KEGG ENZYME: 2.1.1.246

ENZYME: 2.1.1.246

Entry

EC 2.1.1.246                Enzyme

Name

[methyl-Co(III) methanol-specific corrinoid protein]---coenzyme M methyltransferase;
methyltransferase 2 (ambiguous);
mtaA (gene name)

Class

Transferases;
Transferring one-carbon groups;
Methyltransferases

Sysname

methylated methanol-specific corrinoid protein:CoM methyltransferase

Reaction(IUBMB)

a [methyl-Co(III) methanol-specific corrinoid protein] + CoM = methyl-CoM + a [Co(I) methanol-specific corrinoid protein] [RN:R10000]

Reaction(KEGG)

R10000;
(other) R09098

Substrate

[methyl-Co(III) methanol-specific corrinoid protein];
CoM [CPD:C03576]

Product

methyl-CoM [CPD:C03920];
[Co(I) methanol-specific corrinoid protein]

Comment

The enzyme, which is involved in methanogenesis from methanol, catalyses the transfer of a methyl group from a corrinoid protein (see EC 2.1.1.90, methanol---corrinoid protein Co-methyltransferase), where it is bound to the cobalt cofactor, to CoM, forming the substrate for EC 2.8.4.1, coenzyme-B sulfoethylthiotransferase, the enzyme that catalyses the final step in methanogenesis. Free methylcob(I)alamin can substitute for the corrinoid protein in vitro [5].

History

EC 2.1.1.246 created 2012

Pathway

ec00680

Methane metabolism

ec01120

Microbial metabolism in diverse environments

Orthology

K14080

[methyl-Co(III) methanol/glycine betaine-specific corrinoid protein]:coenzyme M methyltransferase

Genes

DEO:	CAY53_02410

DTI:	Desti_0877 Desti_2036 Desti_3217 Desti_5361

TAER:

GT409_09440

TVX:	QBE54_03685

PALM:

RBG61_11480

BIE:	RFF05_13435

DKU:	Desku_0050 Desku_0055 Desku_0060

FWA:	DCMF_12445 DCMF_12460

TJR:	TherJR_0470 TherJR_0979 TherJR_0982

ACAE:

HYG86_16410

BCOC:

BLCOC_16950

VGU:	HYG85_23900

DBC:	MFMK1_003229

AMEI:

LV469_06415

SRI:	SELR_27690(mtbA)

CALK:

HUE98_05430

MST:	Msp_0112(mtaA2) Msp_0182(mtaA4) Msp_0761(mtaA1) Msp_0774(mtaA3)

METB:

AW729_02405 AW729_06195 AW729_10490

MEIS:

PXD04_02420(mtaA) PXD04_04500 PXD04_04505 PXD04_04790 PXD04_08395

MSI:

Msm_0518

MARO:

MarbSA_04020

METV:

K4897_03100

MEL:	Metbo_1025

MEW:	MSWAN_1084

MCUB:

MCBB_1152(mtaA)

MEER:

NKF70_01290(mtaA)

FPL:	Ferp_1746 Ferp_1761

MBA:	Mbar_A1054 Mbar_A1688 Mbar_A3639

MBY:	MSBRM_2354 MSBRM_2991 MSBRM_3313

MBW:	MSBRW_1093 MSBRW_3045 MSBRW_3668

MBAR:

MSBR2_2320 MSBR2_2952 MSBR2_3267

MBAK:

MSBR3_1746 MSBR3_2381 MSBR3_3262

MAC:	MA_0779(cmtM) MA_1615(cmtM) MA_2163(cmtM) MA_3613(cmtM) MA_4379(mtaA) MA_4559(cmtM)

MMA:	MM_0176(mtaA) MM_0505 MM_1070(mtaA) MM_1932(mtbA)

MMAZ:

MmTuc01_0522 MmTuc01_1102 MmTuc01_2009

MMJ:	MSMAS_0231 MSMAS_0801 MSMAS_1125 MSMAS_2752

MMAC:

MSMAC_0237 MSMAC_0779 MSMAC_1671 MSMAC_2485

MVC:	MSVAZ_0217 MSVAZ_0373 MSVAZ_1364

MEK:	MSKOL_0211 MSKOL_0358 MSKOL_1307

MLS:	MSLAZ_0369 MSLAZ_1228 MSLAZ_1379 MSLAZ_2322 MSLAZ_2965

METM:

MSMTP_0087 MSMTP_0267 MSMTP_1139 MSMTP_1438 MSMTP_1905 MSMTP_1916 MSMTP_2416 MSMTP_2659

MEF:	MSWH1_0532 MSWH1_1021 MSWH1_1747 MSWH1_2472 MSWH1_2627

MEQ:	MSWHS_0547 MSWHS_1092 MSWHS_1568 MSWHS_2411 MSWHS_2572

MSJ:	MSSAC_0718 MSSAC_1597 MSSAC_1859 MSSAC_2001 MSSAC_3198 MSSAC_4064 MSSAC_4242

MSZ:	MSSIH_0662 MSSIH_1027 MSSIH_1186 MSSIH_2365 MSSIH_3600 MSSIH_3745

MSW:	MSSIT_0690 MSSIT_1074 MSSIT_1253 MSSIT_2316 MSSIT_2424 MSSIT_3653 MSSIT_3824

MTHR:

MSTHT_0082 MSTHT_1209 MSTHT_1754

MTHE:

MSTHC_0648 MSTHC_1535 MSTHC_2085

MHOR:

MSHOH_0293 MSHOH_0908 MSHOH_1347 MSHOH_1564 MSHOH_3370 MSHOH_3435

MFZ:	AOB57_002025 AOB57_007965 AOB57_010270

MHAB:

RSJ42_00660(mtaA) RSJ42_03615(mtaA) RSJ42_11610(mtaA) RSJ42_14700(mtaA)

METW:

AAY43_02680 AAY43_07435 AAY43_07610

MBU:	Mbur_0452(mtaA) Mbur_0808 Mbur_1005(mtaA)

MMET:

MCMEM_0940 MCMEM_1137 MCMEM_2051

MELO:

J7W08_02985 J7W08_04245

MMH:	Mmah_0008 Mmah_0518 Mmah_0901 Mmah_0970 Mmah_1478

MHAZ:

BHR79_00830 BHR79_03425 BHR79_08720 BHR79_09050

MPOT:

BKM01_04990 BKM01_07580 BKM01_08935 BKM01_10485

MEV:	Metev_0012 Metev_0013 Metev_0942

MZH:	Mzhil_0011 Mzhil_0619 Mzhil_1798 Mzhil_1957

MPY:	Mpsy_0341 Mpsy_0911

MZI:	HWN40_04040 HWN40_06230 HWN40_07880

MMAV:

RE476_03530(mtaA) RE476_06210(mtaA) RE476_06905(mtaA)

MSEB:

RE474_02320(mtaA) RE474_11785(mtaA) RE474_13240(mtaA)

MHZ:	Metho_0761 Metho_0776 Metho_1260

MEHF:

MmiHf6_01690(hemE_2) MmiHf6_11380(hemE_6)

MEES:

MmiEs2_08220(hemE_2) MmiEs2_14070(hemE_5)

MEAM:

MU439_00860 MU439_01115 MU439_01140(mtaA) MU439_02360 MU439_05065(mtaA)

MEAE:

QEN48_01425(mtaA) QEN48_01460 QEN48_03630 QEN48_05100(mtaA) QEN48_07910

TAR:	TALC_00463 TALC_00742

MAX:	MMALV_03890 MMALV_05210 MMALV_09620

MER:	MMINT_07210 MMINT_13560 MMINT_15760

MEAR:

Mpt1_c01960(mtAA1)

MEUZ:

KRP56_02065 KRP56_04335

MARC:

AR505_1035 AR505_1063 AR505_1404 AR505_1575

» show all

Reference

1 [PMID:8702528]

Authors

LeClerc GM, Grahame DA

Title

Methylcobamide:coenzyme M methyltransferase isozymes from Methanosarcina barkeri. Physicochemical characterization, cloning, sequence analysis, and heterologous gene expression.

Journal

J Biol Chem 271:18725-31 (1996)
DOI:10.1074/jbc.271.31.18725

Sequence

[mba:Mbar_A1054]

Reference

2 [PMID:8654414]

Authors

Harms U, Thauer RK

Title

Methylcobalamin: coenzyme M methyltransferase isoenzymes MtaA and MtbA from Methanosarcina barkeri. Cloning, sequencing and differential transcription of the encoding genes, and functional overexpression of the mtaA gene in Escherichia coli.

Journal

Eur J Biochem 235:653-9 (1996)
DOI:10.1111/j.1432-1033.1996.00653.x

Sequence

[mba:Mbar_A1054]

Reference

3 [PMID:9363780]

Authors

Sauer K, Thauer RK

Title

Methanol:coenzyme M methyltransferase from Methanosarcina barkeri. Zinc dependence and thermodynamics of the methanol:cob(I)alamin methyltransferase reaction.

Journal

Eur J Biochem 249:280-5 (1997)
DOI:10.1111/j.1432-1033.1997.t01-1-00280.x

Reference

4 [PMID:9057830]

Authors

Sauer K, Harms U, Thauer RK

Title

Methanol:coenzyme M methyltransferase from Methanosarcina barkeri. Purification, properties and encoding genes of the corrinoid protein MT1.

Journal

Eur J Biochem 243:670-7 (1997)
DOI:10.1111/j.1432-1033.1997.t01-1-00670.x

Reference

5 [PMID:10215883]