KEGG ENZYME: 3.5.1.100

ENZYME: 3.5.1.100

Entry

EC 3.5.1.100                Enzyme

Name

(R)-amidase;
R-stereospecific amidase;
R-amidase

Class

Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides

Sysname

(R)-piperazine-2-carboxamide amidohydrolase

Reaction(IUBMB)

(1) (R)-piperazine-2-carboxamide + H2O = (R)-piperazine-2-carboxylate + NH3 [RN:R09647];
(2) beta-alaninamide + H2O = beta-alanine + NH3 [RN:R09648]

Reaction(KEGG)

R09647 R09648

Substrate

(R)-piperazine-2-carboxamide [CPD:C19778];
H2O [CPD:C00001];
beta-alaninamide [CPD:C19779]

Product

(R)-piperazine-2-carboxylate [CPD:C19780];
NH3 [CPD:C00014];
beta-alanine [CPD:C00099]

Comment

In addition (R)-piperidine-3-carboxamide is hydrolysed to (R)-piperidine-3-carboxylic acid and NH3, and (R)-N-tert-butylpiperazine-2-carboxamide is hydrolysed to (R)-piperazine-2-carboxylic acid and tert-butylamine with lower activity. The enzyme does not act on the other amide substrates which are hydrolysed by EC 3.5.1.4 (amidase).

History

EC 3.5.1.100 created 2009, modified 2011

Orthology

K18540

(R)-amidase

Genes

SLQ:	M495_09540

SFW:	WN53_23135

SFG:	AV650_18940

SGRI:

SGBXF1_01993(ramA_2)

PRE:	PCA10_16240

PFUW:

KF707C_40970

POJ:	PtoMrB4_44060

PLAL:

FXN65_11600

PAE:

PA3598

PAEV:

N297_3722(ramA)

PAEI:

N296_3722(ramA)

PAU:	PA14_17730

PAG:	PLES_14371

PAF:	PAM18_1387

PNC:	NCGM2_4729

PAEB:

NCGM1900_2679

PDK:	PADK2_06350

PSG:	G655_06805

PRP:	M062_19050

PAEP:

PA1S_07420

PAER:

PA1R_gp1417

PAEM:

U769_06900

PAEL:

T223_07135

PAES:

SCV20265_1459

PAEU:

BN889_03983

PAEG:

AI22_26485

PAEC:

M802_3719(ramA)

PAEO:

M801_3587(ramA)

SECH:

B18_00975

PCQ:	PcP3B5_21760(ramA_1)

PMUI:

G4G71_25745

PNT:	G5B91_08285

PAP:	PSPA7_1543

PPAA:

B7D75_07165

PPU:	PP_3846(ramA)

PPG:	PputGB1_2588

PPT:

PPS_2644

PPUH:

B479_12805

PPUD:

DW66_2904

PFV:	Psefu_1603

PMON:

X969_12555

PMOT:

X970_12200

PPJ:	RK21_02127

POR:	APT59_07350

PMOL:

CLJ08_04110

PALD:

LU682_010930

PIX:	RIN61_25620

PMOS:

O165_014445

PFL:

PFL_4898

PPRC:

PFLCHA0_c48790(ramA)

PPRO:

PPC_4907

PFO:	Pfl01_4557

PMAN:

OU5_4712

PTV:	AA957_08470

PAZO:

AYR47_12840

PTOL:

I7845_05540

PFX:	A7318_04835

PEN:

PSEEN3582

PLUL:

FOB45_04765

PPUU:

PputUW4_01010

PDR:	H681_06995

PKC:	PKB_3494(ramA)

PCH:	EY04_25420

PCZ:	PCL1606_12510

PCHP:

C4K32_5101

PFZ:	AV641_09915

PALK:

PSAKL28_32040

PSW:	LK03_17965

PSEM:

TO66_25495

PFK:	PFAS1_05720

PANR:

A7J50_1111

PSET:

THL1_1666

PSIL:

PMA3_19055

PYM:	AK972_0917 AK972_2857

PKE:	DLD99_23115

PUM:	HGP31_25555

PPSH:

G5J76_17110

PGY:	AWU82_26835

PMAO:

PMYSY11_2422(ramA)

PATA:

JWU58_06245

PDW:

BV82_3432

PZE:	HU754_024020

PMOE:

HV782_023400

PVW:	HU752_027095

PTZ:	HU718_024630

PXN:	HU772_016120

PHV:	HU739_023445

PAZE:

KSS91_05920

PALV:

KSS97_24420

PTK:	EXN22_12150

PTW:	TUM18999_48310

PIE:	HU724_022700

PWY:	HU734_019110

PGF:	J0G10_25020

PCAV:

D3880_16645

PIZ:	LAB08_R09960

PPEG:

KUA23_05735

PFIT:

KJY40_24405

PASI:

LG197_18770

PNB:	NK667_01415

PSOA:

PSm6_34730

PKJ:	Q1W70_00975

PHOM:

KJF94_20945

PTRL:

OU419_07405

PSHS:

JJN09_13355

PSNC:

C0058_26465

PBEJ:

PSH84_00910

PBAM:

NUH87_22990

PSDT:

NH234_24060

PCAA:

V6L78_29060

PBUB:

V6B39_16830

PSML:

V6P94_17315

PSMZ:

V6L79_16890

PSWO:

SD196_06705 SD196_09550 SD196_17125

PMOR:

PMm318_A22400 PMm318_A49870

PSSX:

DCC84_27840

PAGB:

AWM79_10040

PSNS:

CI807_12505

PSRU:

CCX46_23965

PBAB:

AAEO81_07130 AAEO81_19665

PKH:	JLK41_23925

PDEN:

F1C79_00305

CPS:

CPS_2030

CSA:

Csal_0061

CCAG:

SR908_07685

HHU:	AR456_00330

HHAO:

QWG60_00240

HADN:

NKF27_00330

HCK:	MRB56_00315

KUY:	FY550_15230

TAU:

Tola_1341

IGN:	MMG00_02635

SALN:

SALB1_0801

PSE:

NH8B_1826

AMAH:

DLM_2428

AQS:	DKK66_14490

BVI:	Bcep1808_1625

BVE:	AK36_2221(ramA)

BUR:	Bcep18194_A4513

BCM:	Bcenmc03_1348 Bcenmc03_3529 Bcenmc03_4306

BCH:	Bcen2424_3994

BCJ:	BCAL1380(ramA)

BCEN:

DM39_1273(ramA)

BCEO:

I35_1275(ramA)

BAM:

Bamb_1638

BMJ:	BMULJ_03495

BMU:

Bmul_5017

BMUL:

NP80_1792(ramA)

BCT:

GEM_2060

BCED:

DM42_329(ramA)

BCEP:

APZ15_10660

BDL:	AK34_1396(ramA)

BPYR:

ABD05_12660

BCON:

NL30_08850

BTEI:

WS51_07825

BSEM:

WJ12_06730

BPSL:

WS57_25140

BMEC:

WJ16_06595

BSTL:

BBJ41_05260

BANN:

JFN94_06975

BARI:

NLX30_07390

BAEN:

L3V59_07455

BSER:

R0290_08600

BGL:	bglu_2g15230

BGU:	KS03_4414(ramA)

BGD:	bgla_2g09710

BGO:	BM43_4600(ramA)

BUK:

MYA_1509

BGP:	BGL_2c08780

BPLA:

bpln_2g09690

BURM:

GJG85_16840

BURF:

Bsp3421_001338 Bsp3421_006762

BUZ:	AYM40_06155 AYM40_21415

BFN:

OI25_201

BCAI:

K788_0003819

PARB:

CJU94_17435

PHS:	C2L64_06765

PTER:

C2L65_06505

PCAF:

DSC91_006605

PMEG:

FNZ07_25755

PTRO:

G5S35_20385

PACP:

FAZ97_23945

PACS:

FAZ98_19315

PBRY:

NDK50_06505

PSAA:

QEN71_06970

PPAI:

E1956_25350 E1956_45145

PLAD:

PPGU16_13040

PAZZ:

PBP221_14120

ASW:	CVS48_09000

ACHR:

C2U31_18595

DEL:	DelCs14_5435

DTS:	BI380_19195

DHK:	BO996_05515

DLA:	I6G47_19105

RID:	RIdsm_00233(ramA_1)

GPN:	Pan110_34850(ramA_3)

MRI:	Mal4_43390(ramA_5)

SACI:

Sinac_2712

AGV:	OJF2_67910(ramA_4)

SPIB:

G8759_26060

CSPG:

LS684_22545

NCM:	QNK12_03740

SFOR:

QNH23_01270

PUK:	PU629_13185

DCA:	Desca_1581

ADG:

Adeg_1492

MSG:	MSMEI_6550

MSB:	LJ00_33265

MSN:	LI99_33270

MSH:	LI98_33275

SCYN:

N8I84_01835

CWO:

Cwoe_0991

MEUZ:

KRP56_07785

CMA:

Cmaq_0501

VDI:

Vdis_2026

VMO:

VMUT_0433

» show all

Reference

1 [PMID:15066183]

Authors

Komeda H, Harada H, Washika S, Sakamoto T, Ueda M, Asano Y

Title

A novel R-stereoselective amidase from Pseudomonas sp. MCI3434 acting on piperazine-2-tert-butylcarboxamide.

Journal

Eur J Biochem 271:1580-90 (2004)
DOI:10.1111/j.1432-1033.2004.04069.x

Sequence

[ag:BAD15093]

Other DBs

ExplorEnz - The Enzyme Database:

3.5.1.100

IUBMB Enzyme Nomenclature:

3.5.1.100

ExPASy - ENZYME nomenclature database:

3.5.1.100

BRENDA, the Enzyme Database:

3.5.1.100

LinkDB

DBGET integrated database retrieval system