KEGG ENZYME: 1.2.7.7

ENZYME: 1.2.7.7

Entry

EC 1.2.7.7                  Enzyme

Name

3-methyl-2-oxobutanoate dehydrogenase (ferredoxin);
2-ketoisovalerate ferredoxin reductase;
3-methyl-2-oxobutanoate synthase (ferredoxin);
VOR;
branched-chain ketoacid ferredoxin reductase;
branched-chain oxo acid ferredoxin reductase;
keto-valine-ferredoxin oxidoreductase;
ketoisovalerate ferredoxin reductase;
2-oxoisovalerate ferredoxin reductase

Class

Oxidoreductases;
Acting on the aldehyde or oxo group of donors;
With an iron-sulfur protein as acceptor

Sysname

3-methyl-2-oxobutanoate:ferredoxin oxidoreductase (decarboxylating; CoA-2-methylpropanoylating)

Reaction(IUBMB)

3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO2 + 2 reduced ferredoxin + H+ [RN:R07160]

Reaction(KEGG)

R07160;
(other) R08566 R08567

Substrate

3-methyl-2-oxobutanoate [CPD:C00141];
CoA [CPD:C00010];
oxidized ferredoxin [CPD:C00139]

Product

S-(2-methylpropanoyl)-CoA;
CO2 [CPD:C00011];
reduced ferredoxin [CPD:C00138];
H+ [CPD:C00080]

Comment

The enzyme is CoA-dependent and contains thiamine diphosphate and iron-sulfur clusters. Preferentially utilizes 2-oxo-acid derivatives of branched chain amino acids, e.g. 3-methyl-2-oxopentanoate, 4-methyl-2-oxo-pentanoate, and 2-oxobutanoate. This enzyme is a member of the 2-oxoacid oxidoreductases, a family of enzymes that reversibly catalyse the oxidative decarboxylation of different 2-oxoacids to form their CoA derivatives, and are differentiated based on their substrate specificity. For examples of other members of this family, see EC 1.2.7.1, pyruvate synthase, and EC 1.2.7.3, 2-oxoglutarate synthase.

History

EC 1.2.7.7 created 2003

Pathway

ec00280

Valine, leucine and isoleucine degradation

ec01100

Metabolic pathways

Orthology

K00186

2-oxoisovalerate ferredoxin oxidoreductase alpha subunit

K00187

2-oxoisovalerate ferredoxin oxidoreductase beta subunit

K00188

2-oxoisovalerate ferredoxin oxidoreductase delta subunit

K00189

2-oxoisovalerate/pyruvate ferredoxin oxidoreductase gamma subunit

Genes

HMR:	Hipma_0796 Hipma_0797 Hipma_0798

DAV:	DESACE_01535 DESACE_01540(porA) DESACE_01545

PEF:	A7E78_02850 A7E78_02855

DBK:	DGMP_23530

DALK:

DSCA_29970

DLI:	dnl_19240(porB)

DMM:	dnm_023500

DEK:	DSLASN_10380 DSLASN_10400(porA_1)

DAW:	HS1_001571(porC)

AFW:	Anae109_4113 Anae109_4114

AORY:

AMOR_34150 AMOR_34170(porA)

DHR:	LGS26_04510

PFAA:

MM59RIKEN_15740

FWA:	DCMF_13980

EVN:	NQ558_06000

CHYL:

CE91St63_20520

HPRF:

HLPR_01110(porD)

CALK:

HUE98_02340 HUE98_02350(porA)

MPAG:

C0J29_14490

LMAJ:

GKN94_01220

PBU:	L21SP3_01136 L21SP3_01137(korA_1) L21SP3_01138(korB_1)

PBP:	STSP1_01191 STSP1_01192(korA_1) STSP1_01193(korB_1)

PBAS:

SMSP2_01215(korB) SMSP2_01216(korA) SMSP2_01217

VBC:	C5Q97_08000 C5Q97_08005 C5Q97_08010

SLR:	L21SP2_0405 L21SP2_0406 L21SP2_0407

ABA:	Acid345_0952 Acid345_0953

ORP:	MOP44_03550(vorB) MOP44_03555

SUS:	Acid_1870 Acid_1871

PFER:

IRI77_33000 IRI77_33005

THYD:

TTHT_1225(porA) TTHT_1226(porB)

GEX:	GETHOR_18790 GETHOR_18800

GEP:	Q9293_11035(vorB) Q9293_11040

GET:	QOZ81_002117

MSIL:

METEAL_31510

MSEA:

METESE_27950

DUQ:	E7745_05455

CABY:

Cabys_3126 Cabys_3127

FBN:	HY931_02725

MMP:	MMP1271(vorA) MMP1272(vorB) MMP1273(vorC)

MMQ:	MmarC5_0318 MmarC5_0319 MmarC5_0320

MMX:	MmarC6_1400 MmarC6_1401 MmarC6_1402

MMZ:	MmarC7_0517 MmarC7_0518 MmarC7_0519

MMD:	GYY_07190 GYY_07195 GYY_07200

MMAK:

MMKA1_14960 MMKA1_14970 MMKA1_14980

MMAO:

MMOS7_13780 MMOS7_13790 MMOS7_13800

MMAD:

MMJJ_15720 MMJJ_15730(korA_3) MMJJ_15740(korB_3)

MVN:	Mevan_0583 Mevan_0584 Mevan_0585

MVO:	Mvol_0718 Mvol_0719 Mvol_0720

MTH:	MTH_703 MTH_704 MTH_705

MMG:	MTBMA_c10900(vorC) MTBMA_c10910(vorB) MTBMA_c10920(vorA1) MTBMA_c10930(vorA2)

MWO:	MWSIV6_1079(vorC) MWSIV6_1080(vorB) MWSIV6_1081(vorA)

MTEE:

MTTB_10540 MTTB_10550 MTTB_10560

METC:

MTCT_0631 MTCT_0632 MTCT_0633

METE:

tca_00678 tca_00679(korA_2) tca_00680(korB_2)

METZ:

METMT2_0337 METMT2_0338 METMT2_0339

METK:

FVF72_08025 FVF72_08030 FVF72_08035

MTHM:

FZP57_05120 FZP57_05125 FZP57_05130

METJ:

FZP68_01095 FZP68_01100 FZP68_01105

MRU:	mru_1431(vorB) mru_1432(vorA) mru_1433(vorC)

MSI:	Msm_0331 Msm_0332 Msm_0333

MMIL:

sm9_0692(vorB) sm9_0693(vorA) sm9_0694(vorC)

MEYE:

TL18_03385

MOL:	YLM1_0932 YLM1_0933 YLM1_0934

MARO:

MarbSA_03660 MarbSA_03670 MarbSA_03680

METV:

K4897_02170 K4897_02175 K4897_02180

MEL:	Metbo_2452 Metbo_2453 Metbo_2454

MEW:	MSWAN_0187 MSWAN_0188 MSWAN_0189

METH:

MBMB1_0178 MBMB1_0179(vorB) MBMB1_0180(vorA)

MFC:	BRM9_2123(vorB) BRM9_2124(vorA) BRM9_2125(vorC)

MFI:	DSM1535_1555 DSM1535_1556(vorB) DSM1535_1557(vorA)

MCUB:

MCBB_0193(vorC) MCBB_0194(vorB) MCBB_0195(vorA)

MSUB:

BK009_10645 BK009_10650 BK009_10655

METN:

BK008_06270 BK008_06275 BK008_06280

METT:

CIT01_01270 CIT01_01275 CIT01_01280

METO:

CIT02_06140 CIT02_06145 CIT02_06150

MEME:

HYG87_02760 HYG87_02765 HYG87_02770

MFEG:

GCM10025860_09900 GCM10025860_09920 GCM10025860_09930

MFV:	Mfer_0416 Mfer_0417 Mfer_0506

AST:	Asulf_00880

GAC:

GACE_2154

GAH:

GAH_00571

PFU:	PF0968 PF0969 PF0970 PF0971

PFI:	PFC_04070 PFC_04075(vorA) PFC_04080(vorD) PFC_04085

PHO:	PH0678(PH0678) PH0679(PH0679) PH0680(PH0680) PH0681(PH0681)

PAB:	PAB1470 PAB1471 PAB1472 PAB1473

PYN:	PNA2_1292 PNA2_1293 PNA2_1294 PNA2_1295

PYA:	PYCH_06630 PYCH_06640 PYCH_06650 PYCH_06660

PYS:	Py04_1079 Py04_1080 Py04_1081 Py04_1082

PYC:	TQ32_04845 TQ32_04850(vorD) TQ32_04855(porA) TQ32_04860

TKO:	TK1978 TK1979 TK1980 TK1981

TON:	TON_1476 TON_1477 TON_1478 TON_1479

TGA:	TGAM_0264(vorB) TGAM_0265(vorA) TGAM_0266(vorD) TGAM_0267(porG)

TSI:	TSIB_1381 TSIB_1382 TSIB_1383 TSIB_1384

TBA:	TERMP_01557 TERMP_01558 TERMP_01559 TERMP_01560

THE:	GQS_08125 GQS_08130(vorA) GQS_08135(vorD) GQS_08140

THA:	TAM4_1107 TAM4_1398 TAM4_1406 TAM4_1460

THM:	CL1_1246 CL1_1247 CL1_1248 CL1_1249

TLT:	OCC_07486 OCC_07491(porA) OCC_07496(vorD) OCC_07501

THS:	TES1_1552 TES1_1553 TES1_1554 TES1_1555

TNU:	BD01_1941 BD01_1942 BD01_1943 BD01_1944

TEU:	TEU_01670 TEU_01675(porA) TEU_01680(vorD) TEU_01685

TGY:	X802_03760 X802_03765(porA) X802_03770(vorD) X802_03775

THV:	ADU37_CDS14440 ADU37_CDS14450 ADU37_CDS14460 ADU37_CDS14470

TCH:	CHITON_0665 CHITON_0666 CHITON_0667 CHITON_0668

TPEP:

A0127_04995 A0127_05000(vorD) A0127_05005(porA) A0127_05010

TPIE:

A7C91_04895 A7C91_04900(porA) A7C91_04905(vorD) A7C91_04910

TGG:	A3K92_07955 A3K92_07960(vorD) A3K92_07965(porA) A3K92_07970

TCE:	A3L02_06800 A3L02_06805(porA) A3L02_06810(vorD) A3L02_06815

TBS:	A3L01_08180 A3L01_08185(vorD) A3L01_08190(porA) A3L01_08195

THH:	CDI07_07385 CDI07_07390(porA) CDI07_07395(vorD) CDI07_07400

TSL:	A3L11_01530 A3L11_01535(vorD) A3L11_01540(porA) A3L11_01545

TTD:	A3L14_10575 A3L14_10580(vorD) A3L14_10585(porA) A3L14_10590

TPRF:

A3L09_01305 A3L09_01310(vorD) A3L09_01315(porA) A3L09_01320

TRL:	A3L10_07570 A3L10_07575(vorD) A3L10_07580(porA) A3L10_07585

TPAF:

A3L08_01305 A3L08_01310(vorD) A3L08_01315(porA) A3L08_01320

THY:	A3L12_04015 A3L12_04020(vorD) A3L12_04025(porA) A3L12_04030

TIC:	FH039_11775 FH039_11780 FH039_11785(porA) FH039_11790

TCQ:	TIRI35C_1959(porG) TIRI35C_1960(vorD) TIRI35C_1961(vorA) TIRI35C_1962(vorB)

THEM:

FPV09_09745 FPV09_09750(porA) FPV09_09755 FPV09_09760

THEI:

K1720_10000 K1720_10005(porA) K1720_10010 K1720_10015

PPAC:

PAP_04300 PAP_04305(vorD) PAP_04310(porA) PAP_04315

MBA:	Mbar_A2173 Mbar_A2174 Mbar_A2175

MBY:	MSBRM_2262 MSBRM_2263 MSBRM_2264

MBW:	MSBRW_2566 MSBRW_2567 MSBRW_2568

MBAR:

MSBR2_0147 MSBR2_0148 MSBR2_0149

MBAK:

MSBR3_1064 MSBR3_1065 MSBR3_1066

MAC:	MA_2909(vorA) MA_2910(vorB) MA_2911(vorC)

MMA:	MM_3181(vorC) MM_3182(vorB) MM_3183(vorA)

MMAZ:

MmTuc01_3277 MmTuc01_3278 MmTuc01_3279

MMJ:	MSMAS_1506 MSMAS_1507 MSMAS_1508

MMAC:

MSMAC_2849 MSMAC_2850 MSMAC_2851

MVC:	MSVAZ_1286 MSVAZ_1287 MSVAZ_1288

MEK:	MSKOL_1230 MSKOL_1231 MSKOL_1232

MLS:	MSLAZ_2008 MSLAZ_2009 MSLAZ_2010

METM:

MSMTP_1761 MSMTP_1762 MSMTP_1763

MEF:	MSWH1_1307 MSWH1_1308 MSWH1_1309

MEQ:	MSWHS_1391 MSWHS_1392 MSWHS_1393

MSJ:	MSSAC_2721 MSSAC_2722 MSSAC_2723

MSZ:	MSSIH_1391 MSSIH_1392 MSSIH_1393

MSW:	MSSIT_1429 MSSIT_1430 MSSIT_1431

MTHR:

MSTHT_0824 MSTHT_0825 MSTHT_0826

MTHE:

MSTHC_2445 MSTHC_2446 MSTHC_2447

MHOR:

MSHOH_1579 MSHOH_1580 MSHOH_1581

MFZ:	AOB57_012375 AOB57_012380(vorB) AOB57_012385

MBU:	Mbur_0605 Mbur_0606 Mbur_0607

MMET:

MCMEM_1077 MCMEM_1078 MCMEM_1079

MELO:

J7W08_03845 J7W08_03850 J7W08_03855

MMH:	Mmah_1010 Mmah_1011 Mmah_1012

MHAZ:

BHR79_08510 BHR79_08515 BHR79_08520

MPOT:

BKM01_04760 BKM01_04765 BKM01_04770

MZH:	Mzhil_0902 Mzhil_0903 Mzhil_0904

MPY:	Mpsy_0059 Mpsy_0060 Mpsy_0061

MZI:	HWN40_07260 HWN40_07265 HWN40_07270

MMAV:

RE476_03225 RE476_03230 RE476_03235

MSEB:

RE474_12875 RE474_12880 RE474_12885

MHZ:	Metho_2048 Metho_2049 Metho_2050

MEHF:

MmiHf6_00120 MmiHf6_00130(korA) MmiHf6_00140

MEES:

MmiEs2_10520 MmiEs2_10530(korA) MmiEs2_10540

MTP:	Mthe_0156 Mthe_0157

MCJ:	MCON_0778(vorA) MCON_0779(vorB)

MHI:	Mhar_0583 Mhar_0584

MAQE:

RJ40_07300

MFK:	E2N92_07470

HAHS:

HSRCO_0562(porG) HSRCO_0563(porA2)

HAAA:

AArcCO_0208(porD)

TAC:	Ta0626 Ta0627 Ta0628 Ta0629

TVO:	TVG0872869(TVG0872869)

MER:	MMINT_12450(vorD) MMINT_12470

MEAR:

Mpt1_c05540(vorA) Mpt1_c05550(vorB)

ABI:	Aboo_0298 Aboo_0299 Aboo_0300 Aboo_0301

ACF:	AciM339_0763 AciM339_0764 AciM339_0765 AciM339_0766

IPC:

IPA_03975

IAG:

Igag_1136

PABI:

PABY_00170

PARE:

PYJP_01300

STO:

STK_15320

SOH:	D1869_08595

SSO:	SSO1207(porA-1)

SOL:

Ssol_2171

SSOA:

SULA_2206

SSOL:

SULB_2207

SSOF:

SULC_2204

SSHI:

J5U23_01993

SIS:	LS215_0396

SIA:	M1425_0372

SIM:	M1627_0380

SID:

M164_0396

SIY:	YG5714_0374

SIN:	YN1551_2681

SII:

LD85_0384

SIH:

SiH_0373

SIR:

SiRe_0364

SIC:

SiL_0352

SULE:

GFS03_03425

SULO:

GFS33_07805

SULL:

EWF20_09765

MTEN:

GWK48_07885

AMAN:

B6F84_13225

ASUL:

DFR86_11210

SMET:

RQ359_000910

CCAI:

NAS2_0099 NAS2_0791

FLT:	Sv326_0312

LOB:	NEF87_002289 NEF87_002291 NEF87_002419

PSYT:

DSAG12_00253 DSAG12_00810

AGW:	QT03_C0001G0327

» show all

Reference

1 [PMID:5800441]

Authors

Buchanan BB.

Title

Role of ferredoxin in the synthesis of alpha-ketobutyrate from propionyl coenzyme A and carbon dioxide by enzymes from photosynthetic and nonphotosynthetic bacteria.

Journal

J Biol Chem 244:4218-23 (1969)

Reference

2 [PMID:8550513]

Authors

Heider J, Mai X, Adams MW

Title

Characterization of 2-ketoisovalerate ferredoxin oxidoreductase, a new and reversible coenzyme A-dependent enzyme involved in peptide fermentation by hyperthermophilic archaea.

Journal

J Bacteriol 178:780-7 (1996)
DOI:10.1128/JB.178.3.780-787.1996

Reference

3 [PMID:9108258]

Authors

Tersteegen A, Linder D, Thauer RK, Hedderich R

Title

Structures and functions of four anabolic 2-oxoacid oxidoreductases in Methanobacterium thermoautotrophicum.

Journal

Eur J Biochem 244:862-8 (1997)
DOI:10.1111/j.1432-1033.1997.00862.x

Reference

4 [PMID:11265457]

Authors

Schut GJ, Menon AL, Adams MW.

Title

2-keto acid oxidoreductases from Pyrococcus furiosus and Thermococcus litoralis.

Journal

Methods Enzymol 331:144-58 (2001)
DOI:10.1016/S0076-6879(01)31053-4

Other DBs

ExplorEnz - The Enzyme Database:

1.2.7.7

IUBMB Enzyme Nomenclature:

1.2.7.7

ExPASy - ENZYME nomenclature database:

1.2.7.7

BRENDA, the Enzyme Database:

1.2.7.7

LinkDB

DBGET integrated database retrieval system