KEGG   ENZYME: 1.3.1.85
Entry
EC 1.3.1.85                 Enzyme                                 
Name
crotonyl-CoA carboxylase/reductase;
CCR;
crotonyl-CoA reductase (carboxylating)
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
(2S)-ethylmalonyl-CoA:NADP+ oxidoreductase (decarboxylating)
Reaction(IUBMB)
(2S)-ethylmalonyl-CoA + NADP+ = (E)-but-2-enoyl-CoA + CO2 + NADPH + H+ [RN:R09291]
Reaction(KEGG)
R09291
Substrate
(2S)-ethylmalonyl-CoA [CPD:C18026];
NADP+ [CPD:C00006]
Product
(E)-but-2-enoyl-CoA [CPD:C00877];
CO2 [CPD:C00011];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
The reaction is catalysed in the reverse direction. This enzyme, isolated from the bacterium Rhodobacter sphaeroides, catalyses (E)-but-2-enoyl-CoA-dependent oxidation of NADPH in the presence of CO2. When CO2 is absent, the enzyme catalyses the reduction of (E)-but-2-enoyl-CoA to butanoyl-CoA, but with only 10% of maximal activity (relative to (E)-but-2-enoyl-CoA carboxylation).
History
EC 1.3.1.85 created 2011
Pathway
ec00630  Glyoxylate and dicarboxylate metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K14446  crotonyl-CoA carboxylase/reductase
Genes
ECAECA0600(cfa8B)
PATREV46_04860
PATOGZ59_09850(cfa8B)
DICDpoa569_002944(ccrA)
BNGEH206_19780(ccrA)
LBQCKQ53_08765(ccrA)
PAYPAU_02213
PSTPSPTO_4689(cfa8)
PAMGBKM19_030840(ccrA)
PCABJGS08_03260(ccrA)
PCOFPOR16_03640(ccrA)
PTREI9H09_26050(ccrA)
PPVNJ69_15265
PIZLAB08_R41860(ccrA)
ALKMNKI27_11405(ccrA)
ASEMNNL22_07760(ccrA)
ZPLZBT109_0360
OMEOLMES_2177
BVEAK36_5943(ccrA)
BAMBamb_6221
BCONNL30_35040
BARINLX30_36090(ccrA)
BGPBGL_1c19630
BPLAbpln_1g17940
PARABTO02_25445
PEWKZJ38_27745(ccrA)
JAJEKL02_10350(ccrA)
APPCAP2UW1_2501
CFUSCYFUS_003175
VINAKJ08_0760
PLAPlav_1621
RBSRHODOSMS8_02646(ccrA2)
BGQX265_36830(ccrA)
BGZXH91_36035(ccrA)
BVZBRAD3257_7532(ccr)
BOPAXW83_03430
BOSBSY19_2139(ccrA)
BVVBHK69_16350
BOIBLM15_10545
BOFFQV39_08895(ccrA)
XAUXaut_1195
XDIEZH22_01060(ccrA)
AZCAZC_0997
MEAMex_1p0178(ccr)
MDIMETDI0161(ccr)
MEXMext_0288
MCHMchl_0332
MPOMpop_0364
MRDMrad2831_1105
METM446_0037 M446_5660
MNOMnod_0039
MORMOC_1328(ccrA)
METAY590_00895
MAQUMaq22A_1p32000(ccrA)
MPHYMCBMB27_00696
MEEDA075_05075(ccrA)
METDC0214_01265(ccrA)
METXA3862_25070
METSDK389_18840(ccrA)
METIDK427_18535(ccrA)
MMESMMSR116_22775(ccrA)
MTEADK419_07965(ccrA)
MINDmvi_52670(ccr)
MOGMMB17_10655(ccrA)
RHJHZY79_04295(ccrA)
CHELAL346_09685
CDQBOQ54_02760
HDNHden_0216
HDTHYPDE_23643
HMCHYPMC_0098(ccr)
HNIW911_17400
RVARvan_2964
FILBN1229_v1_1373(ccr)
FIYBN1229_v1_1372(ccr)
RHZRHPLAN_11780
MCGGL4_3337
METGHT051_03000(ccrA)
BVRBVIR_87
BLAGBLTE_34310
MSCBN69_1948
MBRYB1812_02315
MROSEHO51_03605(ccrA) EHO51_15500(ccrA) EHO51_19170(ccrA)
MHEYH2LOC_016875(ccrA)
MPARF7D14_06270(ccrA)
MTWCQW49_03790(ccrA)
CMETK6K41_13855(ccrA)
PLEOOHA_1_02831(ccrA)
HDIHDIA_3961(ccrA2)
PSINCAK95_11885
NOHG5V57_10405(ccrA)
RBMTEF_03480
PSFPSE_0115
PPHRAPZ00_07795
LAPACP90_02770
LABRCHH27_17725(ccrA)
LABPFJ695_06475(ccrA)
LABTFIU93_08395(ccrA1)
LAGGB0E33_14250
SIWGH266_11590(ccrA)
PHRC6569_14500(ccrA)
PSTGE8M01_09065(ccrA)
CCRCC_3085
CCSCCNA_03181
CAKCaul_1067
CSECseg_3646
CHQAQ619_02890
CMBCSW64_02210(ccrA)
CFHC1707_03615(ccrA)
CAUFCSW63_19590(ccrA)
TSVDSM104635_01214(ccrA2)
SILSPO0370(ccrA)
SITTM1040_3104
RUAD1823_12085(ccrA)
RUTFIU92_01410(ccrA1)
RCONK3740_01540(ccrA)
JANJann_0793
RDERD1_1637(ccrA)
RLIRLO149_c030710(ccr)
RPONG3256_15260(ccrA)
DSHDshi_2853
PGAPGA1_c03870
PGLPGA2_c03420
PGDGal_03115
PHPPhaeoP97_02969
PPICPhaeoP14_00304
PHQD1820_14795(ccrA)
OATOAN307_c41350(ccr)
OAROA238_c04430(ccr)
OTMOSB_04920(ccrA2)
OCTFTO60_13700(ccrA)
LMDMETH_03410
LEJETW24_04250(ccrA)
LAQUR2C4_13535(ccrA)
LEVETW23_15950(ccrA)
LCAEK3721_03535(ccrA)
REDroselon_01708
CIDP73_3852
CMARIMCC12053_3007
CEHCEW89_10025(ccrA)
MALGMALG_02723
CONTQ29_02510
YPACCEW88_11095(ccrA)
YANAYJ57_04475
TPROGa0080559_TMP1106
PABYGa0080574_TMP3170
SUAMBOO69_13585
SULZC1J03_18095(ccrA)
SULIC1J05_18020(ccrA)
SULDB5M07_05055
SPOTG6548_04225(ccrA)
SMEDJNX03_18140(ccrA)
SINLDSM14862_02472(ccrA2_2)
SPSESULPSESMR1_00409(ccrA2)
TOMBWR18_01125
RMMROSMUCSMR3_01832(ccrA2)
ROKRAK1035_1354
RIDRIdsm_00116(ccrA2_1)
ROMEI983_03055(ccrA)
ROHFIU89_18780(ccrA2)
AHTANTHELSMS3_02145(ccr)
SAGUCDO87_04300(ccrA)
THAACFI11_20735(ccrA)
OCDFHY55_05955(ccrA)
MARUFIU81_04185(ccrA2)
ROTFIV09_01485(ccrA2)
RMHLVO79_01145(ccrA)
MALUKU6B_41650(ccr)
TGLHFZ77_08320(ccrA)
TMDKUV46_08220(ccrA)
HATRC74_09425
PALXGQA70_04865(ccrA)
LSALKBK07_01490(ccrA)
RSPRSP_0960
RSHRsph17029_2620
RSQRsph17025_3009
RSKRSKD131_2352
CAZTLV780_12820(ccrA)
RCPRCAP_rcc03243(ccrA)
RHPLPB142_14610
RBLB6K69_07515
PDEPden_3873
PAMIJCM7686_1036
PYEA6J80_12555(ccrA)
PCONB0A89_06930
PZHCX676_11125(ccrA)
PAROCUV01_03100(ccrA)
PAMNJCM7685_1439
PMUTDPM13_11015(ccrA)
PARSDRW48_03195(ccrA)
PARREOJ32_14935(ccrA)
PAAKFIU66_03190(ccrA)
PKDF8A10_08040(ccrA)
PPANESD82_06425(ccrA)
PLIAE4191_03280(ccrA)
PSANHGN31_12030(ccrA)
PMAUCP157_00622(ccrA2)
PMEHJWJ88_03135(ccrA)
PTPRCA23_c09190(ccr)
RSUNHU_00739
RHMB5V46_04550
RHCRGUI_0609
RMBK529_020845
DAAAKL17_0912
DONBSK21_00885
RBZB9057_00670
THWBMG03_18280
TECAKL02_018635(ccrA)
LVSLOKVESSMR4R_02786(ccrA2)
RBGBG454_11885(ccrA)
GEHHYN69_12810(ccrA)
GFUKM031_04390(ccrA)
TAWEI545_06880(ccrA)
SALOEF888_18445(ccrA)
SEDIEBB79_02330(ccrA)
BOOE2K80_14180(ccrA)
PSEBEOK75_09460(ccrA)
PPRUFDP22_03530(ccrA)
PAEDG5B38_14070(ccrA)
MONG8E03_02130(ccrA)
PAMOBAR1_15085(ccrA)
PSHQF3W81_20350(ccrA)
POZI0K15_10720(ccrA)
PALWPSAL_018850(ccr)
PGVSL003B_0355
CACTHZ995_03590(ccrA)
THASC6Y53_10565(ccrA)
HDHG5B40_19100(ccrA)
CMAGCBW24_14080
GCEKYE46_04130(ccrA)
NSMJO391_13150(ccrA)
ACROK3J57_10545(ccrA)
SINRO5O51_13235(ccrA)
FAQG5B39_05195(ccrA)
AMAQGO499_14275(ccrA)
HNEHNE_3118(ccrA)
HBAHbal_0288
CBOTATE48_04265
HBCAEM38_09265
SKTIGS68_10660(ccrA)
SMUCJL100_016570(ccrA)
SROEJL101_014995(ccrA)
RRURru_A3063
RRFF11_15690
RPMRSPPHO_00638
MAGamb0309
MGYMGMSRv2__2674
MGRYMSR1_36920(ccrA2)
MAGXXM1_1237(ccr)
MAGNWV31_14300
FERFNB15_00565(ccrA)
HTQFRZ44_46290
HADHFRZ61_01130(adhP) FRZ61_44220
DEXHWD60_01570(ccrA)
DVNHQ394_15000(ccrA)
TMOTMO_0119
TXITH3_17595
THACCSC3H3_16685(ccrA)
TIIDY252_04210(ccrA)
THAIIT893_16600(ccrA)
MAGQMGMAQ_3100
ECOGFIV45_15325(ccrA)
SNEANBZ79_17900(ccrA)
PSOPKP014_14685(ccrA)
CSRCspa_c03820(ccr)
RCHRUM_00790
NTPCRH09_38255(ccrA)
ROPROP_72100
RKOJWS14_45090(ccrA) JWS14_48900(ccrA)
SALBXNR_5889
SMASAVERM_414(pteB)
SRCM271_43880
SBHSBI_01373
SAMBSAM23877_0426
SCXAS200_43230
STRFASR50_01505
SKYD0C37_31705(ccrA)
SPHWNFX46_22155(ccrA)
SPLULK06_031365
SPUNBFF78_04530
SLAUSLA_0406 SLA_7484
SRJSRO_7228
SASTCD934_28935(ccrA)
SVNCP980_28480(ccrA)
SRKFGW37_32970(ccrA)
SGFHEP81_07556(ccrA_2)
SFUGCNQ36_01460(ccrA)
SPACB1H29_00485 B1H29_36575
SATAC5746_38515(ccrA)
SMOBJ7W19_12560(ccrA)
SPEUCGZ69_08265(ccrA) CGZ69_28875(ccrA)
SGJIAG43_33070(ccrA)
SHKJ2N69_01060(ccrA)
SDDD9753_11255(ccrA)
SACTDMT42_00480(ccrA)
SRUGF0345_01225(ccrA)
SAKBK1J60_45170(ccrA)
AUSIPK37_16815(ccrA)
NMESH9L09_18030(ccrA)
NDANdas_0488
NALB005_3356
NEXNE857_08735(ccrA)
AORISD37_26720
PSUUPsuf_066920(adhP_1) Psuf_073520(adhP_2)
BSOLFSW04_11630(ccrA)
CWOCwoe_4182
PARNNBH00_07305(ccrA)
SBAEDSM104329_01844(ccr)
TBHTbon_11600(ccrA)
LILLA_0296(adhP)
LIELIF_A0249(adhP)
LICLIC_10253
LISLIL_10251(adhP1)
LBJLBJ_2607
LBLLBL_0505
LBILEPBI_I3117
LBFLBF_3008
LSTLSS_00520
LAJA0128_16875
LMAYDPV73_07740(ccrA)
LKMEFP84_08590(ccrA)
LWLFHG68_03355(ccrA)
LKBLPTSP3_g32730(adhP_3)
LNOMAL08_01270(ccrA)
TPXTurpa_4007
CTMCabther_B0115
COYHF329_03875(ccrA)
 » show all
Reference
1  [PMID:17548827]
  Authors
Erb TJ, Berg IA, Brecht V, Muller M, Fuchs G, Alber BE
  Title
Synthesis of C5-dicarboxylic acids from C2-units involving crotonyl-CoA carboxylase/reductase: the ethylmalonyl-CoA pathway.
  Journal
Proc Natl Acad Sci U S A 104:10631-6 (2007)
DOI:10.1073/pnas.0702791104
  Sequence
[rsp:RSP_0960]
Reference
2  [PMID:19458256]
  Authors
Erb TJ, Brecht V, Fuchs G, Muller M, Alber BE
  Title
Carboxylation mechanism and stereochemistry of crotonyl-CoA carboxylase/reductase, a carboxylating enoyl-thioester reductase.
  Journal
Proc Natl Acad Sci U S A 106:8871-6 (2009)
DOI:10.1073/pnas.0903939106
Other DBs
ExplorEnz - The Enzyme Database: 1.3.1.85
IUBMB Enzyme Nomenclature: 1.3.1.85
ExPASy - ENZYME nomenclature database: 1.3.1.85
BRENDA, the Enzyme Database: 1.3.1.85
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