KEGG ENZYME: 4.2.2.21

ENZYME: 4.2.2.21

Entry

EC 4.2.2.21                 Enzyme

Name

chondroitin-sulfate-ABC exolyase;
chondroitinase (ambiguous);
chondroitin ABC eliminase (ambiguous);
chondroitinase ABC (ambiguous);
chondroitin ABC lyase (ambiguous);
chondroitin sulfate ABC lyase (ambiguous);
ChS ABC lyase (ambiguous);
chondroitin sulfate ABC exoeliminase;
chondroitin sulfate ABC exolyase;
ChS ABC lyase II

Class

Lyases;
Carbon-oxygen lyases;
Acting on polysaccharides

Sysname

chondroitin-sulfate-ABC exolyase

Reaction(IUBMB)

Exolytic removal of Delta4-unsaturated disaccharide residues from the non-reducing ends of both polymeric chondroitin/dermatan sulfates and their oligosaccharide fragments.

Reaction(KEGG)

(other) R10870 R10871 R10872

Comment

This enzyme degrades a variety of glycosaminoglycans of the chondroitin-sulfate- and dermatan-sulfate type. Chondroitin sulfate, chondroitin-sulfate proteoglycan and dermatan sulfate are the best substrates but the enzyme can also act on hyaluronan at a much lower rate. Keratan sulfate, heparan sulfate and heparin are not substrates. The related enzyme EC 4.2.2.20, chondroitin-sulfate-ABC endolyase, has the same substrate specificity but produces a mixture of oligosaccharides of different sizes that are ultimately degraded to tetra- and disaccharides [4]. Both enzymes act by the removal of a relatively acidic C-5 proton of the uronic acid followed by the elimination of a 4-linked hexosamine, resulting in the formation of an unsaturated C4---C5 bond on the hexuronic acid moiety of the products [4,6].

History

EC 4.2.2.21 created 2006 (EC 4.2.2.4 created 1972, part-incorporated 2006 (EC 4.2.99.6 created 1965, part incorporated 1976)), modified 2010

Orthology

K08961

chondroitin-sulfate-ABC endolyase/exolyase

Genes

SES:	SARI_02380

YPE:

YPO0824

YPK:

y3211

YPH:

YPC_0996

YPA:

YPA_0445

YPN:

YPN_3027

YPM:

YP_2832

YPP:	YPDSF_0688

YPG:	YpAngola_A3277

YPZ:

YPZ3_0887

YPT:	A1122_00920

YPD:

YPD4_0844

YPX:

YPD8_0842

YPW:

CH59_1039

YPJ:	CH55_3716 CH55_3717

YPV:

BZ15_2750

YPL:

CH46_92

YPS:

YPTB3073

YPO:

BZ17_3545

YPI:	YpsIP31758_0946

YPY:

YPK_0997

YPB:

YPTS_3194

YPQ:

DJ40_3406

YPU:

BZ21_2390

YPR:

BZ20_3090

YPC:

BZ23_2669

YPF:

BZ19_2453

YSI:	BF17_01850

YRU:

BD65_2433

YRB:	UGYR_12535

PMR:	PMI2127 PMI2128

PMIB:

BB2000_2202 BB2000_2203

PVL:	AOB99_12450 AOB99_12455

PVG:	CRN77_08480 CRN77_08485

PHAU:

PH4a_02555 PH4a_02560

PROT:

BTA34_12080 BTA34_12085

PCOL:

F1325_14000 F1325_14005

PCIB:

F9282_14105 F9282_14110

PPEE:

I6G31_14140 I6G31_14145

PAPP:

QQS39_14100 QQS39_14105

MWI:	MNY66_11575 MNY66_11580

EIC:	NT01EI_3294 NT01EI_3295

ETD:	ETAF_2650 ETAF_2651

ETE:	ETEE_1143 ETEE_1144

ETR:	ETAE_2917 ETAE_2918

ETC:	ETAC_14050 ETAC_14055

EDW:	QY76_05730 QY76_05735

EDL:	AAZ33_15510 AAZ33_15515

EHO:	A9798_02620 A9798_02625

APAG:

EIA51_01230 EIA51_07155

AVT:	NCTC3438_00390 NCTC3438_01189

VAF:	D1115_15465 D1115_15565 D1115_15575

PPR:

PBPRB0153

PGB:	H744_2c1761 H744_2c1762 H744_2c1785 H744_2c1789

PDS:	CAY62_18680

PLZ:	S4054249_13715

ENW:	MJO57_23830

OLE:	K0B96_16470

PUO:	RZN69_09950 RZN69_13390

AGL:

PYTT_0964

LAMB:

KBB96_08400

PBAS:

SMSP2_01417(chonabc_1) SMSP2_01425(chonabc_2)

TAER:

GT409_06540 GT409_06585 GT409_07600 GT409_07615 GT409_07765 GT409_11920 GT409_11925

LPRO:

PQO03_05560 PQO03_05725 PQO03_08425 PQO03_18540

BTH:	BT_3324 BT_3350

BTHO:

Btheta7330_04950(chonabc_1) Btheta7330_04976(chonabc_2)

BHL:	Bache_0583

BXY:	BXY_25910 BXY_26470

BOA:	Bovatus_03443(chonabc_1) Bovatus_03480(chonabc_2)

BCEL:

BcellWH2_02521 BcellWH2_02528(chonabc)

BCAC:

CGC64_04155 CGC64_04295

BCAE:

A4V03_18910 A4V03_19125

BZG:	C4H11_08555

BHF:	C3V43_09815

BIS:	DXK01_003740 DXK01_003755

BUN:	Bun01g_09420(chonabc)

BEG:	INE88_01708 INE88_01710(chonabc)

BSTE:

K6V31_10320 K6V31_11145

BFE:	K6V30_05210 K6V30_05360 K6V30_11685

BNO:	K8P02_06335 K8P02_06350 K8P02_19060 K8P02_19100 K8P02_19165

BHUM:

JXR92_004870

BFC:	BacF7301_06000 BacF7301_06155

BSAL:

K6V25_04780 K6V25_04830

BFIN:

BFINE_06830 BFINE_07270(chonabc)

BACE:

H1A11_16345

COPR:

Cop2CBH44_21620(chonabc_1) Cop2CBH44_21690 Cop2CBH44_25750 Cop2CBH44_27640(chonabc_2)

CFAS:

Cfast33896_15040 Cfast33896_15070 Cfast33896_15120

PDT:	Prede_1246

POC:	NCTC13071_00480

ASH:	AL1_08200 AL1_08220

HLAM:

K4L44_01835 K4L44_01840 K4L44_01850 K4L44_03495 K4L44_12235

HXY:	K5X82_00945 K5X82_01615 K5X82_02295 K5X82_05470 K5X82_13635 K5X82_16070 K5X82_16075 K5X82_16085

COY:	HF329_09485

NSO:	NIASO_15730

NIA:	A8C56_05425

ARK:	D6B99_04970

PHE:	Phep_0784 Phep_2838

PEP:	AQ505_08345

PEJ:	FYC62_06795 FYC62_06820

PGJ:	QG516_06555

PSN:	Pedsa_0057 Pedsa_3805

EST:	DN752_00855

RCG:	N7E81_09370 N7E81_17080

FCO:	FCOL_05500

FGL:	EM308_03130 EM308_03135 EM308_14410

FCM:	BIW12_00075 BIW12_15965 BIW12_15970

FCV:	AWN65_08505

FOE:	JJC03_10185

ZLA:	Q5W13_18245

PHAL:

H9I45_07800

AHZ:	APS56_04620 APS56_04640

FOP:	FNB79_14950

FAY:	Q4Q34_15690

HWS:	RNZ46_12845

ORH:	Ornrh_0305 Ornrh_0807

ORI:	Q785_01660 Q785_04595

CCAU:

EG346_19755 EG346_21420

VHL:	BME96_15890

PKP:	SK3146_03427(chonabc)

LBW:	C3V36_09930

VGU:	HYG85_13675 HYG85_20055

VPY:	HZI73_07975

SBY:	H7H31_29120

STRZ:

OYE22_28345

SGLY:

P8A20_05095

MCHN:

HCR76_14735

ARM:

ART_3703

BRV:	CFK39_06120

BRZ:	CFK38_04965 CFK38_06655

PRV:	G7070_09475

ACIR:

OG979_28660

SRO:

Sros_2256

FMO:	C4N19_04665

CSOM:

MKD34_01195

» show all

Reference

1 [PMID:5647268]

Authors

Yamagata T, Saito H, Habuchi O, Suzuki S.

Title

Purification and properties of bacterial chondroitinases and chondrosulfatases.

Journal

J Biol Chem 243:1523-35 (1968)

Reference

2 [PMID:4231029]

Authors

Saito H, Yamagata T, Suzuki S.

Title

Enzymatic methods for the determination of small quantities of isomeric chondroitin sulfates.

Journal

J Biol Chem 243:1536-42 (1968)

Reference

3 [PMID:5647269]

Authors

Suzuki S, Saito H, Yamagata T, Anno K, Seno N, Kawai Y, Furuhashi T.

Title

Formation of three types of disulfated disaccharides from chondroitin sulfates by chondroitinase digestion.

Journal

J Biol Chem 243:1543-50 (1968)

Reference

4 [PMID:9083041]

Authors

Hamai A, Hashimoto N, Mochizuki H, Kato F, Makiguchi Y, Horie K, Suzuki S

Title

Two distinct chondroitin sulfate ABC lyases. An endoeliminase yielding tetrasaccharides and an exoeliminase preferentially acting on oligosaccharides.

Journal

J Biol Chem 272:9123-30 (1997)
DOI:10.1074/jbc.272.14.9123

Reference

5 [PMID:16336265]

Authors

Huckerby TN, Nieduszynski IA, Giannopoulos M, Weeks SD, Sadler IH, Lauder RM.

Title

Characterization of oligosaccharides from the chondroitin/dermatan sulfates. 1H-NMR and 13C-NMR studies of reduced trisaccharides and hexasaccharides.

Journal

FEBS J 272:6276-86 (2005)
DOI:10.1111/j.1742-4658.2005.05009.x

Reference

6 [PMID:18992215]

Authors

Zhang Z, Park Y, Kemp MM, Zhao W, Im AR, Shaya D, Cygler M, Kim YS, Linhardt RJ

Title

Liquid chromatography-mass spectrometry to study chondroitin lyase action pattern.

Journal

Anal Biochem 385:57-64 (2009)
DOI:10.1016/j.ab.2008.10.014

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