KEGG   ENZYME: 4.4.1.22
Entry
EC 4.4.1.22                 Enzyme                                 
Name
S-(hydroxymethyl)glutathione synthase;
glutathione-dependent formaldehyde-activating enzyme;
Gfa;
S-(hydroxymethyl)glutathione formaldehyde-lyase
Class
Lyases;
Carbon-sulfur lyases;
Carbon-sulfur lyases (only sub-subclass identified to date)
Sysname
S-(hydroxymethyl)glutathione formaldehyde-lyase (glutathione-forming)
Reaction(IUBMB)
S-(hydroxymethyl)glutathione = glutathione + formaldehyde [RN:R06982]
Reaction(KEGG)
R06982
Substrate
S-(hydroxymethyl)glutathione [CPD:C14180]
Product
glutathione [CPD:C00051];
formaldehyde [CPD:C00067]
Comment
The enzyme from Paracoccus denitrificans accelerates the spontaneous reaction in which the adduct of formaldehyde and glutathione is formed, i.e. the substrate for EC 1.1.1.284, S-(hydroxymethyl)glutathione dehydrogenase, in the formaldehyde-detoxification pathway.
History
EC 4.4.1.22 created 2005 (EC 1.2.1.1 created 1961, modified 1982, modified 2002, part transferred 2005 to EC 4.4.1.22)
Pathway
ec00680  Methane metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K03396  S-(hydroxymethyl)glutathione synthase
Genes
MPHA: 105839794
SMP: SMAC_08712
PAN: PODANSg505
TTT: THITE_49799
TMN: UCRPA7_692
SSCK: SPSK_01984
FGR: FGSG_10776
FPU: FPSE_08822
FVR: FVEG_11444
FOX: FOXG_12705
NHE: NECHADRAFT_69107
MAJ: MAA_09901
PLJ: VFPFJ_02045
VAL: VDBG_01395
VDA: VDAG_07855
CFJ: CFIO01_10786
CHIG: CH63R_05407
ELA: UCREL1_2225
PFY: PFICI_05448
PSCO: LY89DRAFT_687122
ANG: ANI_1_14024(An02g00080)
AFV: AFLA_005034
ALUC: AKAW2_21597S
ACHE: ACHE_21301A
PCS: Pc20g15670
PDP: PDIP_47330
TMF: PMAA_051990
TRG: TRUGW13939_01253
PNO: SNOG_01600
PTE: PTT_17259
BZE: COCCADRAFT_106556
BSC: COCSADRAFT_36926
BOR: COCMIDRAFT_86974
AALT: CC77DRAFT_1024765
ZTR: MYCGRDRAFT_87443
PFJ: MYCFIDRAFT_87783
FFU: CLAFUR5_11225
ADP: NCTC12871_00268(gfa)
XCC: XCC3388
XCB: XC_0776
XCA: xcc-b100_0811(gfa)
XCP: XCR_3724(gfa)
XCV: XCV0789
XAX: XACM_0733
XAC: XAC0735
XCI: XCAW_03847
XCT: J151_00858
XCJ: J158_00855
XCU: J159_00855
XCN: J169_00854
XCW: J162_00854
XCR: J163_00854
XCM: J164_00854
XCF: J172_00849
XFU: XFF4834R_chr07430
XAO: XAC29_03740
XOM: XOO3646(XOO3646)
XOP: PXO_04414
XOZ: BE73_20410
XSA: SB85_16995
XTN: FD63_15635
XVE: BJD12_08565
XPE: BJD13_13045
XHR: XJ27_19785
XPH: XppCFBP6546_16605(gfa)
XVA: C7V42_18175(gfa)
XAN: AC801_17780
XAR: XB05_12165
XHY: FZ025_12510(gfa)
XTH: G4Q83_19135(gfa)
XEU: XSP_000761(gfa)
XHD: LMG31886_08570(gfa)
LARE: HIV01_015560(gfa)
DTX: ATSB10_32700
DKO: I596_2122
VFI: VF_A0007
VFM: VFMJ11_A0021(gfa)
VSA: VSAL_II0213(gfa)
PPR: PBPRB1570
PDS: CAY62_04965
PCQ: PcP3B5_24880(gfa)
PPX: T1E_5519
PSB: Psyr_2957
PPUU: PputUW4_02203(gfa)
PKC: PKB_4293(gfa)
PFK: PFAS1_28165
PSIL: PMA3_14295
PAZE: KSS91_12045(gfa)
PCAV: D3880_11230
ATN: FM020_06240(gfa)
SFR: Sfri_3436 Sfri_3438
SSE: Ssed_4230
SPL: Spea_0267 Spea_3387
SHL: Shal_3474 Shal_4035
SWD: Swoo_0373
SVO: SVI_3944(gfa)
SEUR: FM038_023190(gfa)
PSY: PCNPT3_05390
MVS: MVIS_3174(gfa)
MMAA: FR932_02530(gfa)
HHU: AR456_12705
HALO: BWR19_16725
HCAM: I4484_18760(gfa)
HSX: HNO51_18715(gfa)
HSV: HNO53_19035(gfa)
HTX: EKK97_20970(gfa)
SALN: SALB1_1489
BAM: Bamb_5797
BAC: BamMC406_5579
BPY: Bphyt_4238 Bphyt_5113
PACP: FAZ97_30990(gfa)
AKA: TKWG_16070
AMIM: MIM_c14300(gfa)
PARK: LSG25_15680(gfa)
PVAC: HC248_01255(gfa)
VEI: Veis_4024
MMS: mma_2819
MALI: EYF70_29695(gfa)
MUM: FCL38_19970(gfa)
MFLA: GO485_25000(gfa)
MPLI: E1742_23860(gfa)
URU: DSM104443_01527(gfa)
BEBA: BWI17_19425
MLO: mlr0874
MLN: A9174_21485
MOP: Mesop_4082
MAM: Mesau_03843
MAMO: A6B35_20710 A6B35_25035
MHUA: MCHK_2549(gfa)
MJR: EB229_21270(gfa)
MERD: EB233_18455(gfa)
MESR: FGU64_02285(gfa)
AAK: AA2016_2457
AMIH: CO731_01953(gfa_1) CO731_02450(gfa_2)
SME: SM_b20186
SMK: Sinme_3979
SMQ: SinmeB_4476
SMX: SM11_pD1417
SMI: BN406_06470(gfa)
SMEG: C770_GR4pD1402
SMEL: SM2011_b20186(gfa)
SMER: DU99_32400
SMD: Smed_3930
RHI: NGR_b14630(gfa1) NGR_c19300 NGR_c20490(gfa2)
SFH: SFHH103_01861 SFHH103_05888
SFD: USDA257_c31100(gfa1) USDA257_c43530(gfa2) USDA257_c54270(gfa3)
SAME: SAMCFNEI73_Ch0088(gfa)
SINO: SS05631_c38600
EAD: OV14_a1033(gfa2)
EAH: FA04_27895 FA04_30330
ESJ: SJ05684_b47720 SJ05684_b58890
EMX: FKV68_00780(gfa) FKV68_13155(gfa) FKV68_33100(gfa)
RET: RHE_PF00400
REC: RHECIAT_PC0000582
REL: REMIM1_PF00508(gfa)
REP: IE4803_PC00392(gfa)
REI: IE4771_PD00410(gfa)
RLE: pRL120526(gfA)
RLT: Rleg2_4920 Rleg2_4958
RLG: Rleg_4832
RLB: RLEG3_07960
RLU: RLEG12_10940
RHL: LPU83_2360(gfa)
RGA: RGR602_PC00929(gfa-1) RGR602_PC02286(gfa-2)
RHN: AMJ98_PE00477(gfa)
RPHA: AMC79_PD00607(gfa)
RHX: AMK02_PD00340(gfa)
RHK: Kim5_PC00370(gfa)
REZ: AMJ99_PD00477(gfa)
RJG: CCGE525_10885(gfa)
RGR: FZ934_22410(gfa)
RAD: CO657_27365(gfa)
RII: FFM53_027555(gfa)
RHID: FFM81_024520(gfa)
RBQ: J2J99_23700(gfa)
RLN: J0663_23815(gfa)
RBAN: J2J98_23015(gfa)
RRG: J3P73_26630(gfa)
RHT: NT26_3258(gfa)
RFV: RFYW14_03122
SHZ: shn_31780
SZO: K8M09_22425(gfa)
KAI: K32_22190(gfa)
BJA: blr6216(blr6216)
BJU: BJ6T_34850
BJP: RN69_16905
BRA: BRADO5487(gfa)
BBT: BBta_5971(gfa)
BRS: S23_20790
AOL: S58_22210
BRC: BCCGELA001_26715
BRAD: BF49_5354 BF49_5366
BIC: LMTR13_13680
BRO: BRAD285_1746(gfa)
BRK: CWS35_16195(gfa) CWS35_32580(gfa)
BOT: CIT37_23150(gfa)
BRQ: CIT40_23255(gfa)
BGQ: X265_11175(gfa)
BGZ: XH91_10400(gfa)
BSYM: CIT39_22250(gfa)
BBET: F8237_25275(gfa)
BARH: WN72_34695(gfa)
BVZ: BRAD3257_2931(gfa)
BEL: BE61_38370
BDG: LPJ38_32960(gfa)
BSEP: HAP48_0048215(gfa)
BQB: J4P68_0007425(gfa)
RPC: RPC_0100
RPE: RPE_4679
TRB: HB776_18780(gfa)
MOC: BB934_29595
BBAR: RHAL1_00658(gfa) RHAL1_01784
BRN: D1F64_20380(gfa)
NOH: G5V57_12155(gfa)
ACUT: MRB58_02640(gfa)
YPAC: CEW88_21860(gfa)
YAN: AYJ57_23665
TPRO: Ga0080559_TMP1801
PABY: Ga0080574_TMP304
SAGU: CDO87_25880(gfa)
OCD: FHY55_11710(gfa)
MALU: KU6B_56540(gfa)
PALX: GQA70_00385(gfa)
RSP: RSP_2575
RSH: Rsph17029_1233
RSQ: Rsph17025_1948
RSK: RSKD131_0887
CAZT: LV780_08635(gfa)
PDE: Pden_0015
PAMI: JCM7686_0085
PYE: A6J80_09895(gfa)
PCON: B0A89_05655
PZH: CX676_13675(gfa)
PARO: CUV01_07510(gfa)
PAMN: JCM7685_0202(gfa)
PMUT: DPM13_08095(gfa)
PARS: DRW48_11880(gfa)
PARR: EOJ32_02150(gfa)
PKD: F8A10_00210(gfa)
PPAN: ESD82_21255(gfa)
PLIA: E4191_03490(gfa) E4191_06110(gfa) E4191_14885(gfa)
PSAN: HGN31_08085(gfa)
PMAU: CP157_00050(gfa)
DAA: AKL17_0665
GFU: KM031_18915(gfa)
HML: HmaOT1_09240(gfa)
PPRU: FDP22_18560(gfa)
PAMO: BAR1_01170(gfa)
PGV: SL003B_0765
SPHA: D3Y57_02580(gfa)
ACR: Acry_2793
AMV: ACMV_31250(gfa)
RMT: IAI58_19635(gfa)
SWF: E3E12_02505(gfa)
AZZ: DEW08_23520(gfa)
SKT: IGS68_32150(gfa)
SMUC: JL100_034250(gfa)
SROE: JL101_028910(gfa)
HTQ: FRZ44_12310(gfa)
HADH: FRZ61_12390(gfa)
MAGQ: MGMAQ_0499(gfa)
AFR: AFE_0696(gfa)
AFE: Lferr_0844
AFI: Acife_0709
AFJ: AFERRID_19300
PCX: LPB68_21510
SIV: SSIL_2807
PLL: I858_008940
PHC: BBI08_11300
CRN: CAR_c23670(gfa)
CAW: Q783_10630
CVD: LHA31_07905(gfa)
 » show all
Reference
1  [PMID:11741920]
  Authors
Goenrich M, Bartoschek S, Hagemeier CH, Griesinger C, Vorholt JA
  Title
A glutathione-dependent formaldehyde-activating enzyme (Gfa) from Paracoccus denitrificans detected and purified via two-dimensional proton exchange NMR spectroscopy.
  Journal
J Biol Chem 277:3069-72 (2002)
DOI:10.1074/jbc.C100579200
  Sequence
[pde:Pden_0015]
Other DBs
ExplorEnz - The Enzyme Database: 4.4.1.22
IUBMB Enzyme Nomenclature: 4.4.1.22
ExPASy - ENZYME nomenclature database: 4.4.1.22
UM-BBD (Biocatalysis/Biodegradation Database): 4.4.1.22
BRENDA, the Enzyme Database: 4.4.1.22
CAS: 425642-27-9
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