Entry
Name
2-methylcitrate dehydratase;
2-methylcitrate hydro-lyase;
PrpD;
2-hydroxybutane-1,2,3-tricarboxylate hydro-lyase
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
BRITE hierarchy
Sysname
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate hydro-lyase [(Z)-but-2-ene-1,2,3-tricarboxylate-forming]
Reaction(IUBMB)
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate = (Z)-but-2-ene-1,2,3-tricarboxylate + H2O [RN:
R04424 ]
Reaction(KEGG)
Substrate
(2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate [CPD:
C02225 ]
Product
(Z)-but-2-ene-1,2,3-tricarboxylate [CPD:
C04225 ];
H2O [CPD:
C00001 ]
Comment
The enzyme is specific for (2S,3S)-methylcitrate, showing no activity with (2R,3S)-methylcitrate [2]. The enzyme can also use cis-aconitate as a substrate but more slowly [2]. Both this enzyme and EC
4.2.1.3 , aconitate hydratase, are required to complete the isomerization of (2S,3S)-methylcitrate to (2R,3S)-2-methylisocitrate [2].
History
EC 4.2.1.79 created 1984
Pathway
Orthology
K01720 2-methylcitrate dehydratase
Genes
CGR : 2891103(GVI51_L09053)
NCS : NCAS_0B04690(NCAS0B04690)
NDI : NDAI_0B02100(NDAI0B02100)
TPF : TPHA_0P00490(TPHA0P00490)
TBL : TBLA_0C05240(TBLA0C05240)
TDL : TDEL_0C01220(TDEL0C01220)
KAF : KAFR_0B01840(KAFR0B01840)
KNG : KNAG_0D02470(KNAG0D02470)
YLI : 2908117(YALI2_F00042g)
NTE : NEUTE1DRAFT118571(NEUTE1DRAFT_118571)
SMP : 10810152(SMAC4_01359)
TATV : 25778068(TrAtP1_001959)
PLJ : 28882549(PLICBS_001433)
CDET : 87944579(CDEST_08076)
PFY : PFICI_09704 PFICI_09865
SSL : SS1G_00243 SS1G_01976 SS1G_05127
BFU : BCIN_07g03110 BCIN_09g01930(Bcpdh1)
AFM : AFUA_2G13610 AFUA_6G03730
NFI : NFIA_050250 NFIA_088800
AOR : AO090010000155 AO090026000253 AO090701000175
ANG : An01g09930 An08g10870 An15g01780
AFV : AFLA_004674 AFLA_006043 AFLA_013699
ALUC : AKAW2_21033A AKAW2_21156A AKAW2_52044A AKAW2_60519A(PDH1)
ACHE : ACHE_50113S(PDH1) ACHE_50807S
PCS : N7525_001393 N7525_009714
PDP : PDIP_17020 PDIP_79090
PTRR : 6343641(PtrM4_110820)
BZE : COCCADRAFT_39676 COCCADRAFT_90234
BOR : COCMIDRAFT_104204 COCMIDRAFT_107996
NPA : UCRNP2_7880 UCRNP2_7997
ABP : AGABI1DRAFT83674(AGABI1DRAFT_83674)
ABV : AGABI2DRAFT135920(AGABI2DRAFT_135920)
SCM : SCHCO_02632757(SCHCODRAFT_02632757)
PSTR : Pst134EA_022613 Pst134EA_022620
ECOC : C3026_01635(prpD) C3026_24805(prpD)
ECOO : ECRM13514_0517(prpD)
ECOH : ECRM13516_0311(prpD)
ELR : ECO55CA74_02125(prpD)
ECW : EcE24377A_0358(prpD)
ECOA : APECO78_05190(prpD)
EDJ : ECDH1ME8569_0321(prpD)
ECOI : ECOPMV1_00340(prpD)
SEM : STMDT12_C04320(prpD)
SETC : CFSAN001921_15195(prpD)
SEEN : SE451236_07860(prpD)
SENH : CFSAN002069_07000(prpD)
SEEH : SEEH1578_11270(prpD)
SENJ : CFSAN001992_09345(prpD)
SEEC : CFSAN002050_08440(prpD)
SEEB : SEEB0189_017460(prpD)
SENC : SEET0819_08805(prpD)
PSHI : SAMEA2665130_1486(prpD)
SMW : SMWW4_v1c14460(prpD)
SERS : SERRSCBI_06865(prpD)
SFJ : SAMEA4384070_1515(prpD)
PHEI : NCTC12003_01322(prpD)
PRJ : NCTC6933_02690(prpD)
LLZ : LYB30171_02028(prpD)
RHD : R2APBS1_1550 R2APBS1_2542
RGL : CS053_01925 CS053_12610
VNI : VIBNI_A1830(prpD) VIBNI_B0519
PAEB : NCGM1900_1984(prpD)
PPRC : PFLCHA0_c18960(prpD)
PMUD : NCTC8068_01568(prpD)
PFW : PF1751_v1c41360(prpD)
PFF : PFLUOLIPICF715495(prpD)
PTRT : HU722_0021820(prpD)
PTAE : NCTC10697_01190(prpD)
PSYC : DABAL43B_1473(prpD)
PLZ : S4054249_08165(prpD)
SALH : HMF8227_01407(prpD)
MICC : AUP74_02453(prpD_1)
CBS : COXBURSA331_A1032(prpD)
LLG : 44548918_01497(prpD)
LJR : NCTC11533_01459(prpD)
LCJ : NCTC11976_02198(prpD)
LWA : SAMEA4504053_1761(prpD)
LSS : NCTC12082_00604(prpD)
LADL : NCTC12735_00655(prpD)
LANT : TUM19329_19030(prpD)
NWR : E3U44_13140 E3U44_17250
TFRI : Thiofri_04765(prpD)
TNI : TVNIR_3342(prpD_[H])
HAAH : HALA3H3_790094(prpD)
ALCA : ASALC70_00058(prpD)
ADI : B5T_02357(prpD) B5T_03082
IGN : MMG00_11465 MMG00_12660
RPJ : N234_09130 N234_11055(prpD) N234_23230
RIN : ACS15_1165(prpD) ACS15_3730
CCUP : BKK81_14975 BKK81_24625
CUP : BKK80_15925 BKK80_31710
CUU : BKK79_17025 BKK79_22620
BMA : BMAA1754(prpD) BMAA1953
BMV : BMASAVP1_0970 BMASAVP1_1616(prpD)
BML : BMA10229_1256 BMA10229_1823(prpD)
BMN : BMA10247_A0494(prpD) BMA10247_A2231
BMAL : DM55_3329(prpD) DM55_3838
BMAE : DM78_4757 DM78_4915(prpD)
BMAQ : DM76_4027(prpD) DM76_4535
BMAI : DM57_06295 DM57_07225(prpD)
BMAF : DM51_3448(prpD) DM51_3637
BMAZ : BM44_3266(prpD) BM44_3798
BMAB : BM45_4104(prpD) BM45_4572
BPS : BPSS1725(prpD) BPSS2161
BPM : BURPS1710b_A0799(prpD) BURPS1710b_A1275
BPL : BURPS1106A_A2339(prpD) BURPS1106A_A2918
BPD : BURPS668_A2477(prpD) BURPS668_A3042
BPSE : BDL_5079(prpD) BDL_5629
BPSM : BBQ_3950 BBQ_4415(prpD)
BPSU : BBN_5180(prpD) BBN_5648
BPSD : BBX_4245(prpD) BBX_4796
BPZ : BP1026B_II1846(prpD) BP1026B_II2326
BPQ : BPC006_II2312 BPC006_II2872
BPK : BBK_4444(prpD) BBK_4934
BPSH : DR55_3543(prpD) DR55_5575
BPSA : BBU_3834 BBU_4300(prpD)
BPSO : X996_5347 X996_5820(prpD)
BUT : X994_4895 X994_5381(prpD)
BTE : BTH_II0655(prpD) BTH_II2237
BTQ : BTQ_3947(prpD) BTQ_5525
BTJ : BTJ_4181 BTJ_4979(prpD)
BTZ : BTL_3447(prpD) BTL_4991
BTD : BTI_4236(prpD) BTI_4580
BTV : BTHA_4438(prpD) BTHA_5231
BTHE : BTN_4649 BTN_5434(prpD)
BTHM : BTRA_4916 BTRA_5709(prpD)
BTHA : DR62_4756 DR62_5611(prpD)
BTHL : BG87_3402(prpD) BG87_4981
BHG : I6G56_30205 I6G56_31870
BUD : AQ610_20405 AQ610_22020(prpD)
BUR : Bcep18194_B1723 Bcep18194_B2157
BCEP : APZ15_19640(prpD) APZ15_35195
BPYR : ABD05_16990(prpD) ABD05_32515
BUB : BW23_3871(prpD) BW23_4132 BW23_4685(prpD)
BTEI : WS51_01400 WS51_03080(prpD)
BSTG : WT74_21060 WT74_23975(prpD)
BARI : NLX30_33635 NLX30_34090
BAEN : L3V59_20295 L3V59_34610
BGD : bgla_2g02660 bgla_2g18360
BYI : BYI23_B009460 BYI23_B011930
BUE : BRPE67_BCDS13540 BRPE67_BCDS15710
BUL : BW21_3776(prpD) BW21_4102
BGP : BGL_2c18250(prpD1) BGL_2c20420(prpD2)
BPLA : bpln_2g19020 bpln_2g20670
BUM : AXG89_13030 AXG89_19940(prpD) AXG89_21690(prpD)
BUI : AX768_22855(prpD) AX768_24225(prpD)
BURK : DM992_30495(prpD) DM992_38765
BCAI : K788_0000481 K788_0000944 K788_0004381
PHS : C2L64_31985(prpD) C2L64_41065
PTER : C2L65_28720(prpD) C2L65_37955
PMEG : FNZ07_06010 FNZ07_07390
PDIO : PDMSB3_0147.1(prpD)
PSAA : QEN71_17540 QEN71_32940
PLAD : PPGU16_50900(prpD) PPGU16_65310
CABA : SBC2_55640(prpD) SBC2_62760(prpD2)
BUO : BRPE64_BCDS03620 BRPE64_BCDS13030
BHZ : ACR54_02072(prpD_1) ACR54_03243(prpD_2)
BTRM : SAMEA390648702638(prpD)
BBRO : BAU06_15405 BAU06_18355
BFZ : BAU07_07930 BAU07_11015 BAU07_13510
BOH : AKI39_14785 AKI39_17575(prpD)
BOJ : CBF45_01865 CBF45_13615
BGV : CAL12_16220 CAL12_19295
AXX : ERS451415_01523(prpD)
PUD : G9Q38_01250 G9Q38_13020
POLL : OEG81_12225 OEG81_17090
AMIM : MIM_c13190(prpD) MIM_c20200 MIM_c20300
PIG : EGT29_18235 EGT29_19650 EGT29_21440 EGT29_22655
KGY : EHF36_02240 EHF36_13625
PARK : LSG25_09115 LSG25_17725
URU : DSM104443_01832(prpD_1)
UPL : DSM104440_01699(prpD)
BRAD : BF49_4558 BF49_4697
BRAZ : LRP30_04560 LRP30_41160
STAR : G3545_07575 G3545_12765
RHZ : RHPLAN_07020 RHPLAN_10590 RHPLAN_18500 RHPLAN_30060
DEVO : H4N61_00835 H4N61_16375
SALO : EF888_08285 EF888_14975
RAP : RHOA_0523 RHOA_1130(prpD)
SVC : STVA_15320 STVA_21620(prpD_1)
HSH : NHP194022_12930(prpD)
CAMZ : CVIC12175_1098(prpD)
BAZ : BAMTA208_12370(prpD)
BAE : BATR1942_10370(prpD)
BSON : S101395_00291(prpD)
BTHR : YBT1520_12605(prpD)
BTHU : YBT1518_12685(prpD)
LAO : AOX59_09465(prpD) AOX59_17845
LEX : Len3610_01300 Len3610_10855
AFX : JZ786_19740 JZ786_23330
MTUH : I917_07990 I917_07995
MCHI : AN480_01540 AN480_26290
MMAN : MMAN_38870 MMAN_45570
MSER : MTY59_14180 MTY59_21860
MSHG : MSG_00200 MSG_03882(prpD)
MFJ : MFLOJ_33150 MFLOJ_47750
MSTO : MSTO_37440 MSTO_51750(prpD)
MSIM : MSIM_22680 MSIM_37700(prpD)
MSAK : MSAS_21790 MSAS_35810
MKU : I2456_01535 I2456_20440
MLW : MJO58_01175 MJO58_21280
MCOO : MCOO_00240 MCOO_19970
MBAI : MB901379_01273(prpD)
MSEO : MSEO_26710 MSEO_41030
MMAM : K3U93_00995 K3U93_23655
MPAA : MKK62_05900 MKK62_24955
MWU : PT015_01735 PT015_11575
MYW : BVC93_00950 BVC93_22040
MPHL : MPHLCCUG_00552(prpD)
MTHN : 4412656_00838(prpD)
MMAG : MMAD_18180(prpD) MMAD_28710
MTY : MTOK_57600 MTOK_58360
MSAR : MSAR_02860 MSAR_05270
MAUB : MAUB_17800 MAUB_27520
MSAL : DSM43276_04448(prpD)
MTER : 4434518_01064(prpD)
CGL : Cgl0657(Cgl0657) Cgl0694(Cgl0694) Cgl2538(Cgl2538)
CGB : cg0759(prpD2) cg0796(prpD1) cg2796
CGU : WA5_0628 WA5_0664(PrpD1) WA5_2450
CGS : C624_04080 C624_04290
CGG : C629_04080 C629_04290
CGM : cgp_0759(prpD2) cgp_0796(prpD1) cgp_2796
CGQ : CGLAR1_03735 CGLAR1_03920
CGX : SB89_03485 SB89_03665
CHN : A605_00060 A605_03255
CCG : CCASEI_03480 CCASEI_11970
CGY : CGLY_07715 CGLY_13155(prpD)
CAMG : CAMM_03180 CAMM_10645
CMIN : NCTC10288_02002(prpD)
CPRE : Csp1_12110 Csp1_21770(prpD2)
CSUR : N24_0792 N24_2611(prpD)
CCYS : SAMEA4530656_1891(prpD)
BFV : C628_03730 C628_03915 C628_12560
NFR : ERS450000_04898(prpD)
NNO : NONO_c35960(prpD1) NONO_c62610(prpD2)
NAD : NCTC11293_05413(prpD)
RTM : G4H71_06365 G4H71_17245
TOY : FO059_05720 FO059_17185
MIO : AOA12_07705 AOA12_19260
AMAU : DSM26151_01840(prpD2_1) DSM26151_07950(prpD2_2)
AMAV : GCM10025877_17850 GCM10025877_29120
ALAV : MTO99_12835 MTO99_15675
PSEV : USB125703_00466(prpD2)
ARH : AHiyo8_12570 AHiyo8_12580
AAGI : NCTC2676_1_01044(prpD)
AAU : AAur_1662 AAur_pTC20152
GCR : GcLGCM259_0956(prpD)
KVR : CIB50_0001571(prpD2)
AUL : DCC27_007465 DCC27_010530
AIG : QDX25_07390 QDX25_10510
BRZ : CFK38_11790 CFK38_13510
BKI : M4486_07640 M4486_09140
BHH : Bra3105_04345 Bra3105_05915
PSEI : GCE65_01630 GCE65_14015
ABAM : B1s21122_04870(prpD)
KBS : EPA93_16965 EPA93_35560
FGI : OP10G_1650 OP10G_1760
LCRE : Pla8534_51700(prpD)
CBAM : PIECOFPK_01404(prpD)
SMIZ : 4412673_02507(prpD)
SDJ : NCTC13534_01528(prpD)
STHA : NCTC11429_04834(prpD)
CHRJ : CHRYMOREF3P_4846(prpD)
SRUB : C2R22_03225 C2R22_03240 C2R22_03275 C2R22_03340 C2R22_24245 C2R22_24345
TVO : TVG0362515(TVG0362515)
» show all
Taxonomy
Reference
1
Authors
Aoki H, Tabuchi T.
Title
Purification and properties of 2-methylcitrate dehydratase from Yarrowia lipolytica.
Journal
Agric Biol Chem 45:2831-2837 (1981)
Reference
Authors
Brock M, Maerker C, Schutz A, Volker U, Buckel W
Title
Oxidation of propionate to pyruvate in Escherichia coli. Involvement of methylcitrate dehydratase and aconitase.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 4.2.1.79
ExPASy - ENZYME nomenclature database: 4.2.1.79
LinkDB
All DBs