KEGG ENZYME: 4.2.1.107

ENZYME: 4.2.1.107

Entry

EC 4.2.1.107                Enzyme

Name

3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA hydratase;
46 kDa hydratase 2;
(24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA hydro-lyase

Class

Lyases;
Carbon-oxygen lyases;
Hydro-lyases

Sysname

(24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA hydro-lyase [(24E)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA-forming]

Reaction(IUBMB)

(24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA = (24E)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA + H2O [RN:R04813]

Reaction(KEGG)

R04813;
(other) R04809

Substrate

(24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA [CPD:C15614]

Product

(24E)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA [CPD:C05460];
H2O [CPD:C00001]

Comment

This enzyme forms part of the rat peroxisomal multifunctional enzyme perMFE-2, which also exhibits a dehydrogenase activity. The enzyme is involved in the beta-oxidation of the cholesterol side chain in the cholic-acid-biosynthesis pathway.

History

EC 4.2.1.107 created 2005

Pathway

ec00120

Primary bile acid biosynthesis

ec01100

Metabolic pathways

Orthology

K12405

(3R)-3-hydroxyacyl-CoA dehydrogenase / 3a,7a,12a-trihydroxy-5b-cholest-24-enoyl-CoA hydratase / enoyl-CoA hydratase 2

Genes

HSA:	3295(HSD17B4)

PTR:	462019(HSD17B4)

PPS:	100981028(HSD17B4)

GGO:	101129538(HSD17B4)

PON:	100448539(HSD17B4)

NLE:	100590035(HSD17B4)

HMH:	116470899(HSD17B4)

MCC:	698369(HSD17B4)

MCF:	102123675(HSD17B4)

MTHB:

126957048

MNI:	105500031(HSD17B4)

CSAB:

103244395(HSD17B4)

CATY:

105590649(HSD17B4)

PANU:

101004327(HSD17B4)

TGE:	112625827(HSD17B4)

MLEU:

105534163(HSD17B4)

RRO:	104654581(HSD17B4)

RBB:	108529825(HSD17B4)

TFN:	117065971(HSD17B4)

PTEH:

111530208(HSD17B4)

CANG:

105527387(HSD17B4)

CJC:	100390100(HSD17B4)

SBQ:	101036776(HSD17B4)

CIMI:

108306790(HSD17B4)

CSYR:

103262046

MMUR:

105872240

LCAT:

123648404(HSD17B4)

PCOQ:

105822547(HSD17B4)

OGA:	100966879(HSD17B4)

MMU:	15488(Hsd17b4)

MCAL:

110284447(Hsd17b4)

MPAH:

110332881(Hsd17b4)

RNO:	79244(Hsd17b4)

MCOC:

116087150(Hsd17b4)

ANU:	117719952(Hsd17b4)

CGE:	103161104(Hsd17b4)

MAUA:

101827399(Hsd17b4)

PROB:

127234029(Hsd17b4)

PLEU:

114694768(Hsd17b4)

MORG:

121441957 121453615(Hsd17b4)

MFOT:

126505968

AAMP:

119815878(Hsd17b4)

NGI:	103745113(Hsd17b4)

HGL:	101713520(Hsd17b4)

CPOC:

100379226(Hsd17b4)

CCAN:

109674145

DORD:

105985471(Hsd17b4)

DSP:	122101973(Hsd17b4)

PLOP:

125341824(Hsd17b4)

NCAR:

124986751

OCU:

100346781

OPI:	101530287(HSD17B4)

TUP:	102468828(HSD17B4)

GVR:	103605910(HSD17B4)

CFA:	474630(HSD17B4)

CLUD:

112659672(HSD17B4)

VVP:	112932403(HSD17B4)

VLG:	121494637(HSD17B4)

NPO:	129494700(HSD17B4)

AML:	100471749(HSD17B4)

UMR:	103670554(HSD17B4)

UAH:	113261629(HSD17B4)

UAR:	123790430(HSD17B4)

ELK:

111158891

LLV:

125100000

MPUF:

101683185(HSD17B4)

NVS:	122916269(HSD17B4)

ORO:	101385731(HSD17B4)

EJU:	114209435(HSD17B4)

ZCA:	113928790(HSD17B4)

MLX:	118016560(HSD17B4)

NSU:	110590913(HSD17B4)

LWW:	102736737(HSD17B4)

FCA:	101091840(HSD17B4)

PYU:	121010581(HSD17B4)

PCOO:

112861741(HSD17B4)

PBG:	122486641(HSD17B4)

LRUF:

124515159

PTG:	102968873(HSD17B4)

PPAD:

109278892(HSD17B4)

PUC:

125934835

AJU:

106975089

HHV:	120240242(HSD17B4)

BTA:	493643(HSD17B4)

BOM:	102268517(HSD17B4)

BBUB:

102412917(HSD17B4)

BBIS:

104993366(HSD17B4)

CHX:	102172142(HSD17B4)

OAS:	101114519(HSD17B4)

BTAX:

128050558(HSD17B4)

ODA:	120864671(HSD17B4)

CCAD:

122440074(HSD17B4)

SSC:	397574(HSD17B4)

CFR:	102508317(HSD17B4)

CBAI:

105082053(HSD17B4)

CDK:	105104293(HSD17B4)

VPC:	102537534(HSD17B4)

LVE:	103084787(HSD17B4)

OOR:	101282963(HSD17B4)

DLE:	111183342(HSD17B4)

PCAD:

102996127(HSD17B4)

PSIU:

116751686(HSD17B4)

NASI:

112398657(HSD17B4)

ECB:	100034046(HSD17B4)

EPZ:	103548315(HSD17B4)

EAI:	106833039(HSD17B4)

MYB:	102240475(HSD17B4)

MYD:	102767159(HSD17B4)

MMYO:

118656167(HSD17B4)

MLF:	102425700(HSD17B4)

MNA:	107530713(HSD17B4)

PKL:	118703106(HSD17B4)

HAI:	109374101(HSD17B4)

DRO:	112315031(HSD17B4)

SHON:

118975196(HSD17B4)

AJM:	119051192(HSD17B4)

PDIC:

114494306(HSD17B4)

PHAS:

123818113(HSD17B4)

MMF:	118642891(HSD17B4)

RFQ:	117024890(HSD17B4)

PALE:

102887000(HSD17B4)

PGIG:

120590862(HSD17B4)

PVP:	105290860(HSD17B4)

RAY:	107511154(HSD17B4)

TOD:	119243559(HSD17B4)

SARA:

101542842(HSD17B4)

LAV:	100659299(HSD17B4)

TMU:

101356123

ETF:	101641151(HSD17B4) 101644420

DNM:	101423884(HSD17B4)

MDO:	100028098(HSD17B4)

GAS:	123235791(HSD17B4)

SHR:	100932771(HSD17B4)

PCW:	110202743(HSD17B4)

OAA:	100081754(HSD17B4)

GGA:	395785(HSD17B4)

PCOC:

116234067(HSD17B4)

CJO:	107306670(HSD17B4)

TPAI:

128089355(HSD17B4)

NMEL:

110389447(HSD17B4)

APLA:

101791118(HSD17B4)

ACYG:

106048362(HSD17B4)

AFUL:

116500754(HSD17B4)

TGU:	100218226(HSD17B4)

LSR:	110479177(HSD17B4)

SCAN:

103821465(HSD17B4)

PMOA:

120512524(HSD17B4)

OTC:	121332706(HSD17B4)

PRUF:

121362562(HSD17B4)

GFR:	102036502(HSD17B4)

FAB:	101807233(HSD17B4)

PHI:	102109547(HSD17B4)

PMAJ:

107216347(HSD17B4)

CCAE:

111941198(HSD17B4)

CCW:	104690497(HSD17B4)

CBRC:

103611629(HSD17B4)

ETL:	114056305(HSD17B4)

ZAB:	102062238(HSD17B4)

ACHL:

103806159(HSD17B4)

SVG:	106851983(HSD17B4)

FPG:	101917400(HSD17B4)

FCH:	102058406(HSD17B4)

CLV:	102095771(HSD17B4)

EGZ:	104122491(HSD17B4)

NNI:	104014571(HSD17B4)

PLET:

104618021(HSD17B4)

ACUN:

113489653(HSD17B4)

TALA:

104363287(HSD17B4)

PADL:

103912347(HSD17B4)

AFOR:

103897966(HSD17B4)

ACHC:

115336530(HSD17B4)

HALD:

104320961(HSD17B4)

HLE:	104828588(HSD17B4)

AGEN:

126036470

GCL:

127027587

CSTI:

104557391(HSD17B4)

CMAC:

104480460(HSD17B4)

MUI:	104541285(HSD17B4)

BREG:

104629512(HSD17B4)

FGA:	104069667(HSD17B4)

LDI:	104348760(HSD17B4)

OHA:	104339186(HSD17B4)

NNT:	104407165(HSD17B4)

SHAB:

115619609(HSD17B4)

DPUB:

104304758(HSD17B4)

PGUU:

104458725(HSD17B4)

CPEA:

104392915(HSD17B4)

AVIT:

104272741(HSD17B4)

CVF:	104294213(HSD17B4)

CUCA:

104061043(HSD17B4)

BRHI:

104495883(HSD17B4)

AROW:

112978041(HSD17B4)

NPD:	112958912(HSD17B4)

TGT:	104571515(HSD17B4)

DNE:

112992405

SCAM:

104150534(HSD17B4)

ASN:	102371766(HSD17B4)

AMJ:	102571005(HSD17B4)

CPOO:

109323436(HSD17B4)

GGN:	109297318(HSD17B4)

PSS:	102451304(HSD17B4)

CMY:	102933522(HSD17B4)

CPIC:

101945395(HSD17B4)

TST:	117879364(HSD17B4)

CABI:

116836212(HSD17B4)

MRV:	120408024(HSD17B4)

ACS:	100558484(hsd17b4)

PVT:	110084344(HSD17B4)

SUND:

121921111(HSD17B4)

PBI:	103063870(HSD17B4)

PMUR:

107294349(HSD17B4)

CTIG:

120316078(HSD17B4)

TSR:	106543382(HSD17B4)

PGUT:

117674677(HSD17B4)

PTEX:

113449307(HSD17B4)

NSS:	113415957(HSD17B4)

VKO:	123031365(HSD17B4)

PMUA:

114606393(HSD17B4)

ZVI:	118076708(HSD17B4)

HCG:	128345823(HSD17B4)

GJA:	107116225(HSD17B4)

STOW:

125436558(HSD17B4)

XLA:	444492(hsd17b4.L)

XTR:	613082(hsd17b4)

NPR:	108787696(HSD17B4)

RTEM:

120931986(HSD17B4)

BBUF:

120992600(HSD17B4)

BGAR:

122924617(HSD17B4)

DRE:	393105(hsd17b4)

SANH:

107703629(hsd17b4)

SGH:	107571774(hsd17b4)

CAUA:

113094195 113106903

CGIB:

127963370

PTET:

122350586(hsd17b4)

LROH:

127169979(hsd17b4)

PPRM:

120475505(hsd17b4)

MAMB:

125279588(hsd17b4)

CIDE:

127517328

MASI:

127414554 127423847

IPU:

108265438

PHYP:

113529268(hsd17b4)

SMEO:

124399595(hsd17b4)

TFD:	113656214(hsd17b4)

AMEX:

103046836(hsd17b4)

CMAO:

118813245(hsd17b4)

EEE:	113571049(hsd17b4)

CHAR:

105907549(hsd17b4)

TRU:	101062498(hsd17b4)

TNG:	GSTEN00030945G001

LCO:	104920820(hsd17b4)

NCC:	104966339(hsd17b4)

CGOB:

115013548(hsd17b4)

ELY:	117252434(hsd17b4)

EFO:	125890843(hsd17b4)

PLEP:

121944362(hsd17b4)

SLUC:

116054060(hsd17b4)

ECRA:

117944965(hsd17b4)

ESP:	116689590(hsd17b4)

PFLV:

114555481(hsd17b4)

GAT:	120830783(hsd17b4)

PPUG:

119225552(hsd17b4)

MSAM:

119891683(hsd17b4)

SCHU:

122876928(hsd17b4)

CUD:	121509730(hsd17b4)

ALAT:

119019193(hsd17b4)

MZE:	101482787(hsd17b4)

ONL:	100706762(hsd17b4)

OAU:	116310500(hsd17b4)

OLA:	101159921(hsd17b4)

OML:	112148954(hsd17b4)

XMA:	102223774(hsd17b4)

XCO:	114154573(hsd17b4)

XHE:	116730397(hsd17b4)

PRET:

103470095(hsd17b4)

PFOR:

103141760(hsd17b4)

PLAI:

106944940(hsd17b4)

PMEI:

106923064(hsd17b4)

GAF:	122827419(hsd17b4)

CVG:	107086311(hsd17b4)

CTUL:

119798579(hsd17b4)

GMU:	124877861(hsd17b4)

NFU:	107394149(hsd17b4)

KMR:	108233704(hsd17b4)

ALIM:

106513677(hsd17b4)

NWH:	119413904(hsd17b4)

AOCE:

111564685(hsd17b4)

MCEP:

125012708(hsd17b4)

POV:	109637138(hsd17b4)

SSEN:

122769476(hsd17b4)

HHIP:

117768550(hsd17b4)

HSP:	118114529(hsd17b4)

LCF:

108882142

SDU:	111230866(hsd17b4)

SLAL:

111645149(hsd17b4)

XGL:	120805559(hsd17b4)

HCQ:	109526033(hsd17b4)

SSCV:

125993727

BPEC:

110167024(hsd17b4)

MALB:

109956607(hsd17b4)

BSPL:

114861822(hsd17b4)

SASA:

106585849(hsd17b4)

STRU:

115164957(hsd17b4)

OTW:	112247069(hsd17b4)

OMY:	110525264(hsd17b4)

OGO:	124034633(hsd17b4)

ONE:	115111275(hsd17b4)

SALP:

111974039(hsd17b4)

SNH:	120045172(hsd17b4)

CCLU:

121584411(hsd17b4)

ELS:	105014609(hsd17b4)

SFM:	108938133(hsd17b4)

PKI:	111856620(hsd17b4)

AANG:

118206736(hsd17b4)

LOC:	102697531(hsd17b4)

PSPA:

121302596(hsd17b4) 121316706

ARUT:

117427495 117973220

PSEX:

120532694(hsd17b4)

LCM:	102356548(HSD17B4)

CMK:	103181119(hsd17b4)

CPLA:

122560645(hsd17b4)

BFO:

118409354

BBEL:

109480804

CIN:

100187421

SCLV:

120338122

SPU:	115918401 581580

AJC:

117122621

DME:	Dmel_CG3415(Mfe2)

DER:

6550509

DSE:

116802232

DSI:	Dsimw501_GD29376(Dsim_GD29376)

DYA:	Dyak_GE17252

DAN:

6501789

DSR:

110184585

DPO:

4815128

DPE:

6599365

DMN:

108161558

DWI:

6648554

DGR:

6565453

DMO:	Dmoj_GI11209

DAZ:

108619615

DNV:

108656784

DHE:

111598829

DVI:

6636673

CCAT:

101461807

BOD:

106614681

RZE:	108376746 108381954

MDE:

101897498

SCAC:

106092762

LCQ:

111686897

LSQ:

119599856

HIS:

119648611

AGA:	AgaP_AGAP008667

ACOZ:

120955687

AARA:

120901803

AMER:

121597830

ASTE:

118512713

AALI:

118461846

AAG:

5568633

AALB:

109428196

CQU:	CpipJ_CPIJ001576

CPII:

120414661

CNS:

116337519

BCOO:

119084844

AME:

409986

ACER:

107999187

ALAB:

122718722

BIM:

100745646

BBIF:

117204816

BVK:

117231189

BVAN:

117163388

BTER:

100648641

BPYO:

122570889

CCAL:

108626230

OBB:

114872784

MGEN:

117224638

NMEA:

116425205

SOC:

105192979

MPHA:

105839781

AEC:

105149946

ACEP:

105621202

HST:

105188161

DQU:

106744052

CFO:

105248091

FEX:

115245059

LHU:

105679879

PGC:

109854791

OBO:

105280041

PCF:

106793023

PFUC:

122520011

VPS:

122631462

NVI:

100123018

CSOL:

105368036

TPRE:

106659616

LHT:

122501071

LBD:

127277949

MDL:

103579980

CGLO:

123268667

FAS:

105272013

DAM:

107040917

AGIF:

122847409

CINS:

118067462

CCIN:

107263397

DSM:

124411878

NPT:

124218728

NFB:

124183016

NLO:

107228102

NVG:

124305349

DPA:

109536257

SOY:

115888404

AGRG:

126743269

ATD:

109606046

CSET:

123306476

AGB:

108915760

LDC:

111505860

NVL:

108562871

APLN:

108736403

PPYR:

116158989 116159225 116159796 116159821 116176974 116179454

OTU:

111423793

BMOR:

101745499

BMAN:

114242087

MSEX:

115443231

NIQ:

126775080

PMAC:

106711197

PPOT:

106102091

PXU:

106114302

PRAP:

110991438

PBX:

123713006

PNAP:

125062929

ZCE:

119831635

CCRC:

123699116

HAW:

110370579

HZE:

124638194

TNL:

113504311

SLIU:

111349601

OFU:

114351201

PXY:

105391367

CFEL:

113389004

API:

100167575

DNX:

107163428

AGS:

114120233

RMD:

113555196

BTAB:

109044264

DCI:	103506752 108253294

CLEC:

106670504

HHAL:

106683228

NLU:

111057072

PHU:	Phum_PHUM414590

FOC:

113212460

TPAL:

117645519

CSEC:

111873091

SGRE:

126271985 126317089

FCD:

110842788

DPX:	DAPPUDRAFT_332860

DMK:

116931070

DPZ:

124349493

PVM:

113818099

PJA:

122257684

PCHN:

125025607

PMOO:

119577269

HAME:

121875772

PCLA:

123765087

PTRU:

123518804

ESN:

126999011

HAZT:

108681612

EAF:

111696524

LSM:

121129872

PPOI:

119094250 119094251

ISC:	IscW_ISCW005296

DSV:

119440587

LPOL:

106463218

HRO:	HELRODRAFT_98504

LGI:	LOTGIDRAFT_157973

PCAN:

112553906

BGT:

106065027

GAE:

121385566

HRF:

124143730

HRJ:

124264477

CRG:

105326295

MYI:

110453744

PMAX:

117334218

MCAF:

127733788

MMER:

123566556

DPOL:

127875952

OBI:

106871734

OSN:

115209823

NVE:

5504006

EPA:

110252238

ATEN:

116294223

ADF:

107337999

AMIL:

114958545

PDAM:

113684402

SPIS:

111344377

DGT:	114523595 114523979

XEN:

124434950

HMG:

100208540

TAD:	TRIADDRAFT_19721

AQU:

100640903

MBR:	MONBRDRAFT_23152

SRE:	PTSG_04583

ACAN:

ACA1_063620

» show all

Reference

1 [PMID:9371691]

Authors

Qin YM, Haapalainen AM, Conry D, Cuebas DA, Hiltunen JK, Novikov DK.

Title

Recombinant 2-enoyl-CoA hydratase derived from rat peroxisomal multifunctional enzyme 2: role of the hydratase reaction in bile acid synthesis.

Journal

Biochem J 328 ( Pt 2):377-82 (1997)
DOI:10.1042/bj3280377

Reference

2 [PMID:8619845]

Authors

Xu R, Cuebas DA.

Title

The reactions catalyzed by the inducible bifunctional enzyme of rat liver peroxisomes cannot lead to the formation of bile acids.

Journal

Biochem Biophys Res Commun 221:271-8 (1996)
DOI:10.1006/bbrc.1996.0585

Reference

Authors

Kinoshita, T., Miyata, M., Ismail, S.M., Fujimoto, Y., Kakinuma, K., Kokawa, N.I. and Morisaki, M.

Title

Synthesis and determination of stereochemistry of four diastereoisomers at the C-24 and C-25 positions of 3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestan-26-oic acid and cholic acid.

Journal

Chem Pharm Bull 36:134-141 (1988)

Reference

Authors

Fujimoto, Y., Kinoshita, T., Oya, I., Kakinuma, K., Ismail, S.M., Sonoda, Y., Sato, Y. and Morisaki, M.

Title

Non-stereoselective conversion of the four diastereoisomers at the C-24 and C-25 positions of 3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestan-26-oic acid and cholic acid.

Journal

Chem Pharm Bull 36:142-145 (1988)

Reference

5 [PMID:11146090]

Authors

Kurosawa T, Sato M, Nakano H, Fujiwara M, Murai T, Yoshimura T, Hashimoto T.

Title

Conjugation reactions catalyzed by bifunctional proteins related to beta-oxidation in bile acid biosynthesis.

Journal

Steroids 66:107-14 (2001)
DOI:10.1016/S0039-128X(00)00217-8

Reference

6 [PMID:12543708]