KEGG   ENZYME: 4.4.1.25
Entry
EC 4.4.1.25                 Enzyme                                 
Name
L-cysteate sulfo-lyase;
L-cysteate sulfo-lyase (deaminating);
CuyA;
L-cysteate bisulfite-lyase (deaminating;
pyruvate-forming)
Class
Lyases;
Carbon-sulfur lyases;
Carbon-sulfur lyases (only sub-subclass identified to date)
Sysname
L-cysteate hydrogensulfite-lyase (deaminating; pyruvate-forming)
Reaction(IUBMB)
L-cysteate + H2O = hydrogensulfite + pyruvate + NH3 (overall reaction) [RN:R07634];
(1a) L-cysteate = hydrogensulfite + 2-aminoprop-2-enoate;
(1b) 2-aminoprop-2-enoate = 2-iminopropanoate (spontaneous);
(1c) 2-iminopropanoate + H2O = pyruvate + NH3 (spontaneous)
Reaction(KEGG)
R07634
Substrate
L-cysteate [CPD:C00506];
H2O [CPD:C00001];
2-aminoprop-2-enoate [CPD:C02218];
2-iminopropanoate [CPD:C20904]
Product
hydrogensulfite;
pyruvate [CPD:C00022];
NH3 [CPD:C00014];
2-aminoprop-2-enoate [CPD:C02218];
2-iminopropanoate [CPD:C20904]
Comment
A pyridoxal-phosphate protein. The enzyme cleaves a carbon-sulfur bond, releasing hydrogensulfite and an unstable enamine product that tautomerizes to an imine form, which undergoes a hydrolytic deamination to form pyruvate and ammonia. The latter reaction, which can occur spontaneously, can also be catalysed by EC 3.5.99.10, 2-iminobutanoate/2-iminopropanoate deaminase. D-Cysteine can also act as a substrate, but more slowly. It is converted into hydrogen sulfide, pyruvate, and ammonia. This inducible enzyme from the marine bacterium Silicibacter pomeroyi DSS-3 forms part of the cysteate-degradation pathway.
History
EC 4.4.1.25 created 2006
Pathway
ec00270  Cysteine and methionine metabolism
ec01100  Metabolic pathways
Orthology
K17950  L-cysteate sulfo-lyase
Genes
ENFAKI40_4522
EHUD5067_0020985
EMORL6Y89_01925
KOXKOX_18740
KOEA225_2619
KOYJ415_18880
KOMHR38_17105
KOKKONIH1_12530
RORRORB6_16680
RAODSD31_23505
RTGNCTC13098_02583(dcyD_1)
REEelectrica_04627(cuyA)
CKOCKO_02077
CAMAF384_24035
CFARCI104_20845
CTELGBC03_08970
KORAWR26_22860
KRDA3780_00880
KASKATP_41030
METYMRY16398_50810
KINAB182_22850
PDZHHA33_25565
PSGCG163CM_17450(cuyA)
EBFD782_2775
EBCC2U52_04905
SFOZ042_18480
RAHRahaq_2317
RACEJHW33_05260
RAQRahaq2_2422
RAAQ7S_11730
ROXBV494_05270
RVCJ9880_18790
GQUAWC35_03525
EAMEGXP68_22095
ECAECA1531
PATREV46_07745
PATOGZ59_15690
PCTPC1_1404
PCVBCS7_07070
PWAPecwa_1802
PPARA8F97_09765
PECW5S_1716
PWSA7983_22495
PPOABJK05_02255
PARII2D83_14975
PVZOA04_28770
DDDDda3937_02691(dcyD)
DSOA4U42_16645
CEDLH89_04525
DFNCVE23_08235
DDQDDI_1238
DICDpoa569_002382
DDADd703_1185 Dd703_1301
BGJAWC36_10070 AWC36_10530
BRBEH207_11330
BNGEH206_10630 EH206_14450
BIZHC231_14730 HC231_15080
LBQCKQ53_11485
SLIGGTU79_18865
PAOPat9b_5047
PANSFCN45_09535
LRZBJI69_08935
VTUIX91_16745
VKABTD91_14785
PMANOU5_6079
PAZOAYR47_22335
PKRAYO71_16820
PSILPMA3_13030
PUMHGP31_13520
PLALFXN65_13345 FXN65_14445
PATAJWU58_13515
PQIKH389_23850
PFAKKSS94_12650
PWYHU734_019335
PARPsyc_1604
PCRPcryo_1828
PSOPSYCG_09725
PURAOC03_09170
PSPGAK823_04080
PSYGAK825_04005
ACDAOLE_12720
ALABFG52_04285
AWUBEN71_14650
SPLSpea_4164
SHLShal_0079
SPSWSps_03974
SPSHFM037_08130
SYKKDN34_08480
COLWA3Q33_01580
CBERB5D82_04565
THAPFNC98_09245
PATPatl_2666
GAGGlaag_1613 Glaag_1767
GPSC427_0873 C427_3081
PMESFX988_04207
LALAT746_08525
SALHHMF8227_01275
AGZM0C34_19920
MICCAUP74_01091(cuyA_1)
LFALFA_0645
THIMKFB96_04105
TBOGLT988_01060
SPIUSPICUR_05155
WOCBA177_13350 BA177_17580
HAFC8233_03390
ZPLZBT109_0393
CAMHLCW13_07835
PAURFGL86_17210
MARSA8C75_20710
NJPNEJAP_0138 NEJAP_1593 NEJAP_3675
SALNSALB1_2529
TSNW908_04440
NBACUN60_08590
CCAMM5D45_24730
BURBcep18194_C6869
BDFWI26_24255
BGDbgla_2g09270
BGOBM43_4559
BGPBGL_2c08150
BPLAbpln_2g09040
BPHBphy_5685
BGEBC1002_4820
BUZAYM40_10365
BFNOI25_8251
BCAIK788_0004377
PSPWBJG93_22590
PHSC2L64_41080
PTERC2L65_37970
PMEGFNZ07_07325
PTROG5S35_24805
PACPFAZ97_32470
PACSFAZ98_29685
PEWKZJ38_15460
PSPUNA29_16035
PTXABW99_09980
PNRAT302_11325 AT302_19680
BPARBN117_4146
BPABPP4074
BBHBN112_3877
BBRBB4545
BBMBN115_4222
BBXBBS798_4322
BBROBAU06_13605
BOHAKI39_12935
AXONH44784_051791
AXNAX27061_3316
AXXERS451415_03296(acdS_2)
ADTAPT56_16345
PUTPT7_2783 PT7_3194
BOZDBV39_16635
PARKLSG25_20075
RTARta_27290(dcyD)
CBXCenrod_1469(dcyD)
HYBQ5W_20320
DRGH9K76_10835
JAGGJA_4883
HHTF506_07715
SWSI6J16_07420
DDZDSYM_26300
DATHRM2_15340(dcyD1)
PAUUE8A73_043310
SAMYDB32_000435
PCAYFRD00_28910
MLNA9174_10725
MESOBSQ44_20105
PLAPlav_0934
PMOBHG718_08940
RBSRHODOSMS8_00523(cuyA)
SMDSmed_5532
RLGRleg_6848
RGRFZ934_21710
RADCO657_30285
NENNCHU2750_12260
BARHWN72_37835
BELBE61_69980
BOSBSY19_2081
BOFFQV39_17920
APRAG3A50_13350 G3A50_21605
APOLK9D25_16725
MEAMex_1p2360(dcyD)
MDIMETDI3140(dcyD)
MEXMext_2357
MCHMchl_2636
MPOMpop_2314
MZAB2G69_19395
METAY590_11380
METDC0214_13220
METSDK389_03615
METIDK427_02200
MTADM6G65_30955
MIVC4E04_00385
BBARRHAL1_03100
DEAFPZ08_20155
MMEDMame_04307(cuyA)
AUAM673_00800
LABPFJ695_09125 FJ695_16525
ACUTMRB58_24525
CCRCC_2032
CCSCCNA_02112
CAKCaul_3180
CSECseg_1395
CHQAQ619_11480
CMBCSW64_07525
CFHC1707_25095
CAUFCSW63_10225
PZUPHZ_c2912
PHBHYN04_01540
BSBBresu_3207
BRGA4249_15745
BRLBZG35_15890
BVCCEP68_11000
BRFE4M01_07420
BREVE7T10_15055
BGOEIFJ75_14085
BVITJIP62_06445
SILSPO2657 SPOA0158(cuyA)
RUAD1823_13230 D1823_20180
RUTFIU92_00390(cuyA) FIU92_19550(dcyD)
RDERD1_0819 RD1_1838
RLIRLO149_c007450(cuyA)
DSHDshi_1439
PGAPGA1_c25520(dcyD) PGA1_c33720(dcyD2)
PGDGal_02808
LMDMETH_22250
LAQUR2C4_20010
YANAYJ57_18010
TPROGa0080559_TMP3711
SULZC1J03_10965 C1J03_15755
SULIC1J05_16085
SULDB5M07_10370
SMEDJNX03_20360
SINLDSM14862_01602(cuyA)
RMMROSMUCSMR3_01902(cuyA) ROSMUCSMR3_02142(cuyA)
ROKRAK1035_1044 RAK1035_1283
ROMEI983_02810
ROHFIU89_02605(cuyA)
AHTANTHELSMS3_04525(cuyA)
SAGUCDO87_14615
THAACFI11_07285
MALUKU6B_40360
PAMIJCM7686_pAMI4p082
PYEA6J80_16380
PARSDRW48_00910 DRW48_00915 DRW48_11285
PTPRCA23_c24520(dcyD)
RHCRGUI_2479
THWBMG03_09545
RBGBG454_17005
GEHHYN69_00380
TAWEI545_14695
BOOE2K80_12215
PAEDG5B38_17050
ZMIZCP4_1823
ZMCA265_01765(dcyD)
ZMRA254_01785(dcyD)
ZMPZymop_1074
SPHSETR14_14220
RMTIAI58_10035
RAPRHOA_5757(dcyD)
SHUMSTHU_12380
SVCSTVA_36770
STELSTAQ_37300
APBSAR116_2240
SNEANBZ79_05565
BSHILGQ02_19575
PSABPSAB_12415
PDUPDUR_14060
PBJVN24_13960
PSOPKP014_17645
LFBC1X05_07620
LHILG8J22_02199(cuyA)
LNNF0161_09555
EAVEH197_11010
CNNCNEO_1046
PFAAMM59RIKEN_16910
AFTBBF96_00385
MGROFZ046_14265
MMAGMMAD_31310
MARZMARA_41600
NGPLTT66_36430
RERRER_54610
REYO5Y_26010
REBXU06_26290
RQIC1M55_27535
RHODAOT96_07500
SVLStrvi_4207
SHYSHJG_8020
SHOSHJGH_7782
SRCM271_04440
SMALSMALA_7536
SCIB446_32200
SRWTUE45_00250(dcyD)
SPAVSpa2297_33580
SGSAVL59_17185
SPUNBFF78_04225
SRJSRO_0788
SFYGFH48_34575
SGFHEP81_00770
CCAPAES38_06105
PLAPEAO79_09915
ALXLVQ62_03960
TEHGKE56_14010
NOOFE634_13155
AEZC3E78_06300
MMARMODMU_3995
KRAKrad_0767
AMNRAM_02115
AMMAMES_0412(dcyD)
AMZB737_0413(dcyD)
AOIAORI_0427(dcyD)
AJAAJAP_37515
AMYCCU254_00815 CU254_14920
AMYBBKN51_36300
AORISD37_39110
KALKALB_4130
KPHYAOZ06_19000 AOZ06_21100
ACTNL083_6387
AFSAFR_32880
ATLAthai_62100
ASERAsera_13220
CCAZCOUCH_10905
ABAIIMCC26256_11216
AMRAM1_1636
STISthe_0126
ATMANT_22590
PBFCFX0092_A3104(cuyA)
KBSEPA93_01050
THUGKNN16_03545
LPAVPLANPX_3584
SDYNMal52_16510(cuyA)
GESVT84_20045(cuyA)
GOGC1280_20085
GMSSOIL9_30630
TIMGMBLW1_01380
LRSPX52LOC_00597
FTJFTUN_7586
ULIETAA1_31550(cuyA)
UAMUABAM_04233
ABACLuPra_03549(cuyA_2)
TTZFHG85_02765
SMIZ4412673_02127(dcyD_1)
FBUUJ101_01236(dcyD)
CNKEG343_05330
VPMKG892_02910 KG892_04490
BARCAOA65_2400
 » show all
Reference
1  [PMID:16302849]
  Authors
Denger K, Smits TH, Cook AM
  Title
L-cysteate sulpho-lyase, a widespread pyridoxal 5'-phosphate-coupled desulphonative enzyme purified from Silicibacter pomeroyi DSS-3(T).
  Journal
Biochem J 394:657-64 (2006)
DOI:10.1042/BJ20051311
  Sequence
[sil:SPOA0158]
Other DBs
ExplorEnz - The Enzyme Database: 4.4.1.25
IUBMB Enzyme Nomenclature: 4.4.1.25
ExPASy - ENZYME nomenclature database: 4.4.1.25
BRENDA, the Enzyme Database: 4.4.1.25
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