KEGG   ENZYME: 4.2.3.153
Entry
EC 4.2.3.153                Enzyme                                 

Name
(5-formylfuran-3-yl)methyl phosphate synthase;
mfnB (gene name);
4-HFC-P synthase;
4-(hydroxymethyl)-2-furaldehyde phosphate synthase
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
Sysname
D-glyceraldehyde-3-phosphate phosphate-lyase [D-glyceraldehyde-3-phosphate-adding; (5-formylfuran-3-yl)methyl-phosphate-forming]
Reaction(IUBMB)
2 D-glyceraldehyde 3-phosphate = (5-formylfuran-3-yl)methyl phosphate + phosphate + 2 H2O [RN:R10935]
Reaction(KEGG)
R10935
Substrate
D-glyceraldehyde 3-phosphate [CPD:C00118]
Product
(5-formylfuran-3-yl)methyl phosphate [CPD:C20954];
phosphate [CPD:C00009];
H2O [CPD:C00001]
Comment
The enzyme catalyses the reaction in the direction of producing (5-formylfuran-3-yl)methyl phosphate, an intermediate in the biosynthesis of methanofuran. The sequence of events starts with the removal of a phosphate group, followed by aldol condensation and cyclization. Methanofuran is a carbon-carrier cofactor involved in the first step of the methanogenic reduction of carbon dioxide by methanogenic archaea.
History
EC 4.2.3.153 created 2015 as EC 4.1.99.21, transferred 2015 to EC 4.2.3.153
Pathway
ec00680  Methane metabolism
ec01100  Metabolic pathways
Orthology
K09733  (5-formylfuran-3-yl)methyl phosphate synthase
Genes
MCA: MCA1243
METU: GNH96_09840
MMT: Metme_2177
MDN: JT25_017785
MDH: AYM39_09595
MKO: MKLM6_2030
METL: U737_08655
MPAD: KEF85_08895
MAH: MEALZ_1631
MBUR: EQU24_12260
MMAI: sS8_3940
MEJ: Q7A_320
MEC: Q7C_2000
NOC: Noc_0020
NHL: Nhal_0008
NWA: Nwat_0015
NTT: TAO_0004
CVI: CV_3961
MPT: Mpe_A2618
LCH: Lcho_3141
RGE: RGE_40330
METR: BSY238_70
MFA: Mfla_1579
MMB: Mmol_0899
MEH: M301_0953
MEP: MPQ_1682
MPAU: ZMTM_14810
XAU: Xaut_4098
SNO: Snov_0761
MDI: METDI2528
MEX: Mext_1844
MCH: Mchl_2179
MPO: Mpop_1795
MET: M446_5015
MNO: Mnod_5319
MOR: MOC_3090
META: Y590_08835
MIND: mvi_45740
MSL: Msil_2396
HDN: Hden_1466
MCG: GL4_1772
MSC: BN69_1044
HDI: HDIA_2298
SCO: SCO6440(SC9B5.07)
SGR: SGR_6403
SGB: WQO_03045
SHY: SHJG_8696
SMAL: SMALA_7956
SFA: Sfla_6110
SBH: SBI_00630
STRP: F750_0451
SFI: SFUL_24
SALU: DC74_350
SALL: SAZ_02200
SRW: TUE45_pSRc_0556(TUE45_pSRTUE45c_0556)
STRD: NI25_06195
SRJ: SRO_7133
CAI: Caci_2711
RBA: RB5685
PSL: Psta_3306
RUL: UC8_03640
AHEL: Q31a_45930
PLM: Plim_1240
FTJ: FTUN_3137
IPA: Isop_2173
SACI: Sinac_2321
MOX: DAMO_2674
MJA: MJ_1099
MMP: MMP0708
MMD: GYY_02715
MMAD: MMJJ_02690
MAE: Maeo_0482
MVO: Mvol_0288
MTH: MTH_141
METC: MTCT_0106
MWO: MWSIV6_0556(mfnB)
METE: tca_00125
MST: Msp_0430
MRU: mru_1209
MSI: Msm_1628
MEB: Abm4_0760
MMIL: sm9_1400
MEYE: TL18_04040
MOL: YLM1_0623
METH: MBMB1_0925
MFC: BRM9_1097
MCUB: MCBB_1210(mfnB)
MFV: Mfer_0195
MKA: MK1602
AFU: AF_2196
FPL: Ferp_0372
GAC: GACE_1910
GAH: GAH_00745
MBAR: MSBR2_3309
MBAK: MSBR3_3310
MAC: MA_4436
MMA: MM_1114
MMAC: MSMAC_0194
METM: MSMTP_0219
MTHR: MSTHT_1717
MTHE: MSTHC_1571
MHOR: MSHOH_0241
MBU: Mbur_1676
MMET: MCMEM_2157
MMH: Mmah_1977
MPY: Mpsy_1294
MTP: Mthe_0348
MCJ: MCON_2254
MHI: Mhar_0838
MHU: Mhun_2631
MLA: Mlab_1383
MEMA: MMAB1_1009
MPI: Mpet_2359
MBN: Mboo_1717
MPL: Mpal_2061
MPD: MCP_2988
MEZ: Mtc_0569
RCI: RCIX681
BARB: AOA66_0928
 » show all
Reference
1  [PMID:24977328]
  Authors
Miller D, Wang Y, Xu H, Harich K, White RH
  Title
Biosynthesis of the 5-(Aminomethyl)-3-furanmethanol moiety of methanofuran.
  Journal
Biochemistry 53:4635-47 (2014)
DOI:10.1021/bi500615p
  Sequence
[mja:MJ_1099]
Reference
2  [PMID:25372812]
  Authors
Bobik TA, Morales EJ, Shin A, Cascio D, Sawaya MR, Arbing M, Yeates TO, Rasche ME
  Title
Structure of the methanofuran/methanopterin-biosynthetic enzyme MJ1099 from Methanocaldococcus jannaschii.
  Journal
Acta Crystallogr F Struct Biol Commun 70:1472-9 (2014)
DOI:10.1107/S2053230X1402130X
  Sequence
[mja:MJ_1099]
Reference
3  [PMID:25905665]
  Authors
Wang Y, Jones MK, Xu H, Ray WK, White RH
  Title
Mechanism of the Enzymatic Synthesis of 4-(Hydroxymethyl)-2- furancarboxaldehyde-phosphate (4-HFC-P) from Glyceraldehyde-3-phosphate Catalyzed by 4-HFC-P Synthase.
  Journal
Biochemistry 54:2997-3008 (2015)
DOI:10.1021/acs.biochem.5b00176
  Sequence
[mja:MJ_1099]
Other DBs
ExplorEnz - The Enzyme Database: 4.2.3.153
IUBMB Enzyme Nomenclature: 4.2.3.153
ExPASy - ENZYME nomenclature database: 4.2.3.153
BRENDA, the Enzyme Database: 4.2.3.153
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