KEGG   ENZYME: 1.14.15.43
Entry
EC 1.14.15.43               Enzyme                                 
Name
nocardicin C N-oxygenase;
nocL (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
nocardicin C,reduced ferredoxin:O2 oxidoreductase (nocardicin A-forming)
Reaction(IUBMB)
nocardicin C + 2 O2 + 4 reduced ferredoxin + 4 H+ = nocardicin A + 3 H2O + 4 oxidized ferredoxin (overall reaction) [RN:R10882];
(1a) nocardicin C + O2 + 2 reduced ferredoxin + 2 H+ = 2'-N-hydroxynocardicin C + H2O + 2 oxidized ferredoxin [RN:R13423];
(1b) 2'-N-hydroxynocardicin C + O2 + 2 reduced ferredoxin + 2 H+ = 2'-N,N-dihydroxynocardicin C + 2 oxidized ferredoxin [RN:R13424];
(1c) 2'-N,N-dihydroxynocardicin C = nocardicin A + H2O [RN:R13425]
Reaction(KEGG)
Substrate
nocardicin C [CPD:C17351];
O2 [CPD:C00007];
reduced ferredoxin [CPD:C00138];
H+ [CPD:C00080];
2'-N-hydroxynocardicin C [CPD:C22982];
2'-N,N-dihydroxynocardicin C [CPD:C22983]
Product
nocardicin A [CPD:C01941];
H2O [CPD:C00001];
oxidized ferredoxin [CPD:C00139];
2'-N-hydroxynocardicin C [CPD:C22982];
2'-N,N-dihydroxynocardicin C [CPD:C22983]
Comment
This cytochrome P-450 (heme-thiolate) enzyme, characterized from the bacterium Nocardia uniformis, is involved in the biosynthesis of the monolactam antibiotic nocardicin A.
History
EC 1.14.15.43 created 2024
Pathway
ec00261  Monobactam biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K19106  nocardicin C N-oxygenase
Genes
RHJSOYT95_26420
SPRFK4G22_25530
AMIAmir_4596
ACTIUA75_15350
ACADUA74_14780
ACTAC1701_23435
CROSN8J89_07200
Reference
1  [PMID:12105888]
  Authors
Kelly WL, Townsend CA
  Title
Role of the cytochrome P450 NocL in nocardicin A biosynthesis.
  Journal
J Am Chem Soc 124:8186-7 (2002)
DOI:10.1021/ja025926g
Reference
2  [PMID:15629944]
  Authors
Kelly WL, Townsend CA
  Title
Mutational analysis of nocK and nocL in the nocardicin a producer Nocardia uniformis.
  Journal
J Bacteriol 187:739-46 (2005)
DOI:10.1128/JB.187.2.739-746.2005
Other DBs
ExplorEnz - The Enzyme Database: 1.14.15.43
IUBMB Enzyme Nomenclature: 1.14.15.43
ExPASy - ENZYME nomenclature database: 1.14.15.43
BRENDA, the Enzyme Database: 1.14.15.43
LinkDB

DBGET integrated database retrieval system