Lyases;
Carbon-carbon lyases;
Oxo-acid-lyases
BRITE hierarchy

chorismate pyruvate-lyase (amino-accepting; anthranilate-forming)

chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate (overall reaction) [RN:R00986];
(1a) L-glutamine + H2O = L-glutamate + NH3;
(1b) chorismate + NH3 = (2S)-2-amino-4-deoxychorismate + H2O;
(1c) (2S)-2-amino-4-deoxychorismate = anthranilate + pyruvate

R00986;
(other) R00985
Reaction

chorismate [CPD:C00251];
L-glutamine [CPD:C00064];
H2O [CPD:C00001];
NH3 [CPD:C00014];
(2S)-2-amino-4-deoxychorismate [CPD:C18054]

The enzyme, found in plants, fungi and bacteria is composed of two parts, a glutaminase subunit and a lyase subunit. The glutaminase produces ammonia that is channeled to the lyase subunit. In the absence of the glutaminase, the lyase can convert ammonia and chorismate into anthranilate. In some organisms, this enzyme is part of a multifunctional protein, together with one or more other components of the system for the biosynthesis of tryptophan [EC 2.4.2.18 (anthranilate phosphoribosyltransferase), EC 4.1.1.48 (indole-3-glycerol-phosphate synthase), EC 4.2.1.20 (tryptophan synthase) and EC 5.3.1.24 (phosphoribosylanthranilate isomerase)].

EC 4.1.3.27 created 1972, modified 2022