KEGG   ENZYME: 1.5.7.1
Entry
EC 1.5.7.1                  Enzyme                                 
Name
methylenetetrahydrofolate reductase (ferredoxin);
5,10-methylenetetrahydrofolate reductase
Class
Oxidoreductases;
Acting on the CH-NH group of donors;
With an iron-sulfur protein as acceptor
Sysname
5-methyltetrahydrofolate:ferredoxin oxidoreductase
Reaction(IUBMB)
5-methyltetrahydrofolate + 2 oxidized ferredoxin = 5,10-methylenetetrahydrofolate + 2 reduced ferredoxin + 2 H+ [RN:R01217]
Reaction(KEGG)
R01217
Substrate
5-methyltetrahydrofolate [CPD:C00440];
oxidized ferredoxin [CPD:C00139]
Product
5,10-methylenetetrahydrofolate [CPD:C00143];
reduced ferredoxin [CPD:C00138];
H+ [CPD:C00080]
Comment
An iron-sulfur flavoprotein that also contains zinc. The enzyme from Clostridium formicoaceticum catalyses the reduction of methylene blue, menadione, benzyl viologen, rubredoxin or FAD with 5-methyltetrahydrofolate and the oxidation of reduced ferredoxin or FADH2 with 5,10-methylenetetrahydrofolate. However, unlike EC 1.5.1.53, methylenetetrahydrofolate reductase (NADPH); EC 1.5.1.54, methylenetetrahydrofolate reductase (NADH); or EC 1.5.1.20, methylenetetrahydrofolate reductase [NAD(P)H], there is no activity with either NADH or NADP+.
History
EC 1.5.7.1 created 2005, modified 2021
Pathway
ec00670  One carbon pool by folate
Reference
1  [PMID:6381490]
  Authors
Clark JE, Ljungdahl LG.
  Title
Purification and properties of 5,10-methylenetetrahydrofolate reductase, an iron-sulfur flavoprotein from Clostridium formicoaceticum.
  Journal
J Biol Chem 259:10845-9 (1984)
Other DBs
ExplorEnz - The Enzyme Database: 1.5.7.1
IUBMB Enzyme Nomenclature: 1.5.7.1
ExPASy - ENZYME nomenclature database: 1.5.7.1
BRENDA, the Enzyme Database: 1.5.7.1
LinkDB

KEGG   REACTION: R01217
Entry
R01217                      Reaction                               
Name
5-methyltetrahydrofolate:ferredoxin oxidoreductase
Definition
5,10-Methylenetetrahydrofolate + 2 Reduced ferredoxin + 2 H+ <=> 5-Methyltetrahydrofolate + 2 Oxidized ferredoxin
Equation
C00143 + 2 C00138 + 2 C00080 <=> C00440 + 2 C00139
Reaction class
RC00081  C00143_C00440
Enzyme
1.5.7.1
Pathway
rn00670  One carbon pool by folate
rn01100  Metabolic pathways
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.5  Acting on the CH-NH group of donors
   1.5.7  With an iron-sulfur protein as acceptor
    1.5.7.1
     R01217  5,10-Methylenetetrahydrofolate + 2 Reduced ferredoxin + 2 H+ <=> 5-Methyltetrahydrofolate + 2 Oxidized ferredoxin
Other DBs
RHEA: 14232
LinkDB

DBGET integrated database retrieval system