KEGG ENZYME: 2.1.1.263

ENZYME: 2.1.1.263

Entry

EC 2.1.1.263                Enzyme

Name

botryococcene C-methyltransferase;
TMT-3

Class

Transferases;
Transferring one-carbon groups;
Methyltransferases

Sysname

S-adenosyl-L-methionine:botryococcene C-methyltransferase

Reaction(IUBMB)

2 S-adenosyl-L-methionine + C30 botryococcene = 2 S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene (overall reaction) [RN:R10199];
(1a) S-adenosyl-L-methionine + C30 botryococcene = S-adenosyl-L-homocysteine + 3-methyl-1,2-didehydro-2,3-dihydrobotryococcene [RN:R10195];
(1b) S-adenosyl-L-methionine + 3-methyl-1,2-didehydro-2,3-dihydrobotryococcene = S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene [RN:R10196];
(2a) S-adenosyl-L-methionine + C30 botryococcene = S-adenosyl-L-homocysteine + 20-methyl-21,22-didehydro-20,21-dihydrobotryococcene [RN:R10197];
(2b) S-adenosyl-L-methionine + 20-methyl-21,22-didehydro-20,21-dihydrobotryococcene = S-adenosyl-L-homocysteine + 3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene [RN:R10198]

Reaction(KEGG)

R10195 R10196 R10197 R10198 R10199

Substrate

S-adenosyl-L-methionine [CPD:C00019];
C30 botryococcene [CPD:C20432];
3-methyl-1,2-didehydro-2,3-dihydrobotryococcene [CPD:C20433];
20-methyl-21,22-didehydro-20,21-dihydrobotryococcene [CPD:C20434]

Product

S-adenosyl-L-homocysteine [CPD:C00021];
3,20-dimethyl-1,2,21,22-tetradehydro-2,3,20,21-tetrahydrobotryococcene [CPD:C20435];
3-methyl-1,2-didehydro-2,3-dihydrobotryococcene [CPD:C20433];
20-methyl-21,22-didehydro-20,21-dihydrobotryococcene [CPD:C20434]

Comment

Isolated from the green alga Botryococcus braunii BOT22. Shows a very weak activity with squalene.

History

EC 2.1.1.263 created 2012

Orthology

K21542

botryococcene C-methyltransferase

Reference

1 [PMID:22241476]

Authors

Niehaus TD, Kinison S, Okada S, Yeo YS, Bell SA, Cui P, Devarenne TP, Chappell J

Title