KEGG   ENZYME: 3.2.1.62
Entry
EC 3.2.1.62                 Enzyme                                 
Name
glycosylceramidase;
phlorizin hydrolase;
phloretin-glucosidase;
glycosyl ceramide glycosylhydrolase;
cerebrosidase;
phloridzin beta-glucosidase;
lactase-phlorizin hydrolase;
phloridzin glucosidase;
LPH (gene name);
LCT (gene name);
glycosyl-N-acylsphingosine glycohydrolase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
Sysname
beta-D-glucosyl-N-acylsphingosine glycohydrolase (configuration-retaining)
Reaction(IUBMB)
(1) a beta-D-glucosyl-N-acylsphingosine + H2O = a ceramide + beta-D-glucose [RN:R12969];
(2) a beta-D-galactosyl-N-acylsphingosine + H2O = a ceramide + beta-D-galactose [RN:R12970];
(3) a flavonoid-O-beta-D-glucoside + H2O = a flavonoid + beta-D-glucose
Reaction(KEGG)
R12969 R12970
Substrate
beta-D-glucosyl-N-acylsphingosine [CPD:C01190];
H2O [CPD:C00001];
beta-D-galactosyl-N-acylsphingosine [CPD:C02686];
flavonoid-O-beta-D-glucoside
Product
ceramide [CPD:C00195];
beta-D-glucose [CPD:C00221];
beta-D-galactose [CPD:C00962];
flavonoid [CPD:C01579]
Comment
The enzyme, found in the intestinal mucosa, hydrolyses beta-D-glucosyl and beta-D-galactosyl residues from a very broad range of substrates using a retaining mechanism. Characterized substrates include glucosyl- and galactosyl-ceramides [3], O3-, O4'- and O7-glucosylated flavonoids [6], and the 2'-O-glucosylated dihydrochalcone phlorizin [1]. The enzyme includes two glycosyl hydrolase domains, both belonging to the GH1 family. While one domain is responsible for the activity described here, the other catalyses the reaction of EC 3.2.1.108, lactase [4,5]. cf. EC 3.2.1.45, glucosylceramidase and EC 3.2.1.46, galactosylceramidase.
History
EC 3.2.1.62 created 1972, modified 1976, modified 2022
Orthology
K01229  lactase-phlorizin hydrolase
Genes
HSA3938(LCT)
PTR459633(LCT)
PPS100993974(LCT)
GGO101136936(LCT)
PON100450303(LCT)
NLE100602088(LCT)
MCC707761(LCT)
MCF102141036(LCT)
CSAB103216991(LCT)
CATY105590352(LCT)
PANU101022382(LCT)
TGE112636379(LCT)
RRO104665436(LCT)
RBB108539586(LCT)
TFN117095069(LCT)
PTEH111527488(LCT)
CJC100408648(LCT)
SBQ101044226(LCT)
CSYR103263113(LCT)
MMUR105857603(LCT)
LCAT123643641(LCT)
OGA100944107(LCT)
MMU226413(Lct)
MCAL110304188(Lct)
MPAH110322545(Lct)
RNO116569(Lct)
MCOC116098602(Lct)
MUN110555417(Lct)
CGE100766856(Lct)
MAUA101828124(Lct)
PLEU114696961(Lct)
MORG121446300 121446324 121446359
AAMP119827729 119827814 119827830
NGI103734619(Lct)
HGL101697396(Lct)
CPOC100732160(Lct)
DORD105996629(Lct)
DSP122110822(Lct)
NCAR124980608
OCU100009256(LPH) 100352497(LCT) 100353264
OPI101519618 101519868 101520351
TUP102470311(LCT)
CFA483898(LCT)
VVP112932617(LCT)
VLG121481866(LCT)
AML100484153(LCT)
UMR103667712(LCT)
UAH113263420(LCT)
UAR123782596(LCT)
ELK111143551
LLV125095933
MPUF101676191(LCT)
ORO101373825(LCT)
EJU114207344(LCT)
ZCA113920630(LCT)
MLX118012500(LCT)
FCA101092452(LCT)
PYU121030495(LCT)
PBG122479372(LCT)
PTG102949288(LCT)
PPAD109270343(LCT)
AJU106969449(LCT)
HHV120230375(LCT)
BTA514332(LCT)
BOM102279578(LCT)
BIU109568203(LCT)
BBUB102410227(LCT)
CHX102170472(LCT)
OAS101104042(LCT)
ODA120854489(LCT)
CCAD122453560(LCT)
SSC100625807(LCT) 100625897
CFR102513047(LCT)
CBAI105083393(LCT)
CDK105092305(LCT)
VPC102532468(LCT)
BACU103003658(LCT)
LVE103074579(LCT)
OOR101270599(LCT)
DLE111172712(LCT)
PCAD102988992(LCT)
PSIU116756492(LCT)
ECB100055369(LCT)
EPZ103548925(LCT)
EAI106836543(LCT)
MYB102246527(LCT)
MYD102761793(LCT)
MMYO118662079(LCT)
MLF102430551(LCT)
MNA107531139(LCT)
PKL118709739(LCT)
HAI109392715(LCT)
DRO112313063(LCT)
SHON118974532(LCT)
AJM119040600(LCT)
PDIC114495149(LCT)
PHAS123810272(LCT)
MMF118625706(LCT)
RFQ117025498(LCT)
PALE102887366(LCT)
PGIG120611943(LCT)
PVP105288339(LCT)
RAY107511200(LCT)
MJV108405363(LCT)
TOD119255787(LCT)
SARA101545875(LCT)
LAV100661599(LCT)
TMU101348169
DNM101441621(LCT)
MDO100016393(LCT)
GAS123241493(LCT)
SHR100933904(LCT)
PCW110223786(LCT)
OAA100084941(LCT)
GGA424294(LCT)
PCOC116227271(LCT)
MGP100540699(LCT)
CJO107316868(LCT)
NMEL110399364(LCT)
APLA101799746(LCT)
ACYG106039329(LCT)
AFUL116490699(LCT)
TGU100229941(LCT)
LSR110480639(LCT)
SCAN103814626(LCT)
PMOA120501755(LCT)
OTC121337155(LCT)
PRUF121356606(LCT)
GFR102041259(LCT)
FAB101814034(LCT)
PHI102100057(LCT)
PMAJ107207677(LCT)
CCAE111932317(LCT)
CCW104694217(LCT)
ETL114057697(LCT)
ZAB102069527(LCT)
FPG101921531(LCT)
FCH102058755(LCT)
CLV102086420(LCT)
EGZ104129535(LCT)
NNI104009760(LCT)
PLET104616147(LCT)
ACUN113482159(LCT)
TALA104368387(LCT)
PADL103912627(LCT)
ACHC115342953(LCT)
AAM106498455(LCT)
AROW112977064(LCT)
NPD112948593(LCT)
DNE112988323(LCT)
ASN102370133(LCT)
AMJ102576031(LCT)
CPOO109323207(LCT)
GGN109301357(LCT)
PSS102456860(LCT)
CMY102946778(LCT)
CPIC101933763(LCT)
TST117885118(LCT)
CABI116826106(LCT)
MRV120374328(LCT)
ACS103279188(lct)
PVT110071879(LCT)
SUND121925981(LCT)
PBI103054678(LCT)
PMUR107284386(LCT)
TSR106540054(LCT)
PGUT117669655(LCT)
VKO123027553(LCT)
PMUA114604625(LCT)
ZVI118092494(LCT)
GJA107115411(LCT)
STOW125426237(LCT)
XLA100127248(lct.1.L) 100127291(lct.2.L)
XTR100145766(lct.2) 101732850
NPR108791336 108791337
RTEM120944145 120944146
BBUF121008829(LCT) 121008830
BGAR122944682 122944685
DRE559107 796436(lct)
SRX107739717 107741586
SANH107688954 107704822
SGH107601359 107601360
CAUA113108294 113108295
PPRM120485547 120487230
MAMB125245778 125245780 125270691
IPU108266116
PHYP113525948(lct)
SMEO124380192
TFD113654132
AMEX103029218(lct)
EEE113577223(lct)
TRU101076621
LCO104939725(lct)
NCC104954911(lct)
CGOB115026493(lct)
ELY117248075 117251770
PLEP121949084 121949085
SLUC116044576 116044580
ECRA117953249
PFLV114563686 114564430
GAT120834464 120834490
PPUG119229302
MSAM119903852
CUD121522156 121522288 121522602
ALAT119025751 119026172
MZE101465201 101465483
ONL100703330 100703603 100703875 102080755
OAU116324944 116324945 116324946 116333422
OLA101171223(lct)
OML112155270 112155271 112155272
XMA102216752 102217013 102223171(lct)
XCO114147565 114147571 114147572
XHE116722850 116722851 116723643(lct) 116723644
PRET103461729
PFOR103152094(lct) 103153521
PLAI106942509 106945899 106945900
PMEI106908439(lct) 106920698
GAF122840135 122840136
CVG107091021(lct) 107091024
CTUL119796496 119796544
GMU124870853 124870854
NFU107389384(lct)
ALIM106522267(lct)
NWH119418815
AOCE111581081 111586173
CSEM103392433(lct)
POV109631322
SSEN122759778 122759794
HHIP117755058
HSP118120062
LCF108887080
SDU111218818
SLAL111667436
XGL120801628
HCQ109515508(lct)
MALB109966332(lct)
BSPL114847775
SASA106586119(lct)
OTW112235127
OMY110519997
OGO124043772
ONE115107792(lct)
SALP111973682(lct)
SNH120057744
ELS105005862(lct)
SFM108928209(lct)
PKI111842887(lct)
AANG118222690
LOC102691495(lct)
PSPA121323070
ARUT117407121
LCM102361997(LCT)
CMK103180388
RTP109919181(lct)
BBEL109468489 109470924
SCLV120331019
BGT106067751 106069349 106069463
HRF124113779 124151882
HRJ124256256 124256662 124256664 124266902
CRG105317659 117692405
PMAX117332095
MMER123533272 123534954
TWL119987970
 » show all
Reference
1  [PMID:5774775]
  Authors
Malathi P, Crane RK.
  Title
Phlorizin hydrolase: a beta-glucosidase of hamster intestinal brush border membrane.
  Journal
Biochim Biophys Acta 173:245-56 (1969)
DOI:10.1016/0005-2736(69)90108-4
Reference
2  [PMID:5082068]
  Authors
Lorenz-Meyer H, Blum AL, Haemmerli HP, Semenza G.
  Title
A second enzyme defect in acquired lactase deficiency: lack of small-intestinal phlorizin-hydrolase.
  Journal
Eur J Clin Invest 2:326-31 (1972)
DOI:10.1111/j.1365-2362.1972.tb00658.x
Reference
3  [PMID:4752949]
  Authors
Leese HJ, Semenza G.
  Title
On the identity between the small intestinal enzymes phlorizin hydrolase and glycosylceramidase.
  Journal
J Biol Chem 248:8170-3 (1973)
Reference
4  [PMID:9762914]
  Authors
Zecca L, Mesonero JE, Stutz A, Poiree JC, Giudicelli J, Cursio R, Gloor SM, Semenza G.
  Title
Intestinal lactase-phlorizin hydrolase (LPH): the two catalytic sites; the role of the pancreas in pro-LPH maturation.
  Journal
FEBS Lett 435:225-8 (1998)
DOI:10.1016/s0014-5793(98)01076-x
Reference
5  [PMID:11106409]
  Authors
Arribas JC, Herrero AG, Martin-Lomas M, Canada FJ, He S, Withers SG.
  Title
Differential mechanism-based labeling and unequivocal activity assignment of the two active sites of intestinal lactase/phlorizin hydrolase.
  Journal
Eur J Biochem 267:6996-7005 (2000)
DOI:10.1046/j.1432-1327.2000.01784.x
Reference
6  [PMID:12594539]
  Authors
Nemeth K, Plumb GW, Berrin JG, Juge N, Jacob R, Naim HY, Williamson G, Swallow DM, Kroon PA.
  Title
Deglycosylation by small intestinal epithelial cell beta-glucosidases is a critical step in the absorption and metabolism of dietary flavonoid glycosides in humans.
  Journal
Eur J Nutr 42:29-42 (2003)
DOI:10.1007/s00394-003-0397-3
Other DBs
ExplorEnz - The Enzyme Database: 3.2.1.62
IUBMB Enzyme Nomenclature: 3.2.1.62
ExPASy - ENZYME nomenclature database: 3.2.1.62
BRENDA, the Enzyme Database: 3.2.1.62
CAS: 9033-10-7
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