Entry
Name
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Cysteine endopeptidases
BRITE hierarchy
Reaction(IUBMB)
All bonds known to be hydrolysed by this endopeptidase have arginine in P1 and an acidic residue in P4. P6 is often occupied by an acidic residue or by an hydroxy-amino-acid residue, the phosphorylation of which enhances cleavage
Comment
In both budding yeast and human cells, cleavage of the cohesin subunit Scc1 by separase is required for sister chromatid separation in mitosis. Budding yeast separase is also known to cleave the Rec8 subunit of a meiotic cohesin complex and the kinetochore protein Slk19. Type example of peptidase family C50.
History
EC 3.4.22.49 created 2003
Orthology
Genes
CSAB : 103238388 103238391(ESPL1)
MORG : 121444236(Espl1) 121459432
PCAD : 102974477 114486511(ESPL1)
XLA : 431925(espl1.S) 733221(espl1.L)
SANH : 107667390 107673592
CCAR : 109063482 109091441
MASI : 127446132 127449376
TFD : 113662736(espl1) 125141227
ARUT : 117400942 117967028
DSI : Dsimw501_GD13181(Dsim_GD13181)
DMN : 108151739 108163891 117189158 117189159
CEL : CELE_Y47G6A.12(sep-1) CELE_ZK430.5(ZK430.5)
CBR : CBG_14956(Cbr-sep-1)
LAK : 106162190 106162192 106173788
CSAT : 104718008 104727823 104731195
EUS : EUTSA_v10024187mg EUTSA_v10029518mg
BNA : 106373062 106375763 106398538 106440466 106440619 111214417
BOE : 106301587 106316163 106337896 106343049
MINC : 123206072 123214667
LJA : Lj0g3v0225339.1(Lj0g3v0225339.1)
AHF : 112714932 112784436 112800459
SSPL : 121753928 121758961
PSOM : 113346473 113356227
DOSA : Os02t0770700-01(Os02g0770700)
TAES : 123128537 123138403 123145658
LPER : 127302599 127305987 127317065
SITA : 101777770 101782294
PVIR : 120643306 120657316
SMO : SELMODRAFT_405667 SELMODRAFT_442839
VCN : VOLCADRAFT_96349(espA)
NCS : NCAS_0E01080(NCAS0E01080)
NDI : NDAI_0G01210(NDAI0G01210)
TPF : TPHA_0D04010(TPHA0D04010)
TBL : TBLA_0C02250(TBLA0C02250)
TDL : TDEL_0F03240(TDEL0F03240)
KAF : KAFR_0K01610(KAFR0K01610)
KNG : KNAG_0B01060(KNAG0B01060)
CAL : CAALFM_C101670CA(ESP1)
CLUS : A9F13_10g01056 A9F13_10g01067
NTE : NEUTE1DRAFT146400(NEUTE1DRAFT_146400)
SMP : SMAC_02224(putative esp1)
BFU : BCIN_10g02490(Bcesp1)
ANG : ANI_1_1636064(An07g03090)
CGI : CGB_B9070W CGB_B9080W
LBC : LACBIDRAFT_297425 LACBIDRAFT_307095 LACBIDRAFT_307111 LACBIDRAFT_317505 LACBIDRAFT_317635 LACBIDRAFT_317789
ABP : AGABI1DRAFT80074(AGABI1DRAFT_80074)
ABV : AGABI2DRAFT208386(AGABI2DRAFT_208386)
SCM : SCHCO_01122301(SCHCODRAFT_01122301)
EHI : EHI_120330(182.t00017)
SMIN : v1.2.024604.t1(symbB.v1.2.024604.t1)
» show all
Taxonomy
Reference
Authors
Waizenegger I, Gimenez-Abian JF, Wernic D, Peters JM
Title
Regulation of human separase by securin binding and autocleavage.
Journal
Sequence
Other DBs
ExPASy - ENZYME nomenclature database: 3.4.22.49
LinkDB
All DBs