Hydrolases;
Acting on ether bonds;
Ether hydrolases
BRITE hierarchy

epoxide hydrolase

an epoxide + H2O = a glycol [RN:R02822]

R02822 > R05842 R07108 R07109 R07110 R07111
Reaction

epoxide [CPD:C00722];
H2O [CPD:C00001]

Catalyses the hydrolysis of trans-substituted epoxides, such as trans-stilbene oxide, as well as various aliphatic epoxides derived from fatty-acid metabolism [7]. It is involved in the metabolism of arachidonic epoxides (epoxyicosatrienoic acids; EETs) and linoleic acid epoxides. The EETs, which are endogenous chemical mediators, act at the vascular, renal and cardiac levels to regulate blood pressure [4,5]. The enzyme from mammals is a bifunctional enzyme: the C-terminal domain exhibits epoxide-hydrolase activity and the N-terminal domain has the activity of EC 3.1.3.76, lipid-phosphate phosphatase [1,2]. Like EC 3.3.2.9, microsomal epoxide hydrolase, it is probable that the reaction involves the formation of an hydroxyalkyl---enzyme intermediate [4,6]. The enzyme can also use leukotriene A4, the substrate of EC 3.3.2.6, leukotriene-A4 hydrolase, but it forms 5,6-dihydroxy-7,9,11,14-icosatetraenoic acid rather than leukotriene B4 as the product [9,10]. In vertebrates, five epoxide-hydrolase enzymes have been identified to date: EC 3.3.2.6 (leukotriene-A4 hydrolase), EC 3.3.2.7 (hepoxilin-epoxide hydrolase), EC 3.3.2.9 (microsomal epoxide hydrolase), EC 3.3.2.10 (soluble epoxide hydrolase) and EC 3.3.2.11 (cholesterol 5,6-oxide hydrolase) [7].

EC 3.3.2.10 created 2006 (EC 3.3.2.3 created 1978, part incorporated 2006)

1  [PMID:12574510]

2  [PMID:12574508]

3  [PMID:4584115]

4  [PMID:15822179]

5  [PMID:11090543]

6

7  [PMID:11154734]

8  [PMID:7840649]

9  [PMID:3009453]

10 [PMID:15748653]