KEGG   ENZYME: 6.3.1.15
Entry
EC 6.3.1.15                 Enzyme                                 
Name
8-demethylnovobiocic acid synthase;
novL (gene name);
novobiocin ligase;
novobiocic acid synthetase (misleading);
8-desmethyl-novobiocic acid synthetase;
8-demethylnovobiocic acid synthetase;
3-dimethylallyl-4-hydroxybenzoate:3-amino-4,7-dihydroxycoumarin ligase (AMP-forming)
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-ammonia (or amine) ligases (amide synthases)
Sysname
4-hydroxy-3-prenylbenzoate:3-amino-4,7-dihydroxycoumarin ligase (AMP-forming)
Reaction(IUBMB)
ATP + 4-hydroxy-3-prenylbenzoate + 3-amino-4,7-dihydroxycoumarin = AMP + diphosphate + 8-demethylnovobiocic acid [RN:R10453]
Reaction(KEGG)
R10453
Substrate
ATP [CPD:C00002];
4-hydroxy-3-prenylbenzoate [CPD:C12458];
3-amino-4,7-dihydroxycoumarin [CPD:C12468]
Product
AMP [CPD:C00020];
diphosphate [CPD:C00013];
8-demethylnovobiocic acid [CPD:C20629]
Comment
The enzyme is involved in the biosynthesis of the aminocoumarin antibiotic novobiocin.
History
EC 6.3.1.15 created 2013
Pathway
ec00401  Novobiocin biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K12709  8-demethylnovobiocic acid synthase
Genes
SANL: KZO11_24540
STUI: GCM10017668_29390
SHK: J2N69_02925
SDD: D9753_06660
SLON: LGI35_32545
SCAJ: O1G22_18810
NGV: CDO52_02200
ASIC: Q0Z83_043110
Reference
1  [PMID:10801869]
  Authors
Steffensky M, Li SM, Heide L
  Title
Cloning, overexpression, and purification of novobiocic acid synthetase from Streptomyces spheroides NCIMB 11891.
  Journal
J Biol Chem 275:21754-60 (2000)
DOI:10.1074/jbc.M003066200
  Sequence
[ag:AAF67505]
Reference
2  [PMID:15218104]
  Authors
Pi N, Meyers CL, Pacholec M, Walsh CT, Leary JA
  Title
Mass spectrometric characterization of a three-enzyme tandem reaction for assembly and modification of the novobiocin skeleton.
  Journal
Proc Natl Acad Sci U S A 101:10036-41 (2004)
DOI:10.1073/pnas.0403526101
  Sequence
[ag:AAF67505]
Reference
3  [PMID:16274243]
  Authors
Pacholec M, Tao J, Walsh CT
  Title
CouO and NovO: C-methyltransferases for tailoring the aminocoumarin scaffold in coumermycin and novobiocin antibiotic biosynthesis.
  Journal
Biochemistry 44:14969-76 (2005)
DOI:10.1021/bi051599o
Other DBs
ExplorEnz - The Enzyme Database: 6.3.1.15
IUBMB Enzyme Nomenclature: 6.3.1.15
ExPASy - ENZYME nomenclature database: 6.3.1.15
BRENDA, the Enzyme Database: 6.3.1.15
LinkDB

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