KEGG   ENZYME: 1.7.1.14
Entry
EC 1.7.1.14                 Enzyme                                 
Name
nitric oxide reductase [NAD(P)+, nitrous oxide-forming];
fungal nitric oxide reductase;
cytochrome P450nor;
NOR (ambiguous)
Class
Oxidoreductases;
Acting on other nitrogenous compounds as donors;
With NAD+ or NADP+ as acceptor
Sysname
nitrous oxide:NAD(P) oxidoreductase
Reaction(IUBMB)
N2O + NAD(P)+ + H2O = 2 NO + NAD(P)H + H+ [RN:R02492 R09446]
Reaction(KEGG)
R02492 R09446;
(other) R09808 R09809
Substrate
N2O [CPD:C00887];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Product
NO [CPD:C00533];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
A heme-thiolate protein (P-450). The enzyme from Fusarium oxysporum utilizes only NADH, but the isozyme from Trichosporon cutaneum utilizes both NADH and NADPH. The electron transfer from NAD(P)H to heme occurs directly, not requiring flavin or other redox cofactors.
History
EC 1.7.1.14 created 2011
Pathway
ec00910  Nitrogen metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K15877  fungal nitric oxide reductase
Genes
CNS116350878
CRECHLRE_01g007950v5
CVRCHLNCDRAFT_51513
NCRNCU06137
NTENEUTE1DRAFT117740(NEUTE1DRAFT_117740)
SMPSMAC_06378
PANPODANSg1411
TTTTHITE_2117644
FGRFGSG_11585
FVRFVEG_10773
FOXFOXG_12350
NHENECHADRAFT_41997 NECHADRAFT_89386 NECHADRAFT_97205
MAWMAC_00270 MAC_05732
MAJMAA_08207 MAA_08689
PLJVFPFJ_05955
CFJCFIO01_11821
SAPOSAPIO_CDS4621
SSLSS1G_00406
MBEMBM_04680
PSCOLY89DRAFT_614822
GLZGLAREA_09633
AORAO090166000070
ANGANI_1_2092064(An07g08930)
AFVAFLA_011427
UREUREG_06342
PBLPAAG_02515
PBNPADG_05660
ABEARB_00546
TVETRV_00378
AJEHCAG_02570
BGHBDBG_08235
BSCCOCSADRAFT_355241
TASAA1Q1_03733
 » show all
Reference
1  [PMID:2040619]
  Authors
Shoun H, Tanimoto T
  Title
Denitrification by the fungus Fusarium oxysporum and involvement of cytochrome P-450 in the respiratory nitrite reduction.
  Journal
J Biol Chem 266:11078-82 (1991)
  Sequence
Reference
2  [PMID:7829493]
  Authors
Shiro Y, Fujii M, Iizuka T, Adachi S, Tsukamoto K, Nakahara K, Shoun H
  Title
Spectroscopic and kinetic studies on reaction of cytochrome P450nor with nitric oxide. Implication for its nitric oxide reduction mechanism.
  Journal
J Biol Chem 270:1617-23 (1995)
DOI:10.1074/jbc.270.4.1617
  Sequence
Reference
3  [PMID:12105197]
  Authors
Zhang L, Kudo T, Takaya N, Shoun H
  Title
The B' helix determines cytochrome P450nor specificity for the electron donors NADH and NADPH.
  Journal
J Biol Chem 277:33842-7 (2002)
DOI:10.1074/jbc.M203923200
  Sequence
Reference
4  [PMID:15313618]
  Authors
Oshima R, Fushinobu S, Su F, Zhang L, Takaya N, Shoun H
  Title
Structural evidence for direct hydride transfer from NADH to cytochrome P450nor.
  Journal
J Mol Biol 342:207-17 (2004)
DOI:10.1016/j.jmb.2004.07.009
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.7.1.14
IUBMB Enzyme Nomenclature: 1.7.1.14
ExPASy - ENZYME nomenclature database: 1.7.1.14
BRENDA, the Enzyme Database: 1.7.1.14
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