KEGG   ENZYME: 2.4.2.54
Entry
EC 2.4.2.54                 Enzyme                                 
Name
beta-ribofuranosylphenol 5'-phosphate synthase;
beta-RFAP synthase (incorrect);
beta-RFA-P synthase (incorrect);
AF2089 (gene name);
MJ1427 (gene name);
beta-ribofuranosylhydroxybenzene 5'-phosphate synthase;
4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate synthase (incorrect);
beta-ribofuranosylaminobenzene 5'-phosphate synthase (incorrect);
5-phospho-alpha-D-ribose 1-diphosphate:4-aminobenzoate 5-phospho-beta-D-ribofuranosyltransferase (decarboxylating) (incorrect)
Class
Transferases;
Glycosyltransferases;
Pentosyltransferases
Sysname
5-phospho-alpha-D-ribose-1-diphosphate:4-hydroxybenzoate 5-phospho-beta-D-ribofuranosyltransferase (decarboxylating)
Reaction(IUBMB)
5-phospho-alpha-D-ribose 1-diphosphate + 4-hydroxybenzoate = 4-(beta-D-ribofuranosyl)phenol 5'-phosphate + CO2 + diphosphate [RN:R11102]
Reaction(KEGG)
R11102;
(other) R10337
Substrate
5-phospho-alpha-D-ribose 1-diphosphate [CPD:C00119];
4-hydroxybenzoate [CPD:C00156]
Product
4-(beta-D-ribofuranosyl)phenol 5'-phosphate [CPD:C21107];
CO2 [CPD:C00011];
diphosphate [CPD:C00013]
Comment
The enzyme is involved in biosynthesis of tetrahydromethanopterin in archaea. It can utilize both 4-hydroxybenzoate and 4-aminobenzoate as substrates, but only the former is known to be produced by methanogenic archaea [4]. The activity is dependent on Mg2+ or Mn2+ [1].
History
EC 2.4.2.54 created 2013, modified 2014, modified 2015
Pathway
ec00790  Folate biosynthesis
Orthology
K06984  beta-ribofuranosylaminobenzene 5'-phosphate synthase
Genes
SFRSfri_3142
SBJCF168_02950
PSEOOM33_01310
MCAMCA2861
METUGNH96_03540
MMTMetme_1363 Metme_2289
MDNJT25_019570
MDHAYM39_07270 AYM39_11925
MKOMKLM6_2459
METLU737_09895
MPADKEF85_11140
MELLIVG45_02775
MAHMEALZ_2423(mptG)
MBUREQU24_09135
MPSYCEK71_02825
MMAIsS8_3396
MMOBF6R98_02835
MEINmethR_P2330
MOZMoryE10_28910
MISZMishRS11D_40040
MESLKKZ03_09065
MEJQ7A_615
MECQ7C_2201
BLEPAL038_03750
NHLNhal_3873
NWRE3U44_04690
TSYTHSYN_31795
NIKF5I99_01510
SEDSAAY24_01635
COXE0W60_31915
BYIBYI23_B014490
BUEBRPE67_BCDS01480
BPHBphy_5949 Bphy_6481
BCAIK788_0001553 K788_0008555
PARABTO02_24140
PHSC2L64_21830 C2L64_38310
PTERC2L65_26140 C2L65_34305
BUOBRPE64_BCDS01620
AFAUZ73_08470
VAPVapar_3085
VPDVAPA_1c31490
MPTMpe_A2606
METPC1M51_15110
RGERGE_40220(mptG)
LCHLcho_3153
AONDEH84_19010
METRBSY238_3295
MFAMfla_1661
MMBMmol_1347
MEHM301_1557
MEIMsip34_1505
MEPMPQ_1568
MBACBN1209_0744
MEUACJ67_08185
MPAUZMTM_11810
SPLBSFPGR_26010
FLEKI610_12870
AZAAZKH_p0276(mptG)
AZDCDA09_11300
THUAC731_004835
THKCCZ27_13260
DDZDSYM_25660
SULRB649_07545
DOVDSCO28_33840
DMMdnm_005860
MCIMesci_4257
MESWA9K65_023605
MESMEJ066_29705
MESMeso_2350
PHTBLM14_23775
RGARGR602_PC01565
NHANham_0792
XAUXaut_3535
XDIEZH22_18280
SNOSnov_0753
STARG3545_09765
LNEFZC33_09320
ANCGBB76_00765
APRAG3A50_16510
APOLK9D25_20175
MEAMex_1p1760(mptG)
MDIMETDI2512(mptG)
MEXMext_1828
MCHMchl_2163
MPOMpop_1779
MZAB2G69_16800
MRDMrad2831_0527
METM446_5777
MNOMnod_6001
MORMOC_0682
METAY590_08755
MAQUMaq22A_c16455(mptG)
MPHYMCBMB27_00104
MEEDA075_05690
METDC0214_10510
METXA3862_28315
METSDK389_20640
METIDK427_18430
MMESMMSR116_16675
MTEADK419_10780
MINDmvi_45880
MOGMMB17_20040
MTADM6G65_13680
MSLMsil_2384
MTUNMTUNDRAET4_2720
MLGCWB41_06030
HDNHden_1480
HDTHYPDE_31243
HMCHYPMC_1630(mptG)
HNIW911_13160
FILBN1229_v1_0589(mptG)
FIYBN1229_v1_0591(mptG)
MCGGL4_1789
METGHT051_10165
MSCBN69_2822
MBRYB1812_13625
MROSEHO51_01955
MHEYH2LOC_017505
MPARF7D14_03130
MTWCQW49_10280
CMETK6K41_25490
PLEOOHA_1_01038
HDIHDIA_2309
AALAIGS74_05795
AALMLUX29_06550
AUZSa4125_38500
AUBLXB15_00995
GBEGbCGDNIH1_0776
GBHGbCGDNIH2_0776
GBCGbCGDNIH3_0776
GBSGbCGDNIH4_0776
ALIAZOLI_p10689 AZOLI_p10892
ABQABAZ39_25455
ABFAMK58_22345
ATIAL072_27065
AZTTSH58p_01190
AZMDM194_17090
AOZHUE56_08935
SLPSlip_0785
PTHPTH_0622
DAEDtox_2738
FWADCMF_03860
DGIDesgi_4130
HASHalsa_0938
NTPCRH09_08300
SLIAHA039_21050
SAQSare_0579
RBARB3940
PSLPsta_2630
PIRVN12_20990
RULUC8_36510
MFFMFFC18_33300
ROLCA51_37630
AHELQ31a_33700
LCREPla8534_63820
AAGGETAA8_20720
BVOPan97_26050
RLCK227x_12670
LPAVPLANPX_2715
AMUCPan181_13200
PNDPla175_32920
AMOBHG15A2_24450
PLMPlim_2423
PEHSpb1_32070
PBSPlabr_3503
PLSVT03_17505
PLHVT85_12910
FMRFuma_00726
GMRGmarT_11040
GIMF1728_09245
GPNPan110_10840
MRIMal4_32630
SDYNMal52_56780
PLONPla110_12950
GESVT84_21430
GOGC1280_17025
GMSSOIL9_19980
TIMGMBLW1_08230
LRSPX52LOC_05488
FTJFTUN_3009
ULIETAA1_52250
TSPHKIH39_16745
IPAIsop_3055
SACISinac_1245
PBORBSF38_00173
AGVOJF2_59190
AMOAnamo_1139
MOXDAMO_0470
MJAMJ_1427
MFEMefer_1394
MVUMetvu_0740
MFSMFS40622_0626
MIFMetin_0374
MJHJH146_0550
MESGMLAUSG7_0642
MESAMLASG1_0183
MIGMetig_1450
MMPMMP0279(mptG)
MMQMmarC5_1394
MMXMmarC6_0674
MMZMmarC7_1282
MMDGYY_01430
MMAKMMKA1_02860
MMAOMMOS7_03130
MMADMMJJ_07790
MAEMaeo_0244
MVNMevan_1290
MVOMvol_1337
MOKMetok_1124
METFCFE53_00770
MTHMTH_830
MMGMTBMA_c12280
MWOMWSIV6_1207
MTEEMTTB_05440
METCMTCT_0744
METEtca_00797(thrB_2)
METZMETMT2_0904
METKFVF72_07390
MTHMFZP57_05730
METJFZP68_00475
MSTMsp_0373
METBAW729_05205
MRUmru_1690(mptG)
MSIMsm_0848
MEBAbm4_0511(mptG1) Abm4_0513(mptG2)
MMILsm9_1728(mptG1) sm9_1774(mptG2)
MEYETL18_04245
MOLYLM1_1158
MAROMarbSA_04300 MarbSA_18790
METVK4897_07240
MELMetbo_1945
MEWMSWAN_0675
METHMBMB1_0695
MFCBRM9_1500(mptG)
MFIDSM1535_2194
MCUBMCBB_0706
MSUBBK009_01695
METNBK008_09230
METTCIT01_01960
METOCIT02_03295
MEMEHYG87_03485
MFVMfer_0206
MKAMK0558
AFUAF_2089
AFGAFULGI_00023580
APOArcpr_1537
AVEArcve_1291
ASTAsulf_00664
FPLFerp_2137
GACGACE_1235
GAHGAH_01805
PFUPF0903
PFIPFC_03735
PHOPH0227(PH0227) PH1228(PH1228)
PABPAB0141 PAB1694
PYNPNA2_0739 PNA2_1695
PYAPYCH_05770
PYSPy04_0291 Py04_0969
PYCTQ32_00530 TQ32_05075
TONTON_0388
TGATGAM_1652(ghmP)
TSITSIB_0667
TBATERMP_00443
THEGQS_03285
THATAM4_1771 TAM4_77
THMCL1_0331
TLTOCC_00267
THSTES1_0523
TNUBD01_0324 BD01_1268
TEUTEU_07325
TGYX802_01185
THVADU37_CDS13370
TCHCHITON_0355 CHITON_0721
TPEPA0127_00455 A0127_04160
TGGA3K92_04075
TCEA3L02_09400
TBSA3L01_02635
THHCDI07_02590
TSLA3L11_06580
TTDA3L14_04180
TPRFA3L09_02260 A3L09_05405
TRLA3L10_03945
TPAFA3L08_05635
THYA3L12_06105
TICFH039_04965
TCQTIRI35C_0752
THEMFPV09_04220
PPACPAP_03835
MBAMbar_A0806 Mbar_A1222
MBYMSBRM_0510 MSBRM_3298
MBWMSBRW_0196 MSBRW_3502
MBARMSBR2_1867 MSBR2_3253
MBAKMSBR3_2057 MSBR3_3248
MACMA_0339 MA_4006
MMAMM_0905 MM_1592
MMAZMmTuc01_0925 MmTuc01_1660
MMJMSMAS_0400 MSMAS_3077
MMACMSMAC_0419 MSMAC_3096
MVCMSVAZ_3098 MSVAZ_3286
MEKMSKOL_3060 MSKOL_3248
MLSMSLAZ_0207 MSLAZ_0542
METMMSMTP_0427 MSMTP_2976 MSMTP_2977
MEFMSWH1_0247 MSWH1_0384
MEQMSWHS_0257 MSWHS_0393
MSJMSSAC_0326 MSSAC_3740
MSZMSSIH_0284 MSSIH_3276
MSWMSSIT_0290 MSSIT_3347
MTHRMSTHT_1515 MSTHT_2168
MTHEMSTHC_1111 MSTHC_1774
MHORMSHOH_0494 MSHOH_3797
MFZAOB57_006660
MBUMbur_1563
MMETMCMEM_2074
MELOJ7W08_09335
MMHMmah_0135
MHAZBHR79_02750
MEVMetev_2206
MZHMzhil_1969
MPYMpsy_1935
MZIHWN40_12540
MHZMetho_0055
MTPMthe_0935
MCJMCON_0135 MCON_2372
MHIMhar_0292 Mhar_2046
MHUMhun_0932 Mhun_1148
MRTJKHC33_08360 KHC33_09045
MLAMlab_0788
MEMMemar_0701 Memar_1082
MBGBN140_1813 BN140_2219
MEMAMMAB1_2341 MMAB1_2941
MCHKMchiMG62_08240 MchiMG62_12220
MPIMpet_0657 Mpet_2520
MBNMboo_1451
MFOMetfor_0371 Metfor_1710
MPLMpal_1624 Mpal_1826
MPDMCP_1555(mptG-1) MCP_1942(mptG-2)
MEZMtc_1505
RCILRC185 LRC387
HDLHALDL1_13540
HHBHhub_3126
HNOLT974_12090
HLULT972_02800
HJEHacjB3_05855
HSUHLASF_1754
HSFHLASA_1740
SALRFQU85_06750
HALREFA46_001285
HAADMW046_11795
HALXM0R89_02715
HAXZM0R88_02705
SALIL593_05040
SAIMK0C01_00545
HARAAArcS_0282
HMArrnAC0303(ghmP)
HHIHAH_1044(ghmP)
HHNHISP_05365
HABSG26_13035
HTABVU17_05110
HALJG9465_16560
HSINKDQ40_08270
NPHNP_4930A
NMONmlp_3439
NHOHWV23_00245
NSALHWV07_18670
HUTHuta_2356
HTIHTIA_1898
HALAHrd1104_01295
HMUHmuk_1348
HALZE5139_09900
HALLLC1Hm_0569
HAZPGBQ70_09895
HALIBV210_13160
HSNDV733_13570
HDSHSR122_2326
HRRHZS55_20330
HPELHZS54_23815
HLTI7X12_14695
HARCHARCEL1_05620
HDOMUK72_08345
HWAHQ_3191A
HWCHqrw_3752
HVOHVO_2628
HMEHFX_2645
HGIABY42_13195
HALEG3A49_16850
HLSKU306_15520
HBOHbor_09470
HAQDU484_17655
HAJDU500_00695
HAERDU502_16075
HRAEI982_14860
HLMDV707_14730
HALMFCF25_02680
HLAHlac_2038
HALPDOS48_10605
HALBEKH57_15200
HEZZEO776_11760
HALQHrr1229_009915
HSSJ7656_04310
HSAIHPS36_09855
HAZZKI388_10220
SRUBC2R22_16195
HAEhalTADL_2288
HALNB4589_012620
HALGHUG10_10075
HALUHUG12_07025
HMPK6T50_06940
HREK6T36_06730
HRMK6T25_09115
HACBHbl1158_12655
HAHHalar_1989
HTUHtur_2109
HDABB347_05420
HSALJMJ58_18320
HLOJ0X27_05050
HAKZJ0X25_17110
NMGNmag_0626
HXAHalxa_2722
NATNJ7G_1238
NPENatpe_2068
NVRFEJ81_09130
NPLFGF80_03685
NAYHYG81_02260
HALYHYG82_05345
HJTDVR14_18435
NGENatgr_0402
HRUHalru_2014
NOUNatoc_3775
HLRHALLA_14180
HLCCHINAEXTREME12750
NAJB1756_14620
NAGAArcMg_2217
NANAArc1_1469
NBGDV706_15405
NASGCU68_15670
SAWLNGM29_07630
MELUMTLP_10770
MEAMMU439_01185
APEAPE_1512 APE_2425
ACJACAM_0950 ACAM_1521
SHCShell_1377
IHOIgni_1441
IISEYM_04885
DKADKAM_0197
DFDDesfe_0276
DMUDesmu_0983
TAGTagg_0600
IAGIgag_0281 Igag_0656
THGTCELL_1263
HBUHbut_0952
PFMPyrfu_0027 Pyrfu_1305
PDLPyrde_0679 Pyrde_1683
STOSTK_13890
SOHD1869_07830
SSOSSO0370
SOLSsol_1345
SSOASULA_1388
SSOLSULB_1389
SSOFSULC_1387
SSHIJ5U23_03069
SCASSACC_02810
SAISaci_1486
SACNSacN8_07210
SACRSacRon12I_07210
SACSSUSAZ_07060
SISLS215_1873
SIAM1425_1733
SIMM1627_1851
SIDM164_1780
SIYYG5714_1849
SINYN1551_1076
SIILD85_1990
SIHSiH_1709
SIRSiRe_1629
SICSiL_1622
SULABFU36_03980
SULEGFS03_09740
SULOGFS33_01555
SULLEWF20_09000
MSEMsed_1616
MEMJMJ1HA_1841
MCNMcup_0611
MHKDFR87_10500
MPRUDFR88_10195
MTENGWK48_01755
MJNMjAS7_2727
AHOAhos_1268
AMANB6F84_10775
ABRIDFR85_10035
ASULDFR86_09115
AAMBD1866_01250
ACIHHS5_26700
SACDHS1genome_0919
SAZOD1868_02875
CSTYKN1_20760
STEPIC006_1230
SAHSHS7_18400
ASCASAC_0184 ASAC_0528
CLGCalag_0385 Calag_1478
FFOFFONT_0809
CSUCSUB_C1261
LOKILokiarch_00720
PSYTDSAG12_01806
 » show all
Reference
1  [PMID:9698382]
  Authors
Rasche ME, White RH
  Title
Mechanism for the enzymatic formation of 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate during the biosynthesis of methanopterin.
  Journal
Biochemistry 37:11343-51 (1998)
DOI:10.1021/bi973086q
Reference
2  [PMID:12142414]
  Authors
Scott JW, Rasche ME
  Title
Purification, overproduction, and partial characterization of beta-RFAP synthase, a key enzyme in the methanopterin biosynthesis pathway.
  Journal
J Bacteriol 184:4442-8 (2002)
DOI:10.1128/JB.184.16.4442-4448.2002
  Sequence
[afu:AF_2089]
Reference
3  [PMID:15262968]
  Authors
Dumitru RV, Ragsdale SW
  Title
Mechanism of 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate synthase, a key enzyme in the methanopterin biosynthetic pathway.
  Journal
J Biol Chem 279:39389-95 (2004)
DOI:10.1074/jbc.M406442200
  Sequence
[mja:MJ_1427]
Reference
4  [PMID:21634403]
  Authors
White RH
  Title
The conversion of a phenol to an aniline occurs in the biochemical formation of the 1-(4-aminophenyl)-1-deoxy-D-ribitol moiety in methanopterin.
  Journal
Biochemistry 50:6041-52 (2011)
DOI:10.1021/bi200362w
  Sequence
[mja:MJ_1427]
Reference
5  [PMID:31663056]
  Authors
Bechard ME, Farahani P, Greene D, Pham A, Orry A, Rasche ME.
  Title
Purification, kinetic characterization, and site-directed mutagenesis of Methanothermobacter thermautotrophicus RFAP Synthase Produced in Escherichia coli.
  Journal
AIMS Microbiol 5:186-204 (2019)
DOI:10.3934/microbiol.2019.3.186
Other DBs
ExplorEnz - The Enzyme Database: 2.4.2.54
IUBMB Enzyme Nomenclature: 2.4.2.54
ExPASy - ENZYME nomenclature database: 2.4.2.54
BRENDA, the Enzyme Database: 2.4.2.54
LinkDB

DBGET integrated database retrieval system