KEGG   ENZYME: 5.1.3.37
Entry
EC 5.1.3.37                 Enzyme                                 
Name
mannuronan 5-epimerase;
algG (gene name);
alginate epimerase;
C5-mannuronan epimerase;
mannuronan C-5-epimerase
Class
Isomerases;
Racemases and epimerases;
Acting on carbohydrates and derivatives
Sysname
[mannuronan]-beta-D-mannuronate 5-epimerase
Reaction(IUBMB)
[mannuronan]-beta-D-mannuronate = [alginate]-alpha-L-guluronate
Reaction(KEGG)
(other) R08693
Substrate
[mannuronan]-beta-D-mannuronate
Product
[alginate]-alpha-L-guluronate
Comment
The enzyme epimerizes the C-5 bond in some beta-D-mannuronate residues in mannuronan, converting them to alpha-L-guluronate residues, and thus modifying the mannuronan into alginate. It is found in brown algae and alginate-producing bacterial species from the Pseudomonas and Azotobacter genera.
History
EC 5.1.3.37 created 2015
Pathway
ec00051  Fructose and mannose metabolism
ec01100  Metabolic pathways
Orthology
K01795  mannuronan 5-epimerase
Genes
PAEPA3545(algG)
PAEVN297_3668(algG)
PAEIN296_3668(algG)
PAUPA14_18500(algG)
PAPPSPA7_1600(algG)
PAGPLES_14881(algG)
PAFPAM18_1441(algG)
PNCNCGM2_4670(algG)
PAEBNCGM1900_2739(algG)
PDKPADK2_06640
PSGG655_07075
PRPM062_18780
PAEPPA1S_07695
PAERPA1R_gp1363
PAEMU769_07170
PAELT223_07410
PAESSCV20265_1518
PAEUBN889_03916(algG)
PAEGAI22_26210
PAECM802_3665(algG)
PAEOM801_3533(algG)
PMYPmen_1026 Pmen_1667
PMKMDS_1077
PREPCA10_11830(algG) PCA10_38180(algG)
PFUWKF707C_11120
PALCA0T30_03860 A0T30_11830
PCQPcP3B5_50730(algG)
PMUIG4G71_08950
PPUPP_1283(algG)
PPFPput_4442
PPGPputGB1_4566
PPWPputW619_0884
PPTPPS_4408
PPBPPUBIRD1_4278
PPIYSA_03103
PPXT1E_3163
PPUHB479_22175
PPUTL483_27260
PPUNPP4_07950(algG)
PPUDDW66_4799
PFVPsefu_3891
PMONX969_21710
PMOTX970_21345
PMOSO165_018585
PPJRK21_00114
PMOLCLJ08_08405 CLJ08_22875
PSTPSPTO_1238(algG)
PSBPsyr_1058
PSYRN018_20380
PSPPSPPH_1113(algG)
PSAVPSA3335_09070
PAMGBKM19_008060
PCIPCH70_10080
PAVLBKM03_06995
PVDCFBP1590__4282(algG)
PCABJGS08_19550(algG)
PCOFPOR16_18950
PTREI9H09_05065(algG)
PSYIMME58_05250(algG)
PASTN015_05960
PFLPFL_1019(algG)
PPRCPFLCHA0_c10390(algG)
PPROPPC_1057
PFEPSF113_4757
PFNHZ99_11940
PFOPfl01_0955(algG)
PFSPFLU_0985(algG)
PFCPflA506_0965(algG)
PPZH045_01665
PFBVO64_4066
PMANOU5_2452
PTVAA957_09070
PCGAXG94_10000
PVRPverR02_05200
PAZOAYR47_12205
POIBOP93_04710
PRXHRH33_05780
PMEDE3Z27_23145
PFWPF1751_v1c09430(algG)
PFFPFLUOLIPICF703415
PFXA7318_04265
PENPSEEN3463 PSEEN4540(algG)
PBAPSEBR_a4595
PBCCD58_24330
PPUUPputUW4_04476(algG)
PDRH681_19450
PSVPVLB_04210 PVLB_15520
PSKU771_06100
PKCPKB_0963(algG)
PCHEY04_04495
PCZPCL1606_50760
PCPJM49_25150
PFZAV641_18535
PLQAA042_15125
PALKPSAKL28_08690 PSAKL28_31910
PPVNJ69_13855
PSESPSCI_0726
PSEMTO66_05185
PSECCCOS191_0907(algG) CCOS191_2019
PPSYAOC04_15755
PSOSPOS17_1025
PKRAYO71_07515
PFKPFAS1_06355
PANRA7J50_0987
PSETTHL1_1263 THL1_3799
PSILPMA3_24610
PYMAK972_0833
PKEDLD99_05135
PALLUYA_04860 UYA_15375
PUMHGP31_04950
POJPtoMrB4_25890 PtoMrB4_46240(algG)
PGGFX982_00692
PGYAWU82_19105 AWU82_28980
PLALFXN65_05530
PSEJHNQ25_10115 HNQ25_19305
PVKEPZ47_24355
PEZHWQ56_05025
PBZGN234_23990
PKHJLK41_05195(algG)
PTYJWV26_01425 JWV26_15940(algG)
PATAJWU58_22775(algG)
PDWBV82_4695(algG)
PZEHU754_006245(algG)
PMOEHV782_005825(algG)
PCAMHNE05_19920
PSEPC4K39_1036
PVWHU752_027455(algG)
PTRTHU722_0006140(algG)
PXAKSS93_07765(algG)
PSAMHU731_020980(algG)
PTZHU718_005885(algG)
PQIKH389_10430 KH389_22270(algG)
PSHHHU773_022860(algG)
PXNHU772_004395(algG) HU772_009380
PHVHU739_012735(algG)
PPRGHU725_004060(algG) HU725_014450
PANHHU763_020355(algG)
PASGKSS96_05670(algG)
PFAKKSS94_04590(algG)
PMUYKSS95_07450(algG) KSS95_11710
PMAMKSS90_04810(algG) KSS90_09855
PAZEKSS91_23870(algG)
PALVKSS97_06270(algG)
PPHABVH74_05265
PORYEJA05_04775
PWZJ7655_04665(algG)
PTKEXN22_22980
PTWTUM18999_50170(algG)
PZAHU749_024165(algG)
PIEHU724_005645(algG)
PWYHU734_005455(algG)
PGFJ0G10_06050(algG)
PCAVD3880_05520 D3880_05585
PJUL1P09_20875(algG)
PHFNLY38_05215(algG)
PIZLAB08_R50460(algG)
PPEGKUA23_05125(algG)
PSIINF676_05320(algG)
PSIHLOY51_04680(algG)
AVNAvin_10920(algG)
AVLAvCA_10920(algG)
AVDAvCA6_10920(algG)
ACXAchr_31230(algG)
MSRAU15_13695
HMIsoil367_08840
ALKMNKI27_00770
CEGD0C16_10980
GILNHM04_15525
ABOABO_0394(algG)
ALNAS19_04070
GSNYC6258_00968
OMEOLMES_4482
TAUTola_1411 Tola_1417
REVHUE57_13025
PLGNCTC10937_04288(algG)
NISNIS_0188
CMEDFE773_08540
CPAFC6V80_01905
ARUIG6M88_17190
RHLLPU83_pLPU83d1240
MAADAZF01_02695
MMEDMame_02308(algG)
MLUTJET14_03745
MALGMALG_02780
YPACCEW88_18620
YANAYJ57_24975
TOMBWR18_20755
THAACFI11_06510
LSALKBK07_17235
SECHB18_00690
PARHI5S86_05455(algG)
SPINKV203_05825
FREFranean1_6675
FRIFraEuI1c_0157 FraEuI1c_0161
SENSACE_3182
SACGFDZ84_26120
PNDPla175_18190(algG)
CAOCelal_3935
CBALM667_16165
CBATM666_16150
TAMTheam_0793
NVNNVIE_010460
NEVNTE_02401
TAANMY3_01609(algG)
NFNNFRAN_2449(algG)
 » show all
Reference
1  [PMID:8144447]
  Authors
Franklin MJ, Chitnis CE, Gacesa P, Sonesson A, White DC, Ohman DE
  Title
Pseudomonas aeruginosa AlgG is a polymer level alginate C5-mannuronan epimerase.
  Journal
J Bacteriol 176:1821-30 (1994)
DOI:10.1128/JB.176.7.1821-1830.1994
  Sequence
[pae:PA3545]
Reference
2  [PMID:11707327]
  Authors
Morea A, Mathee K, Franklin MJ, Giacomini A, O'Regan M, Ohman DE
  Title
Characterization of algG encoding C5-epimerase in the alginate biosynthetic gene  cluster of Pseudomonas fluorescens.
  Journal
Gene 278:107-14 (2001)
DOI:10.1016/S0378-1119(01)00685-0
Reference
3  [PMID:14526115]
  Authors
Nyvall P, Corre E, Boisset C, Barbeyron T, Rousvoal S, Scornet D, Kloareg B, Boyen C
  Title
Characterization of mannuronan C-5-epimerase genes from the brown alga Laminaria  digitata.
  Journal
Plant Physiol 133:726-35 (2003)
DOI:10.1104/pp.103.025981
Reference
4  [PMID:12581364]
  Authors
Jain S, Franklin MJ, Ertesvag H, Valla S, Ohman DE
  Title
The dual roles of AlgG in C-5-epimerization and secretion of alginate polymers in Pseudomonas aeruginosa.
  Journal
Mol Microbiol 47:1123-33 (2003)
DOI:10.1046/j.1365-2958.2003.03361.x
Reference
5  [PMID:15968068]
  Authors
Douthit SA, Dlakic M, Ohman DE, Franklin MJ
  Title
Epimerase active domain of Pseudomonas aeruginosa AlgG, a protein that contains a right-handed beta-helix.
  Journal
J Bacteriol 187:4573-83 (2005)
DOI:10.1128/JB.187.13.4573-4583.2005
Reference
6  [PMID:24398681]
  Authors
Wolfram F, Kitova EN, Robinson H, Walvoort MT, Codee JD, Klassen JS, Howell PL
  Title
Catalytic mechanism and mode of action of the periplasmic alginate epimerase AlgG.
  Journal
J Biol Chem 289:6006-19 (2014)
DOI:10.1074/jbc.M113.533158
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 5.1.3.37
IUBMB Enzyme Nomenclature: 5.1.3.37
ExPASy - ENZYME nomenclature database: 5.1.3.37
BRENDA, the Enzyme Database: 5.1.3.37
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