The enzyme contains a tightly but noncovalently bound NADP(H) cofactor, as well as Zn2+ and Mg2+. Enzyme-bound NADPH formed by oxidation of formaldehyde to formate is oxidized back to NADP+ by reaction with a second formaldehyde, yielding methanol. The enzyme from the bacterium Mycobacterium sp. DSM 3803 also catalyses the reactions of EC 188.8.131.52, alcohol dehydrogenase (nicotinoprotein) and EC 184.108.40.206, methanol dehydrogenase (nicotinoprotein) . Formaldehyde and acetaldehyde can act as donors; formaldehyde, acetaldehyde and propanal can act as acceptors [1,2].
EC 220.127.116.11 created 1986 as EC 18.104.22.168, modified 2012, transferred 2015 to EC 22.214.171.124