KEGG   ENZYME: 2.1.1.246
Entry
EC 2.1.1.246                Enzyme                                 

Name
[methyl-Co(III) methanol-specific corrinoid protein]---coenzyme M methyltransferase;
methyltransferase 2 (ambiguous);
mtaA (gene name)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
methylated methanol-specific corrinoid protein:CoM methyltransferase
Reaction(IUBMB)
a [methyl-Co(III) methanol-specific corrinoid protein] + CoM = methyl-CoM + a [Co(I) methanol-specific corrinoid protein] [RN:R10000]
Reaction(KEGG)
R10000;
(other) R09098
Substrate
[methyl-Co(III) methanol-specific corrinoid protein];
CoM [CPD:C03576]
Product
methyl-CoM [CPD:C03920];
[Co(I) methanol-specific corrinoid protein]
Comment
The enzyme, which is involved in methanogenesis from methanol, catalyses the transfer of a methyl group from a corrinoid protein (see EC 2.1.1.90, methanol---corrinoid protein Co-methyltransferase), where it is bound to the cobalt cofactor, to CoM, forming the substrate for EC 2.8.4.1, coenzyme-B sulfoethylthiotransferase, the enzyme that catalyses the final step in methanogenesis. Free methylcob(I)alamin can substitute for the corrinoid protein in vitro [5].
History
EC 2.1.1.246 created 2012
Pathway
ec00680  Methane metabolism
ec01120  Microbial metabolism in diverse environments
Orthology
K14080  [methyl-Co(III) methanol-specific corrinoid protein]:coenzyme M methyltransferase
Genes
DEO: CAY53_02410
DTI: Desti_0877 Desti_2036 Desti_3217 Desti_5361
DKU: Desku_0050 Desku_0055 Desku_0060
TJR: TherJR_0470 TherJR_0979 TherJR_0982
VGU: HYG85_23900
SRI: SELR_27690(mtbA)
MST: Msp_0112(mtaA2) Msp_0182(mtaA4) Msp_0761(mtaA1) Msp_0774(mtaA3)
METB: AW729_02405 AW729_06195 AW729_10490
MSI: Msm_0518
MEL: Metbo_1025
MCUB: MCBB_1152(mtaA)
MAC: MA_0779(cmtM) MA_1615(cmtM) MA_2163(cmtM) MA_3613(cmtM) MA_4379(mtaA) MA_4559(cmtM)
MMA: MM_0176(mtaA) MM_0505 MM_1070(mtaA) MM_1932(mtbA)
MBU: Mbur_0452(mtaA) Mbur_0808 Mbur_1005(mtaA)
MEAR: Mpt1_c01960(mtAA1)
 » show all
Reference
1  [PMID:8702528]
  Authors
LeClerc GM, Grahame DA
  Title
Methylcobamide:coenzyme M methyltransferase isozymes from Methanosarcina barkeri. Physicochemical characterization, cloning, sequence analysis, and heterologous gene expression.
  Journal
J Biol Chem 271:18725-31 (1996)
DOI:10.1074/jbc.271.31.18725
  Sequence
Reference
2  [PMID:8654414]
  Authors
Harms U, Thauer RK
  Title
Methylcobalamin: coenzyme M methyltransferase isoenzymes MtaA and MtbA from Methanosarcina barkeri. Cloning, sequencing and differential transcription of the encoding genes, and functional overexpression of the mtaA gene in Escherichia coli.
  Journal
Eur J Biochem 235:653-9 (1996)
DOI:10.1111/j.1432-1033.1996.00653.x
  Sequence
Reference
3  [PMID:9363780]
  Authors
Sauer K, Thauer RK
  Title
Methanol:coenzyme M methyltransferase from Methanosarcina barkeri. Zinc dependence and thermodynamics of the methanol:cob(I)alamin methyltransferase reaction.
  Journal
Eur J Biochem 249:280-5 (1997)
DOI:10.1111/j.1432-1033.1997.t01-1-00280.x
Reference
4  [PMID:9057830]
  Authors
Sauer K, Harms U, Thauer RK
  Title
Methanol:coenzyme M methyltransferase from Methanosarcina barkeri. Purification, properties and encoding genes of the corrinoid protein MT1.
  Journal
Eur J Biochem 243:670-7 (1997)
DOI:10.1111/j.1432-1033.1997.t01-1-00670.x
Reference
5  [PMID:10215883]
  Authors
Sauer K, Thauer RK
  Title
Methanol:coenzyme M methyltransferase from Methanosarcina barkeri -- substitution of the corrinoid harbouring subunit MtaC by free cob(I)alamin.
  Journal
Eur J Biochem 261:674-81 (1999)
DOI:10.1046/j.1432-1327.1999.00355.x
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.246
IUBMB Enzyme Nomenclature: 2.1.1.246
ExPASy - ENZYME nomenclature database: 2.1.1.246
BRENDA, the Enzyme Database: 2.1.1.246
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