KEGG ENZYME: 2.5.1.101

ENZYME: 2.5.1.101

Entry

EC 2.5.1.101                Enzyme

Name

N,N'-diacetyllegionaminate synthase;
neuB (gene name);
legI (gene name)

Class

Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)

Sysname

phosphoenolpyruvate:2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose 1-(2-carboxy-2-oxoethyl)transferase

Reaction(IUBMB)

2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O = N,N'-diacetyllegionaminate + phosphate [RN:R10304]

Reaction(KEGG)

R10304

Substrate

2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose [CPD:C20424];
phosphoenolpyruvate [CPD:C00074];
H2O [CPD:C00001]

Product

N,N'-diacetyllegionaminate [CPD:C20418];
phosphate [CPD:C00009]

Comment

Requires a divalent metal such as Mn2+. Isolated from the bacteria Legionella pneumophila and Campylobacter jejuni, where it is involved in the biosynthesis of legionaminic acid, a virulence-associated, cell surface sialic acid-like derivative.

History

EC 2.5.1.101 created 2012

Pathway

ec00541

Biosynthesis of various nucleotide sugars

ec01100

Metabolic pathways

Orthology

K18430

N,N'-diacetyllegionaminate synthase

Genes

PMAI:

CF386_03205(neuB)

PSOA:

PSm6_37510(neuB)

MLQ:	ASQ50_04385

MARJ:

MARI_04460(legI)

MSAN:

LPB19_15080(neuB)

LPN:	lpg0752(neuB)

LPH:	LPV_0878(neuB)

LPO:	LPO_0833(neuB)

LPU:	LPE509_02459

LPM:	LP6_0732(neuB)

LPF:	lpl0789(neuB)

LPP:	lpp0818(neuB)

LPC:	LPC_2540(neuB)

LPA:

lpa_01160

LPE:

lp12_0762

LCD:	clem_09455(neuB_2)

LWA:	SAMEA4504053_1066(spsE)

LANT:

TUM19329_12800(neuB)

LCAD:

PXX05_08225(neuB)

FPI:	BF30_1411(neuB)

FPX:	KU46_147(neuB)

FHA:	CDV26_09130(neuB)

FRC:	KX01_1747(neuB)

TAO:	THIAE_07350

TANI:

J8380_09295(neuB)

OME:	OLMES_5580

SUTR:

L0B52_04055(neuB)

THIN:

CRN91_03145(neuB)

THO:	SP60_02330

CARI:

FNU76_04765(neuB)

ACHH:

ABFG95_13970(neuB)

RHOB:

HTY51_14425(neuB)

RLH:	MIZ03_4207

POS:	DT070_03545(neuB)

PAQA:

K9V56_020865(neuB)

JAJ:	EKL02_02185(neuB)

METR:

BSY238_2009(neuB)

MRK:	FIT61_02680(neuB)

ARES:

IWH25_01425(neuB)

DECH:

GBK02_12550(neuB)

ACOM:

CEW83_19880(neuB)

RHT:	NT26_3141(neuB)

BRAY:

ML401_23090(neuB)

MCH:

Mchl_3980

DEI:	C4375_03855(neuB)

DEA:	FPZ08_13230(neuB)

DOY:	JI749_13415(neuB)

DRH:	JI748_11510(neuB)

DPV:	ACE4RL_17305(neuB)

BSB:	Bresu_0507

BREU:

FKQ52_02940(neuB)

BSTA:

ACETK8_01295(neuB)

BREN:

KKHFBJBL_03057(neuB)

FAQ:	G5B39_16690(neuB)

GABY:

AB6B38_07365(neuB)

SLAN:

GV829_10185(neuB)

SPMS:

ACO227_09565(neuB)

ALI:	AZOLI_p60097

AZZ:	DEW08_30050(neuB)

MAGX:

XM1_0956

MAG:

amb0080

MAGQ:

MGMAQ_0728

NACI:

NUH88_13130(neuB)

MGM:

Mmc1_0562

HCP:

HCN_1148

HCB:	HCBAA847_0824

HMY:	V3I05_10170(neuB)

TDN:	Suden_0595

SPAL:

FM071_00635(neuB)

SSEI:

FJR45_00975(neuB) FJR45_04420(neuB)

SMAS:

HUE87_09365(neuB)

SMAX:

FJR03_03345(neuB)

SAQT:

GJV85_00945(neuB)

SULR:

B649_08560

HSJ:	WCY03_03780(neuB)

CJE:	Cj1327(neuB2)

CJB:	BN148_1327(neuB2)

CJI:	CJSA_1263(neuB2)

CJS:

CJS3_1421

CJP:	A911_06450

CJEJ:

N564_02255

CJEU:

N565_02345

CJEN:

N755_02405

CJEI:

N135_01415

CJER:

H730_07590

CJY:	QZ67_01463(legI)

CJQ:	UC78_1270(legI)

CJL:	PJ17_07020

CJW:	PJ18_06820

CJR:	CJE1516(ptmC)

CJD:	JJD26997_0385(ptmC)

CJZ:	M635_02330

CJX:	BN867_13120

CCO:	CCC13826_2303(legI)

CCOC:

CCON33237_1630(legI)

CLA:	CLA_1235(legI)

CLL:	CONCH_0304

CCOL:

BN865_08100c

CCC:	G157_02185

CCQ:	N149_1290(legI)

CCF:

YSQ_02215

CCY:

YSS_07200

CCOI:

YSU_02250

CCOF:

VC76_06660(legI)

CCOO:

ATE51_00910(legI)

CAJ:	CIG1485E_0029(legI)

CIS:	CINS_1194(legI)

CVO:	CVOL_1214(legI)

CSM:	CSUB8521_1417(legI)

CSF:	CSUB8523_1517(legI)

CHV:	CHELV3228_1655(legI)

CLX:	CLAN_1241(legI)

CAVI:

CAV_1241(legI)

CAMZ:

CVIC12175_0322(legI)

CAMY:

CSUIS_1328(legI)

COJ:	CORN_1305(legI)

CUX:	CUP3940_0184(legI)

CARM:

CARM_1264(legI)

CMUC:

CMCT_0197(legI)

CNV:	CNZW441b_0374(legI)

CVU:	CVULP_0183(legI)

CDEV:

CIGN_1365(legI)

ABT:

ABED_0620

ATP:

ATR_1813

ACIB:

ACBT_0334

ACRE:

ACRYA_0150

ALAN:

ALANTH_0840

AELL:

AELL_2683

ACLO:

ACLO_2510

ARC:

ABLL_0809

AROS:

NJU99_04380(neuB)

APAI:

APAC_2452

HBV:

ABIV_2490

HCJ:	HCR_18720(legI)

GSB:	GSUB_14715

PACE:

A6070_09825

PEF:	A7E78_05530

SOLI:

ACP59X_09755

DESB:

BuS5_02382

RML:	FF011L_46220(neuB_1)

PLS:	VT03_18850(neuB)

GMR:	GmarT_17240(legI)

GIM:	F1728_06160(neuB)

GPN:	Pan110_17070(legI)

GFM:	Enr17x_19910(legI)

GAZ:	Pan241w_18990(legI)

GAW:	V144x_39890(legI)

GAX:	Pan161_17520(legI)

SING:

V5E97_15220

KNV:	Pan216_23960(neuB)

UAM:	UABAM_06153

SCD:	Spica_0510

LIL:	LA_1609(spsE)

LIE:	LIF_A1296(spsE)

LIC:	LIC_12173(neuB)

LST:

LSS_16461

LAJ:	A0128_07375

LMAY:

DPV73_15970(neuB)

LIOW:

AB3N60_10160(neuB)

GMA:	AciX8_1508

GEX:	GETHOR_25590(spsE)

MSEA:

METESE_01910

BNO:	K8P02_14905(neuB)

TFO:	BFO_1066(neuB)

MGOD:

E7746_11035(neuB)

DUN:	FDZ78_02060(neuB)

DDB:	E7747_10475(neuB)

DUQ:	E7745_12815(neuB)

PII:	NF347_01300(neuB)

COPR:

Cop2CBH44_22070(legI)

ANF:

AQPE_0356

CHIH:

GWR21_01080(neuB) GWR21_20995(neuB)

CSAC:

SIO70_18305(neuB)

PEP:	AQ505_02055

PSTY:

BFS30_06850

PEJ:	FYC62_11660(neuB)

SHG:	Sph21_1815

SPRZ:

VXM68_05900(neuB)

SCN:	Solca_4307

SLAS:

L2B55_03430(neuB)

MMAB:

HQ865_06375(neuB)

AGD:	FRZ59_07155(neuB)

OLI:	FKG96_05940(neuB)

EMAI:

KZP23_21780(neuB)

ALM:	AO498_13850

DEND:

ACN9ML_19770

PACT:

CA264_12450

ASWU:

HUW51_14155(neuB)

MHAA:

Q3Y49_14225(neuB)

MARP:

QYS47_27760(neuB)

MSAA:

QYS49_36485(neuB)

FPF:	DCC35_14845(neuB)

FBT:	D770_00730

FUV:	JR347_14630(neuB)

RCG:	N7E81_05775(neuB)

FEN:	J0383_14250(neuB)

COL:	AM608_07315

MART:

BTR34_08470

MARB:

CJ263_08140(neuB)

NDO:

DDD_3554

NMF:

NMS_2733

POM:	MED152_05685

WIJ:	BWZ20_06350

TJE:	TJEJU_1144(legI)

MUT:	GVT53_17455(neuB)

SAHN:

JRG66_01115(neuB)

CJG:	NCTC13459_02715(spsE)

CANT:

NCTC13489_01541(spsE)

PPH:

Ppha_0527

PMX:	PERMA_1627(neuB)

TTK:	TST_1473(neuB)

DDF:	DEFDS_0370

NLI:	G3M70_11130(neuB)

NWT:	NSPWAT_0140(neuB)

BBAE:

FRD01_15935(neuB)

MEBZ:

LPC09_22465(neuB)

AUI:	APT62_03100

CARC:

NY10_960

CNO:	NT01CX_1784

CBL:

CLK_2101

CBN:

CbC4_0996

CKL:

CKL_2472

CKR:

CKR_2186

CCB:	Clocel_2387

CEU:	A7L45_12145

CFM:	BJL90_06975

CCHV:

BTM20_03650

CGAS:

J1C67_16000(neuB)

CMAH:

C1I91_08395(neuB)

CTAG:

LL095_12335(neuB)

CALE:

FDN13_00880(neuB)

CRS:	FQB35_02420(neuB)

CPRF:

K7H06_14850(neuB)

OCW:	OW730_17315(neuB)

CTH:

Cthe_2641

CTX:	Clo1313_0230

CCL:	Clocl_4180

SVJ:	NQ490_02935(neuB)

OKO:	Ruko_10760(neuB)

DDL:	Desdi_3209

DMT:	DESME_12780

DOR:	Desor_0348

FWA:	DCMF_13725

BPB:	bpr_I0428(neuB1)

RFC:	ACIRUI_13655(neuB)

CLE:	Clole_3572

CBAR:

PATL70BA_2643(legI)

TEB:	T8CH_0143(legI)

CST:	CLOST_0367

TIT:

Thit_0481

TXY:	Thexy_1876

TSH:

Tsac_2668

MTA:

Moth_0756

MTHO:

MOTHE_c07000(legI)

MTHZ:

MOTHA_c07940(legI)

MHUI:

MHLNE_07440(legI)

HOR:	Hore_16950

HPK:	Hprae_0327

HALS:

D7D81_17935(neuB)

IFN:	GM661_06780(neuB) GM661_18120(neuB)

AFT:	BBF96_11100

HPRF:

HLPR_26790(neuB)

SEDM:

RBQ61_06885(neuB)

LPIL:

LIP_2376

FCZ:	IMF26_04555(neuB)

CCHA:

ELD05_07925(neuB)

STID:

G7Z12_16405

PLAK:

A1s21155_00145

SYW:	SYNW0448(neuB)

MPRO:

BJP34_35240

FGI:	OP10G_3269

MAEO:

JRV97_05655(neuB)

FVA:	FV113G1_25420

FUL:	C4N20_01280(neuB)

MMQ:	MmarC5_0506

MMAK:

MMKA1_03760

MAE:

Maeo_0420

MEW:	MSWAN_1357

MSJ:	MSSAC_2015

MSW:	MSSIT_2302

MBU:

Mbur_1586

MEND:

L6E24_13055(neuB)

MESB:

L1S32_02480(neuB)

MFO:	Metfor_1307

» show all

Reference

1 [PMID:18275154]

Authors

Glaze PA, Watson DC, Young NM, Tanner ME

Title

Biosynthesis of CMP-N,N'-diacetyllegionaminic acid from UDP-N,N'-diacetylbacillosamine in Legionella pneumophila.

Journal

Biochemistry 47:3272-82 (2008)
DOI:10.1021/bi702364s

Sequence

[lpn:lpg0752]

Reference

2 [PMID:19282391]

Authors

Schoenhofen IC, Vinogradov E, Whitfield DM, Brisson JR, Logan SM

Title

The CMP-legionaminic acid pathway in Campylobacter: biosynthesis involving novel GDP-linked precursors.

Journal

Glycobiology 19:715-25 (2009)
DOI:10.1093/glycob/cwp039

Sequence

[cje:Cj1327]

Other DBs

ExplorEnz - The Enzyme Database:

2.5.1.101

IUBMB Enzyme Nomenclature:

2.5.1.101

ExPASy - ENZYME nomenclature database:

2.5.1.101

BRENDA, the Enzyme Database:

2.5.1.101

LinkDB

DBGET integrated database retrieval system