KEGG ENZYME: 4.2.1.120

ENZYME: 4.2.1.120

Entry

EC 4.2.1.120                Enzyme

Name

4-hydroxybutanoyl-CoA dehydratase

Class

Lyases;
Carbon-oxygen lyases;
Hydro-lyases

Sysname

4-hydroxybutanoyl-CoA hydro-lyase

Reaction(IUBMB)

4-hydroxybutanoyl-CoA = (E)-but-2-enoyl-CoA + H2O [RN:R10782]

Reaction(KEGG)

R10782

Substrate

4-hydroxybutanoyl-CoA [CPD:C11062]

Product

(E)-but-2-enoyl-CoA [CPD:C00877];
H2O [CPD:C00001]

Comment

Contains FAD and a [4Fe-4S] iron-sulfur cluster. The enzyme has been characterized from several microorganisms, including Clostridium kluyveri, where it participates in succinate fermentation [1,2], Clostridium aminobutyricum, where it participates in 4-aminobutyrate degradation [3,4], and Metallosphaera sedula, where it participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea [5].

History

EC 4.2.1.120 created 2009

Pathway

ec00650

Butanoate metabolism

ec00720

Carbon fixation pathways in prokaryotes

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K14534

4-hydroxybutyryl-CoA dehydratase / vinylacetyl-CoA-Delta-isomerase

Genes

BGJ:	AWC36_04165

BIZ:	HC231_14560

KAK:	Kalk_18475

AFUS:

EYZ66_08275

NAX:	HC341_03525

MGEO:

CFI10_04675

PNU:

Pnuc_0370

ACHR:

C2U31_22605

RHF:	EUB48_11505

POS:	DT070_18255

POO:	F7R28_12325

VPE:	Varpa_3723

VPD:	VAPA_1c23470

VAA:	AX767_00840

VBO:	CKY39_20160

VAM:	C4F17_02945

COF:	FOZ74_14275

RTA:

Rta_18760

HYB:

Q5W_11860

DIH:	G7047_12160

DRG:	H9K76_11775

SIMP:

C6571_16860

MELA:

C6568_04615

MPT:

Mpe_A2516

METP:

C1M51_16160

LCH:

Lcho_2993

GLC:	JQN73_05375

METR:

BSY238_78

APP:	CAP2UW1_1984

BRK:	CWS35_16070 CWS35_35405

BEL:	BE61_32540

BSEP:

HAP48_0025570

BQB:	J4P68_0010490

BAUT:

QA635_11525

BBRA:

QA636_10645

BGK:	IC762_28270

BPAH:

QA639_08730

OCA:

OCAR_6245

OCG:	OCA5_c17860(hbaB)

OCO:	OCA4_c17860(hbaB)

PCAX:

AFIC_003134

VGO:	GJW-30_1_00655(abfD)

BOI:	BLM15_19040

BVES:

QO058_18135

RHZ:	RHPLAN_22730

RBM:

TEF_19955

PSTG:

E8M01_28340

RID:	RIdsm_00769(abfD)

NOG:	GKE62_06270

SMAG:

AN936_12040

SMAZ:

LH19_13280

SPHQ:

BWQ93_08330

RDI:	CMV14_07515

SPHA:

D3Y57_03405

SJP:	SJA_C2-03820

SINB:

SIDU_11465

SPHJ:

BSL82_04790

SRF:	LHU95_19065

DEX:	HWD60_00500

DVN:	HQ394_03595

DAV:	DESACE_06790

GME:

Gmet_2215

TAMM:

GEAMG1_2143(abfD)

DEU:	DBW_0157(abfD)

DOL:

Dole_2592

DAL:	Dalk_0576 Dalk_1215 Dalk_2536 Dalk_2731 Dalk_3391

DTO:	TOL2_C28460(hpaB)

DWD:	DSCW_05650(abfD_1) DSCW_20400 DSCW_31010(abfD_2) DSCW_33530 DSCW_58950 DSCW_59050

DALK:

DSCA_29960(abfD)

DMM:	dnm_056620

DEK:	DSLASN_12450(abfD)

SAT:

SYN_02445

DTI:	Desti_0809 Desti_1603 Desti_2004 Desti_3009 Desti_3036

SFU:	Sfum_3141 Sfum_3932

DBR:	Deba_0626 Deba_1051

SAMY:

DB32_005276

NANN:

O0S08_40440

BACO:

OXB_3219

EFF:	skT53_09470

CBE:

Cbei_2100

CBZ:

Cbs_2100

CBEI:

LF65_02412

CKL:	CKL_3020(abfD)

CKR:

CKR_2668

CLS:	CXIVA_14640

CPAS:

Clopa_4009

CSQ:	CSCA_3616 CSCA_3622

CCK:	Ccar_01580

CTYK:

CTK_C07640(abfD)

CEU:	A7L45_11985

CDRK:

B9W14_01580

CDY:	F3K33_10480

CFER:

D4Z93_08285 D4Z93_11490

CCAA:

KQH81_12640

CTAG:

LL095_09905

CRS:	FQB35_09340

CPRF:

K7H06_04670 K7H06_04690

CRAS:

KVH43_00880 KVH43_00895

FPLA:

A4U99_00480

RGV:	NQ502_13955 NQ502_18195

CAPR:

EQM14_06625 EQM14_09375 EQM14_10020

OVA:	OBV_01110(abfD) OBV_03760(abfD) OBV_28840(abfD) OBV_33370(abfD) OBV_35330(abfD) OBV_40730(abfD)

OBJ:	EIO64_09505

VFA:	MM35RIKEN_12830(abfD)

AGAT:

RWV98_04230 RWV98_06155 RWV98_10260

BPRO:

PMF13cell1_01502(abfD)

LACY:

A4V08_31375

LXA:	OW255_07515

ARF:	AR1Y2_2847 AR1Y2_3067

ACAC:

EYQ97_04915

WHJ:	H9Q79_06930 H9Q79_08895 H9Q79_10120

CDF:	CD630_23410(abfD)

PDC:	CDIF630_02580(abfD)

CDC:	CD196_2184(abfD)

CDL:	CDR20291_2230(abfD)

PDF:	CD630DERM_23410(abfD)

CMIC:

caldi_15750

SLP:

Slip_0662

DSY:

DSY4732

DDH:	Desde_1768

DMT:	DESME_04405

DOR:	Desor_0848 Desor_0856 Desor_0867 Desor_2541 Desor_3713

DAI:	Desaci_1066 Desaci_1680

DMI:	Desmer_0870 Desmer_3350

PTH:	PTH_1222 PTH_1770

TFR:	BR63_00565 BR63_09495 BR63_10065 BR63_10755 BR63_16155 BR63_17815 BR63_17890

FWA:	DCMF_17520 DCMF_19260 DCMF_24550 DCMF_27885

AACX:

DEACI_0207 DEACI_1870

ELM:

ELI_0281

EMT:	CPZ25_012290

ELIM:

B2M23_04455

INT:	RX717_00835 RX717_03935

CMIU:

B1H56_01400 B1H56_13815

IBU:	IB211_00283 IB211_01887c

AMIJ:

EQM06_00080 EQM06_05020

ABUT:

Ami103574_00850

ETM:	CE91St48_13840(abfD_1) CE91St48_25600(abfD_2) CE91St48_30890 CE91St48_33910(abfD_3)

CHY:	CHY_1314(abfD) CHY_1730

PED:	ING2D1G_1210(abfD)

SPOA:

EQM13_05475

MED:

MELS_0342

MHW:	ACT01_02930 ACT01_03000 ACT01_03330 ACT01_05095 ACT01_09475

MEG:	DKB62_02945 DKB62_07650

STED:

SPTER_02600(abfD)

AIN:

Acin_2034

ERZ:	ER308_02395

MID:

MIP_03610

MCHI:

AN480_13630

MMAL:

CKJ54_11700

MDX:	BTO20_21570

ROA:	Pd630_LPD17032

RGO:	KYT97_22845

GPO:	GPOL_c49520

GTA:	BCM27_04235

GAM:	GII34_07415

SLE:	sle_61260(sle_61260)

SFEU:

IM697_23390

ASUF:

MNQ99_17175 MNQ99_17230

NANO:

G5V58_14050

TCU:

Tcur_0955

MCRA:

ID554_30515

VER:	HUT12_14055

DVC:	Dvina_12895

DMAT:

Dmats_12455

TFLA:

O0235_14865

TRM:	JO41_12025

TRZ:	GWP43_07785

TMED:

DWB79_09475

TRP:	ABH09_07335

FNC:	HMPREF0946_00150

FNT:	HMPREF0405_01111

FVA:	FV113G1_21520

FUL:	C4N20_16050

FNF:	BSQ88_08665

FCI:	I6I83_08420

PGI:	PG_0692(abfD)

PGN:

PGN_0727

PGT:	PGTDC60_1817(abfD)

PAH:	Poras_0448

PCRE:

NCTC12858_01801(abfD)

PCAG:

NCTC12856_01249(abfD)

PEND:

LA319_00265

PSOE:

CE91St14_12610(abfD)

TFO:	BFO_1484(abfD)

OSP:	Odosp_2062

BUY:	D8S85_11120

BVIR:

I6J59_04675

RSI:	Runsl_1400

RUN:	DR864_15875

SHYR:

LA303_00675

TTK:	TST_0398(abfD)

AFU:	AF_0333 AF_1027

AFG:	AFULGI_00003320 AFULGI_00011230

AST:	Asulf_01733

FPL:

Ferp_0911

GAC:	GACE_0594 GACE_1499

GAH:

GAH_01688

FAC:	FACI_IFERC01G0375

FAI:

FAD_1294

IHO:

Igni_0595

IIS:

EYM_00450

IPC:

IPA_02940

PFM:	Pyrfu_1275

STO:	STK_00630 STK_16590

SOH:	D1869_09350 D1869_14735

SSO:	SSO2503(abfD-1) SSO2738(abfD-2)

SOL:	Ssol_0312 Ssol_0550

SSOA:

SULA_0305 SULA_0541

SSOL:

SULB_0307 SULB_0543

SSOF:

SULC_0305 SULC_0541

SSHI:

J5U23_00761 J5U23_01288

SCAS:

SACC_14040 SACC_17730 SACC_24830

SAI:	Saci_1103 Saci_2143(abfD)

SACN:

SacN8_05365 SacN8_10430

SACR:

SacRon12I_05355 SacRon12I_10685

SACS:

SUSAZ_05145 SUSAZ_09780

SIS:	LS215_0322 LS215_2744

SIA:	M1425_0293 M1425_2585

SIM:	M1627_0293 M1627_2638

SID:	M164_0311 M164_2569

SIY:	YG5714_0299 YG5714_2751

SIN:	YN1551_0139 YN1551_2902

SII:	LD85_0299 LD85_2896

SIH:	SiH_0300 SiH_2522

SIR:	SiRe_0298 SiRe_2362

SIC:	SiL_0284 SiL_2419

SULA:

BFU36_06625 BFU36_11260

SULE:

GFS03_00420 GFS03_03030

SULO:

GFS33_05550 GFS33_08205

SULL:

EWF20_01055 EWF20_10955

MSE:	Msed_1220 Msed_1321

MEMJ:

MJ1HA_1382 MJ1HA_1530

MCN:

Mcup_0888

MHK:	DFR87_12060

MPRU:

DFR88_00405 DFR88_11715

MTEN:

GWK48_00525 GWK48_10830

MJN:	MjAS7_2228 MjAS7_2390

AHO:

Ahos_2036

AMAN:

B6F84_01555 B6F84_05320

ABRI:

DFR85_01140 DFR85_12315

ASUL:

DFR86_01545

AAMB:

D1866_05205

ACIH:

HS5_08570

SAZO:

D1868_09500

CSTY:

KN1_05100

STEP:

IC006_0828

SMET:

RQ359_001001

PAI:

PAE2693

PIS:

Pisl_0248

PCL:

Pcal_1396

PYR:

P186_0718

POG:

Pogu_1298

TNE:

Tneu_0422

PYW:	PYWP30_00440

TTN:

TTX_1102

TUZ:

TUZN_0949

VDI:

Vdis_2214

VMO:	VMUT_0681 VMUT_2253

VSO:	Vsou_04700 Vsou_16100 Vsou_20760

CLG:	Calag_0024

NMR:

Nmar_0207

NIR:	NSED_00835

NKR:	NKOR_00855

NID:	NPIRD3C_0596(abfD)

NIN:	NADRNF5_2050(abfD)

NIW:	Nisw_03935

NCL:	C5F47_01015

NOX:	C5F49_01000

NUE:	C5F50_01330

NIP:	NsoK4_07890

NZT:	NZOSNM25_001871

NCT:

NMSP_1505

NIC:	DSQ20_01275

NGA:	Ngar_c34390

NVN:	NVIE_017160

NEV:

NTE_00645

TAA:	NMY3_03315(abfD)

NFN:	NFRAN_0724(hcd)

NCV:	NCAV_0126 NCAV_0127

CSY:	CENSYa_0022

NBV:	T478_1248(abfD)

TAH:	SU86_007675

NIU:	DSQ19_09180

NDV:	NDEV_0181(hcd)

BARC:

AOA65_2031 AOA65_2222

LOKI:

Lokiarch_00630(abfD_1) Lokiarch_26350(abfD_2)

» show all

Reference

1 [PMID:13687513]

Authors

BARTSCH RG, BARKER HA

Title

A vinylacetyl isomerase from Clostridium kluyveri.

Journal

Arch Biochem Biophys 92:122-32 (1961)
DOI:10.1016/0003-9861(61)90226-0

Reference

2 [PMID:8161284]

Authors

Scherf U, Sohling B, Gottschalk G, Linder D, Buckel W

Title

Succinate-ethanol fermentation in Clostridium kluyveri: purification and characterisation of 4-hydroxybutyryl-CoA dehydratase/vinylacetyl-CoA delta 3-delta 2-isomerase.

Journal

Arch Microbiol 161:239-45 (1994)
DOI:10.1007/bf00248699

Sequence

[ckl:CKL_3020]

Reference

3 [PMID:8344309]

Authors

Scherf U, Buckel W

Title

Purification and properties of an iron-sulfur and FAD-containing 4-hydroxybutyryl-CoA dehydratase/vinylacetyl-CoA delta 3-delta 2-isomerase from Clostridium aminobutyricum.

Journal

Eur J Biochem 215:421-9 (1993)
DOI:10.1111/j.1432-1033.1993.tb18049.x

Sequence

[ag:CAB60035]

Reference

4 [PMID:8794752]

Authors

Muh U, Cinkaya I, Albracht SP, Buckel W

Title

4-Hydroxybutyryl-CoA dehydratase from Clostridium aminobutyricum: characterization of FAD and iron-sulfur clusters involved in an overall non-redox reaction.

Journal

Biochemistry 35:11710-8 (1996)
DOI:10.1021/bi9601363

Reference

5 [PMID:18079405]

Authors

Berg IA, Kockelkorn D, Buckel W, Fuchs G

Title

A 3-hydroxypropionate/4-hydroxybutyrate autotrophic carbon dioxide assimilation pathway in Archaea.

Journal

Science 318:1782-6 (2007)
DOI:10.1126/science.1149976

Other DBs

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