Isomerases;
Racemases and epimerases;
Acting on amino acids and derivatives
BRITE hierarchy

UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L-glutamate L-glutamate-epimerase

ATP + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L-glutamate + H2O = AMP + diphosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamate [RN:R12271]

R12271
Reaction

ATP [CPD:C00002];
UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L-glutamate [CPD:C22053];
H2O [CPD:C00001]

The enzyme, characterized from the bacterium Xanthomonas oryzae, catalyses epimerization of the terminal L-glutamate in UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-L-glutamate. The reaction proceeds only in one direction and involves an adenylated intermediate. The combined activity of this enzyme and EC 6.3.2.53, UDP-N-acetylmuramoyl-L-alanine---L-glutamate ligase, provides an alternative route for incorporating D-glutamate into peptidoglycan, replacing the more common combination of EC 5.1.1.3, glutamate racemase, and EC 6.3.2.9, UDP-N-acetylmuramoyl-L-alanine---D-glutamate ligase.

EC 5.1.1.23 created 2018

1  [PMID:28294606]