ENZYME: 1.14.15.31
Entry
EC 1.14.15.31               Enzyme                                 

Name
2-hydroxy-5-methyl-1-naphthoate 7-hydroxylase;
NcsB3
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
2-hydroxy-5-methyl-1-naphthoate,reduced ferredoxin:oxygen oxidoreductase (7-hydroxylating)
Reaction(IUBMB)
2-hydroxy-5-methyl-1-naphthoate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 = 2,7-dihydroxy-5-methyl-1-naphthoate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O [RN:R10796]
Reaction(KEGG)
R10796
Substrate
2-hydroxy-5-methyl-1-naphthoate [CPD:C20856];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
2,7-dihydroxy-5-methyl-1-naphthoate [CPD:C20857];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein involved in the synthesis of neocarzinostatin in the bacterium Streptomyces carzinostaticus.
History
EC 1.14.15.31 created 2014 as EC 1.14.99.49, transferred 2018 to EC 1.14.15.31
Pathway
ec01059  Biosynthesis of enediyne antibiotics
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K20420  2-hydroxy-5-methyl-1-naphthoate 7-hydroxylase
Genes
LBQ: CKQ53_05320
XOR: XOC_0085
XOZ: BE73_23900
XTN: FD63_13545
XTH: G4Q83_03485
MLO: mll6125
MERD: EB233_29925
NFA: NFA_22930 NFA_45180 NFA_53110
NFR: ERS450000_01804 ERS450000_04570 ERS450000_05413
NCY: NOCYR_0216 NOCYR_3413 NOCYR_4397 NOCYR_4398
SCO: SCO2884(SCE6.21) SCO7417(SC6D11.13c)
SMA: SAVERM_109(cyp1) SAVERM_1308(cyp6) SAVERM_3704(cyp17) SAVERM_3882(cyp19)
SBH: SBI_01881(cyp20) SBI_07324(cyp35) SBI_07588(cyp38)
SVE: SVEN_0422
SLE: sle_03480(sle_03480) sle_12110(sle_12110) sle_62170(sle_62170)
STRD: NI25_24330
SNF: JYK04_06995(ncsB3)
SHUN: DWB77_04303(ncsB3)
SCYG: S1361_02770(eryF1)
TCU: Tcur_1432
FAL: FRAAL1514
AMD: AMED_7339
AMN: RAM_37725
AMM: AMES_7228
AMZ: B737_7228
AOI: AORI_4218
AMI: Amir_3059
KAL: KALB_3732
ALO: CRK59466
SAQ: Sare_4949
 » show all
Reference
1  [PMID:20735485]
  Authors
Hang VT, Oh TJ, Yamaguchi T, Sohng JK
  Title
In vivo characterization of NcsB3 to establish the complete biosynthesis of the naphthoic acid moiety of the neocarzinostatin chromophore.
  Journal
FEMS Microbiol Lett 311:119-25 (2010)
DOI:10.1111/j.1574-6968.2010.02075.x
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.15.31
IUBMB Enzyme Nomenclature: 1.14.15.31
ExPASy - ENZYME nomenclature database: 1.14.15.31
BRENDA, the Enzyme Database: 1.14.15.31
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