KEGG ENZYME: 1.21.3.8

ENZYME: 1.21.3.8

Entry

EC 1.21.3.8                 Enzyme

Name

cannabidiolic acid synthase;
CBDA synthase

Class

Oxidoreductases;
Catalysing the reaction X-H + Y-H = X-Y;
With oxygen as acceptor

Sysname

cannabigerolate:oxygen oxidoreductase (cyclizing, cannabidiolate-forming)

Reaction(IUBMB)

cannabigerolate + O2 = cannabidiolate + H2O2 [RN:R10160]

Reaction(KEGG)

R10160

Substrate

cannabigerolate [CPD:C20406];
O2 [CPD:C00007]

Product

cannabidiolate [CPD:C10784];
H2O2 [CPD:C00027]

Comment

Binds FAD covalently. Part of the cannabinoids biosynthetic pathway of the plant Cannabis sativa. The enzyme can also convert cannabinerolate to cannabidiolate with lower efficiency.

History

EC 1.21.3.8 created 2012

Pathway

ec00999

Biosynthesis of various plant secondary metabolites

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K20502

cannabidiolic acid synthase

Genes

CSAV:

115697762

Reference

1 [PMID:8663284]

Authors

Taura F, Morimoto S, Shoyama Y

Title

Purification and characterization of cannabidiolic-acid synthase from Cannabis sativa L.. Biochemical analysis of a novel enzyme that catalyzes the oxidocyclization of cannabigerolic acid to cannabidiolic acid.

Journal

J Biol Chem 271:17411-6 (1996)
DOI:10.1074/jbc.271.29.17411

Sequence

[ag:BAF65033]

Reference

2 [PMID:17544411]

Authors

Taura F, Sirikantaramas S, Shoyama Y, Yoshikai K, Shoyama Y, Morimoto S

Title

Cannabidiolic-acid synthase, the chemotype-determining enzyme in the fiber-type Cannabis sativa.

Journal

FEBS Lett 581:2929-34 (2007)
DOI:10.1016/j.febslet.2007.05.043