KEGG ENZYME: 1.14.15.31

ENZYME: 1.14.15.31

Entry

EC 1.14.15.31               Enzyme

Name

2-hydroxy-5-methyl-1-naphthoate 7-hydroxylase;
NcsB3

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

2-hydroxy-5-methyl-1-naphthoate,reduced ferredoxin:oxygen oxidoreductase (7-hydroxylating)

Reaction(IUBMB)

2-hydroxy-5-methyl-1-naphthoate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 = 2,7-dihydroxy-5-methyl-1-naphthoate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O [RN:R10796]

Reaction(KEGG)

R10796

Substrate

2-hydroxy-5-methyl-1-naphthoate [CPD:C20856];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138];
H+ [CPD:C00080];
O2 [CPD:C00007]

Product

2,7-dihydroxy-5-methyl-1-naphthoate [CPD:C20857];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139];
H2O [CPD:C00001]

Comment

A cytochrome P-450 (heme-thiolate) protein involved in the synthesis of neocarzinostatin in the bacterium Streptomyces carzinostaticus.

History

EC 1.14.15.31 created 2014 as EC 1.14.99.49, transferred 2018 to EC 1.14.15.31

Pathway

ec01059

Biosynthesis of enediyne antibiotics

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K20420

2-hydroxy-5-methyl-1-naphthoate 7-hydroxylase

Genes

LBQ:	CKQ53_05320

ETP:	LU633_02190

XOR:

XOC_0085

XOZ:	BE73_23900

XTN:	FD63_13545

XTH:	G4Q83_03485

MLO:

mll6125

MERD:

EB233_29925

NFA:	NFA_22930 NFA_45180 NFA_53110

NFR:	ERS450000_01804 ERS450000_04570 ERS450000_05413

NCY:	NOCYR_0216 NOCYR_3413 NOCYR_4397 NOCYR_4398

NBR:	O3I_001245 O3I_016330 O3I_021950 O3I_027950

NNO:	NONO_c13610 NONO_c15520 NONO_c55450

NSL:	BOX37_14605 BOX37_23285

NSR:	NS506_02937 NS506_04941

NTP:	CRH09_09685 CRH09_15665 CRH09_18305

NOZ:	DMB37_06955 DMB37_22310 DMB37_37765

NOD:	FOH10_08035 FOH10_19640 FOH10_22545

NAH:	F5544_08475 F5544_18890

NAD:	NCTC11293_03171 NCTC11293_04204 NCTC11293_05655

NWL:	NWFMUON74_26820 NWFMUON74_41780 NWFMUON74_44890 NWFMUON74_51250 NWFMUON74_53680

NIE:	KV110_01240 KV110_15580 KV110_16390 KV110_18315 KV110_27215

NTC:	KHQ06_14085 KHQ06_15520 KHQ06_27165

NHU:	H0264_13770 H0264_14795

NYA:	LTV02_08580 LTV02_15325 LTV02_18310 LTV02_19085

NGP:	LTT66_00215 LTT66_00410 LTT66_00425 LTT66_07700 LTT66_28845

SCO:	SCO2884(SCE6.21) SCO7417(SC6D11.13c)

SALB:

XNR_0810 XNR_2289 XNR_2319

SMA:	SAVERM_109(cyp1) SAVERM_1308(cyp6) SAVERM_3704(cyp17) SAVERM_3882(cyp19)

SGR:	SGR_1085 SGR_3108 SGR_622

SGB:	WQO_20200 WQO_30190

SCB:	SCAB_20211 SCAB_32131

SSX:	SACTE_2263 SACTE_3804 SACTE_6025

SVL:	Strvi_2196 Strvi_7659

SHY:	SHJG_0095 SHJG_2299 SHJG_8229

SHO:	SHJGH_2064 SHJGH_7990

SRC:	M271_14635 M271_32985

SMAL:

SMALA_2464 SMALA_5783

SSOI:

I1A49_15785 I1A49_32635

SFA:	Sfla_0779 Sfla_2701 Sfla_4117 Sfla_5505

SBH:	SBI_01881(cyp20) SBI_07324(cyp35) SBI_07588(cyp38)

SVE:

SVEN_0422

SDV:	BN159_0797 BN159_1304

SALS:

SLNWT_4502 SLNWT_7122

STRP:

F750_1106 F750_2599 F750_4099 F750_6081

SFI:	SFUL_4231 SFUL_6410

SCI:	B446_03455

SLV:	SLIV_00215 SLIV_02225 SLIV_23265

SGU:	SGLAU_25545(cyp17) SGLAU_31595

SVT:	SVTN_04005 SVTN_35390

STRE:

GZL_05877 GZL_08215

SCW:	TU94_00970 TU94_03385

SLD:	T261_05751

SLC:	SL103_06415

SXI:	SXIM_32420

STRM:

M444_02645 M444_05420 M444_06720 M444_37900

STRC:

AA958_20030

SAMB:

SAM23877_0230(cyp107B) SAM23877_0787 SAM23877_0848 SAM23877_7443(cyp107B)

SPRI:

SPRI_0135 SPRI_0320 SPRI_4276 SPRI_7033 SPRI_7218

SCZ:	ABE83_03465 ABE83_04305 ABE83_15155

SCX:	AS200_25700 AS200_41585

SRW:	TUE45_00479 TUE45_00829(eryF) TUE45_01059(vdh_1)

STRF:

ASR50_18825

SLE:	sle_03480(sle_03480) sle_12110(sle_12110) sle_62170(sle_62170)

SPAV:

Spa2297_01655 Spa2297_12320

STRT:

A8713_01270 A8713_29885

SCLF:

BB341_11715 BB341_27285 BB341_27625 BB341_27875

SGS:	AVL59_26255 AVL59_26840 AVL59_28135 AVL59_32365

STSI:

A4E84_01955 A4E84_20615

SKY:	D0C37_00925 D0C37_05465 D0C37_13220 D0C37_13385

SALW:

CP975_15440 CP975_30160

SGM:	GCM10017557_33360 GCM10017557_56600

SPHW:

NFX46_12255 NFX46_15630

SNR:	SNOUR_00270 SNOUR_06910 SNOUR_27655 SNOUR_36945(cyp6) SNOUR_43680

SALU:

DC74_1310 DC74_3059 DC74_329

SALL:

SAZ_02095 SAZ_07045 SAZ_16330

SPLU:

LK06_000705 LK06_002680

STRD:

NI25_24330

SNW:	BBN63_00305 BBN63_09460 BBN63_21345

SAUO:

BV401_15850 BV401_32800

SSIA:

A7J05_00560 A7J05_19955 A7J05_33655 A7J05_36175

SVU:	B1H20_02120 B1H20_02150 B1H20_20375 B1H20_31005

SPUN:

BFF78_07355

SGV:	B1H19_14615

SLAU:

SLA_0018 SLA_0318 SLA_6823

SALJ:

SMD11_0702 SMD11_1711

SLX:	SLAV_06825(vdh1) SLAV_33495

STRO:

STRMOE7_13470

SFK:	KY5_0136c KY5_0744 KY5_3596 KY5_6077 KY5_8070c

SNZ:	DC008_00615 DC008_32125

SRJ:	SRO_1158 SRO_1505

SLK:	SLUN_00485 SLUN_15750 SLUN_38635

SGD:	ELQ87_01940 ELQ87_05105 ELQ87_36190

SQZ:	FQU76_13310 FQU76_22270 FQU76_26530 FQU76_32330

SCYA:

EJ357_35275 EJ357_42475 EJ357_45190

STIR:

DDW44_00810 DDW44_29175 DDW44_31375

SKA:	CP970_10575 CP970_24295 CP970_39280 CP970_43350

SGZ:	C0216_11955

SVN:	CP980_02070 CP980_05510

SNK:	CP967_02920 CP967_04280 CP967_14380 CP967_15145 CP967_22285

SHAW:

CEB94_02430 CEB94_20755

SRK:	FGW37_24875

SFIC:

EIZ62_05535 EIZ62_15365 EIZ62_18440 EIZ62_30720

SGAL:

CP966_34265

SSPO:

DDQ41_00860 DDQ41_21455 DDQ41_30005 DDQ41_30940

SVR:	CP971_10550 CP971_15130

SPAD:

DVK44_27765

SFY:	GFH48_22780 GFH48_36815 GFH48_38935

SAQU:

EJC51_22545 EJC51_36380

SGF:	HEP81_01125 HEP81_01693 HEP81_07958

SCAV:

CVT27_19390 CVT27_29455 CVT27_30230

SSEO:

D0Z67_03615 D0Z67_22675 D0Z67_28045

SBY:	H7H31_06885 H7H31_25020

SRIM:

CP984_15090 CP984_35310 CP984_38550

SSUB:

CP968_03305 CP968_05965 CP968_30745

SPHV:

F9278_38030

SCIN:

CP977_01840 CP977_03670 CP977_10085 CP977_31880

SFUG:

CNQ36_15300

SPAC:

B1H29_23075

STSU:

B7R87_00250 B7R87_01695 B7R87_12915 B7R87_21820 B7R87_26385

SCHA:

CP983_37555 CP983_41400

SGX:	H4W23_04555 H4W23_36190 H4W23_37305

SCOE:

CP976_22205 CP976_33635 CP976_40710

SBAT:

G4Z16_02345 G4Z16_08235 G4Z16_08740 G4Z16_19925

SSPB:

CP982_02885 CP982_39980

SNF:	JYK04_06995(ncsB3)

SPLA:

CP981_12975

SBRO:

GQF42_06550 GQF42_39935

STUI:

GCM10017668_35600 GCM10017668_55660 GCM10017668_67040

SATA:

C5746_23480

SCHF:

IPT68_16280

SHUN:

DWB77_04303(ncsB3)

SHAR:

HUT13_16700

SCYG:

S1361_02770(eryF1)

SFEU:

IM697_24500 IM697_25075

SLIA:

HA039_18790

SDW:	K7C20_12250 K7C20_23035

SVD:	CP969_00465 CP969_17840 CP969_20590

SCAL:

I6J39_02245 I6J39_02275 I6J39_20185 I6J39_30810

SAUH:

SU9_010515 SU9_021050 SU9_030625

SMOB:

J7W19_13355 J7W19_24670

SPRA:

CP972_03595 CP972_16040 CP972_28990

SPEU:

CGZ69_34815

SROI:

IAG44_02330 IAG44_03650 IAG44_38110

SXN:	IAG42_07270 IAG42_15655 IAG42_31110 IAG42_33875

SGJ:	IAG43_00205 IAG43_00935 IAG43_13315 IAG43_31565

SHK:	J2N69_03375 J2N69_15875

SANU:

K7396_04935 K7396_13960 K7396_24345

STRY:

EQG64_29730 EQG64_30775 EQG64_31580

SDD:	D9753_02285 D9753_32560

SACT:

DMT42_02400

SMAO:

CAG99_15930 CAG99_18330

SGOB:

test1122_06935

SINE:

KI385_15970

SLF:	JEQ17_15890 JEQ17_19930 JEQ17_43355 JEQ17_49110

SDEC:

L3078_35525 L3078_43210

SAOV:

G3H79_00240 G3H79_02610 G3H79_38355 G3H79_40300

SDUR:

M4V62_00890 M4V62_02955 M4V62_40990 M4V62_43370

SYAN:

NRK68_29320

SRUG:

F0345_16800 F0345_23695

SAKB:

K1J60_10440

STAA:

LDH80_34495 LDH80_35760

SNIG:

HEK616_08830 HEK616_79510

SVIO:

HWN34_00830 HWN34_04725 HWN34_12030 HWN34_12180

KSK:	KSE_03080 KSE_07770 KSE_19190 KSE_19210 KSE_42760 KSE_74300

KAB:	B7C62_00645 B7C62_20460 B7C62_32065

KAU:	B6264_05620 B6264_10165

KIT:	CFP65_4254 CFP65_5244 CFP65_5759 CFP65_7617

KIS:	HUT16_08105 HUT16_19695 HUT16_35895

STRI:

C7M71_026660

YIA:	LO772_22550

STRH:

GXP74_27745

ABRY:

NYE86_04560

TFU:	Tfu_1257 Tfu_1748 Tfu_2802 Tfu_2976

THAO:

NI17_007995 NI17_008210 NI17_017550 NI17_023955

TALX:

FOF52_03235 FOF52_08235 FOF52_14995 FOF52_16170

NDA:	Ndas_2750 Ndas_4555

NAL:	B005_0905 B005_1483 B005_2426 B005_5303

NEX:	NE857_03680 NE857_19420 NE857_19430 NE857_21025

NEC:	KGD82_10110 KGD82_25290

STRR:

EKD16_06410 EKD16_16105 EKD16_18695(vdh1) EKD16_18950(vdh2) EKD16_21840(vdh3) EKD16_23225

SNAH:

OUQ99_09545 OUQ99_10545

TCU:

Tcur_1432

AGRA:

AGRA3207_001012

SRO:	Sros_6983 Sros_8715

NOA:	BKM31_06905 BKM31_10805 BKM31_24015 BKM31_30205 BKM31_50570

NOW:	GBF35_14365 GBF35_31095 GBF35_34610

NCX:	Nocox_13485 Nocox_24380

NGN:	LCN96_23305 LCN96_30555 LCN96_32525 LCN96_34585 LCN96_50775

FAL:

FRAAL1514

AMD:

AMED_7339

AMN:

RAM_37725

AMM:

AMES_7228

AMZ:

B737_7228

AOI:

AORI_4218

AJA:	AJAP_17820

AMYC:

CU254_13715 CU254_41355

AMYB:

BKN51_28845 BKN51_28940 BKN51_41810

AAB:	A4R43_37295 A4R43_40390

AMYY:

YIM_37285 YIM_40790

AORI:

SD37_05155 SD37_23115

AROO:

NQK81_30325 NQK81_44935

ATHM:

L1857_20945

APRE:

CNX65_15545

AMI:

Amir_3059

SESP:

BN6_38980 BN6_40190 BN6_48460

SSYI:

EKG83_16920 EKG83_22955 EKG83_30320

KAL:

KALB_3732

KPHY:

AOZ06_23035 AOZ06_31150

AHM:	TL08_17105 TL08_17125

ACTI:

UA75_16455 UA75_20810 UA75_20825

ACAD:

UA74_15990 UA74_20305 UA74_20320

AHG:	AHOG_16510(eryF) AHOG_18165 AHOG_18180

ACTA:

C1701_20910

ALO:

CRK59466

ACTU:

Actkin_05259

STP:	Strop_2724

SAQ:

Sare_4949

MTEM:

GCE86_08090

VMA:	VAB18032_17045 VAB18032_29041

AIH:	Aiant_18010

PLAB:

C6361_26025 C6361_26075

PLAT:

C6W10_12775 C6W10_12825

PSUU:

Psuf_070200

CAI:	Caci_4185 Caci_5282

AEY:	CDG81_11605 CDG81_12515

» show all

Reference

1 [PMID:20735485]

Authors

Hang VT, Oh TJ, Yamaguchi T, Sohng JK

Title

In vivo characterization of NcsB3 to establish the complete biosynthesis of the naphthoic acid moiety of the neocarzinostatin chromophore.

Journal

FEMS Microbiol Lett 311:119-25 (2010)
DOI:10.1111/j.1574-6968.2010.02075.x

Sequence

[ag:AAM77997]

Other DBs

ExplorEnz - The Enzyme Database:

1.14.15.31

IUBMB Enzyme Nomenclature:

1.14.15.31

ExPASy - ENZYME nomenclature database:

1.14.15.31

BRENDA, the Enzyme Database:

1.14.15.31

LinkDB

DBGET integrated database retrieval system