KEGG ENZYME: 2.4.2.54

ENZYME: 2.4.2.54

Entry

EC 2.4.2.54                 Enzyme

Name

beta-ribofuranosylphenol 5'-phosphate synthase;
beta-RFAP synthase (incorrect);
beta-RFA-P synthase (incorrect);
AF2089 (gene name);
MJ1427 (gene name);
beta-ribofuranosylhydroxybenzene 5'-phosphate synthase;
4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate synthase (incorrect);
beta-ribofuranosylaminobenzene 5'-phosphate synthase (incorrect);
5-phospho-alpha-D-ribose 1-diphosphate:4-aminobenzoate 5-phospho-beta-D-ribofuranosyltransferase (decarboxylating) (incorrect)

Class

Transferases;
Glycosyltransferases;
Pentosyltransferases

Sysname

5-phospho-alpha-D-ribose-1-diphosphate:4-hydroxybenzoate 5-phospho-beta-D-ribofuranosyltransferase (decarboxylating)

Reaction(IUBMB)

5-phospho-alpha-D-ribose 1-diphosphate + 4-hydroxybenzoate = 4-(beta-D-ribofuranosyl)phenol 5'-phosphate + CO2 + diphosphate [RN:R11102]

Reaction(KEGG)

R11102;
(other) R10337

Substrate

5-phospho-alpha-D-ribose 1-diphosphate [CPD:C00119];
4-hydroxybenzoate [CPD:C00156]

Product

4-(beta-D-ribofuranosyl)phenol 5'-phosphate [CPD:C21107];
CO2 [CPD:C00011];
diphosphate [CPD:C00013]

Comment

The enzyme is involved in biosynthesis of tetrahydromethanopterin in archaea. It can utilize both 4-hydroxybenzoate and 4-aminobenzoate as substrates, but only the former is known to be produced by methanogenic archaea [4]. The activity is dependent on Mg2+ or Mn2+ [1].

History

EC 2.4.2.54 created 2013, modified 2014, modified 2015

Pathway

ec00790

Folate biosynthesis

Orthology

K06984

beta-ribofuranosylaminobenzene 5'-phosphate synthase

Genes

PTAI:

ICN73_03205

SFR:

Sfri_3142

SBJ:	CF168_02950

PSEO:

OM33_01310

MCA:

MCA2861

METU:

GNH96_03540

MMEO:

OOT43_14025

MEEF:

JWZ97_16205

MMT:	Metme_1363 Metme_2289

MDN:	JT25_019570

MDH:	AYM39_07270 AYM39_11925

MKO:	MKLM6_2459

METL:

U737_09895

MPAD:

KEF85_11140

MELL:

IVG45_02775

MRP:	NM686_009760

MMOT:

QZJ86_10325

MEEP:

JWZ98_13645

MEEC:

PL263_03065

MEUP:

QC632_14025

MAH:	MEALZ_2423(mptG)

MBUR:

EQU24_09135

MPSY:

CEK71_02825

MMAI:

sS8_3396

MSZE:

MSZNOR_2919

MMOB:

F6R98_02835

MEIN:

methR_P2330

MOZ:	MoryE10_28910

MISZ:

MishRS11D_40040

MESL:

KKZ03_09065

MECH:

Q9L42_015125

MEIY:

MIN45_P1929

MCAU:

MIT9_P1439

MEJ:

Q7A_615

MEC:

Q7C_2201

MMAF:

GCM100_12070

MTHA:

VSX76_03935

BLEP:

AL038_03750

NHL:

Nhal_3873

NWR:	E3U44_04690

TSY:	THSYN_31795

SEDS:

AAY24_01635

NIK:	F5I99_01510

COX:	E0W60_31915

BYI:	BYI23_B014490

BUE:	BRPE67_BCDS01480

BPH:	Bphy_5949 Bphy_6481

BCAI:

K788_0001553 K788_0008555

PARA:

BTO02_24140

PHS:	C2L64_21830 C2L64_38310

PTER:

C2L65_26140 C2L65_34305

PSAA:

QEN71_34100

BUO:	BRPE64_BCDS01620

AFA:	UZ73_08470

VAP:	Vapar_3085

VPD:	VAPA_1c31490

MPT:

Mpe_A2606

METP:

C1M51_15110

RGE:	RGE_40220(mptG)

LCH:

Lcho_3153

AON:	DEH84_19010

METR:

BSY238_3295

MFA:

Mfla_1661

MMB:

Mmol_1347

MEH:

M301_1557

MEI:	Msip34_1505

MEP:

MPQ_1568

MBAC:

BN1209_0744

MEU:	ACJ67_08185

MPAU:

ZMTM_11810

SPLB:

SFPGR_26010

FLE:	KI610_12870

AZA:	AZKH_p0276(mptG)

AZD:	CDA09_11300

THU:	AC731_004835

THAU:

C4PIVTH_3696

THK:	CCZ27_13260

DDZ:	DSYM_25660

MCI:	Mesci_4257

MCIC:

A4R28_05770

MESW:

A9K65_023605

MESM:

EJ066_29705

MOH:	IHQ72_13890

MMEI:

LRP31_14020

MES:

Meso_2350

PHT:	BLM14_23775

RGA:	RGR602_PC01565

NPM:	QEO92_19205

NHA:

Nham_0792

XAU:

Xaut_3535

XDI:	EZH22_18280

STAR:

G3545_09765

LNE:	FZC33_09320

SNO:

Snov_0753

ANC:	GBB76_00765

APRA:

G3A50_16510

APOL:

K9D25_20175

MEA:	Mex_1p1760(mptG)

MDI:	METDI2512(mptG)

MEX:

Mext_1828

MCH:

Mchl_2163

MPO:

Mpop_1779

MZA:	B2G69_16800

MRD:	Mrad2831_0527

MET:

M446_5777

MNO:

Mnod_6001

MOR:

MOC_0682

META:

Y590_08755

MAQU:

Maq22A_c16455(mptG)

MPHY:

MCBMB27_00104

MEE:	DA075_05690

METD:

C0214_10510

METX:

A3862_28315

METS:

DK389_20640

METI:

DK427_18430

MMES:

MMSR116_16675

MTEA:

DK419_10780

MIND:

mvi_45880

MOG:	MMB17_20040

MTAD:

M6G65_13680

MSL:

Msil_2384

MTUN:

MTUNDRAET4_2720

MLG:	CWB41_06030

MEDK:

QEV83_11785

HDN:

Hden_1480

HDT:	HYPDE_31243

HMC:	HYPMC_1630(mptG)

HNI:	W911_13160

FIL:	BN1229_v1_0589(mptG)

FIY:	BN1229_v1_0591(mptG)

MCG:

GL4_1789

METG:

HT051_10165

MSC:

BN69_2822

MBRY:

B1812_13625

MROS:

EHO51_01955

MHEY:

H2LOC_017505

MPAR:

F7D14_03130

MIWA:

SS37A_06870

MESZ:

LNB28_05730

MTW:	CQW49_10280

MECQ:

MSC49_34710

CMET:

K6K41_25490

PLEO:

OHA_1_01038

HDI:

HDIA_2309

AALA:

IGS74_05795

AALM:

LUX29_06550

AUZ:	Sa4125_38500

AUB:	LXB15_00995

JIE:	OH818_26045

JAV:	OXU80_18185

GBE:	GbCGDNIH1_0776

GBH:	GbCGDNIH2_0776

GBC:	GbCGDNIH3_0776

GBS:	GbCGDNIH4_0776

ALI:	AZOLI_p10689 AZOLI_p10892

ABQ:	ABAZ39_25455

ABF:	AMK58_22345

ATI:	AL072_27065

AZT:	TSH58p_01190

AZM:	DM194_17090

AOZ:	HUE56_08935

AARE:

D3093_24975

AZS:	E6C72_18260 E6C72_22445

SULR:

B649_07545

DOV:	DSCO28_33840

DMM:	dnm_005860

SLP:

Slip_0785

PTH:

PTH_0622

DAE:

Dtox_2738

FWA:	DCMF_03860

DGI:	Desgi_4130

HAS:	Halsa_0938

NTP:	CRH09_08300

SLIA:

HA039_21050

BROO:

brsh051_10040

ACIR:

OG979_08480

SAQ:

Sare_0579

RBA:

RB3940

PSL:

Psta_2630

PIR:	VN12_20990

RUL:

UC8_36510

MFF:	MFFC18_33300

ROL:	CA51_37630

RUV:

EC9_40690

RCF:	Poly24_42900

AHEL:

Q31a_33700

LCRE:

Pla8534_63820

AAGG:

ETAA8_20720

BVO:	Pan97_26050

BREM:

PSR63_26485

RLC:	K227x_12670

SMAM:

Mal15_09400

SNEP:

Enr13x_63690

LPAV:

PLANPX_2715

LLH:

I41_29340

AMUC:

Pan181_13200

PND:	Pla175_32920

AMOB:

HG15A2_24450

BGOK:

Pr1d_28070

PLM:

Plim_2423

PEH:	Spb1_32070

PBS:	Plabr_3503

PLS:	VT03_17505

PLH:	VT85_12910

FMR:	Fuma_00726

GMR:	GmarT_11040

GIM:	F1728_09245

GPN:	Pan110_10840

GFM:	Enr17x_11850

GAZ:	Pan241w_12000

GAW:	V144x_46780

GAX:	Pan161_24060

MRI:	Mal4_32630

SDYN:

Mal52_56780

PLON:

Pla110_12950

SVP:	Pan189_25090

TPOL:

Mal48_46960

CCOS:

Pan44_08850

CCOP:

Mal65_44460

GES:	VT84_21430

GOG:	C1280_17025

GMS:	SOIL9_19980

TIM:	GMBLW1_08230

LRS:	PX52LOC_05488

FTJ:

FTUN_3009

ULI:	ETAA1_52250

TSPH:

KIH39_16745

IPA:

Isop_3055

SACI:

Sinac_1245

PBOR:

BSF38_00173

AGV:	OJF2_59190

AMO:	Anamo_1139

MOX:

DAMO_0470

MJA:

MJ_1427

MFE:	Mefer_1394

MVU:	Metvu_0740

MFS:	MFS40622_0626

MIF:	Metin_0374

MJH:	JH146_0550

MESG:

MLAUSG7_0642

MESA:

MLASG1_0183

MIG:	Metig_1450

MMP:	MMP0279(mptG)

MMQ:	MmarC5_1394

MMX:	MmarC6_0674

MMZ:	MmarC7_1282

MMD:

GYY_01430

MMAK:

MMKA1_02860

MMAO:

MMOS7_03130

MMAD:

MMJJ_07790

MAE:

Maeo_0244

MVN:	Mevan_1290

MVO:

Mvol_1337

MOK:	Metok_1124

METF:

CFE53_00770

MTH:

MTH_830

MMG:	MTBMA_c12280

MWO:	MWSIV6_1207

MTEE:

MTTB_05440

METC:

MTCT_0744

METE:

tca_00797(thrB_2)

METZ:

METMT2_0904

METK:

FVF72_07390

MTHM:

FZP57_05730

METJ:

FZP68_00475

MST:

Msp_0373

METB:

AW729_05205

MEIS:

PXD04_05675

MRU:	mru_1690(mptG)

MSI:

Msm_0848

MEB:	Abm4_0511(mptG1) Abm4_0513(mptG2)

MMIL:

sm9_1728(mptG1) sm9_1774(mptG2)

MEYE:

TL18_04245

MOL:

YLM1_1158

MARO:

MarbSA_04300 MarbSA_18790

METV:

K4897_07240

MEL:	Metbo_1945

MEW:	MSWAN_0675

METH:

MBMB1_0695

MFC:	BRM9_1500(mptG)

MFI:	DSM1535_2194

MCUB:

MCBB_0706

MSUB:

BK009_01695

METN:

BK008_09230

METT:

CIT01_01960

METO:

CIT02_03295

MEME:

HYG87_03485

MFEG:

GCM10025860_17650

MFV:

Mfer_0206

MKA:

MK0558

AFU:

AF_2089

AFG:	AFULGI_00023580

APO:	Arcpr_1537

AVE:	Arcve_1291

AST:	Asulf_00664

FPL:

Ferp_2137

GAC:

GACE_1235

GAH:

GAH_01805

PFU:

PF0903

PFI:

PFC_03735

PHO:	PH0227(PH0227) PH1228(PH1228)

PAB:	PAB0141 PAB1694

PYN:	PNA2_0739 PNA2_1695

PYA:	PYCH_05770

PYS:	Py04_0291 Py04_0969

PYC:	TQ32_00530 TQ32_05075

TON:

TON_0388

TGA:	TGAM_1652(ghmP)

TSI:

TSIB_0667

TBA:	TERMP_00443

THE:

GQS_03285

THA:	TAM4_1771 TAM4_77

THM:

CL1_0331

TLT:

OCC_00267

THS:

TES1_0523

TNU:	BD01_0324 BD01_1268

TEU:

TEU_07325

TGY:	X802_01185

THV:	ADU37_CDS13370

TCH:	CHITON_0355 CHITON_0721

TPEP:

A0127_00455 A0127_04160

TGG:	A3K92_04075

TCE:	A3L02_09400

TBS:	A3L01_02635

THH:	CDI07_02590

TSL:	A3L11_06580

TTD:	A3L14_04180

TPRF:

A3L09_02260 A3L09_05405

TRL:	A3L10_03945

TPAF:

A3L08_05635

THY:	A3L12_06105

TIC:	FH039_04965

TCQ:	TIRI35C_0752

THEM:

FPV09_04220

PPAC:

PAP_03835

MBA:	Mbar_A0806 Mbar_A1222

MBY:	MSBRM_0510 MSBRM_3298

MBW:	MSBRW_0196 MSBRW_3502

MBAR:

MSBR2_1867 MSBR2_3253

MBAK:

MSBR3_2057 MSBR3_3248

MAC:	MA_0339 MA_4006

MMA:	MM_0905 MM_1592

MMAZ:

MmTuc01_0925 MmTuc01_1660

MMJ:	MSMAS_0400 MSMAS_3077

MMAC:

MSMAC_0419 MSMAC_3096

MVC:	MSVAZ_3098 MSVAZ_3286

MEK:	MSKOL_3060 MSKOL_3248

MLS:	MSLAZ_0207 MSLAZ_0542

METM:

MSMTP_0427 MSMTP_2976 MSMTP_2977

MEF:	MSWH1_0247 MSWH1_0384

MEQ:	MSWHS_0257 MSWHS_0393

MSJ:	MSSAC_0326 MSSAC_3740

MSZ:	MSSIH_0284 MSSIH_3276

MSW:	MSSIT_0290 MSSIT_3347

MTHR:

MSTHT_1515 MSTHT_2168

MTHE:

MSTHC_1111 MSTHC_1774

MHOR:

MSHOH_0494 MSHOH_3797

MFZ:	AOB57_006660

MBU:

Mbur_1563

MMET:

MCMEM_2074

MELO:

J7W08_09335

MMH:

Mmah_0135

MHAZ:

BHR79_02750

MPOT:

BKM01_05550

MEV:	Metev_2206

MZH:	Mzhil_1969

MPY:

Mpsy_1935

MZI:	HWN40_12540

MMAV:

RE476_10985

MSEB:

RE474_07560

MHZ:	Metho_0055

MEHF:

MmiHf6_11610

MEES:

MmiEs2_14270

MTP:

Mthe_0935

MCJ:	MCON_0135 MCON_2372

MHI:	Mhar_0292 Mhar_2046

MHU:	Mhun_0932 Mhun_1148

MRTJ:

KHC33_08360 KHC33_09045

MLA:

Mlab_0788

MEM:	Memar_0701 Memar_1082

MBG:	BN140_1813 BN140_2219

MEMA:

MMAB1_2341 MMAB1_2941

MCHK:

MchiMG62_08240 MchiMG62_12220

MSUM:

OH143_10225 OH143_12135

MRC:	R6Y96_06425 R6Y96_09340

MPI:	Mpet_0657 Mpet_2520

MEFW:

F1737_07755

MAQE:

RJ40_12620

MFK:	E2N92_00255

MOU:	OU421_11395

MESB:

L1S32_03285

MANQ:

L1994_08255

MBN:

Mboo_1451

MFO:	Metfor_0371 Metfor_1710

MPL:	Mpal_1624 Mpal_1826

MPD:	MCP_1555(mptG-1) MCP_1942(mptG-2)

MEZ:

Mtc_1505

RCI:	LRC185 LRC387

MJK:	N2V74_00730 N2V74_05130

HDL:	HALDL1_13540

HHB:

Hhub_3126

HNO:	LT974_12090

HLU:	LT972_02800

HSU:	HLASF_1754

HSF:	HLASA_1740

HAHS:

HSRCO_2341

SALR:

FQU85_06750

HALR:

EFA46_001285

HJE:	HacjB3_05855

HDO:	MUK72_08345

HALX:

M0R89_02715

HAXZ:

M0R88_02705

HAYC:

NGM10_12460

HAXY:

NGM07_06925

SALI:

L593_05040

SAIM:

K0C01_00545

HARA:

AArcS_0282

HMA:	rrnAC0303(ghmP)

HHI:	HAH_1044(ghmP)

HHN:	HISP_05365

HAB:	SG26_13035

HTA:	BVU17_05110

HALJ:

G9465_16560

HSIN:

KDQ40_08270

NPH:

NP_4930A

NMO:

Nmlp_3439

NHO:	HWV23_00245

NSAL:

HWV07_18670

HUT:

Huta_2356

HTI:

HTIA_1898

HALA:

Hrd1104_01295

HASV:

SVXHr_2205

HABN:

HBNXHr_2198

HMU:

Hmuk_1348

HALZ:

E5139_09900

HALL:

LC1Hm_0569

HAZP:

GBQ70_09895

HALI:

BV210_13160

HSN:	DV733_13570

HDS:	HSR122_2326

HRR:	HZS55_20330

HPEL:

HZS54_23815

HLT:	I7X12_14695

HARC:

HARCEL1_05620

SSAI:

N0B31_10135

HAAD:

MW046_11795

HWA:

HQ_3191A

HWC:

Hqrw_3752

HVO:

HVO_2628

HALE:

G3A49_16850

HLN:	SVXHx_1969

HME:

HFX_2645

HGI:	ABY42_13195

HLS:	KU306_15520

HBO:	Hbor_09470

HAQ:	DU484_17655

HAJ:	DU500_00695

HAER:

DU502_16075

HRA:	EI982_14860

HLM:	DV707_14730

HALM:

FCF25_02680

HLA:

Hlac_2038

HALP:

DOS48_10605

HALB:

EKH57_15200

HEZZ:

EO776_11760

HALQ:

Hrr1229_009915

HSS:	J7656_04310

HSAI:

HPS36_09855

HAZZ:

KI388_10220

SRUB:

C2R22_16195

HAE:	halTADL_2288

HALN:

B4589_012620

HALG:

HUG10_10075

HALU:

HUG12_07025

HMP:	K6T50_06940

HRE:	K6T36_06730

HRM:	K6T25_09115

HACB:

Hbl1158_12655

HAH:	Halar_1989

HTU:

Htur_2109

HSAL:

JMJ58_18320

HAKZ:

J0X25_17110

NMG:

Nmag_0626

HXA:	Halxa_2722

NAT:

NJ7G_1238

NPE:	Natpe_2068

NVR:	FEJ81_09130

NPL:	FGF80_03685

NAY:	HYG81_02260

HALY:

HYG82_05345

HJT:	DVR14_18435

HLO:	J0X27_05050

NGE:	Natgr_0402

HRU:	Halru_2014

SAWL:

NGM29_07630

HALV:

NGM15_00140

NOU:	Natoc_3775

HLR:	HALLA_14180

HLC:	CHINAEXTREME12750

NAJ:	B1756_14620

NAG:	AArcMg_2217

NAN:	AArc1_1469

NARA:

QQ977_02755

NBG:	DV706_15405

NAS:	GCU68_15670

HDA:	BB347_05420

MELU:

MTLP_10770

MEAM:

MU439_01185

MEAE:

QEN48_01395

APE:	APE_1512 APE_2425

ACJ:	ACAM_0950 ACAM_1521

SHC:	Shell_1377

IHO:

Igni_1441

IIS:

EYM_04885

DKA:

DKAM_0197

DFD:	Desfe_0276

DMU:	Desmu_0983

TAG:

Tagg_0600

IAG:	Igag_0281 Igag_0656

THG:	TCELL_1263

HBU:

Hbut_0952

PFM:	Pyrfu_0027 Pyrfu_1305

PDL:	Pyrde_0679 Pyrde_1683

PABI:

PABY_04380

PARE:

PYJP_20570

STO:

STK_13890

SOH:	D1869_07830

SSO:

SSO0370

SOL:

Ssol_1345

SSOA:

SULA_1388

SSOL:

SULB_1389

SSOF:

SULC_1387

SSHI:

J5U23_03069

SCAS:

SACC_02810

SAI:

Saci_1486

SACN:

SacN8_07210

SACR:

SacRon12I_07210

SACS:

SUSAZ_07060

SIS:	LS215_1873

SIA:	M1425_1733

SIM:	M1627_1851

SID:

M164_1780

SIY:	YG5714_1849

SIN:	YN1551_1076

SII:

LD85_1990

SIH:

SiH_1709

SIR:

SiRe_1629

SIC:

SiL_1622

SULA:

BFU36_03980

SULE:

GFS03_09740

SULO:

GFS33_01555

SULL:

EWF20_09000

MSE:

Msed_1616

MEMJ:

MJ1HA_1841

MCN:

Mcup_0611

MHK:	DFR87_10500

MPRU:

DFR88_10195

MTEN:

GWK48_01755

MJN:	MjAS7_2727

AHO:

Ahos_1268

AMAN:

B6F84_10775

ABRI:

DFR85_10035

ASUL:

DFR86_09115

AAMB:

D1866_01250

ACIH:

HS5_26700

SACD:

HS1genome_0919

SAZO:

D1868_02875

CSTY:

KN1_20760

STEP:

IC006_1230

SMET:

RQ359_000680

SAHS:

HS7_18400

ASC:	ASAC_0184 ASAC_0528

CLG:	Calag_0385 Calag_1478

FFO:	FFONT_0809

CSU:	CSUB_C1261

LOKI:

Lokiarch_00720

LOB:	NEF87_004229

PSYT:

DSAG12_01806

» show all

Reference

1 [PMID:9698382]

Authors

Rasche ME, White RH

Title

Mechanism for the enzymatic formation of 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate during the biosynthesis of methanopterin.

Journal

Biochemistry 37:11343-51 (1998)
DOI:10.1021/bi973086q

Reference

2 [PMID:12142414]

Authors

Scott JW, Rasche ME

Title

Purification, overproduction, and partial characterization of beta-RFAP synthase, a key enzyme in the methanopterin biosynthesis pathway.

Journal

J Bacteriol 184:4442-8 (2002)
DOI:10.1128/JB.184.16.4442-4448.2002

Sequence

[afu:AF_2089]

Reference

3 [PMID:15262968]

Authors

Dumitru RV, Ragsdale SW

Title

Mechanism of 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate synthase, a key enzyme in the methanopterin biosynthetic pathway.

Journal

J Biol Chem 279:39389-95 (2004)
DOI:10.1074/jbc.M406442200

Sequence

[mja:MJ_1427]

Reference

4 [PMID:21634403]

Authors

White RH

Title

The conversion of a phenol to an aniline occurs in the biochemical formation of the 1-(4-aminophenyl)-1-deoxy-D-ribitol moiety in methanopterin.

Journal

Biochemistry 50:6041-52 (2011)
DOI:10.1021/bi200362w

Sequence

[mja:MJ_1427]

Reference

5 [PMID:31663056]

Authors

Bechard ME, Farahani P, Greene D, Pham A, Orry A, Rasche ME.

Title

Purification, kinetic characterization, and site-directed mutagenesis of Methanothermobacter thermautotrophicus RFAP Synthase Produced in Escherichia coli.

Journal

AIMS Microbiol 5:186-204 (2019)
DOI:10.3934/microbiol.2019.3.186

Other DBs

ExplorEnz - The Enzyme Database:

2.4.2.54

IUBMB Enzyme Nomenclature:

2.4.2.54

ExPASy - ENZYME nomenclature database:

2.4.2.54

BRENDA, the Enzyme Database:

2.4.2.54

LinkDB

DBGET integrated database retrieval system