KEGG   ENZYME: 1.3.99.30
Entry
EC 1.3.99.30                Enzyme                                 
Name
phytoene desaturase (3,4-didehydrolycopene-forming);
5-step phytoene desaturase;
five-step phytoene desaturase;
phytoene desaturase (ambiguous);
Al-1
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With unknown physiological acceptors
Sysname
15-cis-phytoene:acceptor oxidoreductase (3,4-didehydrolycopene-forming)
Reaction(IUBMB)
15-cis-phytoene + 5 acceptor = all-trans-3,4-didehydrolycopene + 5 reduced acceptor (overall reaction) [RN:R09703];
(1a) 15-cis-phytoene + acceptor = all-trans-phytofluene + reduced acceptor [RN:R09692];
(1b) all-trans-phytofluene + acceptor = all-trans-zeta-carotene + reduced acceptor [RN:R04787];
(1c) all-trans-zeta-carotene + acceptor = all-trans-neurosporene + reduced acceptor [RN:R04798];
(1d) all-trans-neurosporene + acceptor = all-trans-lycopene + reduced acceptor [RN:R04800];
(1e) all-trans-lycopene + acceptor = all-trans-3,4-didehydrolycopene + reduced acceptor [RN:R07518]
Reaction(KEGG)
R04787 R04798 R04800 R07518 R09692 R09703
Substrate
15-cis-phytoene [CPD:C05421];
acceptor [CPD:C00028];
all-trans-phytofluene [CPD:C05414];
all-trans-zeta-carotene [CPD:C05430];
all-trans-neurosporene [CPD:C05431];
all-trans-lycopene [CPD:C05432]
Product
all-trans-3,4-didehydrolycopene [CPD:C15867];
reduced acceptor [CPD:C00030];
all-trans-phytofluene [CPD:C05414];
all-trans-zeta-carotene [CPD:C05430];
all-trans-neurosporene [CPD:C05431];
all-trans-lycopene [CPD:C05432]
Comment
This enzyme is involved in carotenoid biosynthesis and catalyses up to five desaturation steps (cf. EC 1.3.99.28 [phytoene desaturase (neurosporene-forming)], EC 1.3.99.29 [phytoene desaturase (zeta-carotene-forming)] and EC 1.3.99.31 [phytoene desaturase (lycopene-forming)]).
History
EC 1.3.99.30 created 2011
Pathway
ec00906  Carotenoid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K15745  phytoene desaturase (3,4-didehydrolycopene-forming)
Genes
CNS: 116339530 116349508
BCOO: 119078125
AGIF: 122854590 122854592 122854593
API: 100159050 100161380 100169110 100169245(tor)
DNX: 107165446
AGS: 114125979
RMD: 113548062 113549968
ACOO: 126834667 126834668
DVT: 126905570
VJA: 111273439
TUT: 107364307 107364309 107365911
VCN: VOLCADRAFT_96696
MNG: MNEG_6064
CVR: CHLNCDRAFT_48542
OLU: OSTLU_29222
OTA: OT_ostta01g05990
BPG: Bathy15g02080
MIS: MICPUN_85926
MPP: MICPUCDRAFT_36095
NCR: NCU00552(al-1)
NTE: NEUTE1DRAFT92886(NEUTE1DRAFT_92886)
SMP: 10810047(SMAC4_01244)
PAN: PODANSg3970
PBEL: QC761_115010(AL1)
PPSD: QC762_115010(AL1)
PPSP: QC763_115010(AL1)
PPSA: QC764_115010(AL1)
FGR: FGSG_03065
FPU: FPSE_02161
FPOA: FPOAC1_005422
FVN: FVRRES_05979
FVR: FVEG_10717
FOX: FOXG_12143
NHE: NECHADRAFT_97347
FFC: NCS54_01343900
FKR: NCS57_01320600
FMU: J7337_013306
VAL: VDBG_00708
VDA: VDAG_00320
CFJ: CFIO01_11136
CLUP: CLUP02_08750
CHIG: CH63R_07461
CDET: 87944659(CDEST_08156)
ELA: UCREL1_7363
PFY: PFICI_09859
SSL: SS1G_04340
BFU: BCIN_01g04550(Bcphd1)
MBE: MBM_00660
PSCO: LY89DRAFT_686853
GLZ: GLAREA_08046
AOR: AO090020000158
AFV: AFLA_010500
ALUC: AKAW2_12022A AKAW2_61045S AKAW2_61046A
POU: POX_a01893 POX_a01895
ABE: ARB_03950
TVE: TRV_03238
PNO: SNOG_00342
PTE: PTT_09941
PTRR: 6345636(PtrM4_040010)
BZE: COCCADRAFT_96840
BSC: COCSADRAFT_304602
BOR: COCMIDRAFT_88940
AALT: CC77DRAFT_951686
ARAB: EKO05_0004494
ZTR: MYCGRDRAFT_101921
PFJ: MYCFIDRAFT_29348
FFU: CLAFUR5_14250
CBET: CB0940_02240
BCOM: BAUCODRAFT_120791
NPA: UCRNP2_7258
KMG: 30160148(I203_104720)
KNE: 92180956(IAR55_003698)
TMS: TREMEDRAFT_34017
SHS: STEHIDRAFT_99350
UMA: UMAG_04210
PFP: PFL1_06723
SGRA: EX895_001661
PGR: PGTG_19044
PTRC: PtA15_8A421
PSTR: Pst134EA_015845
MLR: MELLADRAFT_40795
ACAN: ACA1_391690
PTI: PHATR_43904
FCY: FRACYDRAFT_195646(PDH1) FRACYDRAFT_231974
EHX: EMIHUDRAFT_458688
PBF: CFX0092_B0152
 » show all
Reference
1  [PMID:11017770]
  Authors
Hausmann A, Sandmann G
  Title
A single five-step desaturase is involved in the carotenoid biosynthesis pathway to beta-carotene and torulene in Neurospora crassa.
  Journal
Fungal Genet Biol 30:147-53 (2000)
DOI:10.1006/fgbi.2000.1212
  Sequence
[ncr:NCU00552]
Reference
2  [PMID:18812228]
  Authors
Estrada AF, Maier D, Scherzinger D, Avalos J, Al-Babili S
  Title
Novel apocarotenoid intermediates in Neurospora crassa mutants imply a new biosynthetic reaction sequence leading to neurosporaxanthin formation.
  Journal
Fungal Genet Biol 45:1497-505 (2008)
DOI:10.1016/j.fgb.2008.09.001
  Sequence
[ncr:NCU00552]
Other DBs
ExplorEnz - The Enzyme Database: 1.3.99.30
IUBMB Enzyme Nomenclature: 1.3.99.30
ExPASy - ENZYME nomenclature database: 1.3.99.30
BRENDA, the Enzyme Database: 1.3.99.30
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