KEGG   ENZYME: 2.8.4.1
Entry
EC 2.8.4.1                  Enzyme                                 
Name
coenzyme-B sulfoethylthiotransferase;
methyl-CoM reductase;
methyl coenzyme M reductase
Class
Transferases;
Transferring sulfur-containing groups;
Transferring alkylthio groups
Sysname
methyl-CoM:CoB S-(2-sulfoethyl)thiotransferase
Reaction(IUBMB)
methyl-CoM + CoB = CoM-S-S-CoB + methane [RN:R04541]
Reaction(KEGG)
R04541
Substrate
methyl-CoM [CPD:C03920];
CoB [CPD:C04628]
Product
CoM-S-S-CoB [CPD:C04832];
methane [CPD:C01438]
Comment
This enzyme catalyses the final step in methanogenesis, the biological production of methane. This important anaerobic process is carried out only by methanogenic archaea. The enzyme can also function in reverse, for anaerobic oxidation of methane.The enzyme requires the hydroporphinoid nickel complex coenzyme F430. Highly specific for coenzyme B with a heptanoyl chain; ethyl CoM and difluoromethyl CoM are poor substrates. The sulfide sulfur can be replaced by selenium but not by oxygen.
History
EC 2.8.4.1 created 2001, modified 2011
Pathway
ec00680  Methane metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K00399  methyl-coenzyme M reductase alpha subunit
K00401  methyl-coenzyme M reductase beta subunit
K00402  methyl-coenzyme M reductase gamma subunit
Genes
MJAMJ_0081 MJ_0082 MJ_0083 MJ_0842 MJ_0845 MJ_0846
MFEMefer_0934 Mefer_0937 Mefer_0938
MVUMetvu_0145 Metvu_0148 Metvu_0149 Metvu_1192 Metvu_1193 Metvu_1194
MFSMFS40622_0424 MFS40622_0425 MFS40622_0426 MFS40622_1315 MFS40622_1316 MFS40622_1319
MIFMetin_0281 Metin_0282 Metin_0285
MJHJH146_0503 JH146_0504 JH146_0505 JH146_1056 JH146_1057 JH146_1060
MESGMLAUSG7_0353(mcrA) MLAUSG7_0354(mcrG) MLAUSG7_0357(mcrB)
MESAMLASG1_1532(mcrA) MLASG1_1533(mcrG) MLASG1_1536(mcrB)
MIGMetig_0442 Metig_0444 Metig_0445 Metig_1233 Metig_1236 Metig_1237
MMPMMP1555(mcrB) MMP1558(mcrG) MMP1559(mcrA)
MMQMmarC5_0017 MmarC5_0018 MmarC5_0021
MMXMmarC6_1111 MmarC6_1112 MmarC6_1115
MMZMmarC7_0802 MmarC7_0805 MmarC7_0806
MMDGYY_08625 GYY_08640 GYY_08645
MMAKMMKA1_17850(mcrB) MMKA1_17880(mcrG) MMKA1_17890(mcrA)
MMAOMMOS7_16670(mcrB) MMOS7_16700(mcrG) MMOS7_16710(mcrA)
MMADMMJJ_12770(mcrA) MMJJ_12780(mcrG) MMJJ_12810(mcB)
MAEMaeo_1264 Maeo_1267 Maeo_1268
MVNMevan_0868 Mevan_0871 Mevan_0872
MVOMvol_1124 Mvol_1127 Mvol_1128
MOKMetok_0956 Metok_0957 Metok_0960
METFCFE53_01190(mcrA) CFE53_01195(mcrG) CFE53_01210(mcrB)
MTHMTH_1129 MTH_1130 MTH_1132 MTH_1164 MTH_1165 MTH_1168
MMGMTBMA_c15120(mrtA) MTBMA_c15130(mrtG) MTBMA_c15150(mrtB) MTBMA_c15480(mcrA) MTBMA_c15490(mcrG) MTBMA_c15520(mcrB)
MWOMWSIV6_1521(mrtA) MWSIV6_1522(mrtG) MWSIV6_1524(mrtB) MWSIV6_1556(mcrA) MWSIV6_1557(mcrG) MWSIV6_1560(mcrB)
MTEEMTTB_02540(mcrB) MTTB_02570(mcrG) MTTB_02580(mcrA)
METCMTCT_1022 MTCT_1023 MTCT_1025 MTCT_1058 MTCT_1059 MTCT_1062
METEtca_01085 tca_01086 tca_01088 tca_01121 tca_01122 tca_01125
METZMETMT2_1172 METMT2_1173 METMT2_1176
METKFVF72_05745(mcrB) FVF72_05760(mcrG) FVF72_05765(mcrA) FVF72_05925(mcrB) FVF72_05935(mcrG) FVF72_05940(mcrA)
MTHMFZP57_07280(mcrA) FZP57_07285(mcrG) FZP57_07295(mcrB) FZP57_07455(mcrA) FZP57_07460(mcrG) FZP57_07475(mcrB)
METJFZP68_03200(mcrB) FZP68_03215(mcrG) FZP68_03220(mcrA) FZP68_03380(mcrB) FZP68_03390(mcrG) FZP68_03395(mcrA)
MSTMsp_0318(mrtB) Msp_0320(mrtG) Msp_0321(mrtA)
METBAW729_05480 AW729_05485 AW729_05495
MRUmru_1924(mcrA) mru_1925(mcrG) mru_1928(mcrB)
MSIMsm_0902 Msm_0903 Msm_0905 Msm_1015 Msm_1016 Msm_1019
MEBAbm4_1526(mcrA) Abm4_1527(mcrG) Abm4_1530(mcrB)
MMILsm9_1353(mrtA) sm9_1354(mrtG) sm9_1356(mrtB) sm9_2027(mcrA) sm9_2028(mcrG) sm9_2031(mcrB)
MEYETL18_09320 TL18_09325 TL18_09340
MOLYLM1_1108 YLM1_1110 YLM1_1111 YLM1_1379 YLM1_1380 YLM1_1383
MAROMarbSA_15380 MarbSA_15410 MarbSA_15420
METVK4897_06370(mcrB) K4897_06385(mcrG) K4897_06390(mcrA)
MELMetbo_0349 Metbo_0352 Metbo_0353 Metbo_2278 Metbo_2280 Metbo_2281
MEWMSWAN_2056 MSWAN_2057 MSWAN_2060
METHMBMB1_1682(mcrA) MBMB1_1683(mcrG) MBMB1_1686(mcrB) MBMB1_1822(mrtB) MBMB1_1824(mrtG) MBMB1_1825(mrtA)
MFCBRM9_0935(mcrA) BRM9_0936(mcrG) BRM9_0939(mcrB) BRM9_2153(mrtB) BRM9_2155(mrtG) BRM9_2156(mrtA)
MFIDSM1535_0905(mcrA) DSM1535_0906(mcrG) DSM1535_0909(mcrB) DSM1535_1092(mrtB) DSM1535_1094(mrtG) DSM1535_1095(mrtA)
MCUBMCBB_1985(mcrA) MCBB_1986(mcrG) MCBB_1989(mcrB)
MSUBBK009_07405 BK009_07410 BK009_07425 BK009_08280 BK009_08290 BK009_08295
METNBK008_03060 BK008_03065 BK008_03080 BK008_03955 BK008_03965 BK008_03970
METTCIT01_01100(mcrB) CIT01_01110(mcrG) CIT01_01115(mcrA) CIT01_03630(mcrA) CIT01_03635(mcrG) CIT01_03650(mcrB)
METOCIT02_08450(mcrA) CIT02_08455(mcrG) CIT02_08465(mcrB) CIT02_09460(mcrB) CIT02_09475(mcrG) CIT02_09480(mcrA)
MEMEHYG87_00765(mcrB) HYG87_00775(mcrG) HYG87_00780(mcrA) HYG87_04985(mcrA) HYG87_04990(mcrG) HYG87_05005(mcrB)
MFVMfer_0731 Mfer_0733 Mfer_0734 Mfer_0784 Mfer_0785 Mfer_0788
MKAMK0651(mcrB) MK0654(mcrG) MK0655(mcrA)
MBAMbar_A0893 Mbar_A0894 Mbar_A0897
MBYMSBRM_0097 MSBRM_0100 MSBRM_0101
MBWMSBRW_0101 MSBRW_0104 MSBRW_0105
MBARMSBR2_2279 MSBR2_2280 MSBR2_2283
MBAKMSBR3_2340 MSBR3_2341 MSBR3_2344
MACMA_4546 MA_4547 MA_4550
MMAMM_1240 MM_1241 MM_1244
MMAZMmTuc01_1281 MmTuc01_1282 MmTuc01_1285
MMJMSMAS_0069 MSMAS_0072 MSMAS_0073
MMACMSMAC_0068 MSMAC_0071 MSMAC_0072
MVCMSVAZ_3413 MSVAZ_3414 MSVAZ_3417
MEKMSKOL_3374 MSKOL_3375 MSKOL_3378
MLSMSLAZ_0156 MSLAZ_0157 MSLAZ_0160
METMMSMTP_0096 MSMTP_0099 MSMTP_0100
MEFMSWH1_0085 MSWH1_0088 MSWH1_0089
MEQMSWHS_0088 MSWHS_0091 MSWHS_0092
MSJMSSAC_4251 MSSAC_4254 MSSAC_4255
MSZMSSIH_3755 MSSIH_3758 MSSIH_3759
MSWMSSIT_3834 MSSIT_3837 MSSIT_3838
MTHRMSTHT_1640 MSTHT_1643 MSTHT_1644
MTHEMSTHC_1645 MSTHC_1646 MSTHC_1649
MHORMSHOH_0080 MSHOH_0083 MSHOH_0084
MFZAOB57_007315(mcrB) AOB57_007330(mcrG) AOB57_007335(mcrA)
MBUMbur_2417(mcrA) Mbur_2418(mcrG) Mbur_2421(mcrB)
MMETMCMEM_0559 MCMEM_0560 MCMEM_0563
MELOJ7W08_00830(mcrA) J7W08_00835(mcrG) J7W08_00850(mcrB)
MMHMmah_0612 Mmah_0613 Mmah_0616
MHAZBHR79_00290 BHR79_00305 BHR79_00310
MEVMetev_0902 Metev_0903 Metev_0906
MZHMzhil_0853 Mzhil_0856 Mzhil_0857
MPYMpsy_0828 Mpsy_0829 Mpsy_0832
MZIHWN40_04500(mcrB) HWN40_04515(mcrG) HWN40_04520(mcrA)
MHZMetho_1666 Metho_1669 Metho_1670
MTPMthe_0569 Mthe_0570 Mthe_0572
MCJMCON_0759(mcrA) MCON_0760(mcrG) MCON_0762(mcrB)
MHIMhar_0495 Mhar_0497 Mhar_0498
MHUMhun_2144 Mhun_2147 Mhun_2148
MRTJKHC33_15110(mcrB) KHC33_15125(mcrG) KHC33_15130(mcrA)
MLAMlab_1066 Mlab_1067 Mlab_1068 Mlab_1561 Mlab_1562 Mlab_1565
MEMMemar_0375 Memar_0377 Memar_0378 Memar_0613 Memar_0614 Memar_0617
MBGBN140_1521(mcrA1) BN140_1522(mcrG1) BN140_1524(mcrB1) BN140_1734(mcrB3) BN140_1737(mcrG3) BN140_1738(mcrA3)
MEMAMMAB1_1952(mcrA) MMAB1_1953(mcrG) MMAB1_1956 MMAB1_2231(mcrB) MMAB1_2234(mcrG) MMAB1_2235(mcrA)
MCHKMchiMG62_05000(mcrB_1) MchiMG62_05020 MchiMG62_05030 MchiMG62_07420 MchiMG62_07430 MchiMG62_07460(mcrB_2)
MSUMOH143_08575(mcrB) OH143_08585(mcrG) OH143_08590(mcrA) OH143_09785(mcrA) OH143_09790(mcrG) OH143_09805(mcrB)
MPIMpet_1991 Mpet_1992 Mpet_1993 Mpet_2415 Mpet_2416 Mpet_2419
MENDL6E24_09485(mcrB) L6E24_09500(mcrG) L6E24_09505(mcrA)
MBNMboo_0582 Mboo_0583 Mboo_0586
MFOMetfor_0871 Metfor_0873 Metfor_0874 Metfor_2605 Metfor_2608 Metfor_2609
MPLMpal_2305 Mpal_2308 Mpal_2309
MPDMCP_0512(mcrB) MCP_0515(mcrG) MCP_0516(mcrA)
MEZMtc_0904(mcrB) Mtc_0907(mcrG) Mtc_0908(mcrA)
RCIRCIX2059(mcrB) RCIX2062(mcrG) RCIX2063(mcrA)
MELUMTLP_00200 MTLP_00210 MTLP_00220 MTLP_01260(mcrB) MTLP_01290 MTLP_01300
MEAMMU439_05980(mcrA) MU439_05985(mcrG) MU439_05995(mcrB)
TARTALC_00469 TALC_00471 TALC_00472
MAXMMALV_03970 MMALV_03990 MMALV_04000
MERMMINT_15640 MMINT_15650 MMINT_15670
MEARMpt1_c01980(mcrB) Mpt1_c02000(mrtG) Mpt1_c02010(mcrA)
MEUZKRP56_02075(mcrB) KRP56_02085(mcrG) KRP56_02090(mcrA)
MARCAR505_1396 AR505_1397 AR505_1399
BARCAOA65_0406(mrtB) AOA65_0407(mcrG) AOA65_0408(mcrA)
BARBAOA66_1761(mcrA) AOA66_1769(mrtB) AOA66_1770(mcrG)
 » show all
Reference
1  [PMID:3122735]
  Authors
Bobik TA, Olson KD, Noll KM, Wolfe RS.
  Title
Evidence that the heterodisulfide of coenzyme M and 7-mercaptoheptanoylthreonine phosphate is a product of the methylreductase reaction in Methanobacterium.
  Journal
Biochem Biophys Res Commun 149:455-60 (1987)
DOI:10.1016/0006-291X(87)90389-5
Reference
2
  Authors
Ellermann, J., Hedderich, R., Boecher, R. and Thauer, R.K.
  Title
The final step in methane formation: investigations with highly purified methyl coenzyme M reductase component C from Methanobacterium thermoautotrophicum (strain Marburg).
  Journal
Eur J Biochem 184:63-68 (1988)
Reference
3  [PMID:9367957]
  Authors
Ermler U, Grabarse W, Shima S, Goubeaud M, Thauer RK
  Title
Crystal structure of methyl-coenzyme M reductase: the key enzyme of biological methane formation.
  Journal
Science 278:1457-62 (1997)
DOI:10.1126/science.278.5342.1457
  Sequence
Reference
4  [PMID:11072815]
  Authors
Signor L, Knuppe C, Hug R, Schweizer B, Pfaltz A, Jaun B.
  Title
Methane formation by reaction of a methyl thioether with a photo-excited nickel thiolate--a process mimicking methanogenesis in archaea.
  Journal
Reference
5  [PMID:20520712]
  Authors
Scheller S, Goenrich M, Boecher R, Thauer RK, Jaun B
  Title
The key nickel enzyme of methanogenesis catalyses the anaerobic oxidation of methane.
  Journal
Nature 465:606-8 (2010)
DOI:10.1038/nature09015
Other DBs
ExplorEnz - The Enzyme Database: 2.8.4.1
IUBMB Enzyme Nomenclature: 2.8.4.1
ExPASy - ENZYME nomenclature database: 2.8.4.1
UM-BBD (Biocatalysis/Biodegradation Database): 2.8.4.1
BRENDA, the Enzyme Database: 2.8.4.1
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