KEGG   ENZYME: 5.4.99.3
Entry
EC 5.4.99.3                 Enzyme                                 
Name
2-acetolactate mutase;
acetolactate mutase;
acetohydroxy acid isomerase
Class
Isomerases;
Intramolecular transferases;
Transferring other groups
Sysname
2-acetolactate methylmutase
Reaction(IUBMB)
2-acetolactate = 3-hydroxy-3-methyl-2-oxobutanoate [RN:R03052]
Reaction(KEGG)
R03052 > R05071;
(other) R05069
Substrate
2-acetolactate [CPD:C00900]
Product
3-hydroxy-3-methyl-2-oxobutanoate [CPD:C04181]
Comment
Requires ascorbic acid; also converts 2-aceto-2-hydroxybutanoate to 3-hydroxy-3-methyl-2-oxopentanoate.
History
EC 5.4.99.3 created 1972
Pathway
ec00290  Valine, leucine and isoleucine biosynthesis
ec01110  Biosynthesis of secondary metabolites
Reference
1  [PMID:4384955]
  Authors
Allaudeen HS, Ramakrishnan T.
  Title
Biosynthesis of isoleucine and valine in Mycobacterium tuberculosis H37 Rv.
  Journal
Arch Biochem Biophys 125:199-209 (1968)
DOI:10.1016/0003-9861(68)90655-3
Other DBs
ExplorEnz - The Enzyme Database: 5.4.99.3
IUBMB Enzyme Nomenclature: 5.4.99.3
ExPASy - ENZYME nomenclature database: 5.4.99.3
BRENDA, the Enzyme Database: 5.4.99.3
CAS: 37318-52-8
LinkDB

KEGG   REACTION: R05069
Entry
R05069                      Reaction                               
Name
(S)-2-aceto-2-hydroxybutanoate:NADP+ oxidoreductase (isomerizing)
Definition
(S)-2-Aceto-2-hydroxybutanoate <=> (R)-3-Hydroxy-3-methyl-2-oxopentanoate
Equation
C06006 <=> C14463
Reaction class
RC01726  C06006_C14463
Enzyme
1.1.1.86        5.4.99.3
Pathway
rn00290  Valine, leucine and isoleucine biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
rn01210  2-Oxocarboxylic acid metabolism
rn01230  Biosynthesis of amino acids
Module
M00019  Valine/isoleucine biosynthesis, pyruvate => valine / 2-oxobutanoate => isoleucine
M00570  Isoleucine biosynthesis, threonine => 2-oxobutanoate => isoleucine
Brite
Enzymatic reactions [BR:br08201]
 1. Oxidoreductase reactions
  1.1  Acting on the CH-OH group of donors
   1.1.1  With NAD+ or NADP+ as acceptor
    1.1.1.86
     R05069  (S)-2-Aceto-2-hydroxybutanoate <=> (R)-3-Hydroxy-3-methyl-2-oxopentanoate
 5. Isomerase reactions
  5.4  Intramolecular transferases
   5.4.99  Transferring other groups
    5.4.99.3
     R05069  (S)-2-Aceto-2-hydroxybutanoate <=> (R)-3-Hydroxy-3-methyl-2-oxopentanoate
Orthology
K00053  ketol-acid reductoisomerase [EC:1.1.1.86]
Other DBs
RHEA: 25407
LinkDB

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