Entry
Name
coenzyme F420-0:L-glutamate ligase;
CofE-AF;
MJ0768;
CofE
Class
Ligases;
Forming carbon-nitrogen bonds;
Acid-D-amino-acid ligases (peptide synthases)
BRITE hierarchy
Sysname
L-glutamate:coenzyme F420-0 ligase (GDP-forming)
Reaction(IUBMB)
GTP + coenzyme F420-0 + L-glutamate = GDP + phosphate + coenzyme F420-1 [RN:
R09399 ]
Reaction(KEGG)
Substrate
Product
Comment
This protein catalyses the successive addition of two glutamate residues to cofactor F420 by two distinct and independent reactions. In the reaction described here the enzyme attaches a glutamate via its alpha-amine group to F420-0. In the second reaction (EC
6.3.2.34 , coenzyme F420-1---gamma-L-glutamate ligase) it catalyses the addition of a second L-glutamate residue to the gamma-carboxyl of the first glutamate.
History
EC 6.3.2.31 created 2010
Pathway
ec01120 Microbial metabolism in diverse environments
Orthology
K12234 coenzyme F420-0:L-glutamate ligase / coenzyme F420-1:gamma-L-glutamate ligase
K24998 dehydro coenzyme F420 reductase / coenzyme F420-0:L-glutamate ligase / coenzyme F420-1:gamma-L-glutamate ligase
Genes
NRE : BES08_02705 BES08_26370
SCH : Sphch_2140 Sphch_2202
SPHJ : BSL82_04905 BSL82_08910
MMAE : MMARE11_12460(fbiB)
MBAI : MB901379_01171(fbiB)
MHEK : JMUB5695_03655(fbiB)
MPHL : MPHLCCUG_01626(fbiB_3)
MSAL : DSM43276_03406(fbiB_2)
MTER : 4434518_02998(fbiB)
NWL : NWFMUON74_13470(fbiB)
RKO : JWS14_25820 JWS14_34320
TPUL : TPB0596_34400(fbiB)
SHO : SHJGH_4267 SHJGH_5926
SAMB : SAM23877_3079(fbiB)
SLE : sle_42440(sle_42440)
SGM : GCM10017557_50170(fbiB)
SDX : C4B68_06930 C4B68_24085
SPAD : DVK44_11200 DVK44_18510
SGF : HEP81_01492 HEP81_04779
STUI : GCM10017668_27350(fbiB)
SHUN : DWB77_04646(fbiB_2)
KAB : B7C62_12015 B7C62_12850
AMAU : DSM26151_28160(fbiB_1) DSM26151_28190(fbiB_2)
NAQU : ENKNEFLB_03315(fbiB)
NEX : NE857_28265 NE857_32350(cofE)
ACTI : UA75_23080 UA75_27205
ACAD : UA74_22560 UA74_26615
ACTU : Actkin_05520(fbiB_3)
STP : Strop_0943 Strop_1139 Strop_1147
MSAG : GCM10017556_35200(fbiB)
SBAE : DSM104329_01861(fbiB_1)
MPD : MCP_1297(cofE-1) MCP_2474(cofE-2)
HSU : HLASF_0415 HLASF_1128
HSF : HLASA_0414 HLASA_1117
HHI : HAH_2869(cofE) HAH_4273
HHN : HISP_14585 HISP_16585
HAB : SG26_00985 SG26_06725
HDS : HSR122_0386(cofE) HSR122_2881(cofE2)
HAE : halTADL_0320 halTADL_1345
HDF : AArcSl_2462(cofE) AArcSl_2524(cofE2)
LOKI : Lokiarch_23940(cofE_1) Lokiarch_46970(cofE_3)
» show all
Taxonomy
Reference
Authors
Li H, Graupner M, Xu H, White RH
Title
CofE catalyzes the addition of two glutamates to F420-0 in F420 coenzyme biosynthesis in Methanococcus jannaschii.
Journal
Sequence
Reference
Authors
Nocek B, Evdokimova E, Proudfoot M, Kudritska M, Grochowski LL, White RH, Savchenko A, Yakunin AF, Edwards A, Joachimiak A
Title
Structure of an amide bond forming F(420):gamma-glutamyl ligase from Archaeoglobus fulgidus -- a member of a new family of non-ribosomal peptide synthases.
Journal
Sequence
Other DBs
ExplorEnz - The Enzyme Database: 6.3.2.31
ExPASy - ENZYME nomenclature database: 6.3.2.31
LinkDB
All DBs