KEGG   ENZYME: 6.2.1.69
Entry
EC 6.2.1.69                 Enzyme                                 
Name
L-cysteine---[L-cysteinyl-carrier protein] ligase;
pchE (gene name);
pchF (gene name);
angR (gene name)
Class
Ligases;
Forming carbon-sulfur bonds;
Acid-thiol ligases
Sysname
L-cysteine:[L-cysteinyl-carrier protein] ligase (AMP-forming)
Reaction(IUBMB)
ATP + L-cysteine + holo-[L-cysteinyl-carrier protein] = AMP + diphosphate + L-cysteinyl-[L-cysteinyl-carrier protein] (overall reaction);
(1a) ATP + L-cysteine = diphosphate + (L-cysteinyl)adenylate [RN:R12765];
(1b) (L-cysteinyl)adenylate + holo-[L-cysteinyl-carrier protein] = AMP + L-cysteinyl-[L-cysteinyl-carrier protein]
Reaction(KEGG)
R12765;
(other) R12766 R12767
Substrate
ATP [CPD:C00002];
L-cysteine [CPD:C00097];
holo-[L-cysteinyl-carrier protein];
(L-cysteinyl)adenylate [CPD:C22385]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
L-cysteinyl-[L-cysteinyl-carrier protein];
(L-cysteinyl)adenylate [CPD:C22385]
Comment
The adenylation domain of the enzyme catalyses the activation of L-cysteine to (L-cysteinyl)adenylate, followed by the transfer of the activated compound to the free thiol of a phosphopantetheine arm of a peptidyl-carrier protein domain. The peptidyl-carrier protein domain may be part of the same protein, or of a different protein. This activity is often found as part of a larger non-ribosomal peptide synthase.
History
EC 6.2.1.69 created 2021
Pathway
ec01053  Biosynthesis of siderophore group nonribosomal peptides
Orthology
K12239  L-cysteine---[L-cysteinyl-carrier protein] ligase PchE
K12240  L-cysteine---[L-cysteinyl-carrier protein] ligase PchF
Genes
KMI: VW41_14180
PGE: LG71_13430 LG71_26195
SYM: K6K13_09575
YPE: YPO0776
YPA: YPA_3374
YPN: YPN_3216
YPM: YP_3425(entF3)
YPZ: YPZ3_0722
YPT: A1122_01155
YPD: YPD4_0678
YPX: YPD8_0678
YPW: CH59_1087
YPJ: CH55_3520
YPV: BZ15_2798
YPL: CH46_140
YPS: YPTB3296
YPO: BZ17_3313
YPI: YpsIP31758_0684
YPY: YPK_0752
YPB: YPTS_3434
YPQ: DJ40_3145
YPU: BZ21_2577
YPR: BZ20_2822
YPC: BZ23_2860
YPF: BZ19_2700
YSI: BF17_03615
YFR: AW19_774
YKR: CH54_888
YEG: PL78_04165
PCAC: OI450_11540
PAO: Pat9b_4773
PTT: VY86_04720
XBV: XBW1_1484
LANH: KR767_17535
PAE: PA4225(pchF) PA4226(pchE)
PAEV: N297_4357 N297_4358
PAEI: N296_4357 N296_4358
PAU: PA14_09270(pchE) PA14_09280(pchF) PA14_54920
PAP: PSPA7_0874(pchE) PSPA7_0875(pchF)
PAG: PLES_07011(pchE) PLES_07021(pchF)
PAF: PAM18_0711(pchE) PAM18_0712(pchF)
PNC: NCGM2_5470(pchF) NCGM2_5471(pchE)
PAEB: NCGM1900_0733(pchE) NCGM1900_0734(pchF)
PDK: PADK2_03540 PADK2_03545
PSG: G655_03575 G655_03580
PRP: M062_22260 M062_22265
PAEP: PA1S_03690 PA1S_03695
PAER: PA1R_gp2116 PA1R_gp2117
PAEM: U769_03720 U769_03725
PAEL: T223_03620 T223_03625
PAES: SCV20265_0735 SCV20265_0736
PAEU: BN889_04699(pchF) BN889_04700(pchE_1) BN889_04701(pchE_2)
PAEG: AI22_00215 AI22_00220
PAEC: M802_4355 M802_4356
PAEO: M801_4223 M801_4224
PPAA: B7D75_03630
PFL: PFL_3492(pchF) PFL_3493(pchE)
PPRC: PFLCHA0_c35330(irp1) PFLCHA0_c35340(irp2)
PPRO: PPC_3646(pchF) PPC_3647(pchE)
PFB: VO64_5522
PVR: PverR02_15520 PverR02_15525
PAZO: AYR47_21960
PFW: PF1751_v1c25800
PFF: PFLUOLIPICF724130
PCZ: PCL1606_27860 PCL1606_27870
PSEM: TO66_16985 TO66_16990
PSOS: POS17_3635(pchF) POS17_3636(pchE)
PSEP: C4K39_5485 C4K39_5486
PSAM: HU731_027795
PZA: HU749_012685
EMO: DM558_05695
PSEN: PNC201_20480(mbtB)
MII: BTJ40_11110
MEC: Q7C_386
MPUR: MARPU_03050
SAJS: QO259_16200 QO259_16205
ADI: B5T_02512
CHRO: CXB49_08490
AQL: BXU06_11810
MAER: DAI18_17600
RSO: RSc1811
RSE: F504_1578
RPU: CDC45_09320
RNC: GO999_08685
RSL: RPSI07_1867
RSM: CMR15_11817
BPS: BPSS0586(pchE) BPSS0587(pchF)
BPM: BURPS1710b_A2150(pchE) BURPS1710b_A2151(pchF)
BPL: BURPS1106A_A0787 BURPS1106A_A0788
BPD: BURPS668_A0877 BURPS668_A0878
BPSE: BDL_3815 BDL_3816
BPSM: BBQ_5606 BBQ_5607
BPSU: BBN_3987 BBN_3988
BPSD: BBX_5652 BBX_5653
BPZ: BP1026B_II0646(pchE) BP1026B_II0647(pchF)
BPQ: BPC006_II0832 BPC006_II0833
BPK: BBK_3903 BBK_3904
BPSH: DR55_4763 DR55_4764
BPSA: BBU_5458 BBU_5459
BPSO: X996_3767 X996_3768
BUT: X994_4031 X994_4032
BTE: BTH_II1827 BTH_II1828(pchE)
BTQ: BTQ_5111 BTQ_5112
BTJ: BTJ_3739 BTJ_3740
BTZ: BTL_4597 BTL_4598
BTV: BTHA_5631 BTHA_5632
BTHE: BTN_4236 BTN_4237
BTHM: BTRA_3856 BTRA_3857
BTHA: DR62_4285 DR62_4286
BTHL: BG87_4568 BG87_4569
BUR: Bcep18194_B0672 Bcep18194_B0673
BCM: Bcenmc03_5289 Bcenmc03_5290
BCH: Bcen2424_4997 Bcen2424_4998
BCJ: BCAM2230(pchE)
BCEO: I35_6120(pchF) I35_6121(pchE)
BCED: DM42_5764 DM42_5765
BCEP: APZ15_27910 APZ15_27915
BSEM: WJ12_31050 WJ12_31055
BSTL: BBJ41_24870 BBJ41_24875
BARI: NLX30_26105 NLX30_26110
BGD: bgla_4p2140
BGP: BGL_1c20570(pchF) BGL_1c20580(pchE)
BPLA: bpln_1g19090 bpln_1g19100
OTD: J1M35_10305
HRB: Hrubri_2237
BRK: CWS35_16345
RPC: RPC_3032
SECH: B18_04270 B18_04275
ALI: AZOLI_p20160(pchE) AZOLI_p20161(pchF)
HJO: AY555_10640
DEO: CAY53_00285
SCU: SCE1572_43415
LYB: C3943_18865
LPAK: GDS87_10285
LMAC: I6G82_03570
BLR: BRLA_c037020
BFM: BP422_17845
BAGR: BA6348_08945
BRW: GOP56_10725
BPAB: PSE45_20770
SANC: SANR_1291
FHM: R0V13_03420
CKL: CKL_1511
CKR: CKR_1405
CSB: CLSA_c16940(irp2)
CLD: CLSPO_c23060
CAUN: CLAUR_026160(dltA_6)
RPAY: P0092_11085 P0092_11095
RAL: Rumal_0885
DSY: DSY2821
DHD: Dhaf_3965
DAE: Dtox_2674
BPRO: PMF13cell1_00657(mbtB_1)
ACAC: EYQ97_15755
EHL: EHLA_1441
RTO: RTO_11580 RTO_12320
CBOL: CGC65_09170
SINT: K5I26_14510
VGU: HYG85_16695
THYR: P4S50_07140
AVG: I6H45_03220
PFT: JBW_03609 JBW_03612
MANA: MAMMFC1_02828(mbtB_2)
STED: SPTER_12030(mbtB_1)
EYY: EGYY_00410(EntF) EGYY_29280(EntF)
AEQ: AEQU_0072 AEQU_0074
AHAT: ADCFC_02020
MPA: MAP_3742
MAVI: RC58_00135
MAVU: RE97_00135
MPAG: C0J29_21250
CDH: CDB402_1084(irp2C)
CDZ: CD31A_2083(irp2C)
CDS: CDC7B_2042(irp2C)
CDD: CDCE8392_1964(irp2C)
CDIP: ERS451417_02094(irp2C)
CSX: CSING_01210(irp2C)
CSP: WM42_1816
NFR: ERS450000_00763(mbtB_1)
NCY: NOCYR_2585
NNO: NONO_c49320
NOD: FOH10_15025
NWL: NWFMUON74_24310
NIE: KV110_22875 KV110_22880 KV110_35845
CBQ: AL705_04305
SCO: SCO7683(SC4C2.18)
SCYE: R2B67_00090
SCB: SCAB_1471 SCAB_1481
SSX: SACTE_0740
SRC: M271_09930 M271_09960
SVE: SVEN_0512 SVEN_0517
SLV: SLIV_00885
SGU: SGLAU_04465(nrps1B) SGLAU_04470(nrps1C) SGLAU_04640(nrps1D) SGLAU_04650(nrps1E)
STRE: GZL_p00202
SPHW: NFX46_19160 NFX46_19185
SLS: SLINC_1332
STRD: NI25_03970
SNQ: CP978_29875
SPAD: DVK44_09635 DVK44_09640
SPAC: B1H29_04830
SHUN: DWB77_06824(mbtB_1)
SHAR: HUT13_23935
SFEU: IM697_24270
SROI: IAG44_38705
SDUR: M4V62_41900
STUB: MMF93_04755
SYUN: MOV08_35385
SCOA: QU709_01700
YIA: LO772_01875
CMI: CMM_0330(npsB)
CMH: VO01_01490
CMC: CMN_00301(npsB)
CCAF: FGD68_03585
HMM: R3I40_10080
DCO: SAMEA4475696_1592(mbtB_3)
PACD: EGX94_02945
PAUS: NCTC13651_00740(mbtB_1)
THAO: NI17_005435
TALX: FOF52_04705
NDA: Ndas_1030 Ndas_1761
NEX: NE857_12315
NAKE: KGD83_20620
NCG: KGD84_12930
SNAH: OUQ99_18135
ACTW: F7P10_30275
ACTQ: OG417_42820
SRO: Sros_3767
SACG: FDZ84_24490
SACC: EYD13_12025(mbtB1)
AORI: SD37_07330 SD37_10150
PHH: AFB00_19555
PAUT: Pdca_37550
KBU: Q4V64_43930
AHM: TL08_14435
ACTU: Actkin_03287(angR)
CROS: N8J89_28075
STP: Strop_2820
VMA: VAB18032_07015
MCRA: ID554_27815
VER: HUT12_19180
MFEU: H1D33_21235
ASE: ACPL_6139(nrps2A) ACPL_6158(nrps2C)
ACTS: ACWT_6007 ACWT_6026
AOU: ACTOB_004298 ACTOB_004317
PRY: Prubr_62260
AMAS: QU670_12580
 » show all
Reference
1  [PMID:10555976]
  Authors
Quadri LE, Keating TA, Patel HM, Walsh CT
  Title
Assembly of the Pseudomonas aeruginosa nonribosomal peptide siderophore pyochelin: In vitro reconstitution of aryl-4, 2-bisthiazoline synthetase activity from PchD, PchE, and PchF.
  Journal
Biochemistry 38:14941-54 (1999)
DOI:10.1021/bi991787c
  Sequence
[pau:PA14_09270 PA14_09280]
Other DBs
ExplorEnz - The Enzyme Database: 6.2.1.69
IUBMB Enzyme Nomenclature: 6.2.1.69
ExPASy - ENZYME nomenclature database: 6.2.1.69
BRENDA, the Enzyme Database: 6.2.1.69
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