KEGG   ENZYME: 2.1.1.251
Entry
EC 2.1.1.251                Enzyme                                 
Name
methylated-thiol---coenzyme M methyltransferase;
mtsA (gene name)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
methylated-thiol:CoM methyltransferase
Reaction(IUBMB)
methanethiol + CoM = methyl-CoM + hydrogen sulfide (overall reaction) [RN:R09997];
(1a) methanethiol + a [Co(I) methylated-thiol-specific corrinoid protein] = a [methyl-Co(III) methylated-thiol-specific corrinoid protein] + hydrogen sulfide [RN:R10001];
(1b) a [methyl-Co(III) methylated-thiol-specific corrinoid protein] + CoM = methyl-CoM + a [Co(I) methylated-thiol-specific corrinoid protein] [RN:R10000]
Reaction(KEGG)
Substrate
methanethiol [CPD:C00409];
CoM [CPD:C03576];
[Co(I) methylated-thiol-specific corrinoid protein];
[methyl-Co(III) methylated-thiol-specific corrinoid protein]
Product
methyl-CoM [CPD:C03920];
hydrogen sulfide [CPD:C00283];
[methyl-Co(III) methylated-thiol-specific corrinoid protein];
[Co(I) methylated-thiol-specific corrinoid protein]
Comment
The enzyme, which is involved in methanogenesis from methylated thiols, such as methane thiol, dimethyl sulfide, and 3-(methylsulfanyl)propanoate, catalyses two successive steps - the transfer of a methyl group from the substrate to the cobalt cofactor of a methylated-thiol-specific corrinoid protein (MtsB), and the subsequent transfer of the methyl group from the corrinoid protein to CoM. With most other methanogenesis substrates this process is carried out by two different enzymes (for example, EC 2.1.1.90, methanol---corrinoid protein Co-methyltransferase, and EC 2.1.1.246, [methyl-Co(III) methanol-specific corrinoid protein]---coenzyme M methyltransferase). The cobalt is oxidized during methylation from the Co(I) state to the Co(III) state, and is reduced back to the Co(I) form during demethylation.
History
EC 2.1.1.251 created 2012
Pathway
ec00920  Sulfur metabolism
ec01100  Metabolic pathways
Orthology
K16954  methylthiol:coenzyme M methyltransferase
Genes
AWICHL1_003026
MBAMbar_A3585
MBYMSBRM_1542
MBWMSBRW_1148
MBARMSBR2_0865
MBAKMSBR3_1223
MMAMM_2427(mtsA)
MMAZMmTuc01_2485
MMJMSMAS_2273
MMACMSMAC_2181
MVCMSVAZ_1681
MEKMSKOL_1639
MLSMSLAZ_0851
HAKZJ0X25_19555
MAXMMALV_13470
MARCAR505_1066
 » show all
Reference
1  [PMID:8932317]
  Authors
Paul L, Krzycki JA
  Title
Sequence and transcript analysis of a novel Methanosarcina barkeri methyltransferase II homolog and its associated corrinoid protein homologous to methionine synthase.
  Journal
J Bacteriol 178:6599-607 (1996)
DOI:10.1128/JB.178.22.6599-6607.1996
  Sequence
Reference
2  [PMID:9371433]
  Authors
Tallant TC, Krzycki JA
  Title
Methylthiol:coenzyme M methyltransferase from Methanosarcina barkeri, an enzyme of methanogenesis from dimethylsulfide and methylmercaptopropionate.
  Journal
J Bacteriol 179:6902-11 (1997)
DOI:10.1128/JB.179.22.6902-6911.1997
  Sequence
Reference
3  [PMID:11073950]
  Authors
Tallant TC, Paul L, Krzycki JA
  Title
The MtsA subunit of the methylthiol:coenzyme M methyltransferase of Methanosarcina barkeri catalyses both half-reactions of corrinoid-dependent dimethylsulfide: coenzyme M methyl transfer.
  Journal
J Biol Chem 276:4485-93 (2001)
DOI:10.1074/jbc.M007514200
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.251
IUBMB Enzyme Nomenclature: 2.1.1.251
ExPASy - ENZYME nomenclature database: 2.1.1.251
BRENDA, the Enzyme Database: 2.1.1.251
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