KEGG   ENZYME: 2.6.1.121
Entry
EC 2.6.1.121                Enzyme                                 
Name
8-amino-7-oxononanoate carboxylating dehydrogenase;
bioU (gene name)
Class
Transferases;
Transferring nitrogenous groups;
Transaminases
Sysname
(8S)-8-amino-7-oxononanoate:[protein]-L-lysine aminotransferase (N-carboxylating)
Reaction(IUBMB)
(8S)-8-amino-7-oxononanoate + [protein]-L-lysine + CO2 = (7R,8S)-8-amino-7-(carboxyamino)nonanoate + [protein]-(S)-2-amino-6-oxohexanoate (overall reaction) [RN:R12948];
(1a) (8S)-8-amino-7-oxononanoate + [protein]-L-lysine + NAD(P)H + H+ = [protein]-N6-[(2S,3R)-2-amino-8-carboxyoctan-3-yl]-L-lysine + H2O + NAD(P)+ [RN:R12949 R12952];
(1b) [protein]-N6-[(2S,3R)-2-amino-8-carboxyoctan-3-yl]-L-lysine + CO2 + H2O + NAD(P)+ = (7R,8S)-8-amino-7-(carboxyamino)nonanoate + [protein]-(S)-2-amino-6-oxohexanoate + NAD(P)H + H+ [RN:R12950 R12953]
Reaction(KEGG)
Substrate
(8S)-8-amino-7-oxononanoate [CPD:C22463];
[protein]-L-lysine [CPD:C02188];
CO2 [CPD:C00011];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080];
[protein]-N6-[(2S,3R)-2-amino-8-carboxyoctan-3-yl]-L-lysine [CPD:C22465];
H2O [CPD:C00001];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006]
Product
(7R,8S)-8-amino-7-(carboxyamino)nonanoate [CPD:C22464];
[protein]-(S)-2-amino-6-oxohexanoate [CPD:C03531];
[protein]-N6-[(2S,3R)-2-amino-8-carboxyoctan-3-yl]-L-lysine [CPD:C22465];
H2O [CPD:C00001];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
The enzyme, which participates in biotin biosynthesis, is found in haloarchaea and some cyanobacteria. It forms a conjugant between (7R,8S)-8-amino-7-oxononanoate and an internal lysine residue and catalyses multiple reactions, including a reduction, a carboxylation of the epsilon-amino group of the lysine residue, and an oxidative cleavage of the conjugate to release (7R,8S)-8-amino-7-(carboxyamino)nonanoate. During this process the lysine residue serves as an amino donor and is converted to (S)-2-amino-6-oxohexanoate, resulting in inactivation of the enzyme following a single turnover. cf. EC 2.6.1.105, lysine---8-amino-7-oxononanoate transaminase.
History
EC 2.6.1.121 created 2021
Pathway
ec00780  Biotin metabolism
ec01100  Metabolic pathways
Orthology
K25570  8-amino-7-oxononanoate carboxylating dehydrogenase
Genes
SYNslr0355
SYZMYO_122290
SYYSYNGTS_2205(slr0355)
SYTSYNGTI_2204(slr0355)
SYSSYNPCCN_2203(slr0355)
SYQSYNPCCP_2203(slr0355)
SYJD082_19980
SYOC7I86_11525
SYPSYNPCC7002_A2217
SYNPSyn7502_03281
SYVAWQ23_11065
SYLAWQ21_11220
DSLDacsa_3533
AMRAM1_3508
WNAKA717_10265
LEPLepto7376_1721
LSCKIK02_03870
LSKJ5X98_00925
HHGXM38_052210(gdhA)
PSEUPse7367_1578
PSERABRG53_2444
GENGM3709_3187
GEEGM3708_446
CHONNIES4102_13170
MARMAE_52130
MPKVL20_1628
MIQB5D77_06185
MVZmyaer102_24930
CSNCyast_1436
CYLAA637_13255
HAOPCC7418_1108
ENNFRE64_06820
CYTcce_3615
CWACwatDRAFT_0322
CYPPCC8801_2161
CYHCyan8802_2223
CYCPCC7424_0836
CYJCyan7822_5128
CYNCyan7425_5085
TERTery_2576
ARPNIES39_K03140
PAGHNIES204_11170
PPSUNO713_05154
OXYHCG48_24655
LFSHFV01_01590
MICMic7113_0861
GEIGEI7407_0392
OACOscil6304_0680
MPROBJP34_28010
PHORJWS08_07230
PYHNEA10_03540
CEPCri9333_4105
GVIgll0528
GLJGKIL_1220
GMLISF26_16410
ANAalr2440
NPUNpun_F0114
NOSNos7107_1213
NOPNos7524_4563
NONNOS3756_05190
NFLCOO91_04968
NOECLI64_13535
NSHGXM_05630
NEDHUN01_28990
AVAAva_0372
NAZAazo_1966
ANBANA_C13203
ACYAnacy_4347
AWAAA650_04800
ANNEH233_06115
CSGCylst_5857
RSINB6N60_03227
CALOCal7507_6097
CALTCal6303_1482
CALHIJ00_13450
CALNNIES2098_60510
FISFIS3754_36280
NSPBMF81_02251
NSPHBDGGKGIB_01917
DOUBMF77_03299
DFSHGD76_20955
DHTNG743_17340
CCURIAR63_11295
CRKL3I90_02060
AFLOHEQ12_06040
TOQHCG51_05015
NCNBZZ01_27820
PLPPle7327_3173
SCSSta7437_1699
STANSTA3757_22720
CERRGRSB_1164
HAADMW046_12510
SALIL593_14820
SAIMK0C01_06305
HMArrnAC0884(gdhA3)
HHIHAH_1521
HHNHISP_07770
HABSG26_15600
HTABVU17_07395
HALJG9465_11560
HSINKDQ40_05825
NPHNP_4234A
NHOHWV23_16150
NSALHWV07_00995
HMUHmuk_2295
HALZE5139_14610
HALLLC1Hm_1523
HAZPGBQ70_14625
HALIBV210_17475
HRRHZS55_15520
HPELHZS54_18570
HLTI7X12_20070
HDOMUK72_10445
HMEHFX_5078
HLSKU306_16045
HBOHbor_32760
HTUHtur_1909
HDABB347_05620
HSALJMJ58_19845
HLOJ0X27_00235
HAKZJ0X25_16395
NATNJ7G_0817
NPENatpe_1551
NVRFEJ81_07980
NPLFGF80_02630
NAYHYG81_00655
HALYHYG82_03420
HJTDVR14_17310 DVR14_21440
NGENatgr_2204
NOUNatoc_4148
HLRHALLA_13585
NAJB1756_14330
NBGDV706_13590
NASGCU68_14200
 » show all
Reference
1  [PMID:32042199]
  Authors
Sakaki K, Ohishi K, Shimizu T, Kobayashi I, Mori N, Matsuda K, Tomita T, Watanabe H, Tanaka K, Kuzuyama T, Nishiyama M.
  Title
A suicide enzyme catalyzes multiple reactions for biotin biosynthesis in cyanobacteria.
  Journal
Nat Chem Biol 16:415-422 (2020)
DOI:10.1038/s41589-019-0461-9
  Sequence
[syn:slr0355]
Other DBs
ExplorEnz - The Enzyme Database: 2.6.1.121
IUBMB Enzyme Nomenclature: 2.6.1.121
ExPASy - ENZYME nomenclature database: 2.6.1.121
BRENDA, the Enzyme Database: 2.6.1.121
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