ENZYME: 220.127.116.11 Help
(S)-N-methylcoclaurine oxidase (C-O phenol-coupling)
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
(S)-N-methylcoclaurine,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (C-O phenol-coupling)
(S)-N-methylcoclaurine + (R)-N-methylcoclaurine + [reduced NADPH---hemoprotein reductase] + O2 = berbamunine + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN:
[reduced NADPH---hemoprotein reductase] [CPD:
[oxidized NADPH---hemoprotein reductase] [CPD:
A cytochrome P-450 (heme-thiolate) protein found in plants. Forms the bisbenzylisoquinoline alkaloid berbamunine by phenol oxidation of N-methylcoclaurine without the incorporation of oxygen into the product. Reaction of two molecules of (R)-N-methylcoclaurine gives the dimer guattagaumerine.
EC 18.104.22.168 created 1999 as EC 22.214.171.124, transferred 2002 to EC 126.96.36.199, transferred 2018 to EC 188.8.131.52
ec00950 Isoquinoline alkaloid biosynthesis
ec01110 Biosynthesis of secondary metabolites
Stadler R, Zenk MH.
The purification and characterization of a unique cytochrome P-450 enzyme from Berberis stolonifera plant cell cultures.
J Biol Chem 268:823-31 (1993)
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