KEGG ENZYME: 2.6.1.86

ENZYME: 2.6.1.86

Entry

EC 2.6.1.86                 Enzyme

Name

2-amino-4-deoxychorismate synthase;
ADIC synthase;
2-amino-2-deoxyisochorismate synthase;
SgcD

Class

Transferases;
Transferring nitrogenous groups;
Transaminases

Sysname

(2S)-2-amino-4-deoxychorismate:2-oxoglutarate aminotransferase

Reaction(IUBMB)

(2S)-2-amino-4-deoxychorismate + L-glutamate = chorismate + L-glutamine [RN:R08956]

Reaction(KEGG)

R08956

Substrate

(2S)-2-amino-4-deoxychorismate [CPD:C18054];
L-glutamate [CPD:C00025]

Product

chorismate [CPD:C00251];
L-glutamine [CPD:C00064]

Comment

Requires Mg2+. The reaction occurs in the reverse direction to that shown above. In contrast to most anthranilate-synthase I (ASI) homologues, this enzyme is not inhibited by tryptophan. In Streptomyces globisporus, the sequential action of this enzyme and EC 1.3.8.16, 2-amino-4-deoxychorismate dehydrogenase, leads to the formation of the benzoxazolinate moiety of the enediyne antitumour antibiotic C-1027 [1,2]. In certain Pseudomonads the enzyme participates in the biosynthesis of phenazine, a precursor for several compounds with antibiotic activity [3,4].

History

EC 2.6.1.86 created 2008

Pathway

ec00405

Phenazine biosynthesis

ec01059

Biosynthesis of enediyne antibiotics

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K13063

2-amino-4-deoxychorismate synthase

K20159

2-amino-4-deoxychorismate synthase

K21175

2-amino-4-deoxychorismate synthase, glutamine amidotransferase component

Genes

KOC:	AB185_20690

KGR:	JJJ10_13555

KPAS:

LUW96_21810

KLM:	BWI76_15100

SFW:	WN53_03385

ECA:	ECA2701(ehpC)

PATR:

EV46_13195

PATO:

GZ59_18590(ehpC)

PPAV:

LOZ86_13695

PACB:

M9782_10870

XDO:	XDD1_2436(phzE)

XPO:	XPG1_2291(tomD)

XHO:	A9255_10780

LAB:	LA76x_3333

LAQ:	GLA29479_2366(phzE)

DYG:	DYGSA30_26140(phzE1)

VRU:	RND59_07995

PHOT:

L4174_007380

PAE:	PA1903(phzE2) PA4214(phzE1)

PAEV:

N297_1963 N297_4346

PAEI:

N296_1963 N296_4346

PAU:	PA14_09440(phzE1) PA14_39910(phzE2)

PAP:	PSPA7_0885 PSPA7_3381

PAG:	PLES_07131(phzE1) PLES_34211(phzE2)

PAF:	PAM18_0723(phzE1) PAM18_3139(phzE2)

PNC:	NCGM2_2820(phzE2) NCGM2_5459(phzE2)

PAEB:

NCGM1900_0745(phzE2) NCGM1900_4586(phzE2)

PDK:	PADK2_03600 PADK2_16135

PSG:	G655_03635 G655_15600

PRP:	M062_22200

PAEP:

PA1S_03750 PA1S_16425

PAER:

PA1R_gp2105 PA1R_gp5532

PAEM:

U769_03780 U769_16140

PAEL:

T223_17485

PAES:

SCV20265_0747 SCV20265_3492

PAEU:

BN889_07250(phzE1_9)

PAEG:

AI22_00160 AI22_17565

PAEC:

M802_1961 M802_4344

PAEO:

M801_1962 M801_4212

SECH:

B18_04215 B18_21405

PPAA:

B7D75_03680 B7D75_15425

PAST:

N015_20080

PFB:

VO64_0338

POI:	BOP93_12300

PCH:	EY04_26290

PCP:	JM49_05455

PCHP:

C4K32_5263

PSET:

THL1_3622

PYM:	AK972_2557

PSEP:

C4K39_3097

PPII:

QL104_15635

PKV:	QYQ93_14705

PBAM:

NUH87_15865

BUU:	WS70_27185

BUR:	Bcep18194_B1570

BGL:	bglu_2g09260

BGU:

KS03_4991

TVL:	FAZ95_31785

RUG:	QC826_13635

BVR:

BVIR_2862

MAB:

MAB_0298

MMV:	MYCMA_0160

MABB:

MASS_0299

MABL:

MMASJCM_0295

MCHE:

BB28_01470

MIZ:	BAB75_02020

MSTE:

MSTE_00260

MSAO:

MYCSP_01300

MSAL:

DSM43276_00253(trpE_1)

CMV:	CMUST_14530

NBR:	O3I_033275

NTP:	CRH09_09655

NAH:	F5544_33405

RHOP:

D8W71_03295

RHOO:

RA302_31650

SCO:	SCO2117(SC6E10.11)

SALB:

XNR_0598 XNR_4762

SMA:	SAVERM_6084(phzE)

SGR:

SGR_5385

SGB:	WQO_08390 WQO_33795 WQO_33800

SFI:	SFUL_1692 SFUL_3323 SFUL_5261

SANL:

KZO11_08155

SGRF:

SGFS_036800 SGFS_084800

SCYE:

R2B67_04375 R2B67_27930

SCB:	SCAB_12051 SCAB_67631(trpE)

SSX:	SACTE_1549 SACTE_1751

SVL:	Strvi_7202

SHY:

SHJG_3601

SHO:	SHJGH_3366

SRC:	M271_35750

SMAL:

SMALA_2052

SFA:

Sfla_4700

SBH:

SBI_07844

SVE:

SVEN_1778

SDV:	BN159_6319(phzB)

SALS:

SLNWT_4920 SLNWT_5765

STRP:

F750_1981

SCI:	B446_11110

SLV:	SLIV_27120(phzB)

SGU:	SGLAU_09650 SGLAU_17225(phzB)

SVT:	SVTN_10295

STRE:

GZL_06398

SCW:	TU94_09180

SLD:

T261_6049

SLC:	SL103_08680

SXI:	SXIM_10580

STRM:

M444_10845 M444_18415

STRC:

AA958_06685 AA958_30775

SAMB:

SAM23877_2213(phzB)

SPRI:

SPRI_5382

SCZ:	ABE83_19215 ABE83_26420 ABE83_27390

SCX:	AS200_32305

SRW:	TUE45_02649(trpE_2)

STRF:

ASR50_10340

SLE:	sle_50210(sle_50210) sle_57420(sle_57420)

SRN:	A4G23_01307(trpG) A4G23_05416(trpE_2)

SPAV:

Spa2297_08695

STRT:

A8713_08010 A8713_24030

SCLF:

BB341_21450

SGS:	AVL59_37600

STSI:

A4E84_10935

SKY:	D0C37_25690

SALW:

CP975_09305

SGM:	GCM10017557_24340(phzE1) GCM10017557_60800

SCAD:

DN051_27795 DN051_33895

SPHW:

NFX46_21440 NFX46_31380

SLS:	SLINC_2449 SLINC_5461

SNR:	SNOUR_29770

SALL:

SAZ_14330

SPLU:

LK06_007460 LK06_026285

STRD:

NI25_24445 NI25_28590

SNW:	BBN63_05565 BBN63_25995

SAUO:

BV401_13735

SSIA:

A7J05_26290

SVU:	B1H20_07495

SPUN:

BFF78_15755 BFF78_30585

SGV:	B1H19_12510

SLAU:

SLA_1712

SALF:

SMD44_02324(phzE)

SALJ:

SMD11_1728 SMD11_3252 SMD11_4937(phzE)

SLX:	SLAV_26615(phnA)

STRO:

STRMOE7_11390

SFK:

KY5_2100

SGE:	DWG14_06212(trpE_1)

SRJ:

SRO_5342

SLK:	SLUN_10395

SDX:	C4B68_29570

SGD:	ELQ87_28725

SQZ:	FQU76_00260 FQU76_07185 FQU76_33480

SCYA:

EJ357_12420

SAST:

CD934_07150 CD934_24910

SNQ:	CP978_10230

STIR:

DDW44_03905 DDW44_31310 DDW44_31315

SKA:	CP970_32080

SGZ:	C0216_00430 C0216_23630

SVN:	CP980_14885 CP980_24250

SNK:	CP967_25455

SHAW:

CEB94_11480 CEB94_18880

SRK:	FGW37_08910 FGW37_24785

SFIC:

EIZ62_24520

SGAL:

CP966_09435

SSPO:

DDQ41_03275

SVR:	CP971_17415 CP971_24495

SPAD:

DVK44_06530 DVK44_32310

SFY:	GFH48_29340

SAQU:

EJC51_01045 EJC51_14230

SGF:	HEP81_05649

SCAV:

CVT27_07280 CVT27_15335

SSEO:

D0Z67_01710 D0Z67_07430

SBY:	H7H31_26910

SRIM:

CP984_30290

SSUB:

CP968_24085

SPHV:

F9278_10925

SCIN:

CP977_09525 CP977_15860

SFUG:

CNQ36_09440

SPAC:

B1H29_26765

STSU:

B7R87_07175

SCHA:

CP983_31690

SGX:	H4W23_10720

SCOE:

CP976_12245

SBAT:

G4Z16_26055

SSPB:

CP982_12420 CP982_28275

SNF:	JYK04_02801(trpE_2)

SPLA:

CP981_10505

SBRO:

GQF42_13570

STUI:

GCM10017668_17660

SATA:

C5746_11605

SCHF:

IPT68_09945

SHUN:

DWB77_04282(phnA) DWB77_05612(trpE_1)

SHAR:

HUT13_06035

SCYG:

S1361_11655(trpE2)

SFEU:

IM697_11280

SLIA:

HA039_25480 HA039_29905

SDW:	K7C20_10060

SVD:	CP969_11695

SCAL:

I6J39_07700

SAUH:

SU9_008485

SFB:	CP974_07175 CP974_28835

SMOB:

J7W19_08350

SPRA:

CP972_09725

SPEU:

CGZ69_09165

SROI:

IAG44_13940 IAG44_29595 IAG44_38340

SXN:	IAG42_26030

SGJ:	IAG43_07985

SHK:	J2N69_09115

SANU:

K7396_26350

STRY:

EQG64_07380 EQG64_16155

SDD:	D9753_26095

SACT:

DMT42_10600

SMAO:

CAG99_00485 CAG99_15055 CAG99_22525

SGOB:

test1122_03660

SINE:

KI385_13220

SLF:	JEQ17_03400 JEQ17_33870

SDEC:

L3078_12445

SAOV:

G3H79_27475

SDUR:

M4V62_30495

SYAN:

NRK68_09625

SRUG:

F0345_06290

SAKB:

K1J60_33075

STAA:

LDH80_28645

SNIG:

HEK616_13800 HEK616_55580(phzE1)

SVIO:

HWN34_24055

SCAE:

IHE65_08530 IHE65_33200

STUB:

MMF93_08700

SSPN:

LXH13_11150

SCIR:

STRCI_002330

SXT:	KPP03845_102364(trpE_2)

SLON:

LGI35_14515

STUD:

STRTU_005063 STRTU_005284

STEE:

F3L20_15975 F3L20_24160

SENG:

OJ254_17515

SGK:	PET44_09465 PET44_17170

SANT:

QR300_31300

SYUN:

MOV08_29790

SARG:

HKX69_25340

SJN:	RI060_31360

SCYN:

N8I84_11805 N8I84_14855 N8I84_30910

SOV:	QZH56_26835

SFIY:

F0344_27775

SHAU:

K9S39_32480 K9S39_36115

SKG:	KJK29_05875 KJK29_28315

SCOA:

QU709_14205 QU709_32190

SLAI:

P8A22_28645 P8A22_34750 P8A22_34810

SROC:

RGF97_25380

SFP:	QUY26_29530

SCHG:

NRO40_07220 NRO40_23345 NRO40_27015

STTN:

BSL84_09305

SINN:

ABB07_25520 ABB07_27760

STRZ:

OYE22_26220

SCAY:

PYS65_26855

KBU:	Q4V64_13225 Q4V64_42960

SARM:

DVA86_08975

SPRF:

K4G22_02685 K4G22_05380

SLUO:

PS467_12335

STPB:

QR97_29080

SRPA:

HEP86_11790

SMIB:

SMIR_28355

SCAJ:

O1G22_30620 O1G22_43770

STRW:

QHG49_25565

STRL:

HEP84_16565

STSD:

HEP87_16660

SRPZ:

HEP85_11865

SCT:	SCAT_1246(phzB)

SCY:	SCATT_12460

KSK:	KSE_21580 KSE_58290 KSE_59080

KAB:	B7C62_07380

KAU:	B6264_19060

KIT:	CFP65_1906 CFP65_3610

KIS:	HUT16_09245

KCH:	O1G21_10000 O1G21_27130 O1G21_27715

STRI:

C7M71_006375 C7M71_014735

YIA:	LO772_24360

STRH:

GXP74_10115

ABRY:

NYE86_09305 NYE86_21260 NYE86_22020

AGLA:

OIE69_28705

MTS:

MTES_0388

MIM:	AKG07_02285

MIO:	AOA12_15035

MIX:	AB663_002144

MIP:	AXH82_13275

MCW:	A8L33_08180

MPAL:

BO218_15790

MIH:	BJP65_03865

MICR:

BMW26_06855

MAUR:

BOH66_12155

MHOS:

CXR34_12920

MFOL:

DXT68_06730

MOO:	BWL13_02068(trpE)

MLV:	CVS47_00972(trpE)

MWA:	E4K62_05860

MPRT:

ET475_00770

MSED:

E3O41_04100

MOY:	CVS54_02214(trpE)

MSF:	IT882_04630

MCAW:

F6J84_10575

MLZ:	F6J85_05585

MRN:	K8F61_07210

MICS:

C1N74_11315

MBIN:

LXM64_11335

MLIQ:

NMQ05_10630

MTEC:

OAU46_10360

MPAO:

IZR02_06620

MAZA:

NFX31_10730

MNF:	JSY13_04580

MSUW:

GCM10025863_12410(phzE1)

MARK:

QUC20_11215

MRG:	SM116_13660

MINV:

T9R20_11640

MEST:

PTQ19_05735

MOJ:	D7D94_06505

MPLN:

VC184_11005

MLIY:

RYJ27_08100

MICE:

EHF32_01590

MAUG:

KV397_09665

MSUF:

KV394_12230

MHOT:

PU630_05070

MGAB:

KV396_12995

MCHN:

HCR76_06035

RTX:	TI83_09780

RTC:	APU90_07285

RTN:	A6122_2322

RRY:	C1O28_10550

RIA:	C7V51_15390

RFS:	C1I64_12250

RTE:	GSU10_03085

FSB:	GCM10025867_11420(phzE1)

AGY:	ATC03_07245

AGM:	DCE93_03990

AGF:	ET445_09520

ALAV:

MTO99_07870

AIL:	FLP10_14575

HUW:	FPZ11_06840

GRY:	D7I44_05760

PLAP:

EAO79_11405

HOM:	OF852_10720

BHQ:	BRM3_04145

KSE:	Ksed_06140

CPRT:

FIC82_000180

SANW:

G7063_02290

CEZ:	CBP52_12010

CPAL:

F1D97_01430

SERJ:

SGUI_2558

SERW:

FY030_13650

ORN:	DV701_08890

ORZ:	FNH13_03665

ORD:	L0A91_10350

OCP:	NF557_15150

OFA:	NF556_02750

TFA:	BW733_09555

NCA:

Noca_4529

NDK:	I601_1070(phnA_1)

NOY:	EXE57_12930

NOI:	FCL41_00890

NOO:	FE634_20225

NDP:	E2C04_01520

NSN:	EXE58_09250

NBE:	Back2_19310

NANO:

G5V58_23605

NMES:

H9L09_08155

NARO:

CFH99_01700

NAQU:

ENKNEFLB_04326(phnA_2)

NRO:	K8W59_00900

NOQ:	LN652_11950

NCQ:	K6T13_16420

NPC:	KUV85_12570

NPS:	KRR39_21195

NMAR:

HPC71_20000

NFC:	KG111_00575

NRN:	SHK17_19950

NBI:	SHK19_00695

PSIM:

KR76_01360

AER:	AERYTH_00460

AEZ:	C3E78_17405

AEB:	C6I20_16275

AEF:	GEV26_17160

ASEZ:

H9L21_14320

ADB:	NP095_14280

AWN:	NQV15_17450

MUZ:	H4N58_18775

SGRG:

L0C25_08785

NBT:	KLP28_05890

KRI:	OX958_35225

THAO:

NI17_009040

TALX:

FOF52_14765

NDA:

Ndas_5031

NEX:	NE857_12065

NAKE:

KGD83_26650

NCG:	KGD84_13380

STRR:

EKD16_18445(phnA)

NOA:	BKM31_19745

MHAI:

OHB01_22670

FAL:

FRAAL3440

FRE:	Franean1_3620 Franean1_3878

KRA:

Krad_1199

SACG:

FDZ84_16250

AMYC:

CU254_36715

APRE:

CNX65_15960

SSYI:

EKG83_21245

KAL:

KALB_2053

KUT:	JJ691_21750 JJ691_54330

KPHY:

AOZ06_16445 AOZ06_18055 AOZ06_22500 AOZ06_25090

ALO:

CRK58741

ACTU:

Actkin_02797(menF)

STP:	Strop_2960

SAQ:

Sare_3182

MAU:	Micau_4172

MIL:

ML5_4130

MICB:

MicB006_2906 MicB006_3169

MTUA:

CSH63_03790

MICH:

FJK98_01245 FJK98_22465

MTEM:

GCE86_15205

MCAB:

HXZ27_21235

MSAG:

GCM10017556_03700(phzE1) GCM10017556_11920(phzE1)

VMA:	VAB18032_25130

VER:	HUT12_21420

MENO:

Jiend_49390(phzE1)

MCHL:

PVK74_09485 PVK74_10905

MFEU:

H1D33_26465

MPRN:

Q3V37_17270

MHAW:

RMN56_22485

MICF:

QQG74_21885

ASE:	ACPL_1962(phzE)

AMS:	AMIS_17120

ACTN:

L083_2374 L083_5379(phzE)

ACTS:

ACWT_1840

AIH:	Aiant_63010(phzE1)

ACTR:

Asp14428_22430(phzE1)

ACTL:

L3i22_019330(phzE1)

ASIC:

Q0Z83_069890

AOU:	ACTOB_001912

ACTE:

ACTI_38870(phzE1)

AFS:

AFR_11460

PLK:	CIK06_19030 CIK06_19035

PLAB:

C6361_09300

PLAT:

C6W10_05810

PFLA:

Pflav_059820(phzE1)

PSUU:

Psuf_006100(phzE1_1) Psuf_020570(phzE1_2) Psuf_091170(phzE1_3)

PRY:	Prubr_00870(phzE1_1) Prubr_64690(phzE1_2)

CATI:

CS0771_71150(phzE1)

DVC:	Dvina_11065 Dvina_40335

DFU:	Dfulv_10425 Dfulv_24070 Dfulv_30535 Dfulv_33865

DROS:

Drose_10345 Drose_13370 Drose_22880

DAUR:

Daura_10410

DMAT:

Dmats_35195

DACT:

KZZ52_10335 KZZ52_19485

CCAZ:

COUCH_28315

CAI:

Caci_0239

GLY:	K3N28_00675

ACTC:

CHIBA101_0088

AEY:	CDG81_16845

MICA:

P0L94_07595

» show all

Reference

1 [PMID:18182490]

Authors

Van Lanen SG, Lin S, Shen B

Title

Biosynthesis of the enediyne antitumor antibiotic C-1027 involves a new branching point in chorismate metabolism.

Journal

Proc Natl Acad Sci U S A 105:494-9 (2008)
DOI:10.1073/pnas.0708750105

Sequence

[ag:AAL06664]

Reference

Authors

Yu L, Mah S, Otani T, Dedon P.

Title

The benzoxazolinate of C-1027 confers intercalative DNA binding.

Journal

J Am Chem Soc 117:8877-8878 (1995)

Reference

3 [PMID:11562236]

Authors

McDonald M, Mavrodi DV, Thomashow LS, Floss HG

Title

Phenazine biosynthesis in Pseudomonas fluorescens: branchpoint from the primary shikimate biosynthetic pathway and role of phenazine-1,6-dicarboxylic acid.

Journal

J Am Chem Soc 123:9459-60 (2001)
DOI:10.1021/ja011243+

Sequence

[ag:AAC18904]

Reference

4 [PMID:15008629]

Authors

Laursen JB, Nielsen J.

Title

Phenazine natural products: biosynthesis, synthetic analogues, and biological activity.

Journal

Chem Rev 104:1663-86 (2004)
DOI:10.1021/cr020473j