KEGG   ENZYME: 3.5.1.114
Entry
EC 3.5.1.114                Enzyme                                 
Name
N-acyl-aromatic-L-amino acid amidohydrolase;
aminoacylase 3;
aminoacylase III;
ACY3 (gene name)
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
Sysname
N-acyl-aromatic-L-amino acid amidohydrolase (carboxylate-forming)
Reaction(IUBMB)
(1) an N-acyl-aromatic-L-amino acid + H2O = an aromatic-L-amino acid + a carboxylate [RN:R10621];
(2) an N-acetyl-L-cysteine-S-conjugate + H2O = an L-cysteine-S-conjugate + acetate [RN:R10553]
Reaction(KEGG)
R10553 R10621
Substrate
N-acyl-aromatic-L-amino acid [CPD:C20735];
H2O [CPD:C00001];
N-acetyl-L-cysteine S-conjugate [CPD:C05727]
Product
aromatic-L-amino acid [CPD:C01021];
carboxylate [CPD:C00060];
L-cysteine S-conjugate [CPD:C05726];
acetate [CPD:C00033]
Comment
This enzyme is found in animals and is involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate). It preferentially deacetylates Nalpha-acetylated aromatic amino acids and mercapturic acids (S-conjugates of N-acetyl-L-cysteine) that are usually not deacetylated by EC 3.5.1.14, N-acyl-aliphatic-L-amino acid amidohydrolase. The enzyme is significantly activated by Co2+ and Ni2+ [3]. Some bacterial aminoacylases demonstrate substrate specificity for both EC 3.5.1.14 and EC 3.5.1.114. cf. EC 3.5.1.14, N-acyl-aliphatic-L-amino acid amidohydrolase and EC 3.5.1.15, aspartoacylase.
History
EC 3.5.1.114 created 2013
Orthology
K18458  N-acyl-aromatic-L-amino acid amidohydrolase
Genes
HSA91703(ACY3)
PTR451373(ACY3)
PPS100992485(ACY3)
GGO101144518(ACY3)
PON100454588(ACY3)
NLE100581974(ACY3)
MCC711083(ACY3)
MCF102123426(ACY3)
CATY105577339(ACY3)
TGE112606840(ACY3)
RRO104664723(ACY3)
RBB108518683(ACY3)
TFN117081003(ACY3)
PTEH111522484(ACY3)
CJC103796426(ACY3)
SBQ101028900(ACY3)
CSYR103249221(ACY3)
MMUR105867425(ACY3)
OGA100965331(ACY3)
MMU71670(Acy3)
MCAL110285586(Acy3)
MPAH110332220(Acy3)
RNO293653(Acy3)
MCOC116100997(Acy3)
MUN110547162(Acy3)
CGE100763612(Acy3)
PLEU114694703(Acy3)
NGI103725050
HGL101725542(Acy3)
CPOC100735640(Acy3)
CCAN109692320(Acy3)
DORD105989260(Acy3)
DSP122104969(Acy3)
NCAR124958758
OPI101528679(ACY3)
TUP102503277(ACY3)
CFA100683126(ACY3)
VLG121471657(ACY3)
ORO101362499(ACY3)
EJU114220247(ACY3)
FCA101083207(ACY3)
PYU121023739(ACY3)
PBG122484384(ACY3)
PTG102970090(ACY3)
PPAD109246993(ACY3)
BACU103009750(ACY3)
LVE103079001(ACY3)
OOR101270966(ACY3)
DLE111184037(ACY3)
PCAD102979768
ECB102149799(ACY3)
EPZ103544301
MYB102254475(ACY3) 102254790
MYD102764000(ACY3)
MMYO118665208(ACY3)
MLF102425715(ACY3)
MNA107537402(ACY3)
PKL118706856(ACY3)
HAI109384127(ACY3)
DRO112317043(ACY3)
SHON118998407(ACY3)
AJM119041818(ACY3)
PDIC114498536(ACY3)
PHAS123829379(ACY3)
MMF118629081(ACY3)
RFQ117029799(ACY3)
PALE102895489(ACY3)
PGIG120584744(ACY3)
PVP105306573(ACY3)
RAY107503948(ACY3)
LAV100668205(ACY3)
DNM101411811(ACY3)
MDO100013740(ACY3)
GAS123251613(ACY3)
SHR100933605(ACY3)
PCW110195585(ACY3)
OAA100680665(ACY3)
ASN102373930(ACY3)
AMJ102567904(ACY3)
CPOO109324624(ACY3)
PSS102461093(ACY3)
CMY102932193(ACY3)
CPIC101949865(ACY3)
TST117876660(ACY3)
CABI116833433(ACY3)
MRV120393383(ACY3)
ACS100558126(acy3)
PVT110085674(ACY3)
SUND121919516(ACY3)
PBI103058598(ACY3)
PMUR107293628(ACY3)
TSR106538901(ACY3)
PGUT117675332(ACY3)
VKO123027525(ACY3)
PMUA114602141(ACY3)
ZVI118090290(ACY3)
STOW125426187(ACY3)
XLA779340(acy3.L)
XTR595015(acy3)
NPR108791080 108797385(ACY3)
RTEM120909172(ACY3) 120926705
BBUF120995479
BGAR122931978
DRE436619(acy3.2) 791456(acy3.1)
SRX107714473 107719252 107746091 107746117 107757064
SANH107657988 107676738 107703715 107703856
SGH107570819 107570825 107571911 107572870 107575636
CCAR109045324 109065132 109065133 109112463(acy3)
CAUA113044079 113044751 113062648 113084557 113084558
PPRM120480155 120489148
MAMB125264089 125271860 125271861(acy3.2)
IPU108260612 108260764
PHYP113524540 113545754
SMEO124381101 124381703
TFD113641413 113650046
AMEX103024465 103026039
EEE113583873 113585886
TRU101061157 101063284
TNGGSTEN00011466G001 GSTEN00033497G001
LCO104923975 104926981
NCC104941420 104952637
CGOB115023841
ELY117246420 117254598
PLEP121942252 121961819
SLUC116034328 116049044
ECRA117934689 117955546
PFLV114545402 114568528
GAT120822705 120825842
PPUG119218282 119222729
MSAM119898960 119913073
CUD121505075 121508583
ALAT119020527 119027849
MZE101464677 101479803
ONL100691766 100698506
OAU116310417 116330058
OLA101159859
OML112156043 112157362(acy3.2)
XMA102227873 102230655
XCO114144463 114157942
XHE116720057 116733088
PRET103466257 103480428
PFOR103137475 103137811 103137813 103148456
PLAI106948229 106948230 106949604 106958436
PMEI106919317 106931340
GAF122819991 122829907(acy3.2)
CVG107091125 107100543
CTUL119796250 119799302
GMU124868723 124880988
NFU107375298 107377364
KMR108232961 108248517
ALIM106517035 106525346
NWH119408058 119409283
AOCE111567147 111583230
CSEM103383512
POV109637766 109642556
SSEN122767038 122770931
HHIP117751997 117766206
LCF108896521 108897067
SDU111233002 111236611
SLAL111670415 111673487
XGL120797703 120800179
HCQ109511486 109520289
BPEC110159468 110165380
MALB109961401 109974273
BSPL114857624 114865726
SASA100194544(acy3) 106577455 106609950(ACY3)
OTW112231784 112261060 112261397
OMY110497470 110531924 110534382
OGO124001891 124008767 124012125
ONE115137305 115138221 115145856
SALP111958486 111962015 112072352
SNH120030135 120034913 120052833
ELS105008212 105029486
SFM108929993 108931130
PKI111834701 111838492
AANG118227937 118231297
LOC102690242
PSPA121302115 121320145
LCM102356030 102356309
EPA110237552
PDAM113674876
SPIS111333775
SMINv1.2.033226.t1(symbB.v1.2.033226.t1) v1.2.039538.t1(symbB.v1.2.039538.t1)
AAFAURANDRAFT_21222
SPARSPRG_00693
EHXEMIHUDRAFT_252071 EMIHUDRAFT_44278 EMIHUDRAFT_44328 EMIHUDRAFT_75471
GTTGUITHDRAFT_118300
VAGN646_4536
VHRAL538_18035
VNIVIBNI_B1226
VSHBSZ05_19600
HJABST95_01785
FRTF7308_1921
FRFLO80_06370
FMIF0R74_08170
GSNYC6258_00922
NISNIS_0887
LETO77CONTIG1_03089
LBOLBWT_610
THEUHPC62_07415
MARMAE_01890
MIQB5D77_07280
MVZmyaer102_17080 myaer102_36990
ARPNIES39_O04520
LFSHFV01_10205
NOECLI64_04765
ANBANA_C13654
CALTCal6303_5356
CALHIJ00_18430
CCURIAR63_00310
TOQHCG51_04425
FSUFisuc_0510
FSCFSU_0931
 » show all
Reference
1  [PMID:14656720]
  Authors
Pushkin A, Carpenito G, Abuladze N, Newman D, Tsuprun V, Ryazantsev S, Motemoturu S, Sassani P, Solovieva N, Dukkipati R, Kurtz I
  Title
Structural characterization, tissue distribution, and functional expression of murine aminoacylase III.
  Journal
Am J Physiol Cell Physiol 286:C848-56 (2004)
DOI:10.1152/ajpcell.00192.2003
  Sequence
[mmu:71670]
Reference
2  [PMID:17012540]
  Authors
Newman D, Abuladze N, Scholz K, Dekant W, Tsuprun V, Ryazantsev S, Bondar G, Sassani P, Kurtz I, Pushkin A
  Title
Specificity of aminoacylase III-mediated deacetylation of mercapturic acids.
  Journal
Drug Metab Dispos 35:43-50 (2007)
DOI:10.1124/dmd.106.012062
  Sequence
[mmu:71670]
Reference
3  [PMID:19362172]
  Authors
Tsirulnikov K, Abuladze N, Newman D, Ryazantsev S, Wolak T, Magilnick N, Koag MC, Kurtz I, Pushkin A
  Title
Mouse aminoacylase 3: a metalloenzyme activated by cobalt and nickel.
  Journal
Biochim Biophys Acta 1794:1049-57 (2009)
DOI:10.1016/j.bbapap.2009.03.022
Reference
4  [PMID:20921362]
  Authors
Hsieh JM, Tsirulnikov K, Sawaya MR, Magilnick N, Abuladze N, Kurtz I, Abramson J, Pushkin A
  Title
Structures of aminoacylase 3 in complex with acetylated substrates.
  Journal
Proc Natl Acad Sci U S A 107:17962-7 (2010)
DOI:10.1073/pnas.1006687107
  Sequence
[mmu:71670]
Reference
5  [PMID:22819785]
  Authors
Tsirulnikov K, Abuladze N, Bragin A, Faull K, Cascio D, Damoiseaux R, Schibler MJ, Pushkin A
  Title
Inhibition of aminoacylase 3 protects rat brain cortex neuronal cells from the toxicity of 4-hydroxy-2-nonenal mercapturate and 4-hydroxy-2-nonenal.
  Journal
Toxicol Appl Pharmacol 263:303-14 (2012)
DOI:10.1016/j.taap.2012.07.002
  Sequence
[rno:293653]
Other DBs
ExplorEnz - The Enzyme Database: 3.5.1.114
IUBMB Enzyme Nomenclature: 3.5.1.114
ExPASy - ENZYME nomenclature database: 3.5.1.114
BRENDA, the Enzyme Database: 3.5.1.114
LinkDB

DBGET integrated database retrieval system