EC 126.96.36.199 Enzyme
pyruvate dehydrogenase system;
pyruvate dehydrogenase complex;
Acting on the aldehyde or oxo group of donors;
With NAD+ or NADP+ as acceptor
pyruvate:NAD+ 2-oxidoreductase (CoA-acetylating)
pyruvate + CoA + NAD+ = acetyl-CoA + CO2 + NADH [RN:
The pyruvate dehydrogenase system (PDH) is a large enzyme complex that belongs to the 2-oxoacid dehydrogenase system family, which also includes EC
, branched-chain alpha-keto acid dehydrogenase system, EC
, 2-oxoglutarate dehydrogenase system, EC
, glycine cleavage system, and EC
, acetoin dehydrogenase system. With the exception of the glycine cleavage system, which contains 4 components, the 2-oxoacid dehydrogenase systems share a common structure, consisting of three main components, namely a 2-oxoacid dehydrogenase (E1), a dihydrolipoamide acyltransferase (E2), and a dihydrolipoamide dehydrogenase (E3). The reaction catalysed by this system is the sum of three activities: EC
, pyruvate dehydrogenase (acetyl-transferring) (E1), EC
, dihydrolipoyllysine-residue acetyltransferase (E2), and EC
, dihydrolipoyl dehydrogenase (E3). The mammalian system also includes E3 binding protein, which is involved in the interaction between the E2 and E3 subunits.
EC 188.8.131.52 created 2020
Reed LJ, Pettit FH, Eley MH, Hamilton L, Collins JH, Oliver RM.
Reconstitution of the Escherichia coli pyruvate dehydrogenase complex.
Proc Natl Acad Sci U S A 72:3068-72 (1975)
Bates DL, Danson MJ, Hale G, Hooper EA, Perham RN.
Self-assembly and catalytic activity of the pyruvate dehydrogenase multienzyme complex of Escherichia coli.
Nature 268:313-6 (1977)
Stanley CJ, Packman LC, Danson MJ, Henderson CE, Perham RN.
Intramolecular coupling of active sites in the pyruvate dehydrogenase multienzyme complexes from bacterial and mammalian sources.
Biochem J 195:715-21 (1981)
Yang HC, Hainfeld JF, Wall JS, Frey PA.
Quaternary structure of pyruvate dehydrogenase complex from Escherichia coli.
J Biol Chem 260:16049-51 (1985)
Patel MS, Roche TE.
Molecular biology and biochemistry of pyruvate dehydrogenase complexes.
FASEB J 4:3224-33 (1990)
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