KEGG   ENZYME: 2.5.1.73
Entry
EC 2.5.1.73                 Enzyme                                 
Name
O-phospho-L-seryl-tRNA:Cys-tRNA synthase;
SepCysS;
Sep-tRNA:Cys-tRNA synthase
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
Sysname
O-phospho-L-seryl-tRNACys:hydrogen sulfide 2-aminopropanoate transferase
Reaction(IUBMB)
O-phospho-L-seryl-tRNACys + sulfide = L-cysteinyl-tRNACys + phosphate [RN:R08577]
Reaction(KEGG)
R08577
Substrate
O-phospho-L-seryl-tRNACys;
sulfide [CPD:C00283]
Product
L-cysteinyl-tRNA(Cys) [CPD:C03125];
phosphate [CPD:C00009]
Comment
In organisms like Archaeoglobus fulgidus lacking EC 6.1.1.16 (cysteine---tRNA ligase) for the direct Cys-tRNACys formation, Cys-tRNACys is produced by an indirect pathway, in which EC 6.1.1.27 (O-phosphoseryl-tRNA ligase) ligates O-phosphoserine to tRNACys, and EC 2.5.1.73 converts the produced O-phospho-L-seryl-tRNACys to Cys-tRNACys. The SepRS/SepCysS pathway is the sole route for cysteine biosynthesis in the organism [1]. Methanosarcina mazei can use both pathways, the direct route using EC 6.1.1.16 (cysteine---tRNA ligase) and the indirect pathway with EC 6.1.1.27 (O-phosphoseryl-tRNA ligase) and EC 2.5.1.73 [2].
History
EC 2.5.1.73 created 2009
Pathway
ec00970  Aminoacyl-tRNA biosynthesis
Orthology
K06868  Sep-tRNA:Cys-tRNA synthetase
Genes
MJAMJ_1678
MFEMefer_0975
MVUMetvu_1276
MFSMFS40622_0574
MIFMetin_0086
MJHJH146_1495
MESGMLAUSG7_1199
MESAMLASG1_0578
MIGMetig_1345
MMPMMP1240
MMQMmarC5_0351
MMXMmarC6_1433
MMZMmarC7_0486
MMDGYY_07035
MMAKMMKA1_14150(pscS) MMKA1_14320(pscS) MMKA1_14490(pscS) MMKA1_14660(pscS)
MMAOMMOS7_13480(pscS)
MMADMMJJ_16050(pscS)
MAEMaeo_0681
MVNMevan_0554
MVOMvol_0651
MOKMetok_0264
METFCFE53_03625(pscS)
MTHMTH_1067
MMGMTBMA_c14550(pscS)
METCMTCT_0968
MWOMWSIV6_1453
METEtca_01031(iscS)
METZMETMT2_1082
METKFVF72_06255(pscS)
MTHMFZP57_06960(pscS)
METJFZP68_03675(pscS)
MTEEMTTB_03670
MAROMarbSA_16340
MELMetbo_2382
MEWMSWAN_0264
METHMBMB1_0325
MFCBRM9_1944(pscS)
MFIDSM1535_0132
MCUBMCBB_0296
MSUBBK009_11800
METNBK008_07435
METTCIT01_03215(pscS)
METOCIT02_05225(pscS)
MEMEHYG87_04490(pscS)
MFVMfer_0642
MKAMK0229
AFUAF_0028 AF_0181
AFGAFULGI_00000260
APOArcpr_0056
AVEArcve_1020
ASTAsulf_02152
FPLFerp_2023
GACGACE_1091
GAHGAH_00355 GAH_02061
MBAMbar_A1638
MBYMSBRM_2913
MBWMSBRW_3093
MBARMSBR2_2871
MBAKMSBR3_0854 MSBR3_1924
MACMA_0722
MMAMM_1881
MMAZMmTuc01_1956
MMJMSMAS_2806
MMACMSMAC_1632
MVCMSVAZ_1419 MSVAZ_2569
MEKMSKOL_1368 MSKOL_2576
MLSMSLAZ_0389
METMMSMTP_2705
MEFMSWH1_2638
MEQMSWHS_2397
MSJMSSAC_0667
MSZMSSIH_0610
MSWMSSIT_0641
MTHRMSTHT_0797 MSTHT_1396
MTHEMSTHC_1892 MSTHC_2472
MHORMSHOH_3505
MFZAOB57_006010(pscS)
MBUMbur_0796 Mbur_1440
MMETMCMEM_0673 MCMEM_0928 MCMEM_1925
MELOJ7W08_01470(pscS) J7W08_03050(pscS) J7W08_08590(pscS)
MMHMmah_0384 Mmah_0699 Mmah_1745
MHAZBHR79_01585 BHR79_04975
MEVMetev_1356
MZHMzhil_1846
MPYMpsy_1019 Mpsy_2114
MZIHWN40_11620(pscS)
MHZMetho_0600 Metho_2240
MTPMthe_0102
MCJMCON_0570(pscS)
MHIMhar_0427
MHUMhun_0071 Mhun_2180
MRTJKHC33_04530(pscS) KHC33_15315(pscS)
MLAMlab_0348 Mlab_1214
MEMMemar_1614
MBGBN140_0832
MEMAMMAB1_1094
MCHKMchiMG62_18000
MPIMpet_1028
MBNMboo_1050 Mboo_2045
MFOMetfor_1987 Metfor_2473
MPLMpal_1163 Mpal_1209
MPDMCP_0857
MEZMtc_1599 Mtc_2256
RCIRCIX1553 RCIX2270
MELUMTLP_12980
BARCAOA65_1061
BARBAOA66_0045
 » show all
Reference
1  [PMID:17512006]
  Authors
Fukunaga R, Yokoyama S
  Title
Structural insights into the second step of RNA-dependent cysteine biosynthesis in archaea: crystal structure of Sep-tRNA:Cys-tRNA synthase from Archaeoglobus fulgidus.
  Journal
J Mol Biol 370:128-41 (2007)
DOI:10.1016/j.jmb.2007.04.050
  Sequence
[afu:AF_0028]
Reference
2  [PMID:18559341]
  Authors
Hauenstein SI, Perona JJ
  Title
Redundant synthesis of cysteinyl-tRNACys in Methanosarcina mazei.
  Journal
J Biol Chem 283:22007-17 (2008)
DOI:10.1074/jbc.M801839200
Reference
3  [PMID:17142313]
  Authors
Yuan J, Palioura S, Salazar JC, Su D, O'Donoghue P, Hohn MJ, Cardoso AM, Whitman WB, Soll D
  Title
RNA-dependent conversion of phosphoserine forms selenocysteine in eukaryotes and  archaea.
  Journal
Proc Natl Acad Sci U S A 103:18923-7 (2006)
DOI:10.1073/pnas.0609703104
Other DBs
ExplorEnz - The Enzyme Database: 2.5.1.73
IUBMB Enzyme Nomenclature: 2.5.1.73
ExPASy - ENZYME nomenclature database: 2.5.1.73
BRENDA, the Enzyme Database: 2.5.1.73
CAS: 1239229-21-0
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