KEGG ENZYME: 4.2.1.132

ENZYME: 4.2.1.132

Entry

EC 4.2.1.132                Enzyme

Name

2-hydroxyhexa-2,4-dienoate hydratase;
tesE (gene name);
hsaE (gene name)

Class

Lyases;
Carbon-oxygen lyases;
Hydro-lyases

Sysname

4-hydroxy-2-oxohexanoate hydro-lyase [(2Z,4Z)-2-hydroxyhexa-2,4-dienoate-forming]

Reaction(IUBMB)

4-hydroxy-2-oxohexanoate = (2Z,4Z)-2-hydroxyhexa-2,4-dienoate + H2O [RN:R09960]

Reaction(KEGG)

R09960;
(other) R05864

Substrate

4-hydroxy-2-oxohexanoate [CPD:C06762]

Product

(2Z,4Z)-2-hydroxyhexa-2,4-dienoate [CPD:C20240];
H2O [CPD:C00001]

Comment

This enzyme catalyses a late step in the bacterial steroid degradation pathway. The product, 4-hydroxy-2-oxohexanoate, forms a 2-hydroxy-4-hex-2-enolactone under acidic conditions.

History

EC 4.2.1.132 created 2012

Pathway

ec00622

Xylene degradation

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K18364

2-oxopent-4-enoate/cis-2-oxohex-4-enoate hydratase

Genes

PGE:	LG71_12240

PLUM:

A4R40_11035

PAKH:

B0X70_11350

PLUI:

CE143_11265

PSD:	DSC_09160 DSC_09365

LSY:	H8L67_00470(dmpE)

RGL:	CS053_01760

RTH:	LRK53_10275(dmpE)

RBD:

ALSL_1080

VAF:	D1115_22450

PRE:	PCA10_13820 PCA10_p0350(carD)

PPSE:

BN5_3620(nahL)

PFUW:

KF707C_12170 KF707C_15560(bzaF) KF707C_16070(salF) KF707C_16250(bphX1)

PCQ:	PcP3B5_25700(tesE_1)

PNT:	G5B91_07425(dmpE)

PPI:	ND086(nahL)

PPUT:

L483_14065

PPUN:

PP4_27260

PPJ:	RK21_04237

PZA:	HU749_013350

PVR:	PverR02_11595 PverR02_14690

PMED:

E3Z27_14780

PPUU:

PputUW4_01660(mhpD)

PKC:	PKB_4239(xylJ)

PFZ:	AV641_10535

PLQ:	AA042_19765

PALK:

PSAKL28_05940

PPSY:

AOC04_07835

PFK:	PFAS1_28775

PSET:

THL1_1433

PSIL:

PMA3_11735 PMA3_18070

PKE:	DLD99_14750

PALL:

UYA_06720 UYA_06980 UYA_07060 UYA_20155

PUM:	HGP31_20760

PPSH:

G5J76_13490

PLAL:

FXN65_06420

PAZE:

KSS91_19295

PALV:

KSS97_16725

PTAE:

NCTC10697_02057(xylJ)

PCAV:

D3880_02345(dmpE) D3880_11810(dmpE) D3880_17505

PPHN:

HU825_04980(dmpE) HU825_14755

PIZ:	LAB08_R28780(dmpE) LAB08_R40970

PKM:	PZ739_11545

PSHS:

JJN09_05425

PLAU:

I0D68_00650(dmpE) I0D68_06180 I0D68_15095(dmpE)

PFOU:

OZ911_14080

PBEJ:

PSH84_16905

PSC:	A458_06520

PSTU:

UIB01_22740

PBM:	CL52_14710

SDEG:

GOM96_01760

AVN:	Avin_08670(xylJ) Avin_30610(lapE)

AVL:	AvCA_08670(xylJ) AvCA_30610(lapE)

AVD:	AvCA6_08670(xylJ) AvCA6_30610(lapE)

ACX:	Achr_32780

HPEG:

EAO82_00555(dmpE) EAO82_09425(dmpE)

HAES:

LO767_17670(dmpE)

HARR:

HV822_17975

PPHA:

BVH74_06155 BVH74_16350 BVH74_17960

MAD:	HP15_1113 HP15_4034

MSR:	AU15_21080

MLQ:	ASQ50_09215

MSQ:	BKP64_13850

MARJ:

MARI_05440(xylJ)

MALL:

PBN92_01670(dmpE)

MSHE:

MAALD49_03410 MAALD49_03860

MANP:

EHN06_02445(dmpE)

KAK:	Kalk_07855

SPL:

Spea_2118

SHL:

Shal_2090

SWD:

Swoo_1392

COLW:

A3Q33_18575

CBER:

B5D82_11270

THT:	E2K93_11200

PHA:	PSHAa2141(xylJ)

PTN:	PTRA_a2594(bphH)

PNG:	PNIG_a2788(bphH)

SPOI:

IMCC21906_00389 IMCC21906_02710

SNAN:

I6N98_10800

STAW:

NCG89_14300

ZAL:	AZF00_03890 AZF00_11430 AZF00_17820

HJA:	BST95_09505

HALC:

EY643_13295

AFUS:

EYZ66_02605(dmpE) EYZ66_12965

PAQM:

E0F26_07475

CYQ:

Q91_0508

CZA:	CYCME_2103

CYY:	CPC19_10515(dmpE)

BLEP:

AL038_01300(dmpE)

THIS:

HZT40_10605

AEH:

Mlg_2460

TBN:

TBH_C0928

APAC:

S7S_06990

MGEO:

CFI10_00425(dmpE) CFI10_04960(dmpE)

MSEC:

LN244_04775 LN244_11330(dmpE)

MRZ:	KDW95_00770

NCU:	F0U83_03045(dmpE)

NJP:	NEJAP_0671(mhpD)

NIK:	F5I99_10105(dmpE) F5I99_10520(dmpE)

AJP:	AMJAP_2999(mhpD)

EMP:	EZMO1_1317(tesE)

ASIM:

FE240_03500(dmpE)

ZDF:	AN401_14585

SOK:	D0B54_06200

SINI:

GT972_13730(dmpE) GT972_14670

FCE:	JN531_005830

GBI:	PG2T_02230

GPB:	HDN1F_27090(tesE)

NZO:	SAMEA4504057_0450(xylJ)

NCI:	NCTC10296_00911(xylJ_1)

NWD:	H3L96_04745(dmpE)

NDU:	LVJ88_00610(dmpE)

VFF:	VITFI_CDS1370

PTES:

JQU52_04880(dmpE)

USU:	LVJ78_03680(dmpE)

CHRO:

CXB49_03840

AQL:	BXU06_16875

RPI:

Rpic_4620

RPF:	Rpic12D_3544

RPJ:	N234_08150 N234_12215

RIN:	ACS15_4354(dmpE)

REH:	H16_B0548(mhpD1) H16_B0597(bphH)

CNC:	CNE_2c04940(mhpD) CNE_2c05420(xylJ2) CNE_BB1p11990(xylJ1) CNE_BB1p13280(xylJ2)

CUH:	BJN34_30960

REU:	Reut_A1510 Reut_B5691

RME:	Rmet_1321(xylJ)

CTI:	RALTA_B0401(mhpD)

CBW:	RR42_s2313 RR42_s2484

CCUP:

BKK81_19910 BKK81_27090

CUP:	BKK80_21900 BKK80_29230

CUU:	BKK79_35180

COX:	E0W60_07775(dmpE) E0W60_08025(dmpE)

CCAX:

KZ686_09995(dmpE)

CUK:	KB879_20190(dmpE) KB879_20425(dmpE)

CNAN:

A2G96_22770

BVI:	Bcep1808_7603

BUR:	Bcep18194_B1459 Bcep18194_B2961

BCJ:

BCAM1613

BCEN:

DM39_3383(dmpE) DM39_4834(dmpE)

BCEO:

I35_5473

BCT:	GEM_4111 GEM_5403

BCED:

DM42_4993(dmpE) DM42_6345(dmpE)

BCEP:

APZ15_31155

BPYR:

ABD05_27820

BCON:

NL30_04140 NL30_24600

BDF:	WI26_15985 WI26_21145

BTEI:

WS51_00560 WS51_05695

BPSL:

WS57_06045 WS57_12165

BMEC:

WJ16_26790

BSTL:

BBJ41_10510 BBJ41_21340

BANN:

JFN94_19125(dmpE) JFN94_25650(dmpE)

BARI:

NLX30_30185(dmpE)

BAEN:

L3V59_21740(dmpE) L3V59_30395(dmpE)

BUQ:	AC233_11195

BURK:

DM992_32625(dmpE)

BXE:	Bxe_C1189(bphH)

BXB:	DR64_8616(dmpE)

BUZ:	AYM40_21220

BFN:	OI25_4589(dmpE)

PARB:

CJU94_37870(dmpE) CJU94_38115(dmpE)

PCAF:

DSC91_003291

PMEG:

FNZ07_21165(dmpE)

PBRY:

NDK50_32875(dmpE)

PPAI:

E1956_37240(dmpE) E1956_45885

PHUN:

U0042_26770(dmpE)

PPK:	U875_01900

PRB:	X636_07570

PVE:	UC34_07065

CABA:

SBC2_49020(tesE)

LIMN:

HKT17_03820(dmpE)

BOJ:	CBF45_01490 CBF45_04500 CBF45_06955

APES:

FOC84_00320(dmpE)

CDN:	BN940_01166 BN940_04316

KGY:	EHF36_01675(dmpE) EHF36_04885(dmpE)

RLH:	MIZ03_0646 MIZ03_1022

PNA:

Pnap_4144

AJS:

Ajs_0222

ACID:

CBP33_01105

ACIP:

CBP36_01200

ACIN:

CBP34_01110

ACIS:

CBP35_17750

AMON:

H9L24_14185(dmpE)

VEI:

Veis_2785

VBO:	CKY39_28505(dmpE)

VAV:	IG196_08130(dmpE)

CTT:	CtCNB1_1353 CtCNB1_3149

CTES:

O987_07740

COF:	FOZ74_08305(dmpE)

CRJ:	QMY55_07020

ADN:	Alide_0337

ADK:	Alide2_0282

OTD:	J1M35_00055(dmpE)

HYL:	LPB072_18180

HPSE:

HPF_01870(xylJ)

HYN:	F9K07_02750(dmpE)

HTN:	KI616_01965(dmpE)

DIH:	G7047_01670(dmpE) G7047_09705

DRG:	H9K76_11645(dmpE)

SIMP:

C6571_13615(dmpE)

MELA:

C6568_07860(dmpE)

MJE:	LVC68_01005(dmpE)

STHM:

IS481_10530(dmpE)

SAQA:

OMP39_09110(dmpE)

MPT:	Mpe_A2273 Mpe_A3325

METP:

C1M51_06460(dmpE) C1M51_15400(dmpE)

SNN:	EWH46_19325

SMIO:

CATMQ487_04110 CATMQ487_17560 CATMQ487_31610

IDC:	LRM40_19570(dmpE)

LCH:

Lcho_3343

JAG:	GJA_236(xylJ)

JAJ:	EKL02_01815(dmpE)

HEW:	HBDW_03980

MASW:

AM586_18650

TMJ:	P0M04_30675(dmpE)

ACTB:

RHM62_03640

RBU:	PG1C_14105

ABRE:

pbN1_20560(mhpD)

DAR:	Daro_2774 Daro_3785

DEY:	HYN24_14130(dmpE) HYN24_14215(dmpE)

DDEN:

KI615_16885(dmpE)

FLE:	KI610_08575(dmpE) KI610_13420(dmpE) KI610_15465(dmpE)

AZO:	azo1853(lapE) azo2434(nahL)

AOA:	dqs_2006 dqs_2578

AZI:	AzCIB_1507 AzCIB_4411

AZD:	CDA09_09955 CDA09_10325(dmpE) CDA09_10440(dmpE) CDA09_21440(dmpE)

AZR:	CJ010_12250(dmpE) CJ010_15220(dmpE)

TMZ:	Tmz1t_2077 Tmz1t_3091

THU:	AC731_001810 AC731_008895

THAU:

C4PIVTH_2807(mhpD) C4PIVTH_4321(mhpD) C4PIVTH_4380(mhpD)

THK:	CCZ27_10770(dmpE) CCZ27_16275(dmpE)

THAG:

CKCBHOJB_02379(tesE_1) CKCBHOJB_03304(tesE_2)

AZQ:	G3580_00535(dmpE) G3580_08270(dmpE)

RPOP:

K1718_22490

PAQT:

E8L99_04850

TEC:	AKL02_008255

NAR:

Saro_3689

NPP:	PP1Y_AT32745 PP1Y_Mpl3103

NPN:	JI59_22525

NOV:	TQ38_027295

NOT:	C7W88_20925

SGI:	SGRAN_1581(mhpD)

SPHM:

G432_01985

SSAN:

NX02_12710

SPKC:

KC8_03560

SPHA:

D3Y57_15215

SLAN:

GV829_01855

SBIN:

SBA_ch2_4290

SJP:	SJA_C1-11870(mhpD)

SINB:

SIDU_08330

SYB:	TZ53_09010 TZ53_23455

SPHB:

EP837_03394(bphH)

SHYD:

CJD35_06490

SUFL:

FIL70_19005

SBAR:

H5V43_20655

SFLA:

SPHFLASMR4Y_02368(tesE)

SPHJ:

BSL82_10455

PALG:

HFP57_16665

PHAO:

HF685_05520

ALTN:

LBX01_06760

PMAS:

NCF86_10595

TFV:	IDJ81_04970

PHZ:	CHX26_05320

NTD:	EGO55_13680

CAEN:

K5X80_14735

PEG:	E5R92_01705

HTL:

HPTL_0050

ACIB:

ACBT_0261(dmpE)

AFC:	AFAEC_1846(dmpE)

AAQI:

AAQM_0980(dmpE)

ACLO:

ACLO_a0141(dmpE)

ADZ:	ADFLV_2553(dmpE)

ARC:

ABLL_2444

AROS:

NJU99_01640(dmpE)

ALK:	ALEK_0381(dmpE)

AANA:

AANAER_1258(dmpE)

ASED:

IRT44_17630

BHUI:

LOK74_00600

BAYD:

BSPP4475_07455

BBOR:

RFB14_22165

AFAS:

NZD89_10100

FFE:	LSG31_21095

MHAP:

JFY71_09925

CFEU:

CFELI_13980(mhpD)

ACTR:

Asp14428_49240

CWO:

Cwoe_3997

PARN:

NBH00_20090

AMOB:

HG15A2_23830(tesE)

NAY:	HYG81_22605

» show all

Reference

1 [PMID:16151114]

Authors

Horinouchi M, Hayashi T, Koshino H, Kurita T, Kudo T

Title

Identification of 9,17-dioxo-1,2,3,4,10,19-hexanorandrostan-5-oic acid, 4-hydroxy-2-oxohexanoic acid, and 2-hydroxyhexa-2,4-dienoic acid and related enzymes involved in testosterone degradation in Comamonas testosteroni TA441.

Journal

Appl Environ Microbiol 71:5275-81 (2005)
DOI:10.1128/AEM.71.9.5275-5281.2005

Sequence

[ag:BAC67694]

Other DBs

ExplorEnz - The Enzyme Database:

4.2.1.132

IUBMB Enzyme Nomenclature:

4.2.1.132

ExPASy - ENZYME nomenclature database:

4.2.1.132

UM-BBD (Biocatalysis/Biodegradation Database):

4.2.1.132

BRENDA, the Enzyme Database:

4.2.1.132

LinkDB

DBGET integrated database retrieval system