KEGG ENZYME: 1.5.3.10

ENZYME: 1.5.3.10

Entry

EC 1.5.3.10                 Enzyme

Name

dimethylglycine oxidase;
dmg (gene name);
N,N-dimethylglycine:oxygen oxidoreductase (demethylating)

Class

Oxidoreductases;
Acting on the CH-NH group of donors;
With oxygen as acceptor

Sysname

N,N-dimethylglycine,5,6,7,8-tetrahydrofolate:oxygen oxidoreductase (demethylating,5,10-methylenetetrahydrofolate-forming)

Reaction(IUBMB)

N,N-dimethylglycine + 5,6,7,8-tetrahydrofolate + O2 = sarcosine + 5,10-methylenetetrahydrofolate + H2O2 [RN:R01564]

Reaction(KEGG)

R01564

Substrate

N,N-dimethylglycine [CPD:C01026];
5,6,7,8-tetrahydrofolate [CPD:C00101];
O2 [CPD:C00007]

Product

sarcosine [CPD:C00213];
5,10-methylenetetrahydrofolate [CPD:C00143];
H2O2 [CPD:C00027]

Comment

A flavoprotein (FAD). The enzyme, characterized from the bacterium Arthrobacter globiformis, contains two active sites connected by a large "reaction chamber". An imine intermediate is transferred between the sites, eliminating the production of toxic formaldehyde. In the absence of folate the enzyme does form formaldehyde. Does not oxidize sarcosine. cf. EC 1.5.8.4, dimethylglycine dehydrogenase.

History

EC 1.5.3.10 created 1992, modified 2022

Pathway

ec00260

Glycine, serine and threonine metabolism

ec01100

Metabolic pathways

Orthology

K00309

dimethylglycine oxidase

Genes

MGR:

MGG_12421

PPEI:

PpBr36_02012

PGRI:

PgNI_07803

TMN:	UCRPA7_1978

FGR:	FGSG_03414

FPU:	FPSE_08388

FPOA:

FPOAC1_005794

FVN:	FVRRES_06392

FVR:	FVEG_10784

FOX:	FOXG_12340

NHE:	NECHADRAFT_51705

FFC:	NCS54_01325500

FKR:	NCS57_01312700

FMU:	J7337_013376

MAJ:

MAA_00906

MBRN:

26246997(dmg)

PCHM:

VFPPC_10841

CMT:

CCM_08027

AMUS:

LMH87_010702

PLJ:	28891846(PLICBS_003991)

PTKZ:

JDV02_001997

VDA:	VDAG_05053

CFJ:	CFIO01_00388

CLUP:

CLUP02_17239

CHIG:

CH63R_09496

PFY:	PFICI_10893

ANI:	ANIA_08654

AFM:	AFUA_8G06470

NFI:	NFIA_099100

AOR:	AO090023000142

ANG:	An15g02590

AFV:	AFLA_006506

ALUC:

AKAW2_60437S

ACHE:

ACHE_20201S

APUU:

APUU_10234S

PCS:	N7525_003723

PDP:	PDIP_84880

POU:	POX_a01473

TRG:	TRUGW13939_11773

PNO:	SNOG_10285

PTE:

PTT_07671

PTRR:

6344165(PtrM4_103950)

BZE:	COCCADRAFT_109688

BSC:	COCSADRAFT_199303

BOR:	COCMIDRAFT_83186

AALT:

CC77DRAFT_718683

ARAB:

EKO05_0005261

ZTR:	MYCGRDRAFT_91876

PFJ:	MYCFIDRAFT_81948

FFU:	CLAFUR5_13648

CBET:

CB0940_09813

VIT:	ADP71_15950

VST:	LVJ81_05585

PLEO:

OHA_1_00894(mlr_2)

APC:	HIMB59_00006330

BMQ:

BMQ_1539

BMD:

BMD_1520

BMH:	BMWSH_3690

BMEG:

BG04_3831(dmg)

VNT:	OLD84_07640

PBJ:	VN24_07415

MSA:	Mycsm_03756

MMI:	MMAR_4420(gcvT_2)

MMAE:

MMARE11_42450(gcvT_2)

MLI:	MULP_04640(gcvT_2)

MSHO:

MSHO_02100

MMC:

Mmcs_2459

MKM:

Mkms_2504

MJL:

Mjls_2496

MBAI:

MB901379_03869(gcvT_3)

MYW:	BVC93_13595

MGI:

Mflv_3080

MSP:	Mspyr1_23950

MCB:	Mycch_3087

MVQ:

MYVA_3366

MAUU:

NCTC10437_03331(gcvT_3)

MMAG:

MMAD_49080

MTY:	MTOK_13900

MPSC:

MPSYJ_32520

MARZ:

MARA_42230

MPOF:

MPOR_36250

MMAT:

MMAGJ_50970

MSEI:

MSEDJ_08720

MMON:

EWR22_12495

MRF:	MJO55_15790

MGRO:

FZ046_07905

ASD:	AS9A_0968 AS9A_1005

CVA:

CVAR_2042

CTER:

A606_08170

CPRE:

Csp1_08840(dmg)

NBR:	O3I_018395

NTP:	CRH09_17885

NOZ:	DMB37_02150

NAH:	F5544_24870

NYA:	LTV02_18390

RHA:	RHA1_ro01815

ROP:

ROP_14920

ROA:	Pd630_LPD05975

RHB:

NY08_3778

RKO:	JWS14_09300 JWS14_16740

ROZ:	CBI38_23385

RPSK:

JWS13_36545

RHOP:

D8W71_00175

RHOJ:

JVH1_28260

RFA:	A3L23_02135(dmg_1)

RHS:	A3Q41_01248(dmg)

WHR:	OG579_01005

GPO:	GPOL_c02700

GOR:

KTR9_1190

GOM:	D7316_03170(dmg)

GOS:	QAD21_06200

GOJ:	GR168_06080

GAM:	GII34_03040

GOI:	LK459_08500

GHN:	MVF96_06420

GMG:	NWF22_10500

SRT:

Srot_2474

SMA:	SAVERM_6951

SGRF:

SGFS_011370

SCB:

SCAB_6961

SVL:	Strvi_5720

SHY:

SHJG_2829

SHO:	SHJGH_2593

SRC:	M271_43595

SMAL:

SMALA_0689

SSOI:

I1A49_07040

SBH:

SBI_00369

SDV:	BN159_7405

SALS:

SLNWT_1480

SVT:	SVTN_37030

STRE:

GZL_07820

SLD:

T261_7397

SLC:	SL103_14825

SRW:	TUE45_01810(mlr_1)

SPAV:

Spa2297_05215

SCLF:

BB341_19095

SGS:	AVL59_25750

STSI:

A4E84_39335

SALW:

CP975_05110

SGM:	GCM10017557_69210

SCAD:

DN051_31695

SPHW:

NFX46_27495

SLS:	SLINC_8171

SNR:	SNOUR_35730

SALU:

DC74_1378

SALL:

SAZ_07405

SAUO:

BV401_07295

SSIA:

A7J05_30010

SPUN:

BFF78_40630

SGV:	B1H19_07005

SALF:

SMD44_01424

STRO:

STRMOE7_05000

SFK:

KY5_1140

SNZ:	DC008_04090

SGE:	DWG14_07333(dmg)

SLK:	SLUN_37375

SGD:	ELQ87_32780

SCYA:

EJ357_07090

SKA:	CP970_37115

SGAL:

CP966_05380

SPAD:

DVK44_29080

SFY:	GFH48_33555

SAQU:

EJC51_07185

SSEO:

D0Z67_03935

SRIM:

CP984_35535

SPHV:

F9278_44460

SPAC:

B1H29_30600

SCHA:

CP983_39600

SCOE:

CP976_02350

SSPB:

CP982_08295

SPLA:

CP981_03560

SBRO:

GQF42_41155

STUI:

GCM10017668_68780

SCYG:

S1361_07765(mlr1)

SDW:	K7C20_03430

SAUH:

SU9_002685

SROI:

IAG44_37905

SXN:	IAG42_07500

SHK:	J2N69_05045

SANU:

K7396_32050

SMAO:

CAG99_15110

SGOB:

test1122_25615

SINE:

KI385_05965

SLF:	JEQ17_38800

SDEC:

L3078_42340

SAOV:

G3H79_31790

SDUR:

M4V62_35110

SAKB:

K1J60_01500

SNIG:

HEK616_25470

SCAE:

IHE65_02350

STUB:

MMF93_29570

SSPN:

LXH13_05225

SCIR:

STRCI_001444

SLON:

LGI35_10155

STUD:

STRTU_006579

SANT:

QR300_37170

SYUN:

MOV08_36310

SJN:	RI060_39030

SCYN:

N8I84_06770

SHAU:

K9S39_37535

SKG:	KJK29_32385

SFP:	QUY26_34160

SINN:

ABB07_36380

KBU:	Q4V64_08695

SARM:

DVA86_26390

SPRF:

K4G22_08390

SLUO:

PS467_06675

STPB:

QR97_30150

SRPA:

HEP86_05075

SMIB:

SMIR_34410

SCAJ:

O1G22_39815

AGLA:

OIE69_08215

ART:

Arth_3718

ARR:	ARUE_c04530

ARH:	AHiyo8_21700

ARY:	ATC04_01645

ARU:	ASPU41_10500

ARQ:	BWQ92_12560 BWQ92_12630

ARN:	CGK93_02470

ARX:	ARZXY2_3454

ACRY:

AC20117_06445 AC20117_10905

ARTH:

C3B78_00870

ARTP:

E5206_00090

ASUF:

MNQ99_15645

ARTY:

AHiyo4_00980 AHiyo4_02080

AWW:	G8758_07240

AOD:	Q8Z05_05540 Q8Z05_05605

AAU:

AAur_0484

PUE:	FV140_02605

PNV:	JMY29_02220

PAYJ:

HMI59_18885

PAGO:

IRJ34_02120

ARZ:	AUT26_02035

ACH:	Achl_3463 Achl_3469

APN:	Asphe3_02290

PSUL:

AU252_09435

PSNI:

NIBR502771_07425 NIBR502771_07445

PSEY:

GU243_15190

PDEL:

JCQ34_17175 JCQ34_17195

POK:	SMD14_01120

PSLS:

KTR40_00965

GAR:	AOZ07_16460

GCR:	GcLGCM259_2841

GLU:	F0M17_15520

GMI:	NMP99_16080

GMY:

XH9_09775

GNC:	QQS42_16130

GLM:	N6V40_04030

KFV:	AS188_03570

KRS:	EQG70_16575

SATK:

SA2016_0189 SA2016_3446

CCYC:

SCMU_10070 SCMU_34040

NAE:	BHE16_09345

CMUA:

P8192_05095

PAEY:

KUF55_01330

BSAU:

DWV08_05330

BLY:	A2T55_01520

BLIN:

BLSMQ_0189

BCAU:

I6G59_04785

BSPO:

L1F31_01030

NBI:	SHK19_05095

NGV:	CDO52_10660

STRR:

EKD16_17065(mlr2)

SAIU:

J4H86_06200

SRO:

Sros_3855

NOA:	BKM31_33565

NOW:	GBF35_11145

NCX:	Nocox_10415(mlr2)

NGN:	LCN96_11830

NFS:	OIE67_35180

MHAI:

OHB01_19070

FRI:	FraEuI1c_1272

NAK:	EH165_03045

NAKA:

H7F38_07085

GOB:

Gobs_2879

BSD:	BLASA_3541

BBRG:

U6N30_19665

MMAR:

MODMU_2885

SEN:	SACE_3149 SACE_4496 SACE_5616

SACG:

FDZ84_03475 FDZ84_32380

SVI:	Svir_18850

AMD:

AMED_7548

AMN:

RAM_38785

AMM:

AMES_7436

AMZ:

B737_7436

AOI:

AORI_6809

AJA:	AJAP_05070

AMQ:	AMETH_4486

AMYC:

CU254_17875

AMYB:

BKN51_27315

AAB:	A4R43_09625

AMYY:

YIM_25575(mlr4)

AORI:

SD37_06610

AACD:

LWP59_25030

APRT:

MUY14_46680

AROO:

NQK81_31955

ATHM:

L1857_25525

ARHD:

VSH64_11885

AMOG:

QRX60_21915

ACOR:

LCL61_13575

PSEA:

WY02_22370

PSEE:

FRP1_20290

PSEQ:

AD006_27705

PECQ:

AD017_07300

PHH:	AFB00_18135

PAUT:

Pdca_14210

PBRO:

HOP40_33600

PPEL:

H6H00_30545

PSDS:

K1T35_07060 K1T35_26930

KAL:

KALB_3171

KUT:	JJ691_09270 JJ691_34640

KPHY:

AOZ06_00305

LED:	BBK82_00765

AHM:	TL08_06780

ACTI:

UA75_06595

ACAD:

UA74_06595

AHG:	AHOG_06315(mlr)

PMAD:

BAY61_26185

SACE:

GIY23_13960 GIY23_15620

CROS:

N8J89_23390

ACTY:

OG774_13590

UME:	RM788_37525

AMS:	AMIS_66490

ACTN:

L083_4087

ACTR:

Asp14428_12640

ACTL:

L3i22_092120

ASIC:

Q0Z83_061200 Q0Z83_103280

ACTE:

ACTI_07060 ACTI_54970

AFS:

AFR_18435

AEY:	CDG81_08445

RXY:

Rxyl_0762

RRD:	RradSPS_0628

RUB:	GBA63_03815

RMAR:

GBA65_09885

CAP:	CLDAP_16610

MICA:

P0L94_18370

FLC:	KJS93_18725

HALR:

EFA46_013930

HJE:	HacjB3_10225

HDO:	MUK72_06290

HAXY:

NGM07_13820

HMA:	pNG7370(gcvT1)

HHI:	HAH_5196(gcvT4)

HHN:	HISP_19155

HAB:	SG26_17100

HTA:	BVU17_16770

HALJ:

G9465_20185

HSIN:

KDQ40_19490

HAAD:

MW046_16085

HWA:	HQ_1761A(dmg2)

HWC:	Hqrw_1891(dmg2)

HVO:	HVO_B0014(dmg1)

HALE:

G3A49_07115

HLN:	SVXHx_3458(dmg)

HGI:	ABY42_15525

HAQ:	DU484_17270

HAJ:	DU500_00310

HAER:

DU502_15680

HRA:	EI982_15310

HLM:	DV707_15295

HALB:

EKH57_15480

HALU:

HUG12_01570

HTU:

Htur_4706

HSAL:

JMJ58_24020

HXA:	Halxa_1398

NVR:	FEJ81_00105

NAY:	HYG81_10490

NOU:	Natoc_3307

NAS:	GCU68_06365

» show all

Reference

Authors

Mori N, Kawakami B, Tani Y, Yamada H.

Title

Purification and properties of dimethylglycine oxidase from Cylindrocarpon didymum M-1.

Journal

Agric Biol Chem 44:1383-1389 (1980)

Reference

2 [PMID:11422368]

Authors

Meskys R, Harris RJ, Casaite V, Basran J, Scrutton NS

Title

Organization of the genes involved in dimethylglycine and sarcosine degradation in Arthrobacter spp.: implications for glycine betaine catabolism.

Journal

Eur J Biochem 268:3390-8 (2001)
DOI:10.1046/j.1432-1327.2001.02239.x

Sequence

[ag:AAK16482]

Reference

3 [PMID:11926836]

Authors

Basran J, Bhanji N, Basran A, Nietlispach D, Mistry S, Meskys R, Scrutton NS.

Title

Mechanistic aspects of the covalent flavoprotein dimethylglycine oxidase of Arthrobacter globiformis studied by stopped-flow spectrophotometry.

Journal

Biochemistry 41:4733-43 (2002)
DOI:10.1021/bi025519h

Sequence

[ag:AAK16482]

Reference

4 [PMID:12912903]

Authors

Leys D, Basran J, Scrutton NS.

Title

Channelling and formation of 'active' formaldehyde in dimethylglycine oxidase.

Journal

EMBO J 22:4038-48 (2003)
DOI:10.1093/emboj/cdg395

Sequence

[ag:AAK16482]

Reference

5 [PMID:16964976]

Authors

Basran J, Fullerton S, Leys D, Scrutton NS.

Title

Mechanism of FAD reduction and role of active site residues His-225 and Tyr-259 in Arthrobacter globiformis dimethylglycine oxidase: analysis of mutant structure and catalytic function.

Journal

Biochemistry 45:11151-61 (2006)
DOI:10.1021/bi061094d

Reference

6 [PMID:19369258]

Authors

Tralau T, Lafite P, Levy C, Combe JP, Scrutton NS, Leys D.

Title

An internal reaction chamber in dimethylglycine oxidase provides efficient protection from exposure to toxic formaldehyde.

Journal

J Biol Chem 284:17826-34 (2009)
DOI:10.1074/jbc.M109.006262

Reference

7 [PMID:21188587]

Authors

Casaite V, Poviloniene S, Meskiene R, Rutkiene R, Meskys R.

Title

Studies of dimethylglycine oxidase isoenzymes in Arthrobacter globiformis cells.

Journal

Curr Microbiol 62:1267-73 (2011)
DOI:10.1007/s00284-010-9852-6

Other DBs

ExplorEnz - The Enzyme Database:

1.5.3.10

IUBMB Enzyme Nomenclature:

1.5.3.10

ExPASy - ENZYME nomenclature database:

1.5.3.10

BRENDA, the Enzyme Database:

1.5.3.10

CAS:	37256-30-7

LinkDB

DBGET integrated database retrieval system