KEGG ENZYME: 5.3.2.8

ENZYME: 5.3.2.8

Entry

EC 5.3.2.8                  Enzyme

Name

4-oxalomesaconate tautomerase;
GalD

Class

Isomerases;
Intramolecular oxidoreductases;
Interconverting keto- and enol-groups

Sysname

4-oxalomesaconate keto---enol-isomerase

Reaction(IUBMB)

(1E)-4-oxobut-1-ene-1,2,4-tricarboxylate = (1E,3E)-4-hydroxybuta-1,3-diene-1,2,4-tricarboxylate [RN:R07839]

Reaction(KEGG)

R07839

Substrate

(1E)-4-oxobut-1-ene-1,2,4-tricarboxylate [CPD:C04434]

Product

(1E,3E)-4-hydroxybuta-1,3-diene-1,2,4-tricarboxylate [CPD:C04324]

Comment

This enzyme has been characterized from the bacterium Pseudomonas putida KT2440 and is involved in the degradation pathway of syringate and 3,4,5-trihydroxybenzoate. It catalyses the interconversion of two of the tautomers of 4-oxalomesaconate, a reaction that can also occur spontaneously.

History

EC 5.3.2.8 created 2011 as EC 5.3.3.16, modified 2011, transferred 2012 to EC 5.3.2.8

Pathway

ec00362

Benzoate degradation

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K16514

4-oxalomesaconate tautomerase

Genes

KPN:

KPN_04047

KPU:

KP1_5416

KPM:	KPHS_52170

KPP:

A79E_0060

KPH:	KPNIH24_25955

KPZ:	KPNIH27_24940

KPV:	KPNIH29_25900

KPW:	KPNIH30_26295

KPY:	KPNIH31_25370

KPG:	KPNIH32_27160

KPC:	KPNIH10_25925

KPQ:	KPR0928_25440

KPT:	VK055_3418

KPO:	KPN2242_23315

KPR:

KPR_0060

KPJ:

N559_0096

KPI:	D364_20690

KPA:	KPNJ1_00063

KPS:	KPNJ2_00064

KPX:	PMK1_01553(galD)

KPB:	FH42_16625

KPNE:

KU54_000300

KPNU:

LI86_00300

KPNK:

BN49_0060

KVA:

Kvar_0052

KPE:

KPK_0046

KVD:	KR75_07865

KVQ:	SP68_14465

KMI:	VW41_14210

KQU:	AVR78_04135

KQV:	B8P98_00295

KAR:	LGL98_00285

KLM:	BWI76_14180

ROR:	RORB6_19055

RPLN:

B1209_00300

RAO:	DSD31_00335

CRO:

ROD_31661

CAMA:

F384_17455

CFAR:

CI104_02845

CTEL:

GBC03_28070

KAS:	KATP_00640

KLU:	K7B04_17495

IZH:	FEM41_02410

EBU:	CUC76_15890

SPE:

Spro_2090

SMAF:

D781_1917

SFG:	AV650_17155

SRZ:	AXX16_3212

SERM:

CLM71_09505

SQU:	E4343_05755

SFJ:	SAMEA4384070_2150(mii)

SRHZ:

FO014_00035

SENP:

KHA73_10600

RAH:	Rahaq_2669

RACE:

JHW33_03825

RAA:

Q7S_13295

ROX:	BV494_03500

RVC:	J9880_20315

RBON:

QNM34_13295

RIU:	I2123_10900

RBAD:

H2866_00175

RCB:	O1V66_04365

CARU:

P0E69_03200

NIG:	C1N62_02330

SERA:

Ser39006_020450

SERQ:

CWC46_20455

PBRA:

B5S52_08155

BGJ:	AWC36_13660

SOD:	Sant_P0186

EAM:	EAMY_1951(fldA3)

EAY:

EAM_1908

EBI:

EbC_05340

ETO:	RIN69_19420

PAO:	Pat9b_5774

KLN:	LH22_12875

PANS:

FCN45_11150

KPIE:

N5580_20050

PPHO:

CTZ24_22705

TCI:	A7K98_11230

GSS:	NYR30_00520

XCC:	XCC4031(fldA)

XCB:

XC_4120

XCA:	xcc-b100_4224

XCP:

XCR_0229

XCV:

XCV4254

XAX:

XACM_4026

XAC:	XAC4156(fldA)

XCI:	XCAW_00142(fldA)

XCT:	J151_04334

XCJ:	J158_04287

XCU:	J159_04283

XCN:	J169_04331

XCW:	J162_04289

XCR:	J163_04284

XCM:	J164_04284

XCF:	J172_04324

XFU:	XFF4834R_chr40260

XAO:	XAC29_20925

XOM:	XOO4300(XOO4300)

XOO:	XOO4563(fldA)

XOP:	PXO_03236(fldA)

XOY:	AZ54_24060

XOR:

XOC_0385

XOZ:	BE73_22475

XVE:	BJD12_11490

XPE:	BJD13_18245

XHR:	XJ27_14470

XGA:	BI317_01990

XPH:	XppCFBP6546_13625(XppCFBP6546P_13625)

XAN:	AC801_20680

XAR:	XB05_15695

XEU:	XSP_003901

XHD:	LMG31886_42480(ligU)

XPR:	MUG10_11445

XDY:	NYR95_21925

SRH:	BAY15_1810

SLM:	BIZ42_18430

DYG:	DYGSA30_06750(fldA)

LRZ:	BJI69_03980

LPY:	FIV34_03340

LAEG:

L2Y94_17960

LAEO:

L2Y97_03345

LAES:

L2Y96_03875

VNA:	PN96_18535

VEJ:	VEJY3_18101

VFL:	AL536_10300

VZI:	G5S32_20205

VOS:	KNV97_07820

GKD:	K6Q96_09570

PCQ:	PcP3B5_13880(galD)

PPU:	PP_2513(gllD)

PPF:	Pput_1360 Pput_3205

PPW:	PputW619_2026

PPB:	PPUBIRD1_3171

PPI:

YSA_00540

PPX:

T1E_3401

PPUT:

L483_20180

PPUN:

PP4_26410

PPSL:

BJP27_21275

PALD:

LU682_018055

PFL:

PFL_2685

PPRC:

PFLCHA0_c27490

PPRO:

PPC_2729

PFE:	PSF113_3478

PMUD:

NCTC8068_03359(mii)

PLUL:

FOB45_15895

PCP:	JM49_17375

PSOS:

POS17_2676(PFL_2685)

PSIL:

PMA3_18990

PEZ:	HWQ56_20090

PGF:	J0G10_16035

PIZ:	LAB08_R36500

PTAI:

ICN73_17180

PSJY:

AA098_11245

PFOU:

OZ911_17860

PSTT:

CH92_18155

AVN:	Avin_31290

AVL:	AvCA_31290

AVD:	AvCA6_31290

EMO:	DM558_04810

ENTO:

MTZ49_15280

PDEN:

F1C79_11720

ATN:	FM020_08435

SYK:	KDN34_01870

PCAR:

PC2016_2104

AAW:	AVL56_08880

ALR:	DS731_08765

PAT:

Patl_3889

ALII:

QR722_00510

MPAF:

R5R33_07660

CSA:

Csal_0328

MMW:	Mmwyl1_3941

MME:	Marme_0833

MPC:	Mar181_0381

MPRI:

MP3633_0449

MARD:

IBG28_02950

MFOI:

JSY38_12235

MPRF:

J8N69_06140

MRHI:

KDW99_17855

MPON:

MACH16_05300(fldA)

MGEO:

CFI10_14885

MARL:

HH196_02870

AJP:	AMJAP_1186(galD)

GSN:	YC6258_04098

RFO:	REIFOR_00530(prpF)

SALN:

SALB1_0066

NAQ:	D0T90_09285

NBL:	GJV52_09185

NWX:	CGZ65_09115

VIT:	ADP71_27500

VST:	LVJ81_00735

VMS:	LVJ82_10350

CHRO:

CXB49_01570

PSE:

NH8B_2727

AMAH:

DLM_2052 DLM_2160

AQS:	DKK66_18690

RSL:	RPSI07_mp1415

RSY:	RSUY_46200(galD)

RSG:	JK151_12000

RME:

Rmet_3706

BGF:	BC1003_5347

BUQ:	AC233_29265

BPH:

Bphy_3209

PGP:	CUJ91_26930

PCJ:	CUJ87_23800

PTRO:

G5S35_33695

PKF:	RW095_18410

PPK:	U875_22410

PPNO:

DA70_15090

PPNM:

LV28_15590

PRB:	X636_11755

PPUL:

RO07_09635

PSPU:

NA29_05550

PAPI:

SG18_10135

PVE:	UC34_01420

POX:	MB84_05755

PTX:	ABW99_00690 ABW99_15645

PFG:	AB870_12675

PNR:	AT302_09320

PAND:

DRB87_01285

PFIB:

PI93_001240

PCOM:

NTU39_01645

PLG:	NCTC10937_01989(mii_2)

HYF:	DTO96_100888(galD)

TVL:	FAZ95_13025

TCAR:

U0034_11230

BHO:

D560_0871

BHM:

D558_0857

BHZ:	ACR54_03617(galD)

BBRO:

BAU06_12660

BFZ:	BAU07_13775

BOH:	AKI39_11970

PUD:	G9Q38_04095

CDN:	BN940_08531

AFQ:

AFA_05545

AAQU:

D3M96_07085

PHN:	PAEH1_07135

OUE:	I6G29_08360

PIG:	EGT29_21015

PARK:

LSG25_04765

NARM:

N7E01_09385

PNA:

Pnap_2033

PVAC:

HC248_00679(galD)

ACID:

CBP33_05220

ACIP:

CBP36_05670

ACIN:

CBP34_05055

ACIS:

CBP35_13270

DAC:

Daci_4451

DEL:	DelCs14_2353

DTS:	BI380_01365

DHK:	BO996_20130

DLA:	I6G47_02105

AANT:

HUK68_11005

CENP:

M9799_00880

LIM:	L103DPR2_01491(galD_2)

PAIS:

PFX98_15215

HEE:	hmeg3_19340

HHF:	E2K99_06690

HEW:	HBDW_12240

MASW:

AM586_11750

MFY:	HH212_24610

MALI:

EYF70_25845

MUM:	FCL38_23505

MFLA:

GO485_19015

PLUE:

EWM63_10050

DUG:	HH213_29560

TMJ:	P0M04_30035

XYK:	GT347_25765

URU:	DSM104443_02502(ligU)

ARES:

IWH25_16400

ABRE:

pbN1_38990

APET:

ToN1_04080 ToN1_04220

AZO:	azo2544(fldA)

AOA:

dqs_2701

AZR:	CJ010_16090 CJ010_16430 CJ010_16495

THU:	AC731_016785

THAU:

C4PIVTH_0885(ligU)

TCL:

Tchl_1057

TAK:	Tharo_1098

AZQ:	G3580_09895 G3580_09965

ACOM:

CEW83_02940 CEW83_03000

MESR:

FGU64_09810

NIY:	FQ775_19485

NTU:	NTH_01141(galD)

MESO:

BSQ44_13010

CHEA:

PVE73_06160

AMIH:

CO731_01870(galD)

ORM:	HTY61_05170

SME:

SMc00497

SMK:	Sinme_1674

SMQ:	SinmeB_1519

SMX:	SM11_chr1630(fldA)

SMEG:

C770_GR4Chr1821

SMEL:

SM2011_c00497

SMER:

DU99_09680

SKM:	PZL22_002023

ALF:	CFBP5473_15980

AVV:	RvVAT039_pl06170 RvVAT039_pl06750

REI:	IE4771_PB00100

RLE:

RL2850

RHR:	CKA34_29830

RII:	FFM53_023765

RLS:	HB780_17725

RAW:	NE851_22935

ARA:

Arad_2914

NGL:	RG1141_CH37830

NGG:	RG540_CH38480

NEO:	CYG48_14360

NEN:	NCHU2750_16650

NPM:	QEO92_22235

RHT:	NT26_0688 NT26_3187

RFV:	RFYW14_03200

BSEI:

KMZ68_25460

RPA:	TX73_024400

RPB:

RPB_0878

RPD:

RPD_0988

RPE:

RPE_0745

RPT:

Rpal_5177

RPX:	Rpdx1_4871

RHOL:

RBJ75_17710

BVES:

QO058_09585

BOP:	AXW83_08325

BOI:	BLM15_26405

BOF:	FQV39_22230

BOW:	NWE53_16530

BOSE:

RMR04_28915

MTEZ:

HPT29_013255

RHJ:	HZY79_11975

PHL:

KKY_3765

PEM:	OF122_10960 OF122_18470

DEQ:	XM25_18675

DEA:	FPZ08_03370

YTI:	FNA67_00325

MAAD:

AZF01_20430

MLUT:

JET14_00620

BRN:	D1F64_00895 D1F64_21830

PPHR:

APZ00_03795

LAP:	ACP90_08350

LABT:

FIU93_13990(galD)

LAGG:

B0E33_20415

PSTG:

E8M01_14355

PAQT:

E8L99_01710

ACUT:

MRB58_02065

BSB:	Bresu_2120

BGOE:

IFJ75_11205

AEX:	Astex_2408

ASTD:

ATDW_23810

RUA:	D1823_11235

CBAK:

DA792_08285

MALG:

MALG_02282

YPAC:

CEW88_19485

YAN:	AYJ57_18380

SULZ:

C1J03_00900

SPOT:

G6548_05065

AHT:	ANTHELSMS3_00927 ANTHELSMS3_03530 ANTHELSMS3_03819

OCD:	FHY55_07540 FHY55_13935

ROT:	FIV09_09365(galD)

RMH:	LVO79_19415

MALU:

KU6B_25080(fldA) KU6B_53780

TGL:	HFZ77_06450

PALX:

GQA70_02095

RMAI:

MACH21_27830

PYE:	A6J80_20765

PARO:

CUV01_03965

PAMN:

JCM7685_2362

PPAN:

ESD82_14315

PLIA:

E4191_23195

PMAU:

CP157_03180(galD)

PAEX:

JHW48_10900

PALP:

JHW40_22765

PTP:	RCA23_c08340

DAA:	AKL17_0078

THW:	BMG03_15475

GFU:	KM031_19330

SALO:

EF888_06825 EF888_14025

SEDI:

EBB79_03585

PPSO:

QPJ95_03915

PSEB:

EOK75_20070

PSHQ:

F3W81_14645

PGV:	SL003B_2855

CACT:

HZ995_05385

CMAG:

CBW24_02310 CBW24_17060

NSM:	JO391_18025

AMAQ:

GO499_06020

PBAE:

P8S53_04780

RBG:	BG454_07490

NAR:

Saro_2818

NPP:	PP1Y_Mpl10392

NPN:	JI59_20865

NRE:	BES08_28955

NOV:	TQ38_025050 TQ38_028385

NOT:	C7W88_02295

NOR:	FA702_07580

NDR:	HT578_08995 HT578_09580

NHUM:

PQ457_10920

NOL:	NSDW_09510

NCP:	U0041_18325

SMAZ:

LH19_17720

SINA:

KNJ79_13880

RDI:	CMV14_06860

SPHI:

TS85_18490

SSAN:

NX02_17530

SPAN:

AWL63_08200

SKR:	BRX40_12215

SPHC:

CVN68_17270

SSUA:

FPZ54_06175

SBIN:

SBA_ch2_7360(fldA)

SGLC:

M1K48_07875

SHAN:

PPZ50_15760

SNOS:

K8P63_14345

SDON:

M9980_11070

SCH:	Sphch_3892

SSY:

SLG_12560

SPHB:

EP837_03486

SPHR:

BSY17_3452

SHYD:

CJD35_16795 CJD35_19545

SYA:	A6768_17505

SPYG:

YGS_C2P1465

SAMI:

SAMIE_1030470

SPAG:

sphantq_03936(ligU_1)

SPHG:

AZE99_04475

SFLA:

SPHFLASMR4Y_02982(galD)

SPHY:

CHN51_00925

BLAS:

BSY18_3518

BFW:	B5J99_08385

ACOB:

P0Y56_12140

SPHS:

ETR14_07955

POQ:	KZX46_07360

PMEA:

KTC28_03800

ALB:

AEB_P0248

AAY:	WYH_01903(galD)

ADO:	A6F68_01085(galD)

CMAN:

A9D14_17215

POT:	E2E27_18090

RFL:

Rmf_40810

ABS:	AZOBR_p310191

ABQ:	ABAZ39_27975

AZT:	TSH58p_32555

AARE:

D3093_31920

AZS:	E6C72_16015

NCB:	C0V82_22470

MAGX:

XM1_1086

MAGN:

WV31_13590

MAG:

amb0250

THAC:

CSC3H3_01150 CSC3H3_15795 CSC3H3_21875

THGO:

M9H61_18850

MAGQ:

MGMAQ_2664

NACI:

NUH88_13785

APB:	SAR116_0938

SNEA:

NBZ79_04475

GSO:	PH603_12815

AVP:	AVENP_2169(galD)

PARH:

I5S86_11470

MKN:	MKAN_15650

MSHG:

MSG_02375

MGO:	AFA91_01480

NTP:	CRH09_08940

ROA:	Pd630_LPD00179

SALB:

XNR_3396

SMA:	SAVERM_1616

SGRF:

SGFS_097130

SSX:	SACTE_0143

SVL:	Strvi_5288

SRC:	M271_45995

SMAL:

SMALA_0372

SSOI:

I1A49_05380

SFA:

Sfla_3395

STRP:

F750_3341(prpF)

SCW:	TU94_29780

SXI:	SXIM_52150

SCX:	AS200_08340

STRF:

ASR50_30565

SLE:	sle_08620(sle_08620)

SKY:	D0C37_14350

SGM:	GCM10017557_09430(fldA)

SPHW:

NFX46_13015

SLS:	SLINC_7291

STRD:

NI25_00245

SAUO:

BV401_05620

SNZ:	DC008_30120

SGE:	DWG14_01306(galD)

SGD:	ELQ87_04760

SCYA:

EJ357_41225

SAST:

CD934_02875

SFY:	GFH48_04165

SAQU:

EJC51_40085

SFUG:

CNQ36_03730

SCHA:

CP983_05325

SFEU:

IM697_27680

SVD:	CP969_30925

SPRA:

CP972_00295

SACT:

DMT42_32985

SLF:	JEQ17_07825

SRUG:

F0345_01625

SAKB:

K1J60_03920

SVIO:

HWN34_17310

STUB:

MMF93_31860

SSPN:

LXH13_34030

SCIR:

STRCI_006969

STEE:

F3L20_15140

SKG:	KJK29_03800

SCOA:

QU709_04030

SLUO:

PS467_36385

MAUR:

BOH66_06375

MLV:	CVS47_03113(galD)

MWA:	E4K62_17610

MPRT:

ET475_01800

MSED:

E3O41_03745

MCAW:

F6J84_14335

MLZ:	F6J85_16700

MICS:

C1N74_09930

MBIN:

LXM64_15070

MARK:

QUC20_09875

MINV:

T9R20_16035

MLIY:

RYJ27_09230

MICE:

EHF32_13605

MHOT:

PU630_12140

MCHN:

HCR76_14750

AARC:

G127AT_10880

AMIN:

AUMI_19420

AUM:	AURMO_01272

AUW:	AURUGA1_00416(galD)

AUT:	AINA4_04010(fldA)

LARI:

KI794_15060

LALL:

MUN78_00535

AJG:	KKR91_02695

ASUF:

MNQ99_16875

ACAO:

NF551_02620

AZX:	N2K95_02675

AJR:	N2K98_02555

ACH:

Achl_3362

PSNI:

NIBR502771_05720

PDEL:

JCQ34_19085

SATK:

SA2016_1318

CMUA:

P8192_03025

BKI:	M4486_03560

BSPO:

L1F31_00800

DCP:	RN607_02430

MUZ:	H4N58_01865

NAKE:

KGD83_17680

ACTW:

F7P10_28290

AMAZ:

LUW76_37660

ACIR:

OG979_33850

ACTQ:

OG417_31820

NOA:	BKM31_31875

NCX:	Nocox_11635(galD)

NGN:	LCN96_13380

NFS:	OIE67_33685

BSD:	BLASA_2605

BBRG:

U6N30_09235

MMAR:

MODMU_2828

AMQ:	AMETH_2205

AACD:

LWP59_32620

APRT:

MUY14_18905

ATHM:

L1857_13805

ARHD:

VSH64_11520

AMOG:

QRX60_32785

PSEE:

FRP1_23145

PSEQ:

AD006_02330

PECQ:

AD017_10155

PHH:	AFB00_07655

KUT:	JJ691_36310

PMAD:

BAY61_21980

UME:	RM788_49945

ACTN:

L083_3806

AIH:	Aiant_15050(fldA)

ACTL:

L3i22_074690 L3i22_080320

ASIC:

Q0Z83_006200 Q0Z83_063570

AOU:	ACTOB_006185

ACTE:

ACTI_78060 ACTI_82270

DACT:

KZZ52_32675

JTL:	M6D93_14100

BALA:

DSM104299_05098(ligU)

AFLO:

HEQ12_08575

SKV:	NIES73_25400(ligO)

VAB:	WPS_31630(fldA)

PPHT:

GA004_04615

SLI:

Slin_4025

SPIB:

G8759_04365

EOL:	Emtol_3451

RSI:	Runsl_1543

RUN:	DR864_16580

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Reference

1 [PMID:21219457]

Authors

Nogales J, Canales A, Jimenez-Barbero J, Serra B, Pingarron JM, Garcia JL, Diaz E

Title

Unravelling the gallic acid degradation pathway in bacteria: the gal cluster from Pseudomonas putida.

Journal

Mol Microbiol 79:359-74 (2011)
DOI:10.1111/j.1365-2958.2010.07448.x

Sequence

[ppu:PP_2513]

Other DBs

ExplorEnz - The Enzyme Database:

5.3.2.8

IUBMB Enzyme Nomenclature:

5.3.2.8

ExPASy - ENZYME nomenclature database:

5.3.2.8

BRENDA, the Enzyme Database:

5.3.2.8

LinkDB

DBGET integrated database retrieval system