Entry
Name
2-methylcitrate dehydratase (2-methyl-trans-aconitate-forming)
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
BRITE hierarchy
Sysname
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate hydro-lyase (2-methyl-trans-aconitate-forming)
Reaction(IUBMB)
(2S,3S)-2-methylcitrate = 2-methyl-trans-aconitate + H2O [RN:
R11263 ]
Reaction(KEGG)
Substrate
(2S,3S)-2-methylcitrate [CPD:
C02225 ]
Product
Comment
Catalyses the dehydration of (2S,3S)-2-methylcitrate, forming the trans isomer of 2-methyl-aconitate (unlike EC
4.2.1.79 , which forms only the cis isomer). Part of a propionate degradation pathway. The enzyme from Shewanella oneidensis can also accept citrate and cis-aconitate, but activity with (2S,3S)-2-methylcitrate was approximately 2.5-fold higher. 2-methylisocitrate and isocitrate were not substrates [1]. An iron-sulfur protein.
History
EC 4.2.1.117 created 2009
Pathway
Orthology
K20455 2-methylcitrate dehydratase (2-methyl-trans-aconitate forming)
Genes
XFN : XfasM23_0533 XfasM23_0534
XFF : XFLM_08000 XFLM_08005
XFU : XFF4834R_chr11330(acnA)
XPH : XppCFBP6546_05890(acnD)
XHD : LMG31886_32090(acnD)
LLZ : LYB30171_02030(acnD)
VAU : VANGNB10_cI1220(acnA)
VPL : SA104470976_01087(acnA)
PSYR : N018_09755 N018_17615
PVD : CFBP1590__2137(acnA)
PPRC : PFLCHA0_c18940(acnA1)
PMUD : NCTC8068_01566(acnD)
PFW : PF1751_v1c41380(acnA)
PPUU : PputUW4_03656(acnA2)
PSEC : CCOS191_1801(acnA1)
PBZ : GN234_00465 GN234_27395(acnD)
PVW : HU752_014970 HU752_024925(acnD)
PTRT : HU722_0021830(acnD)
PSHH : HU773_020120(acnD) HU773_024745
PFAK : KSS94_09600(acnD) KSS94_12620(acnA)
PTAE : NCTC10697_01188(acnD)
PSYC : DABAL43B_1466(acnA1)
MCUN : NCTC10297_00850(citB)
SALH : HMF8227_00163(acnA)
HAAH : HALA3H3_790092(acn)
HSR : HSBAA_41840 HSBAA_41870
ALCA : ASALC70_00056(acnD)
AEL : NCTC12917_02498(leuC_1)
NMM : NMBM01240149_1655(acnD)
NMS : NMBM01240355_0442(acnD)
NMQ : NMBM04240196_0440(acnD)
NMZ : NMBNZ0533_1816(acnD)
NMX : NMA510612_2314(acnA)
NWE : SAMEA3174300_1284(acnA)
NZO : SAMEA4504057_2050(acnA)
NCI : NCTC10296_00747(acnA)
RSM : CMR15_mp10089(acnA1)
BMN : BMA10247_A2141(acnM)
BPM : BURPS1710b_A1738(acnD)
BPL : BURPS1106A_A0290(acnD)
BPD : BURPS668_A0384(acnD)
BPZ : BP1026B_II0231(acnA)
BUM : AXG89_22815 AXG89_26290
PCAF : DSC91_002248 DSC91_003690
PDIO : PDMSB3_0793.1(acnD)
PLG : NCTC10937_02040(acnD_1)
TVL : FAZ95_15300(acnD) FAZ95_28585(acnA) FAZ95_32535(acnD)
BTRM : SAMEA390648701960(acnA1)
AXX : ERS451415_05484(acnA)
DEL : DelCs14_0738 DelCs14_3212
DHK : BO996_16220 BO996_27510
MUM : FCL38_05865(acnD) FCL38_28420(acnD)
ABS : AZOBR_p270030 AZOBR_p270031
» show all
Taxonomy
Reference
Authors
Grimek TL, Escalante-Semerena JC
Title
The acnD genes of Shewenella oneidensis and Vibrio cholerae encode a new Fe/S-dependent 2-methylcitrate dehydratase enzyme that requires prpF function in vivo.
Journal
Sequence
Other DBs
ExPASy - ENZYME nomenclature database: 4.2.1.117
LinkDB
All DBs