KEGG   ENZYME: 1.14.99.39
Entry
EC 1.14.99.39               Enzyme                                 
Name
ammonia monooxygenase;
AMO
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
Miscellaneous
Sysname
ammonia,donor:oxygen oxidoreductase (hydroxylamine-producing)
Reaction(IUBMB)
NH3 + a reduced acceptor + O2 = NH2OH + an acceptor + H2O [RN:R09519]
Reaction(KEGG)
R09519 > R00148;
(other) R09156
Substrate
NH3 [CPD:C00014];
reduced acceptor [CPD:C00030];
O2 [CPD:C00007]
Product
NH2OH [CPD:C00192];
acceptor [CPD:C00028];
H2O [CPD:C00001]
Comment
The enzyme catalyses the first reaction in the pathway of ammonia oxidation to nitrite. It contains copper [1], iron [5] and possibly zinc [9]. The enzyme requires two electrons, which are derived indirectly from the quinone pool via a membrane-bound donor.
History
EC 1.14.99.39 created 2010
Pathway
ec00910  Nitrogen metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
ec01310  Nitrogen cycle
Orthology
K28504  ammonia monooxygenase subunit A
Genes
NEU: NE0944(amoA1) NE2063(amoA2)
NET: Neut_2077 Neut_2318
NIT: NAL212_0798 NAL212_1387 NAL212_2605
NII: Nit79A3_0472 Nit79A3_1080 Nit79A3_2885
NCO: AAW31_01085 AAW31_05380
NUR: ATY38_01310 ATY38_07255 ATY38_13755
NST: Nstercoris_00328 Nstercoris_01426
NMU: Nmul_A0799 Nmul_A2325 Nmul_A2765
NLC: EBAPG3_006230 EBAPG3_014830 EBAPG3_15005
NIZ: NNRS527_00820 NNRS527_02502 NNRS527_03102
AZR: CJ010_10955
NIO: NITINOP_1853(amoA)
NKF: Nkreftii_003170
NMR: Nmar_1500
NIR: NSED_08255
NKR: NKOR_08170
NID: NPIRD3C_2147(amoA)
NIN: NADRNF5_0394(amoA)
NIW: Nisw_06415
NCL: C5F47_07870
NOX: C5F49_07755
NUE: C5F50_11085
NIP: NsoK4_00795
NZT: NZOSNM25_000290
NCT: NMSP_0259
NIC: DSQ20_08765
NGA: Ngar_c25350(amoA)
NVN: NVIE_027270(amoA)
NEV: NTE_00961
TAA: NMY3_03280
NFN: NFRAN_0675(amoA)
NCV: NCAV_0492(amoA)
NDV: NDEV_1777(amoA)
CSY: CENSYa_0402
NBV: T478_0302
TAH: SU86_008325
NIU: DSQ19_08460
NCK: QVH35_01005
 » show all
Reference
1  [PMID:8458839]
  Authors
Ensign SA, Hyman MR, Arp DJ
  Title
In vitro activation of ammonia monooxygenase from Nitrosomonas europaea by copper.
  Journal
J Bacteriol 175:1971-80 (1993)
DOI:10.1128/JB.175.7.1971-1980.1993
Reference
2  [PMID:8085841]
  Authors
Hyman MR, Page CL, Arp DJ
  Title
Oxidation of methyl fluoride and dimethyl ether by ammonia monooxygenase in Nitrosomonas europaea.
  Journal
Appl Environ Microbiol 60:3033-5 (1994)
DOI:10.1128/AEM.60.8.3033-3035.1994
Reference
3  [PMID:7980540]
  Authors
Bergmann DJ, Hooper AB
  Title
Sequence of the gene, amoB, for the 43-kDa polypeptide of ammonia monoxygenase of Nitrosomonas europaea.
  Journal
Biochem Biophys Res Commun 204:759-62 (1994)
DOI:10.1006/bbrc.1994.2524
  Sequence
[neu:NE0944 NE0943]
Reference
4  [PMID:7590173]
  Authors
Holmes AJ, Costello A, Lidstrom ME, Murrell JC
  Title
Evidence that particulate methane monooxygenase and ammonia monooxygenase may be evolutionarily related.
  Journal
FEMS Microbiol Lett 132:203-8 (1995)
DOI:10.1111/j.1574-6968.1995.tb07834.x
Reference
5  [PMID:8941709]
  Authors
Zahn JA, Arciero DM, Hooper AB, DiSpirito AA
  Title
Evidence for an iron center in the ammonia monooxygenase from Nitrosomonas europaea.
  Journal
FEBS Lett 397:35-8 (1996)
DOI:10.1016/S0014-5793(96)01116-7
Reference
6  [PMID:8654570]
  Authors
Moir JW, Crossman LC, Spiro S, Richardson DJ
  Title
The purification of ammonia monooxygenase from Paracoccus denitrificans.
  Journal
FEBS Lett 387:71-4 (1996)
DOI:10.1016/0014-5793(96)00463-2
Reference
7  [PMID:11004450]
  Authors
Whittaker M, Bergmann D, Arciero D, Hooper AB
  Title
Electron transfer during the oxidation of ammonia by the chemolithotrophic bacterium Nitrosomonas europaea.
  Journal
Biochim Biophys Acta 1459:346-55 (2000)
DOI:10.1016/S0005-2728(00)00171-7
Reference
8  [PMID:12209257]
  Authors
Arp DJ, Sayavedra-Soto LA, Hommes NG.
  Title
Molecular biology and biochemistry of ammonia oxidation by Nitrosomonas europaea.
  Journal
Arch Microbiol 178:250-5 (2002)
DOI:10.1007/s00203-002-0452-0
Reference
9  [PMID:19453274]
  Authors
Gilch S, Meyer O, Schmidt I
  Title
A soluble form of ammonia monooxygenase in Nitrosomonas europaea.
  Journal
Biol Chem 390:863-73 (2009)
DOI:10.1515/BC.2009.085
Other DBs
ExplorEnz - The Enzyme Database: 1.14.99.39
IUBMB Enzyme Nomenclature: 1.14.99.39
ExPASy - ENZYME nomenclature database: 1.14.99.39
UM-BBD (Biocatalysis/Biodegradation Database): 1.14.99.39
BRENDA, the Enzyme Database: 1.14.99.39
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