KEGG   ENZYME: 1.3.7.15
Entry
EC 1.3.7.15                 Enzyme                                 
Name
chlorophyllide a reductase;
bchX (gene name);
bchY (gene name);
bchZ (gene name);
COR
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With an iron-sulfur protein as acceptor
Sysname
bacteriochlorophyllide-a:ferredoxin 7,8-oxidoreductase
Reaction(IUBMB)
(1) 3-deacetyl-3-vinylbacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate = chlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+ [RN:R09053];
(2) bacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate = 3-acetyl-3-devinylchlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+;
(3) 3-deacetyl-3-(1-hydroxyethyl)bacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate = 3-devinyl-3-(1-hydroxyethyl)chlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+ [RN:R09060]
Reaction(KEGG)
R09053 R09060
Substrate
3-deacetyl-3-vinylbacteriochlorophyllide a [CPD:C18152];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139];
ADP [CPD:C00008];
phosphate [CPD:C00009];
bacteriochlorophyllide a [CPD:C18155];
3-deacetyl-3-(1-hydroxyethyl)bacteriochlorophyllide a [CPD:C18153]
Product
chlorophyllide a [CPD:C02139];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138];
ATP [CPD:C00002];
H2O [CPD:C00001];
H+ [CPD:C00080];
3-acetyl-3-devinylchlorophyllide a;
3-devinyl-3-(1-hydroxyethyl)chlorophyllide a [CPD:C18154]
Comment
The enzyme, together with EC 1.1.1.396, bacteriochlorophyllide-a dehydrogenase, and EC 4.2.1.165, chlorophyllide-a 31-hydratase, is involved in the conversion of chlorophyllide a to bacteriochlorophyllide a. These enzymes can act in multiple orders, resulting in the formation of different intermediates, but the final product of the cumulative action of the three enzymes is always bacteriochlorophyllide a. This enzyme catalyses a trans-reduction of the B-ring; the product has the (7R,8R)-configuration. In addition, the enzyme has a latent activity of EC 1.3.7.13, 3,8-divinyl protochlorophyllide a 8-vinyl-reductase (ferredoxin) [4]. The enzyme contains a [4Fe-4S] cluster, and structurally resembles the Fe protein/MoFe protein complex of nitrogenase (EC 1.18.6.1), which catalyses an ATP-driven reduction.
History
EC 1.3.7.15 created 1965 as EC 1.3.99.35, modified 2012, transferred 2016 to EC 1.3.7.15
Pathway
ec00860  Porphyrin metabolism
ec01110  Biosynthesis of secondary metabolites
Orthology
K11333  3,8-divinyl chlorophyllide a/chlorophyllide a reductase subunit X
K11334  3,8-divinyl chlorophyllide a/chlorophyllide a reductase subunit Y
K11335  3,8-divinyl chlorophyllide a/chlorophyllide a reductase subunit Z
Genes
ALVAlvin_2556 Alvin_2561 Alvin_2562
ATEPAtep_25090 Atep_25140 Atep_25150
TVIThivi_2003 Thivi_2010 Thivi_2011
TMBThimo_3003 Thimo_3004 Thimo_3005
MPURMARPU_13265 MARPU_13270 MARPU_13275
TSYTHSYN_31095 THSYN_31100 THSYN_31105
TTPE6P07_01740 E6P07_01745(bchY) E6P07_01790(bchZ)
THIPN838_12395 N838_12400(bchY) N838_12405(bchZ)
CJAPGWK36_12630(bchZ) GWK36_12635(bchY) GWK36_12640
THIMKFB96_13090(bchZ) KFB96_13095(bchY) KFB96_13100
TBOGLT988_08895(bchZ) LT988_08900(bchY) LT988_08905
HHAHhal_1608 Hhal_1609 Hhal_1610
HHKHH1059_06270 HH1059_06280(bchY) HH1059_06290
HHCM911_10340 M911_10345 M911_10350
EBSECTOBSL9_3044 ECTOBSL9_3045 ECTOBSL9_3046
PDQCL55_00013570 CL55_00013580 CL55_00013590
RACRA876_06000 RA876_06005 RA876_06010
LIML103DPR2_00972(nifH1) L103DPR2_00973(bchN_1) L103DPR2_00974(chlB_1)
LIHL63ED372_00628(nifH1) L63ED372_00629(bchN_1) L63ED372_00630(chlB)
HYRBSY239_1379(bchZ) BSY239_1380(bchY) BSY239_1381
METPC1M51_03565(bchZ) C1M51_03570(bchY) C1M51_03575
RGERGE_33680(bchZ) RGE_33690(bchY) RGE_33700(bchX)
RBNRBXJA2T_09442 RBXJA2T_09447 RBXJA2T_09452
RDPRD2015_1037 RD2015_1038 RD2015_1039
ATERMW290_02510(bchZ) MW290_02515(bchY) MW290_02520
METRBSY238_2448 BSY238_2449(bchY) BSY238_2450(bchZ)
BEBAEM42_07475 AEM42_07480 AEM42_07485
RPODE0E05_11790 E0E05_11795(bchY) E0E05_11800(bchZ)
AGCBSY240_3666(bchZ) BSY240_3667(bchY) BSY240_3668
ABAWD5400_04820(bchZ) D5400_04825(bchY) D5400_04830
PDESFE840_002325 FE840_002330(bchY) FE840_002335(bchZ)
BRABRADO1618(bchX) BRADO1619(bchY) BRADO1620(bchZ)
BBTBBta_6435(bchZ) BBta_6436(bchY) BBta_6437(bchX)
BRSS23_19180(bchX) S23_19190(bchY) S23_19200(bchZ)
AOLS58_59160 S58_59170 S58_59180
BROBRAD285_5779(bchZ) BRAD285_5780(bchY) BRAD285_5781(bchX)
BRQCIT40_23930(bchZ) CIT40_23935(bchY) CIT40_23940
BGZXH91_02645 XH91_02650(bchY) XH91_02655(bchZ)
BBETF8237_24675 F8237_24680(bchY) F8237_24685(bchZ)
RPARPA1522(bchX) RPA1523(bchY) RPA1524(bchZ)
RPBRPB_3999 RPB_4000 RPB_4001
RPCRPC_1293 RPC_1294 RPC_1295
RPDRPD_3754 RPD_3755 RPD_3756
RPERPE_1327 RPE_1328 RPE_1329
RPTRpal_1710 Rpal_1711 Rpal_1712
RPXRpdx1_3996 Rpdx1_3997 Rpdx1_3998
BOSBSY19_4053(bchZ) BSY19_4054(bchY) BSY19_4055
MEAMex_1p2927(bchX) Mex_1p2928(bchY) Mex_1p2929(bchZ)
MDIMETDI3496(bchX) METDI3497(bchY) METDI3498(bchZ)
MEXMext_2730 Mext_2731 Mext_2732
MCHMchl_2957 Mchl_2958 Mchl_2959
MPOMpop_2852 Mpop_2853 Mpop_2854
MZAB2G69_21190 B2G69_21195 B2G69_21200
MRDMrad2831_2816 Mrad2831_2817 Mrad2831_2818
METM446_3697 M446_3698 M446_3699
MORMOC_3181(bchZ) MOC_3182(bchY) MOC_3183
METAY590_13140 Y590_13145 Y590_13150
MAQUMaq22A_1p32545(nifH) Maq22A_1p32550 Maq22A_1p32555(nifD)
MPHYMCBMB27_02257 MCBMB27_02258 MCBMB27_02259
MEEDA075_03960 DA075_03965(bchY) DA075_03970(bchZ)
METDC0214_15305 C0214_15310(bchY) C0214_15315(bchZ)
METXA3862_17700 A3862_17705 A3862_17710
METSDK389_31425(bchZ) DK389_31430(bchY) DK389_31435
METIDK427_17885(bchZ) DK427_17890(bchY) DK427_17895
MMESMMSR116_13615 MMSR116_13620(bchY) MMSR116_13625(bchZ)
MTEADK419_04920 DK419_04925(bchY) DK419_04930(bchZ)
MINDmvi_08980 mvi_08990(bchY) mvi_09000
MOGMMB17_23875(bchZ) MMB17_23880(bchY) MMB17_23885
MSLMsil_2052 Msil_2053 Msil_2054
BBARRHAL1_03012(bchZ) RHAL1_03013(bchY) RHAL1_03014(bchX)
RVARvan_0210 Rvan_0211 Rvan_0212
BVRBVIR_599 BVIR_600 BVIR_601
BLAGBLTE_02350 BLTE_02360 BLTE_02370
MROSEHO51_17035 EHO51_17040(bchY) EHO51_17045(bchZ)
CMETK6K41_01675 K6K41_01680(bchY) K6K41_01685(bchZ)
AUBLXB15_06370(bchZ) LXB15_06375(bchY) LXB15_06380
BSBBresu_3043 Bresu_3044 Bresu_3045
BRLBZG35_15445 BZG35_15450 BZG35_15455
RUTFIU92_10145(chlB) FIU92_10150 FIU92_10155(nifH1)
JANJann_0177(bchZ) Jann_0178(bchY) Jann_0179(bchX)
RDERD1_0109(bchZ) RD1_0110(bchY) RD1_0111(bchX)
RLIRLO149_p940160(bchX) RLO149_p940170(bchY) RLO149_p940180(bchZ)
RPONG3256_18660 G3256_18670(bchY) G3256_18675(bchZ)
DSHDshi_3517(bchX) Dshi_3518(bchY) Dshi_3519(bchZ)
REDroselon_01078 roselon_01079 roselon_01080
SUAMBOO69_19400 BOO69_19405 BOO69_19410
TOMBWR18_20240 BWR18_20250 BWR18_20255
RMMROSMUCSMR3_00902(bchZ) ROSMUCSMR3_00903(bchY) ROSMUCSMR3_00905(bchX)
ROKRAK1035_3771 RAK1035_3772 RAK1035_3774
RIDRIdsm_01755(nifH1) RIdsm_01757 RIdsm_01758(chlB)
ROMEI983_05440 EI983_05445(bchY) EI983_05450(bchZ)
ROHFIU89_14220(chlB) FIU89_14225 FIU89_14235(nifH1)
THAACFI11_23550 CFI11_23555(bchY) CFI11_23560(bchZ)
MARUFIU81_13850(chlB) FIU81_13855(chlN) FIU81_13860(nifH1)
ROTFIV09_18070(nifH1) FIV09_18075 FIV09_18080(chlB)
MALUKU6B_16430(bchX) KU6B_16440(bchY) KU6B_16450(bchZ)
RSPRSP_0260(bchZ) RSP_0261(bchY) RSP_0262(bchX)
RSHRsph17029_1904 Rsph17029_1905 Rsph17029_1906
RSQRsph17025_2034 Rsph17025_2035 Rsph17025_2036
RSKRSKD131_1587 RSKD131_1588 RSKD131_1589
CAZTLV780_09065(bchZ) LV780_09070(bchY) LV780_09075
RCPRCAP_rcc00687(bchX) RCAP_rcc00688(bchY) RCAP_rcc00689(bchZ)
RBLB6K69_00190 B6K69_00195 B6K69_00200
PTPRCA23_c29620(bchX) RCA23_c29630(bchY) RCA23_c29640(bchZ)
RSUNHU_04287(bchX) NHU_04289(bchY) NHU_04290(bchZ)
RHMB5V46_17475 B5V46_17480 B5V46_17485
RHCRGUI_1959 RGUI_1960 RGUI_1961
LVSLOKVESSMR4R_03679(bchX) LOKVESSMR4R_03680(bchY) LOKVESSMR4R_03681(bchZ)
RBGBG454_03035 BG454_03040(bchY) BG454_03045(bchZ)
GEHHYN69_05525 HYN69_05530(bchY) HYN69_05535(bchZ)
TAWEI545_18945 EI545_18950(bchY) EI545_18955(bchZ)
POZI0K15_00010 I0K15_20930(bchZ)
NSMJO391_16465 JO391_16470(bchY) JO391_16475(bchZ)
FAQG5B39_11870(bchZ) G5B39_11875(bchY) G5B39_11880
HBCAEM38_14575 AEM38_14580 AEM38_14585
NOTC7W88_00480 C7W88_00490(bchZ)
NGFFRF71_09145 FRF71_09150(bchY) FRF71_09155(bchZ)
NOGGKE62_08435 GKE62_08440(bchY) GKE62_08445(bchZ)
SPHITS85_22905 TS85_22910 TS85_22915
SSANNX02_18440 NX02_18445 NX02_18450
SCHYGVO57_11620(bchZ) GVO57_11625(bchY) GVO57_11630
CIJWG74_05045 WG74_05050 WG74_05055
BLASBSY18_3555 BSY18_3556(bchY) BSY18_3557(bchZ)
BFWB5J99_08570 B5J99_08575 B5J99_08580
PALGHFP57_06940(bchZ) HFP57_06945(bchY) HFP57_06950
PARJJ4G78_07525(bchZ) J4G78_07530(bchY) J4G78_07535
SANDH3309_05750 H3309_05755(bchY) H3309_05760(bchZ)
POQKZX46_18695(bchZ) KZX46_18700(bchY) KZX46_18705
ALHG6N82_11430 G6N82_11435(bchY) G6N82_11440(bchZ)
ELQGa0102493_111836 Ga0102493_111837 Ga0102493_111838
ERKCD351_13870 CD351_13875(bchY) CD351_13880(bchZ)
ERFFIU90_13830(nifH1) FIU90_13835 FIU90_13840(chlB1)
PNSA9D12_01290 A9D12_01295 A9D12_01300
PORLBG023_112670 BG023_112671 BG023_112672
PHZCHX26_14500 CHX26_14505(bchY) CHX26_14510(bchZ)
POTE2E27_07325(bchZ) E2E27_07330(bchY) E2E27_07335
AMVACMV_18770(bchZ) ACMV_18780(bchY) ACMV_18790(bchX)
SRFLHU95_13855 LHU95_13860(bchY) LHU95_13865(bchZ)
RAPRHOA_2428(bchX) RHOA_2429(bchY) RHOA_2430(bchZ)
NCBC0V82_18155(bchZ) C0V82_18160(bchY) C0V82_18165
SKTIGS68_17105(bchZ) IGS68_17110(bchY) IGS68_17115
SMUCJL100_008920 JL100_008925(bchY) JL100_008930(bchZ)
SROEJL101_015285(bchZ) JL101_015290(bchY) JL101_015295
RRURru_A2978 Rru_A2979 Rru_A2980
RRFF11_15255 F11_15260 F11_15265
RCERC1_2094(bchX) RC1_2095(bchY) RC1_2096(bchZ)
RPMRSPPHO_01498(bchZ) RSPPHO_01499(bchY) RSPPHO_01500
ELIOKO353_03065(bchZ) KO353_03070(bchY) KO353_03075
HMOHM1_0654(bchY) HM1_0655(bchZ) HM1_0659(bchX)
HCVFTV88_2670(bchX) FTV88_2673(bchZ) FTV88_2674(bchY)
RRSRoseRS_3256 RoseRS_3257 RoseRS_3260
RCARcas_3743 Rcas_3744 Rcas_3746
CAUCaur_0417 Caur_3805 Caur_3806
CHLChy400_0444 Chy400_4108 Chy400_4109
CAGCagg_0850 Cagg_0851 Cagg_3127
CTMCabther_A0934 Cabther_A1527 Cabther_A1571
CVLJ8C06_01345 J8C06_07320 J8C06_09555
CHLOJ8C02_04520 J8C02_07535 J8C02_07755
GPHGEMMAAP_06510 GEMMAAP_06515 GEMMAAP_06520
GGRHKW67_08765 HKW67_08770(bchY) HKW67_08775(bchZ)
CTECT1423(bchX) CT1826(bchY) CT2125(bchZ)
CPCCpar_0119 Cpar_0375 Cpar_0741
CLZBIU88_03600 BIU88_09875 BIU88_11835
CCHCag_0324 Cag_0400 Cag_1716
CPHCpha266_1792 Cpha266_2075 Cpha266_2500
CPBCphamn1_0173 Cphamn1_0937 Cphamn1_2062
CLIClim_0157 Clim_0897 Clim_2035
PVICvib_0191 Cvib_0407 Cvib_1241
PLTPlut_0124 Plut_0342 Plut_1423
PPHPpha_0177 Ppha_0979 Ppha_2411
PAAPaes_0168 Paes_1535 Paes_1863
PROCPtc2401_00130(bchB_1) Ptc2401_00667(nifH1_1) Ptc2401_01952
PRSB9H02_00755 B9H02_04010
PROSCHL67_00825(bchZ) CHL67_03600 CHL67_09910(bchY)
CTSCtha_0198 Ctha_0817 Ctha_2720
 » show all
Reference
1  [PMID:16571720]
  Authors
Nomata J, Mizoguchi T, Tamiaki H, Fujita Y
  Title
A second nitrogenase-like enzyme for bacteriochlorophyll biosynthesis: reconstitution of chlorophyllide a reductase with purified X-protein (BchX) and YZ-protein (BchY-BchZ) from Rhodobacter capsulatus.
  Journal
J Biol Chem 281:15021-8 (2006)
DOI:10.1074/jbc.M601750200
  Sequence
Reference
2  [PMID:23386973]
  Authors
Tsukatani Y, Yamamoto H, Harada J, Yoshitomi T, Nomata J, Kasahara M, Mizoguchi T, Fujita Y, Tamiaki H
  Title
An unexpectedly branched biosynthetic pathway for bacteriochlorophyll b capable of absorbing near-infrared light.
  Journal
Sci Rep 3:1217 (2013)
DOI:10.1038/srep01217
Reference
3  [PMID:26088139]
  Authors
Lange C, Kiesel S, Peters S, Virus S, Scheer H, Jahn D, Moser J
  Title
Broadened Substrate Specificity of 3-Hydroxyethyl Bacteriochlorophyllide a Dehydrogenase (BchC) Indicates a New Route for the Biosynthesis of Bacteriochlorophyll a.
  Journal
J Biol Chem 290:19697-709 (2015)
DOI:10.1074/jbc.M115.660555
Reference
4  [PMID:24637023]
  Authors
Harada J, Mizoguchi T, Tsukatani Y, Yokono M, Tanaka A, Tamiaki H
  Title
Chlorophyllide a oxidoreductase works as one of the divinyl reductases specifically involved in bacteriochlorophyll a biosynthesis.
  Journal
J Biol Chem 289:12716-26 (2014)
DOI:10.1074/jbc.M113.546739
Other DBs
ExplorEnz - The Enzyme Database: 1.3.7.15
IUBMB Enzyme Nomenclature: 1.3.7.15
ExPASy - ENZYME nomenclature database: 1.3.7.15
BRENDA, the Enzyme Database: 1.3.7.15
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