KEGG ENZYME: 1.14.18.7

ENZYME: 1.14.18.7

Entry

EC 1.14.18.7                Enzyme

Name

dihydroceramide fatty acyl 2-hydroxylase;
FAH1 (gene name);
FAH2 (gene name);
plant sphingolipid fatty acid 2-hydroxylase

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With another compound as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

dihydroceramide,ferrocytochrome-b5:oxygen oxidoreductase (fatty acyl 2-hydroxylating)

Reaction(IUBMB)

a dihydroceramide + 2 ferrocytochrome b5 + O2 + 2 H+ = a (2'R)-2'-hydroxydihydroceramide + 2 ferricytochrome b5 + H2O [RN:R11003]

Reaction(KEGG)

R11003

Substrate

dihydroceramide [CPD:C12126];
ferrocytochrome b5 [CPD:C00999];
O2 [CPD:C00007];
H+ [CPD:C00080]

Product

(2'R)-2'-hydroxydihydroceramide [CPD:C21021];
ferricytochrome b5 [CPD:C00996];
H2O [CPD:C00001]

Comment

The enzyme, characterized from plants, catalyses the hydroxylation of carbon 2 of long- or very-long-chain fatty acids attached to sphinganine during de novo ceramide synthesis. The enzyme requires an external cytochrome b5 as the electron donor. The newly introduced 2-hydroxyl group has R-configuration. cf. EC 1.14.18.6, 4-hydroxysphinganine ceramide fatty acyl 2-hydroxylase.

History

EC 1.14.18.7 created 2015

Orthology

K19706

dihydroceramide fatty acyl 2-hydroxylase

Genes

AJM:

119059600

ATH:	AT2G34770(FAH1) AT4G20870(FAH2)

ALY:	9303944 9317424

CRB:	17877966 17888258

CSAT:

104718252 104722976 104731469 104781876 104786078 104792235

EUS:	EUTSA_v10017638mg EUTSA_v10026120mg

BRP:	103857549 103858439 103864297 103865353 103867420

BNA:	106375687 106376935 106391109 106404715 106420608 106437836 106438521 106447216 106447291 125600622

BOE:	106332194 106337691 106339721 106339957 106341495

RSZ:	108831986 108833239 108834032 108843386 108846044 108849394 108852032

THJ:	104798589 104812935 104814345

CPAP:

110807607 110807640

CIT:	102617333 102617628

CIC:	CICLE_v10016492mg CICLE_v10016504mg

PVY:	116123330 116125778 116125780

MINC:

123205280 123218441 123219609 123227092

TCC:	18595060 18595182

GRA:	105771930 105774077 105793938

GHI:	107910608 107934633 107951058 107963469 121230647

GAB:	108454494 108464796 108484911

DZI:	111275377 111275410 111278543 111280692 111280881

EGR:	104441569 120293137

GMX:	100783704 100784159 100801061 100820263

GSJ:	114388549 114412639 114421452 114425916

PVU:	PHAVU_002G198700g PHAVU_006G125900g

VRA:	106752833 106776020

VAR:	108319376 108343157

VUN:	114176490 114187828

CCAJ:

109811457 109813232

APRC:

113846433 113859182

MTR:	11426615 25493367 25493368

TPRA:

123899350 123915784

CAM:	101501399 101504844

PSAT:

127075055 127108509

LJA:	Lj4g3v0287300.1(Lj4g3v0287300.1) Lj4g3v0299420.1(Lj4g3v0299420.1) Lj6g3v1629500.1(Lj6g3v1629500.1)

ADU:	107481687 107483251

AIP:	107631542 107638868

AHF:	112734575 112741490 112792363 112796199

LANG:

109334910 109350875 109361539

PCIN:

129285016 129304940

FVE:	101298293 101298869 101299165

RCN:	112168406 112201413 112202223 112203169

PPER:

18779884

PMUM:

103325274

PAVI:

110760245

PDUL:

117625755

MDM:	103412368 103446091 103446158 114825261

MSYL:

126586776 126624251 126624427

PXB:	103945064 103952351

ZJU:	107417843 107417928 107417963

MNT:	112091998 21408654

CSAV:

115697693 115698325

CSV:	101203123 101223157

CMO:	103484299 103484300

BHJ:	120072770 120073919

MCHA:

111010918 111015759 111015844

CMAX:

111477811 111490467 111495292 111497491

CMOS:

111433035 111433515 111433982 111457304

CPEP:

111782111 111782888 111802762 111804121

RCU:

8265502

JCU:	105637128 105637129

HBR:	110634570 110642768

MESC:

110616761 110630780

POP:	18103502 7471682

PEU:	105112123 105117604 105134857 105135893

PALZ:

118029496 118035892

JRE:

108990500

CILL:

122277783

QSU:	111987567 112004956

QLO:

115992598

TWL:	119980845 119981207

VVI:	100260984 100266188

VRI:	117923704 117924214

SLY:	101256740 101265381

SPEN:

107010535 107011804

SOT:	102595241 102598435 102601993

SSTN:

125848172 125848529

CANN:

107859057

NTA:	107768658 107779311 107813798 107813799 107821862 107821872

NSY:	104224849 104226041 104250151

NTO:	104113581 104113583 104114399

NAU:	109208707 109239119 109239121

INI:	109153311 109165352 109165353 109174013

ITR:	116026547 116031437 116032268 116032687

SIND:

105167247 105175803

OEU:	111370466 111386603 111401843 111412353

EGT:	105948703 105948863 105951321

SSPL:

121767526 121772093 121775502 121777735 121806884

SMIL:

130985101 130985103

APAN:

127247970 127249517 127254875

HAN:

110911750

ECAD:

122594056

LSV:

111880637

CCAV:

112504751

DCR:	108204907 108205821

CSIN:

114258466 114277079 114302128

RVL:	131312639 131312963 131312964

BVG:	104901225 104901339

SOE:	110775988 110798277

CQI:	110683256 110686561 110716774 110729245 110738420 110739401

MOF:	131159713 131160312

NNU:

104590275

MING:

122087440

TSS:	122640977 122652331

PSOM:

113287276 113294726

OSA:	4334311 4352850

DOSA:

Os03t0780800-01(Os03g0780800) Os12t0628400-01(Os12g0628400)

OBR:	102707731 102713939 102720863

OGL:	127756573 127766230

BDI:	100828932 100834351

ATS:	109744342 109753020

TDC:	119298832 119300335 119303380 119306318 119307094

TAES:

123102026 123105258 123110143 123113515 123119162 123123046

TUA:	125507047 125510800

LPER:

127292579 127298484

LRD:	124652930 124653622 124680474

SBI:	8071505 8083935

ZMA:	100191255 100282761

SITA:

101761592 101780654

SVS:	117836179 117851021

PVIR:

120649563 120667034 120689881 120700208

PHAI:

112874591 112886326

PDA:	103705423 103709837 103719628 103720545 120103882

EGU:	105033213 105046763 105049049 105059835

MUS:	103971669 103975843 103994499

ZOF:	121972439 121978281 121981797 121985651 122010109 122016116

DCT:	110092809 110098017 110100827 110103415

PEQ:	110029131 110032322 110036917

AOF:

109849778

NCOL:

116255392

ATR:

18443053

SMO:	SELMODRAFT_104830 SELMODRAFT_125528

PPP:	112279755 112283141 112289945 112291061 112294897

CRE:	CHLRE_12g496650v5

VCN:	VOLCADRAFT_79982

MNG:	MNEG_15037

CSL:	COCSUDRAFT_39420

APRO:

F751_1031

MIS:	MICPUN_109332

TTC:	FOKN1_1276

SULF:

CAP31_09610

SNIV:

SFSGTM_20430

TMZ:	Tmz1t_1902

THU:	AC731_002855

BID:

Bind_0449

MSL:

Msil_3036

MLG:	CWB41_03150

BBAR:

RHAL1_00524

MSC:

BN69_0792

MBRY:

B1812_10560

MROS:

EHO51_09970

MHEY:

H2LOC_006050

MPAR:

F7D14_02785

MIWA:

SS37A_34520

MTW:	CQW49_11825

RMM:	ROSMUCSMR3_00107

ROK:	RAK1035_2958

NRE:	BES08_12555

NOV:	TQ38_029430

ERK:	CD351_04395

GOX:

GOX1481

GAL:	A0U94_12090

KEU:	S101446_02053

KSC:	CD178_00895

KBA:	A0U89_04215

NCH:	A0U93_02485

BOB:	GN304_04320

BOMB:

GT348_01475

LCK:	HN018_08965

KHA:	IFJ82_00960

SUR:	STAUR_8071

AGE:	AA314_01304

AVM:	JQX13_18815

CFUS:

CYFUS_000746

VIN:	AKJ08_3295

SCL:

sce3951

SCU:	SCE1572_23700

CCRO:

CMC5_051680

MRM:	A7982_03663

LLU:	AKJ09_05212

SCX:	AS200_14350

SGX:	H4W23_13070

BSOL:

FSW04_23010

CWO:

Cwoe_5323

ATM:

ANT_14920

NKO:	Niako_1286

PSEG:

D3H65_09920

PHE:

Phep_1688

SMIZ:

4412673_00846

SPHZ:

E3D81_09710

SPHE:

GFH32_14300

SDJ:	NCTC13534_00990

RHOZ:

GXP67_15205

MTT:	Ftrac_3310

FTE:	Fluta_1455

FLU:	CHH17_16660

» show all

Reference

1 [PMID:19054355]

Authors

Nagano M, Ihara-Ohori Y, Imai H, Inada N, Fujimoto M, Tsutsumi N, Uchimiya H, Kawai-Yamada M

Title

Functional association of cell death suppressor, Arabidopsis Bax inhibitor-1, with fatty acid 2-hydroxylation through cytochrome b(5).

Journal

Plant J 58:122-34 (2009)
DOI:10.1111/j.1365-313X.2008.03765.x

Sequence

[ath:AT2G34770 AT4G20870]

Reference

2 [PMID:22635113]

Authors

Nagano M, Takahara K, Fujimoto M, Tsutsumi N, Uchimiya H, Kawai-Yamada M

Title

Arabidopsis sphingolipid fatty acid 2-hydroxylases (AtFAH1 and AtFAH2) are functionally differentiated in fatty acid 2-hydroxylation and stress responses.

Journal

Plant Physiol 159:1138-48 (2012)
DOI:10.1104/pp.112.199547

Sequence

[ath:AT2G34770 AT4G20870]

Reference

3 [PMID:22918503]

Authors

Nagano M, Uchimiya H, Kawai-Yamada M

Title

Plant sphingolipid fatty acid 2-hydroxylases have unique characters unlike their animal and fungus counterparts.

Journal

Plant Signal Behav 7:1388-92 (2012)
DOI:10.4161/psb.21825