KEGG   ENZYME: 1.4.2.3
Entry
EC 1.4.2.3                  Enzyme                                 
Name
pseudooxynicotine dehydrogenase;
pnaO (gene name)
Class
Oxidoreductases;
Acting on the CH-NH2 group of donors;
With a cytochrome as acceptor
Sysname
4-(methylamino)-1-(pyridin-3-yl)butan-1-one:c-type cytochrome oxidoreductase (methylamine releasing)
Reaction(IUBMB)
pseudooxynicotine + H2O + 2 ferricytochrome c = 4-oxo-4-(pyridin-3-yl)butanal + methylamine + 2 ferrocytochrome c + 2 H+ [RN:R10103]
Reaction(KEGG)
R10103
Substrate
pseudooxynicotine [CPD:C20361];
H2O [CPD:C00001];
ferricytochrome c [CPD:C00125]
Product
4-oxo-4-(pyridin-3-yl)butanal [CPD:C19567];
methylamine [CPD:C00218];
ferrocytochrome c [CPD:C00126];
H+ [CPD:C00080]
Comment
Contains one non-covalently bound FAD molecule per dimer. This enzyme, characterized from the soil bacteria Pseudomonas sp. HZN6 and Pseudomonas putida S16, is involved in nicotine degradation.
History
EC 1.4.2.3 created 2012 as EC 1.4.3.24, transferred 2022 to EC 1.4.2.3
Pathway
ec00760  Nicotinate and nicotinamide metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K20158  pseudooxynicotine dehydrogenase
Genes
PPT: PPS_4080
PMON: X969_19990
PMOT: X970_19625
PMED: E3Z27_20800
PVK: EPZ47_21950
PSJY: AA098_03980 AA098_04095 AA098_21420
ALTR: BM528_03545
CUK: KB879_35300
PPAI: E1956_19245
RHOJ: JVH1_17505
Reference
1  [PMID:22267672]
  Authors
Qiu J, Ma Y, Wen Y, Chen L, Wu L, Liu W
  Title
Functional identification of two novel genes from Pseudomonas sp. strain HZN6 involved in the catabolism of nicotine.
  Journal
Appl Environ Microbiol 78:2154-60 (2012)
DOI:10.1128/AEM.07025-11
  Sequence
[ag:AFD54463]
Reference
2  [PMID:35835223]
  Authors
Choudhary V, Wu K, Zhang Z, Dulchavsky M, Barkman T, Bardwell JCA, Stull F.
  Title
The enzyme pseudooxynicotine amine oxidase from Pseudomonas putida S16 is not an oxidase, but a dehydrogenase.
  Journal
J Biol Chem 298:102251 (2022)
DOI:10.1016/j.jbc.2022.102251
  Sequence
[ppt:PPS_4080]
Other DBs
ExplorEnz - The Enzyme Database: 1.4.2.3
IUBMB Enzyme Nomenclature: 1.4.2.3
ExPASy - ENZYME nomenclature database: 1.4.2.3
BRENDA, the Enzyme Database: 1.4.2.3
LinkDB

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